1,954,334 research outputs found
Long-Range Chromosomal Mapping of the Carcinoembryonic Antigen (CEA) Gene Family Cluster
No full textA long-range physical map of the carcinoembryonic antigen (CEA) gene family cluster, which is located on the long arm of chromosome 19, has been constructed. This was achieved by hybridization analysis of large DNA fragments separated by pulse-field gel electrophoresis and of DNA from human/rodent somatic cell hybrids, as well as the assembly of ordered sets of cosmids for this gene region into contigs. The different approaches yielded very similar results and indicate that the entire gene family is contained within a region located at position 19q13.1–q13.2 between the CYP2A and the D19S15/D19S8 markers. The physical linkage of nine genes belonging to the CEA subgroup and their location with respect to the pregnancy-specific glycoprotein (PSG) subgroup genes have been determined, and the latter are located closer to the telomere. From large groups of ordered cosmid clones, the identity of all known CEA subgroup genes has been confirmed either by hybridization using gene-specific probes or by DNA sequencing. These studies have identified a new member of the CEA subgroup (CGM8), which probably represents a pseudogene due to the existence of two stop codons, one in the leader and one in the N-terminal domain exons. The gene order and orientation, which were determined by hybridization with probes from the 5′ and 3′ regions of the genes, are as follows: cen/3′-CGM7-5′/3′-CGM2-5′/5′-CEA-3′/5′-NCA-3′/5′- CGM1-3′/3′-BGP-5′/3′-CGM9-5′/3′-CGM6-5′/5′-CGM8-3′/PSGcluster/qter
Réacteurs nucléaires à caloporteur gaz
No full textInternational audienceLes progrès accomplis récemment dans le domaine des matériaux et des composants à haute température remettent sur le devant de la scène les réacteurs à caloporteur gaz qui ont déjà connu un premier essor avec les réacteurs Magnox et UNGG.Dans leur version à neutrons lents, des réalisations à l'échelle industrielle sont envisageables à moyen terme. Dans leur version à neutrons rapides, plus prospective, les réacteurs à caloporteur gaz offrent des perspectives supplémentaires de valorisation énergétique des ressources en uranium naturel, dans le cadre d'un cycle du combustible minimisant les déchets ultimes et le risque de prolifération.La présente monographie, premier ouvrage de la collection après le hors-série de présentation, décrit les recherches en cours sur ce type de réacteur, en montrant les enjeux, les résultats récents obtenus par le CEA et les obstacles qu'il reste à franchir
A Polymerase-chain-reaction Assay for the Specific Identification of Transcripts Encoded by Individual Carcinoembryonic Antigen (CEA)-gene-family Members
No full textCarcinoembryonic antigen (CEA) is a tumor marker that belongs to a family of closely related molecules with variable expression patterns. We have developed sets of oligonucleotide primers for the specific amplification of transcripts from individual CEA-family members using the reverse transcriptase/ polymerase chain reaction (RT/PCR). Specific primer sets were designed for CEA, non-specific cross-reacting antigen (NCA), biliary glycoprotein (BGP), carcinoembryonic antigen gene-family members 1, 6 and 7 (CGMI, CGM6 and CGM7), and one set for all pregnancy-specific glycoprotein (PSG) transcripts. Primers were first tested for their specificity against individual cDNA clones and product-hybridization with internal, transcript-specific oligonucleotides. Total RNA from 12 brain and 63 gynecological tumors were then tested for expression of CEA-related transcripts. None were found in tumors located in the brain, including various mesenchymal and neuro-epithelial tumors. CEA and NCA transcripts were, however, present in an adenocarcinoma located in the nasal sinuses. In ovarian mucinous adenocarcinomas, we always found co-expression of CEA and NCA transcripts, and occasionally BGP mRNA. CEA-related transcripts were also found in some serous, endometrioid and clear-cell ovarian carcinomas. CEA, NCA and BGP transcripts were present in endometrial carcinomas of the uterus and cervical carcinomas, whereas uterine leiomyomas were completely negative. No transcripts were found from CGM 1, CGM6, CGM7 or from PSG genes in any of the tumors tested. The PCR data were compared with immunohistochemical investigations of ovarian tumors at the protein level using CEA (26/3/13)-, NCA-50/90 (9A6FR) and NCA-95 (80H3)-specific monoclonal antibodies
O sistema de Informação sobre a Comercialização Agrária
No full textA report produced by the CEA in 1982. Portuguese language. This copy was given to the Ruth First Papers project by Colin Darch
Fámilias Camponesas de Angónia no Processo de Socialização do Campo
No full textA report produced by the CEA in 1983. Portuguese language. This copy was given to the Ruth First Papers project by Colin Darch
O trabalhador sazonal na transformação duma economia de plantações
No full textA report produced by the CEA in 1981. Portuguese language. This copy was given to the Ruth First Papers project by Colin Darch
Problemas de transformação rural na província da Gaza
No full textA report produced by the CEA in 1979. Portuguese language. This copy was given to the Ruth First Papers project by Colin Darch
Poder Popular e Desagregação nas Aldeias Comunais do Planalto de Mueda
No full textA report produced by the CEA in 1986. Portuguese language. This copy was given to the Ruth First Papers project by Colin Darch
Circuitos de troca e transporte no desenvolvimento do campo
No full textA report produced by the CEA in 1981. Portuguese language. This copy was given to the Ruth First Papers project by Colin Darch
Organizar os Trabalhadores das Machambas Estatais: O Caso da C.A.I.A
No full textA report produced by the CEA in 1983. Portuguese language. This copy was given to the Ruth First Papers project by Colin Darch
- …
