181 research outputs found

    Characterization and inhibition of AF10-mediated interaction

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    Hagen S, Mattay D, Raeuber C, Müller K, Arndt KM. Characterization and inhibition of AF10-mediated interaction. Journal of Peptide Science. 2014;20(6):385-397.The non-random chromosomal translocations t(10;11)(p13;q23) and t(10;11)(p13;q14-21) result in leukemogenic fusion proteins comprising the coiled coil domain of the transcription factor AF10 and the proteins MLL or CALM, respectively, and subsequently cause certain types of acute leukemia. The AF10 coiled-coil domain, which is crucial for the leukemogenic effect, has been shown to interact with GAS41, a protein previously identified as the product of an amplified gene in glioblastoma. Using sequential synthetic peptides, we mapped the potential AF10/GAS41 interaction site, which was subsequently be used as scaffold for a library targeting the AF10 coiled-coil domain. Using phage display, we selected a peptide that binds the AF10 coiled-coil domain with higher affinity than the respective coiled-coil region of wild-type GAS41, as demonstrated by phage ELISA, CD, and PCAs. Furthermore, we were able to successfully deploy the inhibitory peptide in a mammalian cell line to lower the expression of Hoxa genes that have been described to be overexpressed in these leukemias. This work dissects molecular determinants mediating AF10-directed interactions in leukemic fusions comprising the N-terminal parts of the proteins MLL or CALM and the C-terminal coiled-coil domain of AF10. Furthermore, it outlines the first steps in recognizing and blocking the leukemia-associated AF10 interaction in histiocytic lymphoma cells and therefore, may have significant implications in future diagnostics and therapeutics. Copyright (c) 2014 European Peptide Society and John Wiley & Sons, Ltd

    Observation of the decays B+Σc(2455)++ΞcB^{+} \to Σ_{c}(2455)^{++} \overlineΞ_{c}^{-} and B0Σc(2455)0Ξc0B^{0} \to Σ_{c}(2455)^{0} \overlineΞ_{c}^{0}

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    International audienceWe report the first observation of the two-body baryonic decays B+Σc(2455)++ΞcB^{+} \to Σ_{c}(2455)^{++} \overlineΞ_{c}^{-} and B0Σc(2455)0Ξc0B^{0} \to Σ_{c}(2455)^{0} \overlineΞ_{c}^{0} with significances of 7.3σ7.3\,σ and 6.2σ6.2\,σ, respectively, including statistical and systematic uncertainties. The branching fractions are measured to be B(B+Σc(2455)++Ξc)=(5.74±1.11±0.421.53+2.47)×104\mathcal{B}(B^{+} \to Σ_{c}(2455)^{++} \overlineΞ_{c}^{-}) = (5.74 \pm 1.11 \pm 0.42_{-1.53}^{+2.47}) \times 10^{-4} and B(B0Σc(2455)0Ξc0)=(4.83±1.12±0.370.60+0.72)×104\mathcal{B}(B^{0} \to Σ_{c}(2455)^{0} \overlineΞ_{c}^{0}) = (4.83 \pm 1.12 \pm 0.37_{-0.60}^{+0.72}) \times 10^{-4}. The first and second uncertainties are statistical and systematic, respectively, while the third ones arise from the absolute branching fractions of Ξc\overlineΞ_{c}^{-} or Ξc0\overlineΞ_{c}^{0} decays. The data samples used for this analysis have integrated luminosities of 711~fb1\mathrm{fb}^{-1} and 365~fb1\mathrm{fb}^{-1}, and were collected at the Υ(4S)Υ(4S) resonance by the Belle and Belle~II detectors operating at the KEKB and SuperKEKB asymmetric-energy e+ee^{+}e^{-} colliders, respectively

    Measurements of the branching fractions of Ξc+Σ+KS0{\Xi }_{c}^{+}\to {\Sigma }^{+}{K}_{S}^{0}, Ξc+Ξ0π+{\Xi }_{c}^{+}\to {\Xi }^{0}{\pi }^{+}, and Ξc+Ξ0K+{\Xi }_{c}^{+}\to {\Xi }^{0}{K}+ at Belle and Belle II

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    International audienceUsing 983.0 fb1\rm{fb}^{-1} and 427.9 fb1\rm{fb}^{-1} data samples collected with the Belle and Belle II detectors at the KEKB and SuperKEKB asymmetric energy e+ee^+e^- colliders, respectively, we present studies of the Cabibbo-favored Ξc+Ξ_c^+ decays Ξc+Σ+KS0{Ξ_{c}^{+}\to Σ^{+}K_{S}^{0}} and Ξc+Ξ0π+Ξ_{c}^{+}\to Ξ^{0}π^{+}, and the singly Cabibbo-suppressed decay Ξc+Ξ0K+Ξ_{c}^{+}\to Ξ^{0}K^{+}. The ratios of branching fractions of Ξc+Σ+KS0{Ξ_{c}^{+}\to Σ^{+}K_{S}^{0}} and Ξc+Ξ0K+Ξ_{c}^{+}\to Ξ^{0}K^{+} relative to that of Ξc+Ξπ+π+Ξ_{c}^{+}\toΞ^{-}π^{+}π^{+} are measured for the first time, while the ratio B(Ξc+Ξ0π+)/B(Ξc+Ξπ+π+){\cal B}(Ξ_{c}^{+}\toΞ^{0}π^{+})/{\cal B}(Ξ_{c}^{+}\toΞ^{-}π^{+}π^{+}) is also determined and improved by an order of magnitude in precision. The measured branching fraction ratios are B(Ξc+Σ+KS0)B(Ξc+Ξπ+π+)=0.067±0.007±0.003\frac{\cal{B}(Ξ_{c}^{+} \to Σ^{+}K_{S}^{0})}{\cal{B}(Ξ_{c}^{+}\to Ξ^{-}π^{+}π^+)}= 0.067 \pm 0.007 \pm 0.003, B(Ξc+Ξ0π+)B(Ξc+Ξπ+π+)=0.251±0.005±0.010\frac{\cal{B}(Ξ_c^{+} \to Ξ^{0}π^{+})}{\cal{B}(Ξ_{c}^{+}\to Ξ^{-}π^{+}π^+)} = 0.251 \pm 0.005 \pm 0.010, B(Ξc+Ξ0K+)B(Ξc+Ξπ+π+)=0.017±0.003±0.001\frac{\cal{B}(Ξ_c^{+} \to Ξ^{0}K^{+})}{\cal{B}(Ξ_{c}^{+}\to Ξ^{-}π^{+}π^+)} = 0.017 \pm 0.003 \pm 0.001. Additionally, the ratio B(Ξc+Ξ0K+)/B(Ξc+Ξ0π+){\cal B}(Ξ_{c}^{+}\toΞ^{0}K^{+})/{\cal B}(Ξ_{c}^{+}\toΞ^{0}π^{+}) is measured to be 0.068±0.010±0.004 0.068 \pm 0.010 \pm 0.004. Here, the first and second uncertainties are statistical and systematic, respectively. Multiplying the ratios by the branching fraction of the normalization mode, B(Ξc+Ξπ+π+)=(2.9±1.3)%{\mathcal B}(Ξ_{c}^{+}\toΞ^{-}π^{+}π^+)= (2.9\pm 1.3)\%, we obtain the following absolute branching fractions B(Ξc+Σ+KS0)=(0.194±0.021±0.009±0.087){\cal B}(Ξ_{c}^{+}\toΣ^{+}K^{0}_{S}) = (0.194 \pm 0.021 \pm 0.009 \pm 0.087 )%, B(Ξc+Ξ0π+)=(0.728±0.014±0.027±0.326){\cal B}(Ξ_{c}^{+}\toΞ^{0}π^{+}) = (0.728 \pm 0.014 \pm 0.027 \pm 0.326 )%, B(Ξc+Ξ0K+)=(0.049±0.007±0.003±0.022){\cal B}(Ξ_{c}^{+}\toΞ^{0}K^{+}) = (0.049 \pm 0.007 \pm 0.003 \pm 0.022 )%

    Observation of the decays B+Σc(2455)++ΞcB^{+} \to Σ_{c}(2455)^{++} \overlineΞ_{c}^{-} and B0Σc(2455)0Ξc0B^{0} \to Σ_{c}(2455)^{0} \overlineΞ_{c}^{0}

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    We report the first observation of the two-body baryonic decays B+Σc(2455)++ΞcB^{+} \to Σ_{c}(2455)^{++} \overlineΞ_{c}^{-} and B0Σc(2455)0Ξc0B^{0} \to Σ_{c}(2455)^{0} \overlineΞ_{c}^{0} with significances of 7.3σ7.3\,σ and 6.2σ6.2\,σ, respectively, including statistical and systematic uncertainties. The branching fractions are measured to be B(B+Σc(2455)++Ξc)=(5.74±1.11±0.421.53+2.47)×104\mathcal{B}(B^{+} \to Σ_{c}(2455)^{++} \overlineΞ_{c}^{-}) = (5.74 \pm 1.11 \pm 0.42_{-1.53}^{+2.47}) \times 10^{-4} and B(B0Σc(2455)0Ξc0)=(4.83±1.12±0.370.60+0.72)×104\mathcal{B}(B^{0} \to Σ_{c}(2455)^{0} \overlineΞ_{c}^{0}) = (4.83 \pm 1.12 \pm 0.37_{-0.60}^{+0.72}) \times 10^{-4}. The first and second uncertainties are statistical and systematic, respectively, while the third ones arise from the absolute branching fractions of Ξc\overlineΞ_{c}^{-} or Ξc0\overlineΞ_{c}^{0} decays. The data samples used for this analysis have integrated luminosities of 711~fb1\mathrm{fb}^{-1} and 365~fb1\mathrm{fb}^{-1}, and were collected at the Υ(4S)Υ(4S) resonance by the Belle and Belle~II detectors operating at the KEKB and SuperKEKB asymmetric-energy e+ee^{+}e^{-} colliders, respectively

    Silicium-Rampen-Wechselwirkung Abschlussbericht

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    Main items of the first part were the search for suitable ramp materials, the accomplishment of test crystallizations at Heliotronic, the characterization of the products, and the production of test solar cells. PyroC-coated graphite and silicon plates with surface texture are useful ramp materials. Silicon foils of a thickness of 200 to 300 #mu#m and with good geometry were successfully grown. Grain sizes were between 10 and several hundred #mu#m, dislocation density between 10"6 and 10"7/cm"2. Solar cells were produced showing efficiencies up to about 10% with ARC. The graphite substrates could be re-used many times whereas the textured silicon ramps showed some surface damage. The CVD deposition which was studied in the second part of the project was performed in a home-built CVD reactor with optical heating. Substrates of a size of 15 x 50 mm"2 were coated with a Si layer of a thickness of 200-300 #mu#m which could be separated from the substrate. Very high deposition rates of 8 to 10 #mu#m/min at 1300 C could be demonstrated. The as-deposited layers were fine-grained and had to be recrystallized to improve the quality. First solar cells made from these sheets showed a maximum efficiency of 9.6%. (orig./MM)Aufgabe des Teilprogramms RAFT-Rampen war die Suche nach geeigneten Materialien, die Durchfuehrung von Testabguessen bei der Firma Heliotronic, die Materialcharakterisierung sowie die Herstellung von Testsolarzellen. Es stellte sich heraus, das pyroC-beschichtete Graphit- und strukturierte Si-Rampen fuer den RAFT-Prozess geeignet sind. Es koennen Silicumfolien von ca. 200-300 #mu#m Dicke mit definierter Geometrie, glatter Oberflaeche, Korngroesse zwischen 10 #mu#m und einigen 100 #mu#m und Versetzungsdichten im Bereich 10"6-10"7/cm"2 hergestellt werden. Bei daraus gefertigten Solarzellen wurden Wirkungsgrade bis gegen 10% (mit AR-Schicht) erreicht. PyroC-beschichtete Graphit-Rampen sind oft wiederverwendbar, strukturierte Silicium-Rampen waren nach den Abguessen meist beschaedigt. Die Versuche zum zweiten Teilprogramm wurden mit einer selbst entwickelten Normaldruck-CVD-Analge mit optischer Heizung durchgefuehrt. Auf Substraten der Groesse 15 x 50 mm"2 wurden Schichten von 200-300 #mu# Dicke hergestellt. Die Abscheiderate war mit 8-10 #mu#m/min bei einer Substrat-Temperatur von ca. 1300 C sehr hoch. Es gelang, geeignete Substrate zu finden, die ein einwandfreies Abloesen der Siliciumfolie zeigten. Die abgeschiedenen Schichten waren feinkristallin und wurden deshalb rekristallisiert. Erste Solarzellen aus diesem Material zeigten Wirkungsgrade bis zu 9,6%. (orig./MM)SIGLEAvailable from TIB Hannover: F95B1893+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman

    Measurements of the branching fractions of Ξc+Σ+KS0\Xi_{c}^{+}\to \Sigma^{+}K_{S}^{0}, Ξc+Ξ0π+\Xi_{c}^{+}\to \Xi^{0}\pi^{+}, and Ξc+Ξ0K+\Xi_{c}^{+}\to \Xi^{0}K^{+} at Belle and Belle II

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    Using 983.0 fb1\rm{fb}^{-1} and 427.9 fb1\rm{fb}^{-1} data samples collected with the Belle and Belle II detectors at the KEKB and SuperKEKB asymmetric energy e+ee^+e^- colliders, respectively, we present studies of the Cabibbo-favored Ξc+\Xi_c^+ decays Ξc+Σ+KS0{\Xi_{c}^{+}\to \Sigma^{+}K_{S}^{0}} and Ξc+Ξ0π+\Xi_{c}^{+}\to \Xi^{0}\pi^{+}, and the singly Cabibbo-suppressed decay Ξc+Ξ0K+\Xi_{c}^{+}\to \Xi^{0}K^{+}. The ratios of branching fractions of Ξc+Σ+KS0{\Xi_{c}^{+}\to \Sigma^{+}K_{S}^{0}} and Ξc+Ξ0K+\Xi_{c}^{+}\to \Xi^{0}K^{+} relative to that of Ξc+Ξπ+π+\Xi_{c}^{+}\to\Xi^{-}\pi^{+}\pi^{+} are measured for the first time, while the ratio B(Ξc+Ξ0π+)/B(Ξc+Ξπ+π+){\cal B}(\Xi_{c}^{+}\to\Xi^{0}\pi^{+})/{\cal B}(\Xi_{c}^{+}\to\Xi^{-}\pi^{+}\pi^{+}) is also determined and improved by an order of magnitude in precision. The measured branching fraction ratios are B(Ξc+Σ+KS0)B(Ξc+Ξπ+π+)=0.067±0.007±0.003\frac{\cal{B}(\Xi_{c}^{+} \to \Sigma^{+}K_{S}^{0})}{\cal{B}(\Xi_{c}^{+}\to \Xi^{-}\pi^{+}\pi^+)}= 0.067 \pm 0.007 \pm 0.003, B(Ξc+Ξ0π+)B(Ξc+Ξπ+π+)=0.248±0.005±0.009\frac{\cal{B}(\Xi_c^{+} \to \Xi^{0}\pi^{+})}{\cal{B}(\Xi_{c}^{+}\to \Xi^{-}\pi^{+}\pi^+)} = 0.248 \pm 0.005 \pm 0.009, B(Ξc+Ξ0K+)B(Ξc+Ξπ+π+)=0.017±0.003±0.001\frac{\cal{B}(\Xi_c^{+} \to \Xi^{0}K^{+})}{\cal{B}(\Xi_{c}^{+}\to \Xi^{-}\pi^{+}\pi^+)} = 0.017 \pm 0.003 \pm 0.001. Additionally, the ratio B(Ξc+Ξ0K+)/B(Ξc+Ξ0π+){\cal B}(\Xi_{c}^{+}\to\Xi^{0}K^{+})/{\cal B}(\Xi_{c}^{+}\to\Xi^{0}\pi^{+}) is measured to be 0.068±0.010±0.004 0.068 \pm 0.010 \pm 0.004. Here, the first and second uncertainties are statistical and systematic, respectively. Multiplying the ratios by the branching fraction of the normalization mode, B(Ξc+Ξπ+π+)=(2.9±1.3)%{\mathcal B}(\Xi_{c}^{+}\to\Xi^{-}\pi^{+}\pi^+)= (2.9\pm 1.3)\%, we obtain the following absolute branching fractions B(Ξc+Σ+KS0)=(0.194±0.021±0.009±0.087){\cal B}(\Xi_{c}^{+}\to\Sigma^{+}K^{0}_{S}) = (0.194 \pm 0.021 \pm 0.009 \pm 0.087 )%, B(Ξc+Ξ0π+)=(0.719±0.014±0.024±0.322){\cal B}(\Xi_{c}^{+}\to\Xi^{0}\pi^{+}) = (0.719 \pm 0.014 \pm 0.024 \pm 0.322 )%, B(Ξc+Ξ0K+)=(0.049±0.007±0.002±0.022){\cal B}(\Xi_{c}^{+}\to\Xi^{0}K^{+}) = (0.049 \pm 0.007 \pm 0.002 \pm 0.022 )%

    Measurements of the branching fractions of Ξc0Ξ0π0\Xi_{c}^{0}\to\Xi^{0}\pi^{0}, Ξc0Ξ0η\Xi_{c}^{0}\to\Xi^{0}\eta, and Ξc0Ξ0η\Xi_{c}^{0}\to\Xi^{0}\eta^{\prime} and asymmetry parameter of Ξc0Ξ0π0\Xi_{c}^{0}\to\Xi^{0}\pi^{0}

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    We present a study of Ξc0Ξ0π0\Xi_{c}^{0}\to\Xi^{0}\pi^{0}, Ξc0Ξ0η\Xi_{c}^{0}\to\Xi^{0}\eta, and Ξc0Ξ0η\Xi_{c}^{0}\to\Xi^{0}\eta^{\prime} decays using the Belle and Belle~II data samples, which have integrated luminosities of 980~fb1\mathrm{fb}^{-1} and 426~fb1\mathrm{fb}^{-1}, respectively. We measure the following relative branching fractions B(Ξc0Ξ0π0)/B(Ξc0Ξπ+)=0.48±0.02(stat)±0.03(syst),{\cal B}(\Xi_{c}^{0}\to\Xi^{0}\pi^{0})/{\cal B}(\Xi_{c}^{0}\to\Xi^{-}\pi^{+}) = 0.48 \pm 0.02 ({\rm stat}) \pm 0.03 ({\rm syst}) , B(Ξc0Ξ0η)/B(Ξc0Ξπ+)=0.11±0.01(stat)±0.01(syst),{\cal B}(\Xi_{c}^{0}\to\Xi^{0}\eta)/{\cal B}(\Xi_{c}^{0}\to\Xi^{-}\pi^{+}) = 0.11 \pm 0.01 ({\rm stat}) \pm 0.01 ({\rm syst}) , B(Ξc0Ξ0η)/B(Ξc0Ξπ+)=0.08±0.02(stat)±0.01(syst){\cal B}(\Xi_{c}^{0}\to\Xi^{0}\eta^{\prime})/{\cal B}(\Xi_{c}^{0}\to\Xi^{-}\pi^{+}) = 0.08 \pm 0.02 ({\rm stat}) \pm 0.01 ({\rm syst}) for the first time, where the uncertainties are statistical (stat\rm stat) and systematic (syst\rm syst). By multiplying by the branching fraction of the normalization mode, B(Ξc0Ξπ+){\mathcal B}(\Xi_{c}^{0}\to\Xi^{-}\pi^{+}), we obtain the following absolute branching fraction results (6.9±0.3(stat)±0.5(syst)±1.3(norm))×103(6.9 \pm 0.3 ({\rm stat}) \pm 0.5 ({\rm syst}) \pm 1.3 ({\rm norm})) \times 10^{-3}, (1.6±0.2(stat)±0.2(syst)±0.3(norm))×103(1.6 \pm 0.2 ({\rm stat}) \pm 0.2 ({\rm syst}) \pm 0.3 ({\rm norm})) \times 10^{-3}, and (1.2±0.3(stat)±0.1(syst)±0.2(norm))×103(1.2 \pm 0.3 ({\rm stat}) \pm 0.1 ({\rm syst}) \pm 0.2 ({\rm norm})) \times 10^{-3}, for Ξc0\Xi_{c}^{0} decays to Ξ0π0\Xi^{0}\pi^{0}, Ξ0η\Xi^{0}\eta, and Ξ0η\Xi^{0}\eta^{\prime} final states, respectively. The third errors are from the uncertainty on B(Ξc0Ξπ+){\mathcal B}(\Xi_{c}^{0}\to\Xi^{-}\pi^{+}). The asymmetry parameter for Ξc0Ξ0π0\Xi_{c}^{0}\to\Xi^{0}\pi^{0} is measured to be α(Ξc0Ξ0π0)=0.90±0.15(stat)±0.23(syst)\alpha(\Xi_{c}^{0}\to\Xi^{0}\pi^{0}) = -0.90\pm0.15({\rm stat})\pm0.23({\rm syst})

    The French Invasions of Portugal 1807-1811: rebellion, reaction and resistance

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    Portugal’s involvement in the Revolutionary and Napoleonic wars resulted in substantial economic, political and social change revealing interconnections between state and economy that have not been acknowledged fully within the existing literature. On the one hand, economic and political change was precipitated by the flight of Dom João, the removal of the court to Rio de Janeiro, and the appointment of a regency council in Lisbon: events that were the result of much more than the mere confluence of external drivers and internal pressures in Europe, however complex and compelling they may have been at the time. Although governance in Portugal had been handed over to the regency council strict limitations were imposed on its autonomy. Once Lisbon was occupied, and French military government imposed on Portugal, her continued role as entrepôt, linking the South Atlantic economy to that of Europe, could not be guaranteed. Brazil’s ports were therefore opened to foreign vessels and restrictions on agriculture, manufacture and inter-regional trade in the colonies were lifted presaging a transition from neo-mercantilism to proto-industrialised capitalism. The meanings of this dislocation of political power and the shift of government from metropolis to colony were complex, not least in relation to the location and limits of absolutist authority. The immediate results of which were a series of popular insurrections in Portugal, a swift response by the French military government and conservative reaction by Portuguese élites, leading to widespread popular resistance in 1808 and 1809 and, subsequently, Portugal’s wholesale involvement in the Peninsular War with severe and deleterious effects on the Portuguese population and economy. Ultimately, these events would lead to demands for constitutional reform and civil war but not, as yet, the dismantling of mercantilism, the abolition of slavery or the separation of Portugal and Brazil as independent states. Ironically, the forces for change in this regard, in the years immediately following the Napoleonic Wars, would appear stronger in the metropolis and weaker in its former colony

    Measurement of C ⁣PC\!P violation in B0KS0π0B^{0}\rightarrow K_{S}^{0}\pi^{0} decays at Belle II

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    We report a measurement of the C ⁣PC\!P-violating parameters AA and SS in B0KS0π0B^{0}\to K_{S}^{0} \pi^{0} decays at Belle II using a sample of 387×106387\times 10^{6} BBˉB\bar{B} events recorded in e+ee^{+}e^{-} collisions at a center-of-mass energy corresponding to the Υ(4S)\Upsilon(4S) resonance. These parameters are determined by fitting the proper decay-time distribution of a sample of 415 signal events. We obtain A=0.040.14+0.15±0.05A = 0.04^{+0.15}_{-0.14}\pm 0.05 and S=0.750.23+0.20±0.04S = 0.75^{+0.20}_{-0.23}\pm 0.04, where the first uncertainties are statistical and the second are systematic

    Measurement of C ⁣PC\!P asymmetries and branching-fraction ratios for B±DK±B^\pm \to DK^\pm and Dπ±D\pi^\pm with DKS0K±πD\to K^0_{\rm S} K^\pm\pi^\mp using Belle and Belle II data

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    We measure C ⁣PC\!P asymmetries and branching-fraction ratios for B±DK±B^\pm \to DK^\pm and Dπ±D\pi^\pm decays with DKS0K±πD\to K^0_{\rm S} K^\pm\pi^\mp, where DD is a superposition of D0D^0 and Dˉ0\bar{D}^0. We use the full data set of the Belle experiment, containing 772×106 BBˉ772\times 10^6~B\bar{B} pairs, and data from the Belle~II experiment, containing 387×106 BBˉ387\times 10^6~B\bar{B} pairs, both collected in electron-positron collisions at the Υ(4S)\Upsilon(4S) resonance. Our results provide model-independent information on the unitarity triangle angle ϕ3\phi_3.Comment: 26 pages, 8 figure
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