2,061 research outputs found
Ghrelin enhances in vivo skeletal muscle but not liver AKT signaling in rats
No full textOBJECTIVE: Ghrelin administration can induce fat weight gain and hyperglycemia (potentially through ghrelin-induced hepatic glucose production), but plasma ghrelin is positively associated with whole-body insulin sensitivity (mainly reflecting muscle insulin action) being increased in lean individuals or after diet-induced weight loss and reduced in obesity or after diet-induced weight gain. To investigate potential mechanisms, we measured in vivo effects of sustained ghrelin administration at a non-orexigenic dose on skeletal muscle and liver insulin signaling at the AKT level and adipokine expression changes.
RESEARCH METHODS AND PROCEDURES: Young-adult male rats received 4-day, twice daily subcutaneous ghrelin (200 mug/injection) or saline. We measured skeletal muscle (mixed, gastrocnemius; oxidative, soleus) and liver protein levels of activated [phosphorylated (P)] and total (T) AKT and glycogen synthase kinase (GSK; reflecting AKT-dependent GSK inactivation) and epididymal adipose tissue adipokine mRNA.
RESULTS: Ghrelin increased body weight (+1.4%) and blood glucose (both p < 0.05 vs. saline) but not food intake, plasma insulin, or free fatty acids. Ghrelin, however, enhanced P/T/AKT and P/T/GSK ratios and glucose transporter-4 mRNA in soleus (p < 0.05), but not in gastrocnemius, muscle. In contrast, ghrelin reduced hepatic P/T-AKT and P/T-GSK. No alterations occurred in adiponectin, leptin, or resistin transcripts or plasma adiponectin.
DISCUSSION: Despite moderate weight gain and in the absence of insulin-free fatty acid changes, sustained ghrelin administration enhanced oxidative muscle AKT activation. Reduced liver AKT signaling could potentially contribute to concomitant blood glucose increments. These findings support ghrelin as a novel tissue-specific modulator of lean tissue AKT signaling with insulin-sensitizing effects in skeletal muscle but not in liver in vivo
High-fat diet with acyl-ghrelin treatment leads to weight gain with low inflammation, high oxidative capacity and normal triglycerides in rat muscle.
No full textObesity is associated with muscle lipid accumulation. Experimental models suggest that inflammatory cytokines, low mitochondrial oxidative capacity and paradoxically high insulin signaling activation favor this alteration. The gastric orexigenic hormone acylated ghrelin (A-Ghr) has antiinflammatory effects in vitro and it lowers muscle triglycerides while modulating mitochondrial oxidative capacity in lean rodents. We tested the hypothesis that A-Ghr treatment in high-fat feeding results in a model of weight gain characterized by low muscle inflammation and triglycerides with high muscle mitochondrial oxidative capacity. A-Ghr at a non-orexigenic dose (HFG: twice-daily 200-μg s.c.) or saline (HF) were administered for 4 days to rats fed a high-fat diet for one month. Compared to lean control (C) HF had higher body weight and plasma free fatty acids (FFA), and HFG partially prevented FFA elevation (P<0.05). HFG also had the lowest muscle inflammation (nuclear NFkB, tissue TNF-alpha) with mitochondrial enzyme activities higher than C (P<0.05 vs C, P = NS vs HF). Under these conditions HFG prevented the HF-associated muscle triglyceride accumulation (P<0.05). The above effects were independent of changes in redox state (total-oxidized glutathione, glutathione peroxidase activity) and were not associated with changes in phosphorylation of AKT and selected AKT targets. Ghrelin administration following high-fat feeding results in a novel model of weight gain with low inflammation, high mitochondrial enzyme activities and normalized triglycerides in skeletal muscle. These effects are independent of changes in tissue redox state and insulin signaling, and they suggest a potential positive metabolic impact of ghrelin in fat-induced obesity
Esperienza ed evento della verità. Pratica filosofica e astrazione scientifica nel pensiero di A.N. Whitehead
No full textThis article analyzes the relationship between philosophy, experience and event in A.N. Whitehead’s thought. From the critics of the concept of object, the author retraces and describes the peculiar “abstract-concrete dialectic”, at the center of the researches concerning the perceptual experience.
Furthermore, according to Whitehead’s later works, she demonstrates how the philosophical practice is different from all other kinds of science, although it requires science itself because of the co-implication of object and event, abstraction and recognition
Il "Guerrin Meschino" di Gesualdo Bufalino : un'"opra" in versi
No full textGesualdo Bufalino first published Il Guerrin Meschino in 1991 in a non-commercial edition. In 1993, after a deep revision, he re-published his work with publisher Bompiani: the novel has a modified plot, and the author decided to insert three new poems in addition to the opening and closing poems, formerly present in 1991’s edition. This paper, in its entirety supported by handwritten material preserved at Fondazione Gesualdo Bufalino (Comiso), is divided in two parts: the first part illustrates the differences between the first and the second edition, the second part provides a critical edition of the five poems
Ghrelin regulates mitochondrial-lipid metabolism gene expression and tissue fat distribution in liver and skeletal muscle.
No full textGhrelin is a gastric hormone increased during caloric restriction and fat depletion. A role of ghrelin in the regulation of lipid and energy metabolism is suggested by fat gain independent of changes in food intake during exogenous ghrelin administration in rodents. We investigated the potential effects of peripheral ghrelin administration (two times daily 200-micrograms [DOSAGE ERROR CORRECTED] sc injection for 4 days) on triglyceride content and mitochondrial and lipid metabolism gene expression in rat liver and muscles. Compared with vehicle, ghrelin increased body weight but not food intake and circulating insulin. In liver, ghrelin induced a lipogenic and glucogenic pattern of gene expression and increased triglyceride content while reducing activated (phosphorylated) stimulator of fatty acid oxidation, AMP-activated protein kinase (AMPK, all P < 0.05), with unchanged mitochondrial oxidative enzyme activities. In contrast, triglyceride content was reduced (P < 0.05) after ghrelin administration in mixed (gastrocnemius) and unchanged in oxidative (soleus) muscle. In mixed muscle, ghrelin increased (P < 0.05) mitochondrial oxidative enzyme activities independent of changes in expression of fat metabolism genes and phosphorylated AMPK. Expression of peroxisome proliferator-activated receptor-gamma, the activation of which reduces muscle fat content, was selectively increased in mixed muscle where it paralleled changes in oxidative capacities (P < 0.05). Thus ghrelin induces tissue-specific changes in mitochondrial and lipid metabolism gene expression and favors triglyceride deposition in liver over skeletal muscle. These novel effects of ghrelin in the regulation of lean tissue fat distribution and metabolism could contribute to metabolic adaptation to caloric restriction and loss of body fat
A FEW WORDS ABOUT THE TEACHER. IN MEMORY OF THE EXCELLENT TEACHER AND SCIENTIST M.R. SAPIN
No full textM.R. Sapin (1925–2015) was a professor at I.M. Sechenov First Moscow State Medical University, honorary academician of Russian academy of medical sciences (1988), and an outstanding representative of the Moscow anatomical school of the middle of XX – early XXI century. From the very beginning of his medical training, Mikhail Romanovich got interested in anatomy, especially in angiology and lymphology, and later concentrated on its studying. The author of more than 30 text books and guidelines for schools, universities, and colleges, Mikhail Romanovich was the doctoral and thesis advisor of 51 Ph.D. and approximately 70 MD dissertations. M.R. Sapin made a significant contribution to the development of anatomical education providing the departments with a various range of dry and wet specimens. His stunning and effective work as a tutor was highly appreciated by his students and colleagues, Russian and foreign anatomists. M.R. Sapin was the leader of the Laboratory of Functional Anatomy since 1972, president of the International Association of Morphologists (1992–2006), chairman of the expert commission of Higher Attestation Commission (1959–1994), etc. A man of a great willpower, M.R. Sapin faced a lot of obstacles in his life and research work but overcame that all successfully. The article presents the most important facts of anatomist’s career and lifetime.</jats:p
Verslag van het voorgevallene tijdens het hooge opperwater op de Nederlandsche rivieren in den winter van 1919 op 1920
No full textNadat de Rijn op 20 December 1919 tot even beneden den middelbaren zomerstand 1901-1910 (M.R.) was gedaald vertoonde zich op die rivier een sterke en vrij snelle was, waardoor het water te Keulen tot 6m +M.R. bij het einde van het jaar steeg. In de daarop volgende 11 dagen daalde de waterstand tot 1.5m +M.R. om daarna in 5 dagen weder te rijzen tot den zeer hoogen stand van 7m +M.R. Door een aanvankelijk sterken later flauweren val liep de stand weder terug tot 1.5m +M.R. op 30 Januari. Ook op de Maas vertoonden zich twee dicht op elkaar volgende sterke wassen. Bij den aanvang van den eersten was op 19 december stond het water te Maastricht op ruim 1m +M.R. en steeg het in 6 dagen tot 4.37m +M.R.; in de daaropvolgende 9 dagen bleef de stand steeds hoger dan 4m +M.R. en daalde toen tot ongeveer 2m +M.R. op 10 Januari 1920. De tweede was trad op laatsgenoemde datum in waarbij het water oplied tot 4.94m +M.R. op 15 Januari. Het water daalde daarna dadelijk en was op 29 Januari tot ongeveer 2m +M.R. weggevallen. Bij den tweeden was op Rijn en Maas werden de hoogst bekende waterstanden bij open rivieren overtroffen te Keulen met 7 cm en te Maastricht met 2 cm. De rivieren waren ijsvrij.Hoogwaterversla
Seismic prevention and rahabilitation of nonstructural elements. Criteria to define a post-seismic damage analysis procedure
No full textIt reports very briefly the research work, developed by the author during her Doctorate course, about a post-seismic damage analysis procedure for nonstructural elements of framed structure building system
The Ontology of the Causal Basis of Man : To the Theory of Time
No full textIn 2014, Amirbegov Y.M.'s monograph titled "Mechanics of Thinking" or "Ontological Basis of the Subject" was published, which dealt with a philosophical question: the relationship of matter with consciousness and time of our being. But since the monograph duplicated the thesis conceived by the author and did not go beyond the material presented in it, on the advice of the consultant the author of the monograph is preparing its second edition in an expanded version with a new title: " Ontology of Causal Basis of Man", attracting his mentor, the author of "The Theory of Time or Principle of Formation of Matter Forms" Amirbegov M.R., as a co-author of this book,
Adhering to the opinion that matter between past and future temporal conti-nuity of eternity is reduced to nothing and in reality there remains only a causeless "Nothing", the authors of this book from a clean sheet, but with baggage of knowledge about objective forms of being and causeless reality with its possibili-ties, build an unparalleled theoretical model of human integrity, answering the question: what does a man, his sensorium, consciousness, his thinking apparatus and thought itself, why and how? The authors submit this book to the judgment of the reader
The Ontology of the Causal Basis of Man : To the Theory of Time
No full textIn 2014, Amirbegov Y.M.'s monograph titled "Mechanics of Thinking" or "Ontological Basis of the Subject" was published, which dealt with a philosophical question: the relationship of matter with consciousness and time of our being. But since the monograph duplicated the thesis conceived by the author and did not go beyond the material presented in it, on the advice of the consultant the author of the monograph is preparing its second edition in an expanded version with a new title: " Ontology of Causal Basis of Man", attracting his mentor, the author of "The Theory of Time or Principle of Formation of Matter Forms" Amirbegov M.R., as a co-author of this book,
Adhering to the opinion that matter between past and future temporal conti-nuity of eternity is reduced to nothing and in reality there remains only a causeless "Nothing", the authors of this book from a clean sheet, but with baggage of knowledge about objective forms of being and causeless reality with its possibili-ties, build an unparalleled theoretical model of human integrity, answering the question: what does a man, his sensorium, consciousness, his thinking apparatus and thought itself, why and how? The authors submit this book to the judgment of the reader
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