1,720,958 research outputs found
Epidemiological study on post-mortem findings in slaughter animals in Sardinia: prevalence and impact on the human health
No full textMultidrug resistant and Methicillin Resistant Staphylococcus aureus (MRSA) isolated from raw sheep’s milk
No full textMultidrug resistant (MDR) and Methicillin Resistant Staphylococcus aureus (MRSA) contamination in milk and dairy products can origin from animals, farm environment, from human in contact with animals or food handlers. Therefore, milk and dairy products could represent a potential source of antibiotic resistant S. aureus strains that could reach human through the food chain. Most of the reports on the occurrence and characterization of MDR S. aureus and MRSA refer to dairy cows, while little information is cu ently available on st ains isolated f om s eep’s milk. T e aim of t e p esent study was to evaluate the presence of MDR S. aureus and MRSA harboring mecA and mecC genes in aw s eep’s milk. enotypic esistance to antibiotics and t e p esence of t e genetic determinants were also investigated. Bulk tank milk samples and milking machines filters were collected from 17 Sardinian dairy sheep farms. In addition, 3 filters from one cheese-making plant collecting milk from the investigated farms were sampled. The detection of Coagulase Positive Staphylococci was performed according to ISO 6888-
1:1999 and the potential presence of MRSA assessed using ChromID MRSA Smart agar plates. Isolates were submitted to PCR for species identification. Minimum Inhibitory Concentration (MIC) for Ampicillin (AM), Cephalothin (CEF), Cefoxitin (FOX), Erythromycin (E), Oxacillin (OX), Penicillin (P), Tetracycline (TE) and Vancomycin (VA) was determined using broth microdilution method (CLSI M07,M100, 2015). The detection of the genes mecA, mecC, blaZ, ermA-B-C, vanA, tetK-M-S-W encoding antibiotic resistance was performed as previously described (Spanu et al., 2014). In this study, 118
S. aureus strains were collected, 65 strains from 17 positive milk filters, 38 from 7 bulk tank milk samples and 15 from cheese-making plant filters. Twelve strains (10.2%) were resistant at least to one of the β-lactam antibiotics tested and 6 isolates showed multiple resistance against AM, FOX, OX and/or P. Among these, 3 strains were identified as MRSA with MIC values of 16-32 μg/mL for OX and 64 μg/mL for FOX. Interestingly, 2 out of 3 MRSA were also resistant to E (8 μg/mL), despite only 1 strain carried blaZ, mecA, mecC and ermB-C genes. All the isolates were susceptible to CEF and VA and did not carry the correspondent resistant genes. On the other hand, although resistance to TE was not found, 15 and 7 S. aureus strains carried tetM and tetK genes, respectively. The results of the present study suggest the emergence of MDR S. aureus also in small ruminants dairy chain which pose a potential public health hazard for the spreading of MRSA strains
Microbial safety of Salsiccia Sarda, a typical Italian semi-dry naturally fermented sausage
No full textTipizzazione mediante riboprinting di ceppi di Listeria monocytogenes isolati da alimenti ready to eat
No full text
Microbiological and chemical-physical parameters during the processing of a typical dry ham from sarda sheep breed
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
- …
