135 research outputs found

    Investigation of effects of fluorosis on some minerals and hormones in sheep

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    This study was carried out to determine the effects of fluorosis on plasma fluor (F), serum calcium (Ca), phosphorus (P), magnesium (Mg), calcitonin (CT), intact parathyroid hormone (iPTH) and vitamin D-3 (Vit D-3) in sheep. A total of 75 Morkaraman sheep, 50 with chronic fluorosis (Fluorotic group) from Tendurek Mountain region (altitude about 2000 m) and 25 without any fluorosis (Control group) from Van (altitude 1700m) were used, aged 3-4 years. Data collected 50 sheeps with chronic fluorosis in the in East Anatolia, Van-Agri, Turkey, were compared with data from 25 without any fluorosis sheeps from the Van region. Plasma F levels were measured with spesific fluride electrode, serum Ca, P and Mg levels were photometrically determined; serum CT and iPTH levels were determined using chemiluminescent immunometric assay, serum Vit D-3 level were analysed with HPLC method. Serum P and Vit D-3 levels were lower in fluorotic group than in control group (p<0.001), while serum CT (p<0.01) and iPTH (p<0.001) levels were higher. The differences for Ca and Mg values were found statistically nonsignificant. The results obtained were after a long term exposure of sheep to high amounts of fluorine and supports occurrence of osteomalatic conditions. In conclusion, clean water should be given to animal and people who live in this region because of irreversible pathological situation occurs after fluorosis develops. Otherwise, Ca, P, Mg and Vit D-3 supplementations has to be given at proper doses to prevent negative effects of fluoride on teeth and bones

    Leptin Levels and Lipids Profile Determination in Different Sheep Breeds

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    Leptin hormone is produced by fat cells and is believed to coordinate the control of body weight. This study was conducted to determine the levels of leptin, triglyceride, cholesterol, VLDL, LDL, HDL and glucose in serum apart from the body weight of animals, and to find out the correlations between them. Four breeds of sheep (Karakul; semi-fat tailed breed, Morkaraman and Norduz; fat tailed breeds, Tahirova; thin tailed breed) were used as research material under the same feeding conditions. Fifteen sheep of each group -for a total of 60- were selected as research material. ELISA tests were used to determine serum leptin levels; auto analyzer was used for the estimation of triglyceride, cholesterol, VLDL, LDL, HDL and glucose levels. Body weights were measured by a weighing scale. Leptin levels, along with triglyceride, cholesterol, VLDL, LDL, HDL levels and body weight, were higher in Tahirova sheep breed, and the difference was found statistically significant (P<0.05). There was no statistically significant difference between the HDL levels of sheep breeds. Glucose levels were high in Karakul breed and statistically significant difference was determined between Karakul, Norduz and Tahirova breeds (P<0.05). In conclusion, thin tailed sheep breeds were found to have a high leptin level, which shows that leptin levels are independent of the tail fat ratios. Leptin has been studied in detail in human medicine, but researches on animals were scarce. Apparently, further studies are needed to elucidate the factors of production of leptin in animals. (C) 2015 PVJ. All rights reserve
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