23,995 research outputs found
Molecular and Pharmacological Studies of Alpha1-AR Subtypes and their Signaling Pathways
No full textStudies in part 1 of this dissertation were intended to develop selective inhibitors of (aiBAR and ajo-AR, respectively) subtype expression using antisense oligomer approaches. Using the nucleotide sequence of the (Xib-AR and ccid-AR subtypes, antisense oligomers were designed and tested for their effectiveness at inhibiting cti-AR subtype expression in vitro. For these studies two cell culture models were used: 1) DDTi-MF2 cells expressing aiB-AR’s were used to test the potency and maximum effects of aie-AR antisense oligomers and 2) transfected HEK293 cells expressing aio-AR’s were used to test a^-AR antisense oligomers. Separate oligomers containing mismatches and/or scrambled sequences relative to the antisense constructs served as controls and were always included in parallel experiments to test for potential nonspecific effects by the antisense oligomers. Cells incubated with media alone served as additional controls. The results of the in vitro experiments in DDTi-MF2 cells and HEK293 cells are summarized below.|DDTi-MF2 cells were incubated with an 18-mer phosphorothioate antisense oligomer designed to target the translation start-site of the aie-AR mRNA. Following incubation, I measured am-AR density, aie-AR mRNA concentration and aie-AR-mediated accumulation of total [JH]-inositol phosphates and compared the results to those of control oligomer and mediaalone treated cells. [JFI]-Prazosin saturation binding experiments indicated that the antisense oligomer reduced cxib-AR density in a time- and concentration-dependent manner in cell membranes relative to control oligomer and media-alone treated cells. Competitive RT-PCR experiments showed that antisense oligomer incubation decreased a^-AR mRNA concentration relative to media-alone and control oligomer treatments. Incubation of DDT[-MF2 cells with the antisense oligomer also inhibited a.iB-AR-stimulated accumulation of total [3H]-inositol phosphates vs. that of media-alone and control oligomer cells, whereas responses to histamine stimulation were unaffected. I found no differences in a^-AR density, am-AR mRNA concentration or aie-AR-mediated accumulation of total [JH]-inositol phosphates between cells incubated with Control oligomer and media-alone. Finally, the uptake of the antisense oligomer into DDTi-MF2 cells was shown by fluorescent microscopy studies using a fluorescently labeled form of the oligomer.|Additional studies in DDTi-MF2 cells were performed to investigate whether oligomer backbone modification (phosphorothioate vs. morpholino) altered the potency of the antisense oligomer sequence characterized above. I measured ccib-AR density in [3H]-prazosin saturation binding experiments using membranes from cells treated with varied concentrations of either phosphorothioate or morpholino antisense oligomers, or their respective control oligomers or media-alone. These experiments indicated that under their optimal treatment protocols, the morpholino oligomer was 10-fold more potent than the phosphorothioate oligomer in reducing aie-AR density, but both produced an identical maximum response. Studies of ais-ARmediated accumulation of total [JH]-inositol phosphates also showed that under their optimal treatment protocols, the morpholino antisense oligomer was a 10-fold more potent inhibitor of ccib-AR function than its phosphorothioate counterpart. Again, both oligomers produced identical maximal responses. In this study, there were no differences in cxib-AR density and/or aiB-AR-mediated accumulation of total [3H]-inositol phosphates between cells incubated with control oligomers and media-alone.|Five sites of the rat aio-AR mRNA were selected with the Genetics Computer Group (GCG, Inc. Madison, WI) computer program as potential targets for oligomer hybridization. 18-mer phosphorothioate oligomers were synthesized to complement each of these sites and tested for their ability to reduce am-AR density in HEK293 cells. Using [JH]-prazosin binding experiments, I found that only the oligomer directed immediately at the translation start-site significantly reduced aio-AR density compared to media-alone treatment. Additional binding studies with this oligomer and a random-sequence control oligomer revealed that the time-course of inhibition in am-AR density was consistent with an antisense mechanism of action. Comparative RT-PCR experiments showed that the antisense oligomer reduced the steady-state aio-AR mRNA concentration without affecting the integrity of p-actin mRNA. Incubation of HEK293 cells with the ccid-AR antisense oligomer also inhibited norepinephrine-stimulated accumulation of total [JH]-inositol phosphates relative to control oligomer treated cells. Experiments were also performed in vivo to determine the effectiveness of the phosphorothioate and morpholino am-AR antisense oligomers following systemic administration in Sprague-Dawly rats. Animals treated with control oligomers and saline served as controls. Following administration, cxib-AR density in liver and kidney was measured by saturation and competition binding experiments, respectively, and the effect of antisense oligomer on spleen function was determined by contractility experiments. Saturation binding experiments with [3H]-prazosin showed that the morpholino antisense oligomer, and to a lesser extent the phosphorothioate antisense oligomer, reduced am-AR density in liver membranes of rats treated with either oligomer relative to control oligomer and saline treated animals. The morpholino oligomer was also twice as potent as the phosphorothioate oligomer. No effect on liver am-AR density was noted between control oligomer and saline-treated animals. Neither antisense oligomer was effective at inhibiting am-AR expression in kidney (as measured in competition-binding experiments with LY-757464-see Table 1 below) or am-AR function in spleen (as measured by ai-AR agonist mediated contraction).|In part 2 of this dissertation, experiments were designed to study the effects that traditional second messenger activation (increases in intracellular calcium and activation of protein kinase C) by ai-AR’s have on stimulation of mitogen activated protein (MAP) kinase pathways. Using CHO-K1 cells, we investigated the coupling of mouse ccia-AR’s to ERK1/2 and JNK pathway activation. characterization of the expressed a t-AR in CHO-K1 cells by competition binding experiments with [3H]-prazosin, 5-methyl-urapidil and BMY-7378 (see Table 1 below) revealed a receptor binding affinity typical of the cxia-AR. Experiments with the fluorescent calcium indicator fura-2 showed that the ai-AR agonist norepinephrine increased intracellular calcium levels in ciia-AR transfected CHO-K1 cells but not in untransfected CHOK1 cells. In transfected cells, norepinephrine stimulated the phosphorylation of ERK1/2 and JNK pathways in a time- and concentration-dependent fashion, as measured by western blotting. Blockade of MEK1/2 (required for ERK1/2 activation) prevented the phosphorylation of ERK1/2 by norepinephrine. Fura-2 experiments showed that the intracellular calcium chelator BAPTA blocked the norepinephrine-stimulated rise in intracellular calcium and western blotting demonstrated that the phosphorylation of ERK1/2 and JNK by norepinephrine was also abolished. Additional experiments showed that inhibition of protein kinase C abolished the norepinephrine-stimulated phosphorylation of both MAP kinases, whereas phorbol-ester-induced phosphorylation of ERK1/2 and JNK was blocked by protein kinase C inhibition.|Additional experiments in part 2 of this dissertation include studies of ERK1/2 activation by norepinephrine in freshly isolated liver slices. In these studies, I showed that norepinephrine (in the presence of a.2-AR and (3i-AR blockers) caused a time-dependent increase in the phosphorylation of the ERK1/2 MAP kinase. The stimulation of ERK1/2 MAP kinase phosphorylation by norepinephrine was partially blocked with 100 nM prazosin, whereas it was nearly abolished by L-757464 at concentrations shown to block only a^-AR’s in rat liver homogenates (Table 1).|Since MAP kinase activation has been shown to influence cellular growth and differentiation, a series of studies were designed to investigate the effect of cxia-AR stimulation on cellular morphology of transfected CHO-K1 cells. When stimulated with norepinephrine for 48 h, I found that a iA-AR transfected CHO-K1 cells changed in morphology from a stellate to a fibroblastic phenotype, characteristic of cell spreading. This morphology change was time- and concentration dependent, and did not occur in norepinephrine-stimulated untransfected CHO-K1 cells. Incubation with 100 nM prazosin prevented the norepinephrine-mediated morphology change and retarded growth of otiA-AR transfected CHO-K1 cells. In an attempt to investigate the mechanism of a iA-AR stimulated cell spreading, I incubated cells with the phorbol ester PMA (in order to down-regulate the activity of PKC) and/or PD98059 (an inhibitor of the ERK1/2 MAP kinase pathway). The effect of norepinephrine on cellular morphology was unaffected following prolonged PMA incubation, whereas incubation with PD98059 prevented the effect of norepinephrine on cellular morphology. In order to investigate the effect of a )A-AR stimulation on activation of FAK (a tyrosine kinase that has been reported to alter cell morphology upon stimulation by other GPCR’s), I immunoprecipitated FAK following norepinephrine stimulation of CHO-K1 cells. Preliminary experiments showed that norepinephrine stimulated the phosphorylation of FAK in transfected CHO-K1 cells at 5, 15 and 60 min of norepinephrine incubation relative to vehicle.ProQuest Traditional Publishing Optionxxii, 182 leave
P�gina web generada din�micamente desde bases de datos
No full textEste proyecto consiste en la implementaci�n de una aplicaci�n desarrollada utilizando la tecnolog�a Active Server Pages (ASP) desarrollada por Microsoft para poder mantener un sitio web con mayor facilidad, ya que la informaci�n se almacena en bases de datos y las p�ginas HTML son generadas de forma din�mica. Se aplic� a la p�gina principal del Centro Universitario del Sur de la Universidad de Guadalajara. Para su desarrollo se utiliz� el modelo de Construcci�n de Prototipos que permite generar una versi�n preliminar y sobre ella realizar las adecuaciones y correcciones de acuerdo a las caracter�sticas deseadas para el producto final. Como beneficio se obtuvo el ahorro de tiempo en la publicaci�n de la informaci�n anteriormente descrita, ya que el responsable o generador de la informaci�n es tambi�n el responsable de su publicaci�n en la p�gina web, adem�s de que ofrece la facilidad de mantenimiento de la informaci�n generada y sus hist�ricos
Ittijahat Abi Dhu'ayb Al-Hudhali fi Qasidatihi Ar-Ritsa’
No full textThis article aims to know Ittijahat of Abu Dhu’ayb Al-Hudhali in his poem of Ritsa’. Ittijahat in this study is a description of the poet's tendency to divide Ar-Ritsa', namely Nadb and Ta'ziyah. Ar-Ritsa 'is one of the various forms of Arabic poetry. To analyze the discussion problem, the author uses descriptive analysis method, because this method is very appropriate to find out the picture and state of a thing by describing it in as much detail as possible based on the facts found in the data in question. The results obtained are: In the beginning of the poem, he tends to mourn (Nadb) their five children. He describes in it that he does not stop crying and has trouble sleeping until he becomes weak emaciated due to the intensity of the grief, pain and worries that dominated in his pysche. As for the middle of the poem to the end, the poet turns to the consolation (Ta’ziyah) of his children’s death, as he reflects on the reality of life and death, the poet declares that death is inevitable for every human being that he must accept it and let it go, which the poet portrayed in the exciting narrative style. The story is divided into three stories: the zebra, the bull of the beast, and the two knights.</jats:p
Program “I’dad Lughowi” dalam Upaya Membekali Kemampuan Bahasa Arab Mahasiswa STIBA Ar-Raayah, Sukabumi
No full textNowdays, Arabic already has its own charm. Apart from the Al-Quran and Hadith as sources of Islamic Shari'a which are imported from the original source in Arabic, the emergence of scientific disciplines linked to Shari'a adds to the public's attraction and interest in studying Shari'a directly from its Arabic sources. Arabic language learning is starting to emerge with various strategies and methods applied. Some are successful and others need review. In this article, the author wants to examine Arabic language learning in the STIBA Ar-Raayah i'dad lughowi program which is considered successful because it has produced many regional, national and even international achievements. This article aims to examine the background of students in the STIBA Ar-Raayah i'dad lughowi program, the teaching and learning activities of the i'dad lughowi program at STIBA Ar-Raayah and the examination system implemented in the i'dad lughowi program. This article is a case study at STIBA Ar-Raayah using a descriptive qualitative approach. The results found in this research are (1) the background of students in the STIBA Ar-Raayah i'dad lughowi program who come from various regions and schools, (2) learning using the Arabiyyah book Baina Yadaik by maximizing the direct method and activities outside the classroom, (4) a unique exam system with Al-Qur'an exams, subject exams, syahri exams, nihai exams, as well as a rosib system for students who do not reach the minimum completion criteria. The results and conclusions of this research can be used as a reference for Arabic language educational institutions to make them more effective in the future
The Poetry Anthology Ar-Robi fii qalbi by Sofi Ghoniyah: A Sociology of Author Perspective
No full textThis study aims to determine the sociology of the author in the poetry anthology Ar-Robi fii Qalbi by Sofi Ghoniyah based on the perspective of Rene Wellek and Austin Warren. This research focuses on four aspects, namely (1) the social background of the author in the anthology of poetry Ar-Robi fii Qalbi by Sofi Ghoniyah based on the perspective of Rene Wellek and Austin Warren; (2) the author\u27s social integration in the poetry anthology Ar-Robi fii Qalbi by Sofi Ghoniyah based on the perspective of Rene Wellek and Austin Warren; (3) author\u27s economic sources in the poetry anthology Ar-Robi fii Qalbi by Sofi Ghoniyah based on the perspective of Rene Wellek and Austin Warren; (4) professionalism in the authorship of Sofi Ghoniyah\u27s anthology of poetry Ar-Robi fii Qalbi based on the perspective of Rene Wellek and Austin Warren. This type of research is qualitative research. The data source of this research uses an anthology of poetry entitled "Ar-robi fii qalbi" by Sofi Ghoniyah. While the secondary data sources of this research were obtained from reading books or journals discussing the sociological theory of the authors Rene Wellek and Austin Warren. Data collection techniques in this study used interview techniques and note-taking techniques. The data validation technique used in this study is to increase persistence, triangulate data, and discuss with colleagues and experts. Meanwhile, for the data analysis technique, this research uses the Miles and Huberman method, namely data reduction, data presentation, and drawing conclusions. The researchers produced several data, namely (1) the author of the poetry anthology Ar-Robi fii qalbi is an educated author and has close interactions with her environment. (2) The author of the poetry anthology Ar-Robi fii qalbi has a high degree of integration. He mingles with the general public. (3) The main economic source of the author in Ar-Robi\u27s poetry anthology is not through her work but in her educational activities. As a teacher, her work is often used as learning material for her students. (4) The author of the poetry anthology Ar-Robi fii qalbi has high professionalism in the authorship of her work
Pustakawan Dalam Perspektif Manajer Informasi: Studi Kasus Pada Mahasiswa Jurusan S1 Ilmu Perpustakaan Fakultas Adab dan Humaniora UIN Ar-Raniry
No full textThis study aims to find out Librarian in Perspective of Information Manager (Case Study on Library Science Undergraduate Student of Adab and Humanities Faculty of UIN Ar-Raniry, Banda Aceh). This research uses quality descriptive approach, an approach that emphasizes more on the deductive and inductive deduc- tion process and on the analysis of the dynamics of relationship between observed phenomena, using scientific logic. The students of Library Science were observed to be 54 people for three months. For this research the author will explain in detail about UIN Ar-Raniry Banda Aceh Main Library in the perspective of information managers for llibrary users especially Library Science students class of 2016 intake. The research results are; Librarians are not yet fully able to become information managers at UIN Ar-Raniry Main Library of Banda Aceh, due the small number of librarians for this size of a college library. Lack of librarian contribution to be- come information manager, in order to be able to fulfill requirement of information for their users, in this case undergraduate student of Library Science. Regarding the perspective that a library must be able to provide all forms of information required has not been done well, especially the information needs fot this such of users in this case Library Science students
Teacher Candidates' opinions about Augmented Reality(AR) as a supplemental educational tool
No full textThis poster was presented by Alam, I., Teacher Candidates' opinions about Augmented Reality(AR) as a supplemental educational tool. Presented at 10th Annual Graduate and Professional Student Conference, held at Memorial Union, Ames, IA, April 6 2022. Posted with permission.In recent years, augmented reality (AR) technology has been widely proposed as innovative technology. AR technology superimposes digitally simulated information on the physical environment, which can be a unique element for learning experiences. Despite the potential of AR, there has been relatively little integration of AR into the
classroom. The purpose of this pilot study is to examine the acceptance and intentional use of AR technology by teacher candidates and their opinions of AR as a supplemental educational tool. The study has utilized a phenomenological design that is comprised of System Usability Scale survey and interview questions. Based on the participants' answers, research data has been analyzed and discussed
Nonlinear stories told by cups and saucers: Smart replic as with response 3D audio
No full textIn museum exhibitions historical objects are usually shown by visual display, in a showcase with extra textual information added to it. Museum visitors can never touch the objects, let alone use them. As a result, visitors ‘scan’ the displayed objects from a distance, something that needs reconsideration in our present time where the ‘experience’ is essential. To provide a way around this situation, the so-called ‘smart replica’ was proposed in the previous issue of Ar[t] (Roozenburg, 2012): a new kind of reproduction, in the shape of a 3D print, that stretches the boundaries of the replica’s concept as an autonomous object based on a historical artefact. New methods of access and new digitization strategies based on the study of the relationship between the ‘bits’ and ‘atoms’ are being developed. We hypothesize that by using these 3D imaging techniques the value of our cultural heritage can be increased. In other words, the goal is not to make the most realistic copy of the original, but to analyse, communicate and enhance those qualities of the historical artefact that are the most meaningful to us, now. Here we present the design project of Lotte de Reus in connection with this paradigm shift. Completed as a graduation project, it presents an auditory environment to augment the artefact in an unobtrusive and non-linear way. The objects that are central in this project are seven teacups and saucers that are part of the collection of Museum Boijmans Van Beuningen. These are currently on display in a new exhibition on design and pre-industrial design. Depicted in figure 1, each of these teacup and saucer sets represents a milestone in the Dutch history of porcelain. Starting with the first import of porcelain from China in the seventeenth century by the Dutch East Indies Company; followed by the invention of Delft’s blue as an attempt to copy Chinese porcelain, and ending with the small scale production of porcelain in the Netherlands.Design EngineeringIndustrial Design Engineerin
Visualisation of PCMs through AR
No full textA master thesis what researched the visualization of phase change materials (PCMs) with augmented reality (AR) for different 3 different user groups. The user groups are researchers, maintenance and customers. The research question is "How does AR contribute to the understanding of the behavior of PCM for science, maintenance and customer groups?". With simulations in Comsol and an experiment the AR possibilities were validated. The AR gave insight in the behavior of PCM and was applicable for each group.Architecture, Urbanism and Building Sciences | Building Technolog
The poetry anthology Ar-Robi fii qalbi by Sofi Ghoniyah: a sociology of author perspective (sertifikat hak cipta)
No full textThis research focuses on four aspects, namely (1) the social background of the author in the anthology of poetry Ar-Robi fii Qalbi by Sofi Ghoniyah based on the perspective of Rene Wellek and Austin Warren; (2) the author's social integration in the poetry anthology Ar-Robi fii Qalbi by Sofi Ghoniyah based on the perspective of Rene Wellek and Austin Warren; (3) author's economic sources in the poetry anthology Ar-Robi fii Qalbi by Sofi Ghoniyah based on the perspective of Rene Wellek and Austin Warren; (4) professionalism in the authorship of Sofi Ghoniyah's anthology of poetry Ar-Robi fii Qalbi based on the perspective of Rene Wellek and Austin Warren.This type of research is qualitative research. The data source of this research uses an anthology of poetry entitled "Ar-robi fii qalbi" by Sofi Ghoniyah.While the secondary data sources of this research were obtained from reading books or journalsdiscussing the sociological theory of the authors Rene Wellek and Austin Warren. Data collection techniques in this study used interview techniques and note-taking techniques. The data validation technique used in this study is to increase persistence, triangulate data, and discuss with colleagues and experts. Meanwhile, for the data analysis technique, this research uses the Miles and Huberman method, namely data reduction,data presentation, and drawing conclusions.The researchers produced several data,namely (1) the author of the poetry anthology Ar-Robi fii qalbi is an educated author and has close interactions with herenvironment. (2) The author of the poetry anthology Ar-Robi fii qalbi has a high degree of integration. He mingles with the general public. (3) The main economic source of the author in Ar-Robi's poetry anthology is not through herwork but in her educational activities. As a teacher, herwork is often used as learning material for her students. (4) The author of the poetry anthology Ar-Robi fii qalbi has high professionalism in the authorship of her work
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