196,320 research outputs found
Breve storia dell'ontologia
No full textIl saggio ripercorre le tappe fondamentali del pensiero ontologico occidentale, andando in traccia dell'unico argomento che lo pervade sin dalle sue origini attraversando il pensiero dei pensatori fondamentali
Il monitoraggio della barbabietola da zucchero (Beta vulgaris L.) mediante l'analisi della riflettanza spettrale.
No full textEvaluation of the BiomassBed system in bio-cleaning water contaminated water by fungicides applied in vineyard
No full textBiomassBed is an indirect system designed to collect accidental pesticide spills as well as water remnants of tank sprayers. This system was installed in a vineyard and tested in bio-cleaning water that was contaminated by fungicides in a two-year-field experiment. At the end of each treatment, wastewater of sprayer tanks containing mixtures of cymoxanil, dimethomorph, metalaxyl M, folpet, penconazole, cyprodinil, fludioxonil, azoxystrobin, mandipropamid, pyraclostrobin at different concentrations were discharged into the BiomassBed and repeatedly circulated through an organic biofilter of pruning residues and straw for fungicide adsorption and biodegradation. Water collected was sampled and analysed for fungicide residues, and organic biofilter was analysed to assess fungicide dissipation. Fungicides were removed from water in a range of 92.4–100% of the initial concentration. Metalaxyl M was the least retained and probably desorbed during recirculation of water. However, metalaxyl M residues dissipated in 70 days in collected stagnant water. Abiotic and biotic factors contributing to the disappearance of metalaxyl M in water was hypothesised. Fungicide dissipation in the organic biofilter occurred in a 90-day period with the exception of penconazole that remained at 63.6% and 42.0% in 2008 and 2009, respectively. Owing to the advantageous combination of depuration and bio-degradation processes, fungicides were removed from water in a range of 92.4 to 100%. However more effort is needed to enhance adsorption of mobile fungicides and to achieve a faster degradation of more persistent active ingredients
Donne straniere provenienti da Paesi a Forte Pressione Migratoria e percorso nascita in Umbria.
No full textDONNE STRANIERE PROVENIENTI DA PAESI A FORTE PRESSIONE MIGRATORIA E PERCORSO NASCITA IN UMBRIA
Chiavarini M*, Bernardini I*, Casucci P°, Minelli L*.
* Dipartimento di Specialità Medico Chirurgiche e Sanità Pubblica, Università degli Studi di Perugia
° Direzione Regionale Sanità e Servizi sociali, Regione Umbria
Introduzione.
Nel periodo 2004-2008 la Regione Umbria ha subito un importante cambiamento nella composizione della popolazione: aumento del 4.1% della popolazione residente,di cui 0.3% a carico della popolazione italiana e 60.7% a carico della popolazione immigrata.
Obiettivi.
Descrivere le differenze di accesso e di assistenza nel percorso nascita e negli esiti riproduttivi tra le donne italiane e quelle immigrate.
Metodi.
I dati provengono dai Certificati di Assistenza al Parto di tutte le donne che hanno partorito in Umbria nel 2008. Viene valutata la differenza negli indicatori di assistenza e di esito tra donne straniere provenienti da paesi a forte pressione migratoria (PFPM) e donne di cittadinanza italiana.
Risultati.
Le donne straniere PFPM, che rappresentano il 25% del totale delle madri, sono più giovani delle italiane (età media al parto 27,8 vs 32,2) e possiedono un titolo di studio basso, inferiore o uguale al diploma di scuola media inferiore, ( 45.6% vs17.1%).
Per le donne straniere PFPM, rispetto alle italiane, nell’assistenza alla gravidanza si evidenziano i seguenti punti critici:
- controlli in gravidanza più tardivi (dopo la 12esima settimana) (17.9% vs 4.7%;OR 2.57; IC 95%, 2.21-3.00); basso numero di controlli (≤ 4) (14.6% vs 3.2%; OR 5.22; IC 95%, 4.27-6.36); maggior ricorso ad un taglio cesareo in travaglio rispetto ad uno di elezione (13.3% vs 12.7%; OR 1.49;IC 95%,1.21-1.82); assenza di persone di fiducia al parto (21,1% vs.10.2%; OR 2.35; IC 95%, 1.98-2.79).
Non si registrano differenze significative tra straniere PFPM e italiane per la prematurità (7.2% vs 6.1%) e per neonati con peso <2500 grammi (5.7% vs 6.3%).
Conclusioni.
Nonostante l’offerta capillare e di qualità, accessibile a tutte le donne immigrate, le donne straniere PFPM usufruiscono di una minor assistenza in gravidanza che comunque non inficia gli esiti neonatali. E’tuttavia necessario riorientare i servizi tenendo conto delle caratteristiche socio-culturali della donna immigrata
Cooking Practices in a Central Anatolian Site between the 2nd and the 1st Millennium BC: Fires and Pots at Uşaklı Hӧyük
No full textSince 2008 the investigations at Uşaklı Hӧyük have aimed at reconstructing in detail the history and settlement development of this multi-phase site of the central Anatolian plateau. This paper presents the evidence related to cooking methods spread in central Anatolia during the Late Bronze Age and the Early/Middle Iron Age, with particular emphasis on fire installations and cooking tools found in the recent excavations of Uşaklı Hӧyük. Archaeology supplies a great number of materials and information on cooking and the related pottery and firing equipment: ovens, hearths, andirons, cooking pots and baking plates are a constant presence within the various settlements of the Anatolian plateau. An attempt will be made to identify the functions of the various devices within the different food processing techniques with the aim to reconstruct ancient culinary practices. The morphological analysis of the kitchen utensils and their archaeological context, together with the recent multi-disciplinary approaches (archaeobotanical, zooarchaeological, ethnographic studies, and experimental archaeology), will be able to provide data and information on food preparation and cooking daily life. Therefore,
their analysis may also contribute to identifying some general social and economic dynamics, such as processes of crisis and resilience, changes, and continuity in this critical historical period of the Anatolian Plateau and Ancient Near East
Effects of Tgds knockdown in ATDC5 cells’ chondrogenic differentiation
No full textCatel–Manzke syndrome is a rare autosomal recessive genetic disorder characterized by a symptomatology known as "Pierre-Robin sequence": patients exhibit a small mandible, cleft palate, and a tendency of the tongue to obstruct the airway and digestive tract. Another characteristic is the presence of an additional phalanx affecting the index finger, resulting in finger deviation (clinodactyly). Other malformations have been observed in the cardiac, articular, and other skeletal regions. The syndrome is also associated with growth delay. Catel-Manzke syndrome is related to mutations in the TGDS (dTDP-D-glucose 4,6-dehydratase) gene, located on chromosome 13. The specific function of TGDS in vertebrates is unknown.
We used ATDC5 cells, a well-established model for studying chondrogenesis, to evaluate the effect of TGDS knockdown. ATDC5 TGDS knockdown cells were generated using lentiviral shRNA trasduction (shRNA49). Cells were stimulated with ITS (insulin/transferrin/selenium) for up to 21 days to induce chondrogenesis. Chondrogenic differentiation was assessed by analyzing the expression levels of specific marker genes - SOX9, ACAN, COL2A1, and COL10A1- via quantitative real-time PCR (qPCR).
Quantitative real-time PCR analysis confirmed efficient knockdown of TGDS expression in sh49 cell line compared to the scrambled control (scr). During chondrogenic induction, sh49 knockdown led to a consistent reduction in the expression of key markers, including SOX9, ACAN, COL2A1, and COL10A1, throughout the differentiation time course (days 7, 14, and 21). This indicates an impairment of the chondrogenic process in sh49 cells. As further confirmation of these findings, Alcian Blue staining revealed a marked decrease in proteoglycan production in sh49 cells compared to the scrambled control, consistent with reduced chondrogenic differentiation.
Taken together, these results suggest that TGDS plays a functional role in promoting chondrogenic differentiation
Investigating the role of Tgds in ATDC5 cells’ chondrogenic differentiation
No full textCatel–Manzke syndrome (CMS) is a rare autosomal recessive genetic disorder characterized by a symptomatology known as "Pierre-Robin sequence": patients exhibit a small mandible, cleft palate, and a tendency of the tongue to obstruct the airway and digestive tract. Another characteristic is the presence of an additional phalanx affecting the index finger, resulting in finger deviation (clinodactyly). Other malformations have been observed in the cardiac, articular, and other skeletal regions. CMS is related to mutations in the TGDS (dTDP-D-glucose 4,6-dehydratase) gene, located on chromosome 13. The specific function of TGDS in vertebrates is unknown.
We used ATDC5 cells, a well-established model for studying chondrogenesis, to evaluate the effect of TGDS knockdown. ATDC5 Tgds knockdown cells were generated using lentiviral shRNA trasduction (shRNA49). Cells were stimulated with ITS (insulin/transferrin/selenium) for up to 21 days to induce chondrogenesis. Chondrogenic differentiation was assessed by analyzing the expression levels of specific marker genes - Sox9, Acan, Col2a1, and Col10a1- via RT-qPCR.
RT-qPCR analysis confirmed efficient knockdown of TGDS expression in shRNA49 cell line compared to the control. During chondrogenic induction, shRNA49 knockdown led to a consistent reduction in the expression of key markers - Sox9, Acan, Col2a1, and Col10a1. This indicates an impairment of the chondrogenic process in shRNA49 cells. As further confirmation of these findings, Alcian blue staining revealed a marked decrease in proteoglycan production in shRNA49 cells compared to the scrambled control, consistent with reduced chondrogenic differentiation.
Taken together, these results suggest that TGDS plays a functional role in promoting chondrogenic differentiation
Clinical pharmacology of CAR-T cells: Linking cellular pharmacodynamics to pharmacokinetics and antitumor effects
No full textAdoptive cell transfer of T cells genetically modified with tumor-reactive chimeric antigen receptors (CARs) is a rapidly emerging field in oncology, which in preliminary clinical trials has already shown striking antitumor efficacy. Despite these premises, there are still a number of open issues related to CAR-T cells, spanning from their exact mechanism of action (pharmacodynamics), to the factors associated with their in vivo persistence (pharmacokinetics), and, finally, to the relative contribution of each of the two in determining the antitumor effects and accompanying toxicities. In light of the unprecedented curative potential of CAR-T cells and of their predicted wide availability in the next few years, in this review we will summarize the current knowledge on the clinical pharmacology aspects of what is anticipated to be a brand new class of biopharmaceuticals to join the therapeutic armamentarium of cancer doctors
- …
