3,703 research outputs found

    EVALUATION OF THE INFLUENCE OF PIG HAM POST – SLAUGHTERING REFRIGERATION ON HYGIENIC PARAMETERS SET IN REGULATION EC 2073/2005

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    In order to evaluate the influence of refrigeration on hygienic parameters, issued by EC Regulation n. 2073:2005 (amended by EC Regulation n. 1441:2007) for swine carcasses, 15 pig hams were tested for microbiological analysis, i.e. enumeration of microorganisms at 30°C and enumeration of enterobacteriacee. Ham swabbing was carried out at the end of slaughtering and after 24 hours of storing in refrigeration cells. The temperature-monitoring recorders were put in the hams at the end of cutting operations of carcasses, when the hams were placed in the refrigeration cells. The drop in the inner temperature of hams was monitored during the 24-hour storing time. In most cases, hams with an increase of background flora after 24 hours, had lower temperature at the beginning of refrigeration and the inner temperature need a shorter time to drop below 20°C, 10°C and 4°C, rather than hams associated with bacterial reduction. Therefore there was no correlation between dropping of temperature and bacterial load of hams, because the hygienic conditions of cutting operations prior to refrigeration have a greater influence on hygienic parameters than refrigeration alone

    Effects of different levels of dietary biotin on the performance and bone structure of broilers

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    We evaluated the effects of different levels of biotin on broilers performances and bone growth. Biotin was added at concentrations of 100, 200, 300, 400, 500 μg/Kg to a corn and soybean diet for yellow skin broiler production during the whole production cycle. Biotin at dosages of 200, 300, 400 μg/Kg increased growth rate, and, regardless of dosage, feed conversion rate in the second and third period of growth. Femur and tibiotarsus volume was slightly reduced while the bone mineral content of the same bones showed an increase as a result of biotin supplementation. Any particular dose-response effect was recorded with regard to plasma mineral content and ALP activity

    Comparative detection of Salmonella enterica in meat by 3M Molecular Detection Assay Salmonella and the ISO 6579:2002 method.

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    Introduction: Salmonella spp. is a zoonotic microorganism responsible for the second most common food-borne disease in the European Union. Purpose: The purpose of this study was the comparative detection of Salmonella enterica in meat products by the 3M Molecular Detection Assay Salmonella and the ISO 6579:2002 method. Methods: A total of 200 meat samples (raw meat from chicken, turkey, pork and beef, minced meats, sausages, offal and meat preparations) were tested. After pre-enrichment in BPW (37±1°C for 16-20 h) broth cultures were tested by: i) the 3M method employing a prototype machine; ii) the ISO 6579:2002 cultural method. Samples positive by the 3M method were subjected to a three-tube MPN enumeration method. Twenty-five grams per sample were diluted 1:10 in BPW. The three-tube MPN dilutions represented 1.0, 0.1, and 0.01 g of the samples. Results: Overall, 22 samples out of 200 (11.0%) were found positive for Salmonella spp. Fifteen samples were positive by both methods (68.2%); three more samples were found positive by the 3M method only and four by the ISO 6579 method only. One of the four samples testing positive by the ISO method only was found to be inhibited by the 3M method. Positive and negative results were available after 24 h using the 3M method while the ISO 6579 method required three days for negative results and five-seven days for positive ones. Salmonella enumeration ranged from <0.3MPN/g to 2.1 MPN/g. Significance: The 3M Molecular Detection Assay Salmonella is a time saving and easy to use method that can be very useful in processing food samples. Discordant results between the two methods could be attributable to the low level of contamination by Salmonella spp. in these naturally contaminated samples which implies stochastic randomness in the positive outcome of any low contamination sample regardless of the method used

    Evaluation of the disease course of Italian children with juvenile idiopathic arthritis treated with etanercept: preliminary results in 313 patients

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    Disease course of 313 Italian children with juvenile idiopathic arthritis treated with subcutaneous injections of etanercept is herein described
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