101,971 research outputs found
High concentrations of prostate-specific antigen in urine of women receiving oral contraceptives
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The Gravettian mandibole from Grotta Paglicci (Apulia, Italy): a geometric morphometric study
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Metallo-β-lactamase producers in environmental microbiota: New molecular class B enzyme in Janthinobacterium lividum
No full textEleven environmental samples from different sources were screened for the presence of metallo-beta-lactamase-producing bacteria by using a selective enrichment medium containing a carbapenem antibiotic and subsequently testing each isolate for production of EDTA-inhibitable carbapenemase activity. A total of 15 metallo-beta-lactamase-producing isolates, including 10 Stenotrophomonas maltophilia isolates, 3 Chryseobacterium spp., one Aeromonas hydrophila isolate, and one Janthinobacterium lividum isolate (a species in which production of metallo-beta-lactamase activity was not previously reported), were obtained from 8 samples. In the J. lividum isolate, named JAC1, production of metallo-beta-lactamase activity was elicited upon exposure to beta-lactams. Screening of a JAC1 genomic library for clones showing a reduced imipenem susceptibility led to the isolation of a metallo-beta-lactamase determinant encoding a new member (named THIN-B) of the highly divergent subclass B3 lineage of metallo-beta-lactamases. THIN-B is most closely related (35.6% identical residues) to the L1 enzyme of S. maltophilia and more distantly related to the FEZ-1 enzyme of Legionella gormanii (27.8% identity) and to the GOB-1 enzyme of Chryseobacterium meningosepticum (24.2% identity). Sequences related to bla(THIN-B), and inducible production of metallo-beta-lactamase activity, were also detected in the J. lividum type strain DSM1522. Expression of the bla(THIN-B) gene in Escherichia coli resulted in decreased susceptibility to several beta-lactams, including penicillins, cephalosporins (including cephamycins and oxyimino cephalosporins), and carbapenems, revealing a broad substrate specificity of the enzyme. The results of this study indicated that metallo-beta-lactamase-producing bacteria are widespread in the environment and identified a new molecular class B enzyme in the environmental species J. lividum
Fever with spontaneous gingival bleeding: A diagnostic challenge
No full textA previously healthy 4-year-old girl was admitted to our institution
with fever and painless spontaneous gingival bleeding, persisting over a
week, without evident lesions or trauma. She was born in Italy and had
always lived in Palermo. She had never travelled internationally. Lab oratory testing showed a negative swab test for Sars-CoV2 and a tri linear pancytopenia with neutropenia (0.6 × 103/mm3), lymphopenia
(1.17 × 103/mm3), thrombocytopenia (115 × 103/mm3) and anemia
(Hb 7.1 g/dL, RBC 3.48 × 103/mm3, Hct 21.2%, RDW-CV 23.4%, RDW SD 51,6 fL). Other laboratory findings showed hyponatremia (130
mmol/dL), hypocalcemia (8.7 mmol/dL), hypoalbuminemia (3.5 g/dL),
increase of LDH (267 IU/L), C-reactive protein (1.64 mg/dL) and alka line phosphatase levels (190 IU/L). On physical examination she
appeared in pain, pale, with hyperemic pharynx without plaque or pu rulent secretion and hypertrophic bleeding gingiva (see Fig. 1). There
was notable cervical lymphadenopathy, hepatomegaly (1 cm below the
costal margin), and splenomegaly (3 cm below the costal margin). An
abdominal ultrasound showed an enlarged spleen (15.6 × 6.6 cm) and
ascites.
Additional history revealed the diagnosis of visceral leishmaniasis,
treated with six doses of Amphotericin B liposomal (administered for
first 5 days plus one dose on the 10th day) seven months earlier. A
subsequent leishmania polymerase chain reaction (PCR) assay on blood
and gingival brush confirmed the diagnosis of a leishmaniasis relapse. A
comprehensive immunological evaluation including an HIV test
excluded any primary or acquired immunodeficiency disease. Treatment
with amphotericin B was administered over 10 days, leading to a full
recovery. Visceral leishmaniasis caused by Leishmania infantum is
endemic in the Mediterranean region. Mucocutaneus lesions are
extremely rare with this species and usually affect immunosuppressed
patients. In children leishmaniasis relapse with atypical mucosal
involvement represent a rarity. Clinical follow up and periodic PCR tests
(performed at 3-6-9-12 months after treatment) should be considered for
early recognition of a relapse
New Evidence of Vegetable diet in the Gravettia of Grotta Paglicci (Rignano Garganico, Puglia, Southern Italy)
No full textDes restes humains dans le site paléolithique moyen ancien de Payre (Ardèche) : dents et pariétal
No full textABSTRACT Payre is a Middle Palaeolithic site which has been excavated since 1990. Radiometric dates give an age between isotopic stages 7 to 5. In 1994, 2 teeth were discovered in the deepest level G. In 1995, 2 more teeth were discovered in the same level. Summer 1996 gave other human bones : 2 teeth in G, 1 tooth in D and especially a piece of left parietal in level G. These remains complement those already known in the region : the earlier human theeth of Orgnac 3 and other, more recent, ones from Soyons. They provide elements for studying the population of the Rhône middle valley during the Middle Pleistocene period.RÉSUMÉ Le site Paléolithique moyen ancien de Payre est fouillé depuis 1990. Les datations radiométriques donnent un âge compris entre les stades isotopiques 7 à 5. En 1994 ont été mises au jour deux dents humaines dans la couche la plus profonde G. En 1995, deux nouvelles dents, toujours dans la même couche, sont découvertes. La campagne 1996 a livré encore d'autres restes humains : deux dents dans la couche G, une dent dans la couche D et surtout un fragment de pariétal gauche dans la couche G. Ces restes s'ajoutent à ceux déjà connus dans la région, plus vieux à Orgnac 3, plus récents à Soyons et permettent d'appréhender le peuplement de la moyenne vallée du Rhône durant le Pleistocene moyen.Moncel Marie-Hélène, Condemi Silvana. Des restes humains dans le site paléolithique moyen ancien de Payre (Ardèche) : dents et pariétal. In: Bulletin de la Société préhistorique française, tome 94, n°2, 1997. pp. 168-171
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