264,819 research outputs found
Wave disturbance behind low crested structures:diffraction and overtopping
No full textIn recent years the hydraulic performance of low-crested structures has been widely studied both theoretically and through experimental analyses, but only a few studies have been focused on the combined diffraction-overtopping effects on wave transmission and induced wave currents.In this paper hydraulic model tests conducted at the 3D wave basin of Delft University of Technology (Caceres et al., 2008) were used to obtain and discuss two simple methods for predicting the wave height at the lee of a single detached breakwater of finite length and the related current regime. For the first time diffraction effects are expressly accounted for. The agreement with experimental data is encouraging. The main objective of the paper is to aid engineers in the first stage of the design process, when using a mathematical model could be unnecessarily excessive. (C) 2009 Elsevier B.V. All rights reserved
Learning Flamenco in Caceres
No full text13 p.Dance may be one of the oldest art forms in the world, and it is a type of expression that I believe can provide valuable insight into the culture that created it. Therefor I choose to investigate the art of flamenco dancing for my Integrative Cultural Research Project (JCRP) in Caceres, Spain.
To study flamenco, I enrolled in a "basic flamenco" class at the Sbelta school of dance here in Caceres. The project coordinator helped me locate the school and put me in contact with the directer. The class I elected was held every Monday and Wednesday from 8:00 to 9:00 PM. and I would also go on occasional Fridays to learn the parts of the dances that the other students already knew. I would participate in the class and, afterwards, write notes describing what we had learned, how the class was conducted, and my personal reactions and experience
Design and development of a 3D PC video game with simulation construction and real-time resource management
No full textTreball final de Grau en Disseny i Desenvolupament de Videojocs. Codi: VJ1241. Curs: 2021-2022The objective of this Technical Report is to present the project that has been developed in the context
of the subject VJ1241 - Final Degree Project. Specifically, this work focuses on the design and
development of a 3D video game for personal computer (PC) in which the player assumes the role of a
construction company manager.
The player will be able to obtain construction contracts and will manage these works taking into
account different important elements of construction.
The key to success in the game is to carry out the works in the city without any inconvenience and to
achieve a positive reputation in the city.
The novelties that I am trying to propose are the management of construction resources in real time,
simulating the real management that exists on the construction site and its drawbacks.
The results have been better than I thought and have fulfilled my expectations. It has been a very
rewarding experience
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Azorella compacta (Yareta):an endemic plant of the Andean planteaux in South America. Azorella compacta (Yareta): pianta endemica degli altipiani andini del Sud America
No full textAzorella compacta (Yareta): pianta endemica degli altipiani andini del Sud America.
– Si riportano i risultati di una ricerca sugli usi della Azorella compacta Phil da parte delle popolazioni sudamericane del Perù, Argentina, Cile e Bolivia, allo scopo di realizzare una documentazione etnobotanica di partenza per possibili applicazioni pratiche della pianta. La sperimentazione sulla A. compacta ha preso in esame lo stato attuale di conservazione, la classificazione e descrizione botanica, l’ecologia, la distribuzione, l’utilizzazione in etnomedicina e etnofarmacologia e altre possibili impieghi.
Dalla suddetta esperienza è emerso che tutte le comunità in studio,dei suddetti Paesi, utilizzano A. compacta come combustibile, come medicina e raramente come biocida; per tali scopi viene principalmente impiegata la resina, estratta dalla pianta, come impiastro per curare principalmente i danni al sistema osseo e muscolare quali contusioni, fratture e reumatismi.Azorella compacta (Yareta): endemic to the Andean plateaux of South America.
The results of a research on the uses of Azorella compacta Phil (popular name: Yareta) among the populations of Peru, Argentina, Chile and Bolivia, with the aim of providing an ethno-botanical documentation as a basis for possible pratctical applications of such plant. With this aim, A. compacta has been examined taking into account its present state of preservation, botanical characteristics, ecology, distribution, ethno-medical and ethno-pharmacological uses, and other possible uses.
The study has shown that in all of these countries, A. compacta is used as combustible, medicinal and biocide purposes; The resin It is extracted from the plant is primarily used. as poultice to treat mainly damage to bone and muscular system such as bruises, fractures and rheumatism
Le cactacee presenti in Yura: tassonomia, ecologia e distribuzione. Taxonomy,, ecology and distribution of the cactaceae species in Yura (Arequipa, Perù)
No full text
In vitro activity of five medicinal plants commonly used in Guatemala and some of their fractions against the Epimastigote and Trypomastigote form of Trypanosoma cruzi.
No full textIdentification of in vivo RNA tragets of the RNA-binding proteins Acinus and hnRNP A1
No full textRNA-binding proteins play a central role in the post-transcriptional regulation of gene expression; however, little is known about the endogenous transcripts to which they bind. Here, I have used the ultra-violet cross-linking and immuno-precipitation (CLIP) technique to identify RNA targets directly bound to two RNA-binding proteins: Acinus and hnRNP A1.
Acinus (apoptotic chromatin condensation inducer in the nucleus) contains a region that is homologous to the RNA binding domain of the Drosophila splicing regulator sex-lethal, and a serine and arginine rich region similar to that seen in the SR family of proteins, which function extensively in splicing. Furthermore it is a component of the multi-protein spliceosome complex, and I have demonstrated it can directly bind polyadenylated RNA. I have shown that Acinus displays a diffuse nuclear localisation pattern, however, overexpression of an epitope-tagged protein results in its accumulation in enlarged nuclear speckles. Together these results suggest a role in pre-mRNA splicing.
Acinus is cleaved during apoptosis by caspase-3, resulting in a truncated protein with chromatin condensation inducing activity (Sahara et al., 1999). Accordingly, I have demonstrated that overexpression of epitope-tagged Acinus results in an increased number of cells exhibiting an apoptotic phenotype. The proteolytic fragment contains the RNA binding region, and to determine if the role of Acinus in apoptosis is mediated by RNA interactions I utilised CLIP to identify in vivo RNA targets.
I have identified several mRNA targets of Acinus and found that the binding sites in those mRNA targets predominantly map to constitutively expressed exons. This is in agreement with the exon junction complex, of which Acinus is a component, being deposited on mRNAs after splicing. These results may indicate that Acinus is a core RNA binding factor of the exon junction complex.
To complement this approach, I also performed CLIP with a known alternative splicing regulator, hnRNP A1. In this manner, the binding site preferences could be compared between the two proteins. As expected, the majority of hnRNP A1 binding sites are located in introns, corresponding with their identified role of antagonizing pre-mRNA splicing by binding intronic splicing elements. Interestingly, a number of the CLIP tags are located in, or adjacent to, alternatively spliced events suggesting a role for hnRNP A1 in the regulation of alternative splicing of these specific pre-mRNAs. In addition to pre-mRNA splicing hnRNP A1 also functions in the cellular stress response. Upon environmental stresses it relocates to the cytoplasm and accumulates in cytoplasmic foci known as stress granules (Guil et al., 2006). Here I show some of the targets identified by CLIP are regulated by hnRNP A1 in times of cellular stress.
In summary, I have identified two novel subsets of RNAs, bound by Acinus or hnRNP A1 in vivo. I have shown these proteins exhibit distinct binding preferences, which correspond to their biological function. This work is consistent with hnRNP A1 acting as an alternative splicing regulator, and provides evidence for a dual role of Acinus in mRNA splicing and apoptosis. This study also demonstrates the power of the CLIP technique, as identification of in vivo RNA targets allows greater understanding of the mechanisms by which RNA-binding proteins exert their regulatory control
Wave energy and wave-induced flow reduction by full-scale model Posidonia oceanica seagrass
No full textThis paper presents results from experiments in a large flume on wave and flow attenuation by a full-scale artificial Posidonia oceanica seagrass meadow in shallow water. Wave height and in-canopy wave-induced flows were reduced by the meadow under all tested regular and irregular wave conditions, and were affected by seagrass density, submergence and distance from the leading edge. The energy of irregular waves was reduced at all components of the spectra, but reduction was greater at the peak spectral frequency. Energy dissipation factors were largest for waves with small orbital amplitudes and at low wave Reynolds numbers. An empirical model, commonly applied to predict friction factors by rough beds, proved applicable to the Posidonia oceanica bed. However at the lowest Reynolds numbers, under irregular waves, the data deviated significantly from the model. In addition, the wave-induced flow dissipation in the lower canopy increased with increasing wave orbital amplitude and increasing density of the mimics. The analysis of the wave-induced flow spectra confirm this trend: the reduction of flow was greatest at the longer period component of the spectra. Finally, we discuss the implications of these findings for sediment dynamics and the role of Posidonia oceanica beds in protecting the shore from erosion
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