1,720,956 research outputs found
Implementation of the quality control system of blood components at the Blood Bank - Western processing hub (Liguria Region - National Health System): analysis of the data of the activity carried out in 2024.
No full textBlood and its components represent an important factor of life for many patients; blood donations in Italy in fact save about 630,000 lives every year. Components are those therapeutic constituents of blood that can be prepared by centrifugation, separation, filtration and freezing. Blood components are: whole blood, leukodepleted red blood cell concentrate, platelet concentrates from buffy coat pools, platelet concentrates from apheresis, fresh frozen plasma from fractionation, plasma from apheresis and factor VIII. Each of them has their own quality standards. The number of quality controls performed corresponds to approximately 1% of total production as required by the ministerial decree of 2/11/2015, except for platelet units that they were evaluated on all units produced. The samples intended for quality control were collected at the Immunohematology and Transfusion Medicine Department of the San Paolo Hospital in Savona - ASL2 Savonese (Liguria Region Health System) and analyzed at the Immunohematology and Transfusion Medicine Department, Clinical Pathology Department (Analysis Laboratory) and S.C. Pathological Anatomy of the San Paolo Hospital in Savona - ASL2 Savonese (Liguria Region Health System) during the year 2024. The data presented suggest a compliant and controlled production process. A maintenance of quality levels is identified in comparison to previous years. A reduction in the presence of residual leukocytes in plasma units is highlighted compared to the year 2023. All units found not to comply with quality controls were discarded except for plasma units which were sent to the plasma derivation industry. Considering the very small number of non-conformities for whole blood and blood components for the year 2024 and the data recorded in previous years, we propose to reduce the quality control sampling from 1% of production to 0.5% for the year 2025. It could be interesting to evaluate possible implementations of waste material for research purposes in the clinical field
A successful healthy childbirth and an ongoing evolutive pregnancy in a case of partial globozoospermia by hyaluronic acid sperm selection
No full textWe here report a successful healthy childbirth and an ongoing evolutive pregnancy in a case of partial globozoospermia after selection of mature spermatozoa bound to hyaluronic acid (HA). The couple underwent two in vitro fertilisation (IVF) cycles. In the first attempt, 14 MII oocytes were retrieved. Randomly, seven oocytes were injected by conventional PVP-ICSI and seven by HA-ICSI. Fertilised oocytes were 2/7 and 4/7 after PVP-ICSI and HA-ICSI respectively. Transfer of two grade A embryos from HA-ICSI lead to birth of a healthy baby. The surplus embryo of the HA-ICSI group was vitrified at blastocyst stage. The two embryos from PVP-ICSI arrested their development. Two years after the childbirth, the vitrified blastocyst was transferred into the uterus, but implant failed. In the second cycle, 14 MII oocytes were retrieved and they were injected by HA-ICSI. Fertilised oocytes were 10 out of 14 injected oocytes. On day 5, two blastocysts were transferred into uterus and a single evolutive pregnancy is ongoing. On day 6, one blastocyst was vitrified. The remaining surplus embryos arrested their development. A healthy childbirth and an ongoing evolutive pregnancy in two consecutive ICSI attempts of the same couple suggest that HA sperm selection might assist in cases with partial globozoospermia
La valutazione delle performance della Morfologia Digitale in Ematologia: confronto con emocitometria automatizzata, microscopia ottica e citofluorimetria.
No full textLa valutazione della morfologia cellulare su striscio di sangue periferico mediante microscopia ottica (MO) viene prescritta dai medici o eseguita dagli specialisti in ematologia a seguito di risultati alterati o allarmi d’immaturità prodotti dagli emocitometri automatizzati. Ad oggi è considerata il gold standard anche se da alcuni anni si sta discutendo circa la possibilità di nuovi standard di riferimento, basati sulla citometria a flusso e sull’uso di opportuni pannelli di anticorpi monoclonali. La valutazione della morfologia cellulare ematologica mostra infatti diversi limiti, tra i quali le competenze richieste ai professionisti, la variabilità intra e inter-osservatore, l’affaticamento e i tempi di analisi prolungati. L’innovazione tecnologica ha permesso lo sviluppo della revisione microscopica automatizzata con sistemi di digitalizzazione e pre-classificazione delle cellule (AIS), sistemi considerati un valido supporto alla diagnostica morfologica. Diversi studi hanno dimostrato le buone performance degli AIS nella pre-classificazione cellulare nei confronti della MO. Ad oggi non vi è evidenza in letteratura di studi di valutazione delle performance degli AIS rispetto al metodo citofluorimentrico. Lo scopo di questo studio è pertanto quello di valutare le prestazioni analitiche della “morfologia digitale” confrontata con il metodo ottico tradizionale e la citofluorimetria. I nostri risultati suggeriscono l’impiego degli AIS come screening morfologico iniziale, che deve essere accompagnato da una verifica al microscopio ottico tradizionale e ad un’analisi multiparametrica di base con il citofluorimetro per i casi dubbi o con incongruenze nella differenziazione leucocitaria.
Traduzione in lingua inglese:
Cell morphology evaluation by optical microscopy (OM) on peripheral blood smears is prescribed by physicians or it’s performed by hematology specialists, following altered results or immaturity alarms of the automated hemocytometer. Even if for years there has been discussions about flow cytometry new standard references and the use of suitable monoclonal panels, today the OM is considered the gold standard. The assessment of hematological cell morphology shows various limits, including the skills required to professionals, observer variability, fatigue and prolonged analysis times. Technological innovation has allowed the development of Automated Information Systems (AIS). They has been considered an effective support for morphological diagnostics. Several studies have shown good performance of AIS in cell pre-classification against OM, but in literature there is no evidence of AIS performance evaluation studies compared to flow cytometric method. The aim of the study is the evaluation of "digital morphology" analytical performance compared to the traditional optical method and flow cytometry. Our results suggest the use of AIS as an initial morphological screening. For doubtful cases or incongruities in leukocyte differentiation, it must be followed by the use of a traditional optical microscope check and a basic multiparametric flow cytometric analysis
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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