1,720,993 research outputs found

    Molecular on situ hybridization analysis of sheep and goat Bac clones identifies the transcriptional orientation pf T cell receptor gamma genes on chromosome 4 in bovids

    No full text
    The T-cell receptor gamma chain combines with the T-cell receptor delta chain to form the heterodimer gamma/delta complex. In human and mouse, gamma/delta T cells amount to less than 5% of the peripheral blood T lymphocytes (‘gamma/delta low species’), whereas in chicken and artiodactyls, gamma/delta T cells amount to about half of the peripheral blood T cells (‘gamma/delta high species’). Recent studies in bovids have revealed the existence of two paralogous loci TRG1 and TRG2. TRG1 is located on 4q31, within a region of homology with human TRG locus on chromosome 7; while TRG2, localized on 4q15–22, appears to be unique to these ruminant animals. The entire TRG2 locus is composed of three recombinational units (cassette) named TRG6, TRG2 and TRG4 according to TRGC genes. All TRG cassettes lie in the same transcriptional orientation and are closely spaced. In each ‘V, J, C’ unit, Variable and Joining genes rearrange to be found together spliced to the relevant Constant in mature transcripts. Here, we report the cytological mapping of TRG2 locus on sheep 4q22 unique chromosome band and on homologous chromosome bands of cattle, goat and river buffalo. Moreover molecular characterization of 5' boundaries of sheep TRG2 locus and dual-colour FISH results allowed us to identify the physical order of the tightly linked TRG2 and HGF loci markers along the chromosome and establish the transcriptional orientation towards the centromere of Variable (V), Joining (J) and Constant (C) TRG2 gene segments on 4q22 band

    Going Beyond Counting First Authors in Author Co-citation Analysis

    Full text link
    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

    Full text link
    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    The River Buffalo (Bubalus bubalis, 2n = 50) Cytogenetic Map: Assignment of 64 Loci by Fluorescence In Situ Hybridization and R-banding

    No full text
    Sixty-four genomic BAC-clones mapping five type I (ADCYAP1, HRH1, IL3, RBP3B and SRY) and 59 type II loci, previously FISH-mapped to goat (63 loci) and cattle (SRY) chromosomes, were fluorescence in situ mapped to river buffalo R-banded chromosomes, noticeably extending the physical map of this species. All mapped loci from 26 bovine syntenic groups were located on homeologous chromosomes and chromosome regions of river buffalo and goat (cattle) chromosomes, confirming the high degree of chromosome homeologies among bovids. Furthermore, an improved cytogenetic map of the river buffalo with 293 loci from all 31 bovine syntenic groups is reported

    Appropriate Similarity Measures for Author Cocitation Analysis

    Full text link
    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Clinical, histological and cytogenetic observations on nine intersex dogs

    No full text
    International audienc
    corecore