1,721,009 research outputs found
Process for the manufacturing of glucagon.”
No full textThe present invention provides an improved process for the preparation of glucagon, comprising the coupling of an N-terminal tetramer fragment with a C-terminal peptide, comprising at least one pseudoproline. The process is very efficient in avoiding aggregation and obtaining the desired product in high yield and purity
Improved process for the preparation of high purity glucagon
No full textNew process for the synthesis of glucago
Epoxidation of 17-oxo-15,16-methylene steroids with sulfoxonium ylides
No full textA process was disclosed for the epoxidn. of 17-oxo-15,16-methylene steroids, such as I [R3a = R4 = R7 = H, R3b = OH, R5R6 = bond; R3aR3b = O, R4R5 = bond, R6R7 = CH2; R3a = R4 = H, R3b = R5 = OH, R6R7 = CH2], and comprised the use of sulfoxonium ylides, in particular trimethylsulfoxonium iodide. The process allows the prepn. of 17-spiro epoxides with good yields which can be easily transformed into therapeutically useful 17-spironolactone-steroids, such as drospirenone (II)
Epoxidation of 17-oxo-15,16-methylene steroids with sulfoxonium ylides
No full textA process was disclosed for the epoxidn. of 17-oxo-15,16-methylene steroids, such as I [R3a = R4 = R7 = H, R3b = OH, R5R6 = bond; R3aR3b = O, R4R5 = bond, R6R7 = CH2; R3a = R4 = H, R3b = R5 = OH, R6R7 = CH2], and comprised the use of sulfoxonium ylides, in particular trimethylsulfoxonium iodide. The process allows the prepn. of 17-spiro epoxides with good yields which can be easily transformed into therapeutically useful 17-spironolactone-steroids, such as drospirenone (II)
Development of freeze-drying cycle for a peptide-based drug in trays
Full text linkFreeze-drying of a peptide solution is here presented, from the early identification of critical product temperature to the design of an appropriate cycle. Synergy between experimental characterisation techniques, mathematical modelling and process simulation has been implemented to develop a robust lyophilisation process and guarantee the removal of solvents impurities. Process has been carried out in bulk using a tray equipped with an anisotropic membrane, enabling unidirectional vapour flow
Understanding glucagon aggregation: in silico insights and experimental validation
Full text linkPeptide aggregation poses a significant challenge in biopharmaceutical development and neurodegenerative diseases. This study combines computational simulations and experimental validation to uncover the underlying mechanisms and countermeasures for the aggregation of glucagon, a peptide with a high tendency to aggregate. In silico simulations demonstrate that lactose and 2-hydroxypropyl-beta-cyclodextrin (2-HP beta CD) influence glucagon aggregation differently: lactose stabilizes glucagon by increasing the alpha-helical content, while 2-HP beta CD disrupts protein-protein interactions. According to the simulations, 2-HP beta CD is particularly effective at preserving the monomeric form of glucagon. Experimental validation with microfluidic modulation spectroscopy (MMS) confirms these findings, showing that glucagon in the presence of 2-HP beta CD remains structurally stable, supporting the antiaggregation effect of this excipient. This research provides essential insights into glucagon aggregation obtained through a new powerful tool for monitoring the critical properties of peptide aggregation, suggesting new strategies for addressing this challenge in therapeutic peptide development
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
- …
