1,720,962 research outputs found
ANTIBIOTIC RESISTANCE IN STAPHYLOCOCCUS SPP STRAINS ISOLATED FROM GOATS’ SUBCLINICAL MASTITIS
No full textAflatoxin M1 in bulk-tank raw milk produced in a low risk area
No full textThe present study was designed to determine the levels of aflatoxin M1 (AFM1) and its seasonal variation in bulk tank cow milk collected from dairy farms located on the Italian island of Sardinia. Cow bulk tank milk samples (No 356) were collected from 178 farms twice a year, during the winter and summer seasons. All samples were analysed for AFM1 with a commercial ELISA kit. Confirmation of AFM1 positive samples (>10 ng L-1) was accomplished by using high-performance liquid chromatography (HPLC) with a prior clean-up step using immunoaffinity columns. AFM1 was found at levels ≥5 ng L-1 in 112 samples (31.5%) but only one exceeded the maximum EC level (50 ng L-1). There was no seasonal influence on the aflatoxin content of the milk samples analysed
CARATTERIZZAZIONE GENOTIPICA E STUDIO DI CLONALITÀ DI CEPPI DI S. AUREUS ISOLATI DA LATTE DI CAPRA ALLA PRODUZIONE
No full textThirty S. aureus strains isolated from goat’s bulk milk were analysed by
multiplex PCR for the following genes: gyrA (gyrase); agr I-IV alleles (accessory gene
regulator); sea-e, seh, sek, sel, sem, seo (enterotoxins genes); tst (TSST-1); eta, etb, etd
(exfoliatins); lukF-PV and lukS-PV (Panton-Valentine leukocidin); lukE and lukD (lukE-lukD
leukocidin); lukM (lukM leukocidin); hlg, hlgv and hlb (γ, γ variant and ß hemolysines); edin
(epidermal cell differentiation inhibitor); mecA (methicillin resistance determinant). All strains
were also analysed by PFGE and MLST. Genes agr III, sec, sel, tst, LukE-lukD, lukM, hlgv and
hlb were found in majority strains, while agr IV, seb, seh, mecA, eta, etb, etd, lukF-PV and
lukS-PV were not found. PFGE and MLST subdivided the strains respectively in 14 pulsotypes,
7 groups of similarity and 7 classes
DEFINIZIONE DELLA SHELF-LIFE DI SPARUS AURATA CONSERVATA IN GHIACCIO PROVENIENTE DA ALLEVAMENTI OFF-SHORE DELLA SARDEGNA
No full textOccurrence of Aflatoxin M1 in tank bulk goat milk and ripened goat cheese
No full textThis paper reports the results of a two year survey on AFM1 contamination in goat milk and hard goat cheese produced in Sardinia
(Italy). The AFM1 content in 208 bulk-tank goat milk samples was measured, using an ELISA commercial kit. The samples came from 45
extensive and 7 intensive farms. During each lactation period, four samples were collected. The AFM1 content was also studied in 41 hard
goat cheese samples from 12 cheese factories. AFM1 was detected in 36 bulk-tank goat milk samples (17.3%) at concentrations of between
5 and 40 ng/l, while in 172, it was not detectable (<5 ng/l). The incidence of contaminated samples was higher in intensive (71.4%) than in
extensive ones (11.2%). AFM1 was also detected in 4 (9.8%) out of 41 samples of ripened goat cheese at levels of between 79.5 ng/kg and
389 ng/kg. These contaminated samples were obtained from the same cheese factory where the milk from the intensive farms was used
CARATTERI ORGANOLETTICI E DI COMPOSIZIONE DELLE CARNI DI BOVINI DI RAZZE RUSTICHE DELLA SARDEGNA
No full textCarcass weight, pH decrease, chilling time, composition and colour (CIELab) were evaluated in 49 bovine carcasses of Sardinian rustic breeds. The results showed: a correct pH decrease (pH u 5,52 ± 0,11) and cooling (T 3,5 ± 1,9), a good level of meat redness value (a*) in the M. longissimus dorsi at 24 h and a good composition. The L*
value was affected by the pH value (p<.01)
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
“Caratteri organolettici e di composizione delle carni bovine di razze rustiche della Sardegna”
No full textListeria monocytogenes contamination sources in sheep cheese processing plants and strains virulence genes typing
No full textA survey on Listeria monocytogenes (Lm) contamination patterns was carried out in 10 sheep cheese processing plants, located in Sardinia (Italy). The samples were collected from the processing line during production. The samples were taken in cheese and ricotta processing area from food contact surfaces (raw milk filters, moulds, tables, ripening shelves and cheese and ricotta washing machine) and non food contact surfaces (floor drains or floors/walls). Pooled samples from rind surfaces of hard cheese and ripened ricotta were also taken.
Lm strains were isolated from 48 samples (18.8%) taken from 9 cheese factories (90%) and other Listeria spp strains were isolated from 39 samples (15.3%) from 10 cheese factories (100%). Lm was detected in raw milk filters (16.7%), cheese rinds (5.4%), ripened ricotta surfaces (18.2%), cheese moulds (20%), tables and shelves (9.8%), cheese washing machines (60%), ricotta washing machines (20%), drains or walls/floors surfaces (19.4%). Lm strains analysed for serotype with traditional and molecular method belonged to groups 1/2a (62,8%),
4/b (19,8%), 1/2b (14,0%) and 1/2c (3,5%). All Lm strains analysed for virulence genes prfa,
clp, inlA, inlB, inlC, hly, lisR, acta A , acta A
were found as positive
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