181 research outputs found
Characterization and inhibition of AF10-mediated interaction
Hagen S, Mattay D, Raeuber C, Müller K, Arndt KM. Characterization and inhibition of AF10-mediated interaction. Journal of Peptide Science. 2014;20(6):385-397.The non-random chromosomal translocations t(10;11)(p13;q23) and t(10;11)(p13;q14-21) result in leukemogenic fusion proteins comprising the coiled coil domain of the transcription factor AF10 and the proteins MLL or CALM, respectively, and subsequently cause certain types of acute leukemia. The AF10 coiled-coil domain, which is crucial for the leukemogenic effect, has been shown to interact with GAS41, a protein previously identified as the product of an amplified gene in glioblastoma. Using sequential synthetic peptides, we mapped the potential AF10/GAS41 interaction site, which was subsequently be used as scaffold for a library targeting the AF10 coiled-coil domain. Using phage display, we selected a peptide that binds the AF10 coiled-coil domain with higher affinity than the respective coiled-coil region of wild-type GAS41, as demonstrated by phage ELISA, CD, and PCAs. Furthermore, we were able to successfully deploy the inhibitory peptide in a mammalian cell line to lower the expression of Hoxa genes that have been described to be overexpressed in these leukemias. This work dissects molecular determinants mediating AF10-directed interactions in leukemic fusions comprising the N-terminal parts of the proteins MLL or CALM and the C-terminal coiled-coil domain of AF10. Furthermore, it outlines the first steps in recognizing and blocking the leukemia-associated AF10 interaction in histiocytic lymphoma cells and therefore, may have significant implications in future diagnostics and therapeutics. Copyright (c) 2014 European Peptide Society and John Wiley & Sons, Ltd
Observation of the decays and
International audienceWe report the first observation of the two-body baryonic decays and with significances of and , respectively, including statistical and systematic uncertainties. The branching fractions are measured to be and . The first and second uncertainties are statistical and systematic, respectively, while the third ones arise from the absolute branching fractions of or decays. The data samples used for this analysis have integrated luminosities of 711~ and 365~, and were collected at the resonance by the Belle and Belle~II detectors operating at the KEKB and SuperKEKB asymmetric-energy colliders, respectively
Measurements of the branching fractions of , , and at Belle and Belle II
International audienceUsing 983.0 and 427.9 data samples collected with the Belle and Belle II detectors at the KEKB and SuperKEKB asymmetric energy colliders, respectively, we present studies of the Cabibbo-favored decays and , and the singly Cabibbo-suppressed decay . The ratios of branching fractions of and relative to that of are measured for the first time, while the ratio is also determined and improved by an order of magnitude in precision. The measured branching fraction ratios are , , . Additionally, the ratio is measured to be . Here, the first and second uncertainties are statistical and systematic, respectively. Multiplying the ratios by the branching fraction of the normalization mode, , we obtain the following absolute branching fractions , ,
Observation of the decays and
We report the first observation of the two-body baryonic decays and with significances of and , respectively, including statistical and systematic uncertainties. The branching fractions are measured to be and . The first and second uncertainties are statistical and systematic, respectively, while the third ones arise from the absolute branching fractions of or decays. The data samples used for this analysis have integrated luminosities of 711~ and 365~, and were collected at the resonance by the Belle and Belle~II detectors operating at the KEKB and SuperKEKB asymmetric-energy colliders, respectively
Silicium-Rampen-Wechselwirkung Abschlussbericht
Main items of the first part were the search for suitable ramp materials, the accomplishment of test crystallizations at Heliotronic, the characterization of the products, and the production of test solar cells. PyroC-coated graphite and silicon plates with surface texture are useful ramp materials. Silicon foils of a thickness of 200 to 300 #mu#m and with good geometry were successfully grown. Grain sizes were between 10 and several hundred #mu#m, dislocation density between 10"6 and 10"7/cm"2. Solar cells were produced showing efficiencies up to about 10% with ARC. The graphite substrates could be re-used many times whereas the textured silicon ramps showed some surface damage. The CVD deposition which was studied in the second part of the project was performed in a home-built CVD reactor with optical heating. Substrates of a size of 15 x 50 mm"2 were coated with a Si layer of a thickness of 200-300 #mu#m which could be separated from the substrate. Very high deposition rates of 8 to 10 #mu#m/min at 1300 C could be demonstrated. The as-deposited layers were fine-grained and had to be recrystallized to improve the quality. First solar cells made from these sheets showed a maximum efficiency of 9.6%. (orig./MM)Aufgabe des Teilprogramms RAFT-Rampen war die Suche nach geeigneten Materialien, die Durchfuehrung von Testabguessen bei der Firma Heliotronic, die Materialcharakterisierung sowie die Herstellung von Testsolarzellen. Es stellte sich heraus, das pyroC-beschichtete Graphit- und strukturierte Si-Rampen fuer den RAFT-Prozess geeignet sind. Es koennen Silicumfolien von ca. 200-300 #mu#m Dicke mit definierter Geometrie, glatter Oberflaeche, Korngroesse zwischen 10 #mu#m und einigen 100 #mu#m und Versetzungsdichten im Bereich 10"6-10"7/cm"2 hergestellt werden. Bei daraus gefertigten Solarzellen wurden Wirkungsgrade bis gegen 10% (mit AR-Schicht) erreicht. PyroC-beschichtete Graphit-Rampen sind oft wiederverwendbar, strukturierte Silicium-Rampen waren nach den Abguessen meist beschaedigt. Die Versuche zum zweiten Teilprogramm wurden mit einer selbst entwickelten Normaldruck-CVD-Analge mit optischer Heizung durchgefuehrt. Auf Substraten der Groesse 15 x 50 mm"2 wurden Schichten von 200-300 #mu# Dicke hergestellt. Die Abscheiderate war mit 8-10 #mu#m/min bei einer Substrat-Temperatur von ca. 1300 C sehr hoch. Es gelang, geeignete Substrate zu finden, die ein einwandfreies Abloesen der Siliciumfolie zeigten. Die abgeschiedenen Schichten waren feinkristallin und wurden deshalb rekristallisiert. Erste Solarzellen aus diesem Material zeigten Wirkungsgrade bis zu 9,6%. (orig./MM)SIGLEAvailable from TIB Hannover: F95B1893+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman
Measurements of the branching fractions of , , and at Belle and Belle II
Using 983.0 and 427.9 data samples collected with the Belle and Belle II detectors at the KEKB and SuperKEKB asymmetric energy colliders, respectively, we present studies of the Cabibbo-favored decays and , and the singly Cabibbo-suppressed decay . The ratios of branching fractions of and relative to that of are measured for the first time, while the ratio is also determined and improved by an order of magnitude in precision. The measured branching fraction ratios are , , . Additionally, the ratio is measured to be . Here, the first and second uncertainties are statistical and systematic, respectively. Multiplying the ratios by the branching fraction of the normalization mode, , we obtain the following absolute branching fractions , ,
Measurements of the branching fractions of , , and and asymmetry parameter of
We present a study of , , and decays using the Belle and Belle~II data samples, which have integrated luminosities of 980~ and 426~, respectively. We measure the following relative branching fractions for the first time, where the uncertainties are statistical () and systematic (). By multiplying by the branching fraction of the normalization mode, , we obtain the following absolute branching fraction results , , and , for decays to , , and final states, respectively. The third errors are from the uncertainty on . The asymmetry parameter for is measured to be
The French Invasions of Portugal 1807-1811: rebellion, reaction and resistance
Portugal’s involvement in the Revolutionary and Napoleonic wars resulted in substantial economic, political and social change revealing interconnections between state and economy that have not been acknowledged fully within the existing literature. On the one hand, economic and political change was precipitated by the flight of Dom João, the removal of the court to Rio de Janeiro, and the appointment of a regency council in Lisbon: events that were the result of much more than the mere confluence of external drivers and internal pressures in Europe, however complex and compelling they may have been at the time. Although governance in Portugal had been handed over to the regency council strict limitations were imposed on its autonomy. Once Lisbon was occupied, and French military government imposed on Portugal, her continued role as entrepôt, linking the South Atlantic economy to that of Europe, could not be guaranteed. Brazil’s ports were therefore opened to foreign vessels and restrictions on agriculture, manufacture and inter-regional trade in the colonies were lifted presaging a transition from neo-mercantilism to proto-industrialised capitalism. The meanings of this dislocation of political power and the shift of government from metropolis to colony were complex, not least in relation to the location and limits of absolutist authority. The immediate results of which were a series of popular insurrections in Portugal, a swift response by the French military government and conservative reaction by Portuguese élites, leading to widespread popular resistance in 1808 and 1809 and, subsequently, Portugal’s wholesale involvement in the Peninsular War with severe and deleterious effects on the Portuguese population and economy. Ultimately, these events would lead to demands for constitutional reform and civil war but not, as yet, the dismantling of mercantilism, the abolition of slavery or the separation of Portugal and Brazil as independent states. Ironically, the forces for change in this regard, in the years immediately following the Napoleonic Wars, would appear stronger in the metropolis and weaker in its former colony
Measurement of violation in decays at Belle II
We report a measurement of the -violating parameters and in
decays at Belle II using a sample of events recorded in collisions at a
center-of-mass energy corresponding to the resonance. These
parameters are determined by fitting the proper decay-time distribution of a
sample of 415 signal events. We obtain and
, where the first uncertainties are
statistical and the second are systematic
Measurement of asymmetries and branching-fraction ratios for and with using Belle and Belle II data
We measure asymmetries and branching-fraction ratios for and decays with , where
is a superposition of and . We use the full data set of the
Belle experiment, containing pairs, and data from the
Belle~II experiment, containing pairs, both collected
in electron-positron collisions at the resonance. Our results
provide model-independent information on the unitarity triangle angle .Comment: 26 pages, 8 figure
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