1,490 research outputs found
Gender differences in healthy life years within the EU: an exploration of the "health-survival" paradox.
No full textvan Oyen H, Nuselder W, Jagger C, Kolip P, Cambois E, Robine JM. Gender differences in healthy life years within the EU: an exploration of the "health-survival" paradox. International Journal of Public Health. 2013;58(1):143-155
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JAGGER localization and function are dependent on GPI anchor addition
No full textKey message: GPI anchor addition is important for JAGGER localization and in vivo function. Loss of correct GPI anchor addition in JAGGER, negatively affects its localization and function. Abstract: In flowering plants, successful double fertilization requires the correct delivery of two sperm cells to the female gametophyte inside the ovule. The delivery of a single pair of sperm cells is achieved by the entrance of a single pollen tube into one female gametophyte. To prevent polyspermy, Arabidopsis ovules avoid the attraction of multiple pollen tubes to one ovule–polytubey block. In Arabidopsis jagger mutants, a significant number of ovules attract more than one pollen tube to an ovule due to an impairment in synergid degeneration. JAGGER encodes a putative arabinogalactan protein which is predicted to be anchored to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. Here, we show that JAGGER fused to citrine yellow fluorescent protein (JAGGER-cYFP) is functional and localizes mostly to the periphery of ovule integuments and transmitting tract cells. We further investigated the importance of GPI-anchor addition domains for JAGGER localization and function. Different JAGGER proteins with deletions in predicted ω-site regions and GPI attachment signal domain, expected to compromise the addition of the GPI anchor, led to disruption of JAGGER localization in the cell periphery. All JAGGER proteins with disrupted localization were also not able to rescue the polytubey phenotype, pointing to the importance of GPI-anchor addition to in vivo function of the JAGGER protein. © 2024, The Author(s).Open access articleThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
Overcoming barries to compliance
No full textJanine Jagger, PhD, is an epidemiologist specializing in injury prevention and control. Early in her career, her research and advocacy focused on brain trauma and motor vehicle safety.
Over the last 20 years, Dr. Jagger has devoted herself to reducing healthcare workplace transmission of bloodborne pathogens. In 1988, Dr. Jagger and colleagues published a landmark study in the New England Journal of Medicine which detailed the characteristics of medical devices causing needlestick injuries, and criteria for protective needle designs. This pioneering research provided the foundation for the development of a new generation of safer medical devices.
In 1991, Dr. Jagger developed the EPINet surveillance system to provide healthcare facilities with a standardized system for tracking needlestick injuries and blood and body fluid exposures; it is now used by over 1,500 healthcare facilities in the US, and in more than 50 countries around the world. The worldwide dissemination of EPINet has resulted in worldwide access to data on the causes and prevention of healthcare workplace exposures to bloodborne pathogens.
In 2002, Dr. Jagger received one of the most prestigious awards in the US: a MacArthur Foundation fellowship. The award is given to individuals who have shown extraordinary originality and dedication in their professional pursuits.
Dr. Jagger and a team of colleagues are the inventors of six patented safety needle devices, which were honored with a Distinguished Inventor Award in 1988 by Intellectual Property Owners, Inc., and displayed by the US Patent and Trademark Office in its 1990 Bicentennial Exhibit. In addition to ongoing research and public policy efforts, Dr. Jagger collaborates with and is consulted by government agencies in the US and abroad, private industry, non-profit organizations, and academic institutions in the areas of safer medical device design and the prevention of healthcare-mediated exposures to bloodborne pathogens
The contribution of mortality and morbidity to smoking related differences in Disability Free Life Expectancy (DFLE) and Disability Life Expectancy (DLE) in Belgium
No full textBerger N., Nusselder W., Charafeddine R., Jagger C., Demarest S</p
Health expectancies in the European Union. The European Community Household Panel: data analysis
No full textREVES Paper No. 32
Calcium signalling mediated by the α9 acetylcholine receptor in a cochlear cell line from the Immortomouse
No full text1. We have investigated the characteristics of the alpha 9 acetylcholine receptor (alpha 9AChR) expressed in hair cell precursors in an immortalized cell line UB/OC-2 developed from the organ of Corti of the transgenic H-2Kb-tsA58 mouse (the Immortomouse) using both calcium imaging and whole-cell recording. 2. Ratiometric measurements of fura-2 fluorescence revealed an increase of intracellular calcium concentration in cells when challenged with 10 mu M ACh. The calcium increase was seen in 66 % of the cells grown at 39 degrees C in differentiated conditions. A smaller fraction (34 %) of cells grown at 33 degrees C in proliferative conditions responded. 3. Caffeine (10 mM) elevated cell calcium. In the absence of caffeine, the majority of imaged cells responded only once to ACh. A small proportion (<2 % of the total) responded with an increase in intracellular calcium to multiple ACh presentations. Pretreatment with caffeine inhibited all calcium responses to ACh. 4. In whole-cell tight-seal recordings 10 mu M ACh activated an inward, non-selective cation current. The reversal potential of the ACh-activated inward current was dependent on the extracellular calcium concentration with an estimated P(Ca)/P(Na) of 80 for the alpha 9 receptor at physiological calcium levels. 5. The data indicate that ACh activates a calcium-permeable channel alpha 9AChR in UB/OC-2 cells and that the channel has a significantly higher calcium permeability than other AChRs. The results indicate that the alpha 9AChR may be able to elevate intracellular calcium levels in hair cells both directly and via store release
The Meckel-Gruber syndrome protein TMEM67 controls basal body positioning and epithelial branching morphogenesis in mice via the non-canonical Wnt pathway.
No full textCiliopathies are a group of developmental disorders that manifest with multi-organ anomalies. Mutations in TMEM67 (MKS3) cause a range of human ciliopathies, including Meckel-Gruber and Joubert syndromes. In this study we describe multi-organ developmental abnormalities in the Tmem67(tm1Dgen/H1) knockout mouse that closely resemble those seen in Wnt5a and Ror2 knockout mice. These include pulmonary hypoplasia, ventricular septal defects, shortening of the body longitudinal axis, limb abnormalities, and cochlear hair cell stereociliary bundle orientation and basal body/kinocilium positioning defects. The basal body/kinocilium complex was often uncoupled from the hair bundle, suggesting aberrant basal body migration, although planar cell polarity and apical planar asymmetry in the organ of Corti were normal. TMEM67 (meckelin) is essential for phosphorylation of the non-canonical Wnt receptor ROR2 (receptor-tyrosine-kinase-like orphan receptor 2) upon stimulation with Wnt5a-conditioned medium. ROR2 also colocalises and interacts with TMEM67 at the ciliary transition zone. Additionally, the extracellular N-terminal domain of TMEM67 preferentially binds to Wnt5a in an in vitro binding assay. Cultured lungs of Tmem67 mutant mice failed to respond to stimulation of epithelial branching morphogenesis by Wnt5a. Wnt5a also inhibited both the Shh and canonical Wnt/β-catenin signalling pathways in wild-type embryonic lung. Pulmonary hypoplasia phenotypes, including loss of correct epithelial branching morphogenesis and cell polarity, were rescued by stimulating the non-canonical Wnt pathway downstream of the Wnt5a-TMEM67-ROR2 axis by activating RhoA. We propose that TMEM67 is a receptor that has a main role in non-canonical Wnt signalling, mediated by Wnt5a and ROR2, and normally represses Shh signalling. Downstream therapeutic targeting of the Wnt5a-TMEM67-ROR2 axis might, therefore, reduce or prevent pulmonary hypoplasia in ciliopathies and other congenital conditions
DEVELOPMENT PATHWAYS AND LAND MANAGEMENT IN UGANDA: CAUSES AND IMPLICATIONS
No full textThis paper investigates the patterns and determinants of change in livelihood strategies ("development pathways"), land management practices, resource and human welfare conditions in Uganda since 1990, based upon a community-level survey conducted in 107 villages. The pattern of agricultural development since 1990 involved increasing specialization and commercialization of economic activities, consistent with local comparative advantages and market liberalization. Six dominant development pathways emerged, all but one of which involved increasing specialization in already dominant activities: expansion of cereal production, expansion of banana and coffee production, non-farm development, expansion of horticultural production, expansion of cotton, and stable coffee production. Of these, expansion of banana and coffee production was most strongly associated with adoption of resource-conserving practices and improvements in resource conditions and welfare. Other strategies are needed for areas not suited for this pathway. Other factors also influenced land management and resource and welfare outcomes. Road development was associated with improvements in many welfare and some natural resource conditions, except forest and wetland availability. Irrigation was found to reduce pressure to expand cultivated area at the expense of forest and wetlands, and is associated with improvement in some welfare and resource indicators. Government and non-governmental organization programs were found to contribute to improvements in several resource and welfare indicators, though there were some mixed results. Such programs may cause declines in one area by focusing on improvements in another area. Thus, trade-offs appear to be inherent in many efforts to improve agriculture or protect resources. Population growth had an insignificant impact on most indicators of change, though there is some evidence of population-induced agricultural intensification. The findings support neither the pessimism of some neo-Malthusian observers or the optimism of some neo-Boserupian observers regarding the impacts of population growth.International Development, Land Economics/Use,
“Love Is Strong, and You're so Sweet” : JAGGER is essential for persistent synergid degeneration and polytubey block in arabidopsis thaliana
No full textSuccessful double fertilization and subsequent seed development in flowering plants requires the delivery of two sperm cells, transported by a pollen tube, into the embryo sac of an ovule. The embryo sac cells tightly control synergid cell death, and as a result the polyspermy block. Arabinogalactan proteins are highly glycosylated proteins thought to be involved in several steps of the reproductive process. We show that JAGGER, Arabinogalactan Protein 4, is an important molecule necessary to prevent the growth of multiple pollen tubes into one embryo sac in Arabidopsis thaliana. In jagger, an AGP4 knockout mutant, the pistils show impaired pollen tube blockage as a consequence of the survival of the persistent synergid. JAGGER seems to be involved in the signaling pathway that leads to a blockage of pollen tube attraction. Our results shed light on the mechanism responsible for preventing polyspermy in Arabidopsis and for safeguarding successful fertilization of all ovules in one pistil, ensuring seed set and the next generation
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