314 research outputs found
PL10 DEAD-Box Protein is Expressed during Germ Cell Differentiation in the Reptile Podarcis sicula (Family Lacertidae)
No full textAmong genes involved in the regulation of germ cell differentiation, those of DDX4/Vasa and the Ded1/DDX3 subfamilies encode for DEAD-box ATP-dependent RNA helicases, proteins involved in many mechanisms related to RNA processing. For the first time in reptiles, using specific antibodies at confocal microscopy, we analysed the localization pattern of a Ded1/DDX3 subfamily member in testis and ovary of Podarcis sicula (Ps-PL10) during the reproductive cycle. In testis, Ps-PL10 is expressed in the cytoplasm of spermatocytes and it is not detected in spermatogonia. Differently from Ps-VASA, in round spermatids, Ps-PL10 is not segregated in the chromatoid body but it accumulates in the cytoplasm of residual bodies, and mature spermatozoa are unstained. These observations suggest that in males, Ps-PL10 (1) is involved in spermatogenesis and (2) is then eliminated with residual bodies. In the ovary, Ps-PL10 is present with granules in the cytoplasm of early meiotic cells of the germinal bed (GB), while it is not present in oogonia and somatic cells of the GB stroma. In follicular cells of ovarian follicles, Ps-PL10 expression starts after their fusion with the oocyte. Numerous Ps-PL10 spots are visible in pyriform (nurse-like) cells concomitantly with the protein accumulation in the cytoplasm of differentiating oocyte. In pyriform cells, Ps-PL10 spots are present in the cytoplasm and nuclei, as observed for Ps-VASA, and in the nucleoli, suggesting for Ps-PL10 a role in rRNA processing and in the transport of molecules from the nucleus to cytoplasm and from nurse cells to the oocyte
sd920/FIJI-macros-for-IHC-and-SHG-analysis: Batch Split Channels (3 channels)
No full textThis macro allows to split channels for Z-stack .tiff files and save them in a new folder in batch mode.
Author: Samuele Di Carmine, [email protected]
Version 1.0
July 13, 2021
//License: BSD3
Copyright 2021 Samuele Di Carmine, Imperial College Londo
Level of mitochondrial heteroplasmy in a natural and stable heteroplasmic system
No full textMitochondrial heteroplasmy is the presence of more than one type of mitochondrial genome
(mtDNA) within an individual, and in most of the reported cases it seems to be an unfavourable
condition. For example, heteroplasmy in humans increases the risk of common age-related disorders
as neurodegenerative diseases. The only evolutionarily stable and natural heteroplasmic system in
Metazoa is the Doubly Uniparental inheritance (DUI), typical of some bivalves, in which two
mitochondrial lineages are present: one transmitted through eggs (F-type) and the other through
sperm (M-type). While females are homoplasmic for the F-type, males have M-type-homoplasmic
gametes, but heteroplasmic soma. So far, no study has investigated mitochondrial heteroplasmy at the
protein level, and no analysis has been performed to clarify if it is present at tissue, cell, or organelle
level. We characterized the expression of three mitochondrially-encoded proteins (ND5, CYTB,
COX3) in the DUI species Ruditapes philippinarum. Specific antibodies were produced to
discriminate, with immunolocalization, between the F and M form of the same protein (variants
highly divergent in DUI species) in germ line and somatic tissues of females and males in different
developmental stages. Unexpectedly, M-type antibodies labelled mitochondria in female primordial
stem cells (PriSCs), undifferentiated germ cells, and early oocytes, while mature eggs and female
somatic cells expressed only the F-type. M- and F-type expression in male somatic tissues showed
mitochondrial heteroplasmy at the cell level. Interestingly, F-labelled mitochondria were detected in
male PriSCs and early germ cells. We hypothesize that PriSCs and undifferentiated germ cells carry
both types of mtDNA, but during gamete maturation one of the two genomes disappears, and only the
sexspecific mtDNA remains, maintaining the homoplasmy of the germ line. This would imply a
selective degradation of mitochondria in adulthood
Emory S. Bogardus. La distanza sociale
No full textThe volume deals with the social and historical reconstruction of the personal, environmental and cultural aspects that characterized the career path of the American sociologist Bogardus, author of the concept of social distance. The social factors that heavily invested the USA in the 19th century as lands of considerable migratory flows are analyzed. This movement can be further distinguished by referring to two migratory subperiods in which there was a succession of a so-called "first wave" up to 1880, and a "second wave" after 1880. These groups of immigrants settled consistently in the geographical area in the North (both East and West).
At the beginning of the twentieth century, a "third wave" followed, during which immigrants came mainly from Asian countries such as Japan, Korea, China and India and settled substantially on the west coast, particularly in the Californian region.
After a brief biographical note by the North American sociologist, the scientific interest of American sociology towards these profound changes, in particular of the Chicago School, will also be analyzed; the scientific debate and the main scholars who have contributed to the conceptualization first and then operationalization of the concept of social distance with the Social distance Scale and its relevance in the panorama of social sciences
Il ruolo dell’interpretazione nell’adeguamento del diritto tributario all’innovazione tecnologica: l’Iva sui servizi “ricreativi” prestati mediante Internet
No full textStarting from the analysis of the Advocate General’s conclusions, the author aims to evaluate the opportunity to use interpretation as a tool to adapt the positive rules to the economic reality of reference, in absence of new law reforms. After the examination of the peculiarities of the case, the article intends to assess if the conclusions’ findings could by applied even in other sectors of the taxation system, and if they may resist in front of the Court
Ritualità e forme di culto funerario tra VI e V sec. a.C.
No full textIn the Etruscan-Samnite settlement of Pontecagnano, the south necropolis, which formed in the sixth and fifth century, consists of distinct funerary areas reserved for élite groups. Recent excavations have allowed a complete investigation of one of these areas. The author analyzes its spatial organization and demographic composition, as well as its funerary behavior and how it changed between the beginning of the sixth and the middle of the fifth century. For instance, as regards cremation, he reconstructs the different phases of the ritual, which shows a strong Greek influence.
Complex rites correlated to specific eschatological beliefs are observable in some funerary contexts. They include the manipulation of bone remains or the use of pots with pierced bottoms, or placed
upside-down. Moreover, archaeological excavations have unearthed structures for the canalization and collecting of water, as well as areas in cemeteries set aside for ceremonies such as animal sacrifice and food consumption
FEACE, «I REMATORI DELLE MUSE» E UN CASO DI AUTO-METAPOIESIS SIMPOSIALE (DIONISIO CALCO, FR. 3 E FR. 5 GENT.-PR. = FR. 4 E FR. 5 WEST)
No full textThe ‘you’ addressed at the beginning of the fr. 3 Gent.-Pr. (= 4 West) by Dionysius Chalcus may be recog- nized as the same Phaeax as the one mentioned in v. 5 that is present at the sym- posium. He is the well-known Athenian orator and politician and a contemporary of Alcibiades’s. Phaeax will start (note πέμπει in v. 5) the sympotic chain of songs in praise of a distant friend. The sequence of songs is imagined as the work of the «Muses’ rowers». The rowing metaphor, that is used in a sympotic context once again, is present in fr. 5 Gent.-Pr. (= 5 West), where it describes the dense sequence of drinking cups. The two fragments could represent a case of auto-metapoiesis by the author, that is to say, the retake and variation made by a poet in relation to one of his previous poems that is already known to the symposium audience
A novel dataset on legal traditions, their determinants, and their economic role in 155 transplants
No full textAbstractThe law and the economy are deeply influenced by the legal tradition or origin, which is the bundle of institutions shaping lawmaking and dispute adjudication. The two principal legal traditions, common law and civil law, have been transplanted through colonization and occupation to the vast majority of the jurisdictions in the world by a group of European countries. Here, I illustrate a novel dataset recording the lawmaking institution employed by 155 of these jurisdictions at independence and in 2000 and four discretion-curbing adjudication institutions adopted by 99 of these “transplants” at the same two points in time. Contrary to the “legal origins” scholars׳ assumption, 25 transplants changed the transplanted lawmaking institution and 95 modified at least one of the transplanted lawmaking and adjudication rules. In “Endogenous Legal Traditions” (Guerriero, 2016a) [12], I document that these reforms are consistent with a model of the design of legal institutions by societies heterogeneous in their endowment of both the extent of cultural heterogeneity and the quality of the political process. In “Endogenous Legal Traditions and Economic Outcomes” (Guerriero, 2016b) [13] moreover, I show the relevance of considering legal evolution and the endogeneity between legal traditions and economics outcomes. The data illustrated here also include the proxies for the determinants of legal evolution I use in “Endogenous Legal Traditions” (Guerriero, 2016a) [12] and the novel measure of economic outcomes I employ in “Endogenous Legal Traditions and Economic Outcomes” (Guerriero, 2016b) [13]
«Autorità senza autore» nella Grecia antica: il caso dello scettro
No full textStarting from an important work by Carlo Severi dedicated to forms of "authority without an author" in modern societies that do not have a written tradition, this article analyzes the cultural mechanisms by means of which the authority of the sceptre is constructed in a prevalently oral world like that described in the Homeric poems. Through comparison with ritually subjectivized artifacts like the nkisi, to which a mainly judicial function is attributed among the Zinganga of Congo, we demonstrate that the sceptre of Agamemnon, venerated in the Boeotian city of Chaeronea as the chief deity of the polis, has a specific "agency", presenting itself – in Alfred Gell's terms – as "the visible knot which ties together an invisible skein of relations, fanning out into social space and social time"
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