486 research outputs found
Evidence that inhibition of phorbol ester-induced superoxide anion formation by cyclosporin A in phagocytes is not mediated by direct inhibition of protein kinase C
No full textCyclosporin A (CsA) has been reported to inhibit phorbol myristate acetate (PMA)-induced superoxide anion (O2-) formation in human neutrophils and murine macrophages. We found that CsA inhibited O2- formation in HL-60 cells induced by PMA (30 nM) and phorbol dibutyrate (200 nM) with a half-maximal effect at 1 and 0.75 microM, respectively. One possible target of CsA action is protein kinase C (PKC) [EC 2.7.1.37] since phorbol esters activate this kinase. However, CsA did not inhibit PMA-mediated reduction of histamine-induced rises in cytosolic Ca2+ concentration in, and PMA-induced differentiation of, HL-60 cells and platelet aggregation. CsA did not reduce the activity of various recombinant c-PKC isoenzymes (alpha, beta 1 and gamma), n-PKC isoenzymes (delta and epsilon), an a-PKC isoenzyme (zeta) nor of PKC purified from rat brain in vitro. These data show that CsA inhibits phorbol ester-induced O2- formation in HL-60 cells but not other phorbol ester-mediated events and that inhibition by CsA of O2- formation cannot readily be attributed to direct PKC inhibition. We also show that CsA does not change the activity of nucleoside diphosphate kinase [EC 2.7.4.6] in HL-60 membranes nor the latter's physical properties
Optimal directed searches for continuous gravitational waves
No full textWide parameter space searches for long lived continuous gravitational wave signals are computationally limited. It is therefore critically important that available computational resources are used rationally. In this paper we consider directed searches, i.e. targets for which the sky position is known accurately but the frequency and spindown parameters are completely unknown. Given a list of such potential astrophysical targets, we therefore need to prioritize. On which target(s) should we spend scarce computing resources? What parameter space region in frequency and spindown should we search? Finally, what is the optimal search set-up that we should use? In this paper we present a general framework that allows to solve all three of these problems. This framework is based on maximizing the probability of making a detection subject to a constraint on the maximum available computational cost. We illustrate the method for a simplified problem
ChemInform Abstract: Convenient Preparation of Stereochemically Homogeneous 2-Acyl-3- sulfonyl-1,3-oxazolidines and Diastereoselective Grignard Additions to Form Protected Enantiopure 2-Hydroxyalkanals.
No full textLong survival in leigh syndrome: new cases and review of literature
No full textItem does not contain fulltextLeigh syndrome (MIM 25600), also known as infantile subacute necrotizing encephalomyelopathy, is a neurodegenerative disorder with characteristic bilateral symmetric lesions in basal ganglia and subcortical brain regions. It is commonly associated with systemic cytochrome c oxidase (COX) deficiency and mutations in the SURF1 gene (MIM 185620), encoding a putative assembly or maintenance factor of COX. The clinical course is dominated by neurodevelopmental regression, brain stem, and basal ganglia involvement (e.g., dystonia, apnea) with death often occurring before the age of 10 years. Herein, we present three sisters carrying a previously reported homozygous SURF1 mutation (c.868_869insT) that is predicted to result in a truncated protein with loss of function. Our patients show heterogeneous clinical findings with different distribution patterns of metabolic lesions in brain magnetic resonance imaging (MRI) as well as a Chiari malformation with hydrocephalus in one patient. However, all three siblings show an unusual long survival (12 years and > 16 years). COX activity was not detectable in one patient and strongly reduced in the other two. We discuss these findings with respect to a review of the literature. A total of 15 additional patients with survival > 14 years have been reported so far. Overall, no clear genotype-phenotype correlations are detectable among these patients
Erratum: Search for gravitational waves from compact binary coalescence in LIGO and Virgo data from S5 and VSR1 (Physical Review D - Particles, Fields, Gravitation and Cosmology)
No full textThis paper was published online on 5 November 2010 with an omission in the Collaboration author list. S. Dwyer has
been added as of 12 April 2012. The Collaboration author list is incorrect in the printed version of the journal
Erratum: All-sky search for gravitational-wave bursts in the first joint LIGO-GEO-Virgo run (Physical Review D - Particles, Fields, Gravitation and Cosmology (2010) 81 (102001))
No full textThis paper was published online on 5 May 2010 with an omission in the Collaboration author list. S. Dwyer has been
added as of 12 April 2012. The Collaboration author list is incorrect in the printed version of the journal
Publisher's Note: Search for gravitational waves from binary black hole inspiral, merger, and ringdown
No full textThis paper was published online on 6 June 2011 with an omission in the Collaboration author list. S. Dwyer has been
added as of 12 April 2012. The Collaboration author list is incorrect in the printed version of the journal
Merlin cluster - report for the BAR
No full textThis is the report to the BAR that contains the design specifications for the cluster that the GEO data analysis group at the AEI is planning to build in order to perform searches for continuous gravitational wave signals
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