691 research outputs found

    An optimized method for in vitro propagation of African baobab (Adansonia digitata L.) using two-node segments

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    Adansonia digitata L. (African baobab), is an important multi-purpose tree, whose distribution is at present limited to wild or semi-domesticated individuals widespread in Africa. Its distribution is threatened by seedling clearance for other land use and potentially by overharvesting induced by growing commercial use of baobab fruit. Recently, efforts have been made to establish baobab domestication and conservation strategies, with mixed results due to the low germinability of baobab seeds, a factor that hinders the possibility of developing commercial A. digitata plantations. Here, micropropagation was tested as a method for clonal propagation of explants from in vivo-grown seedlings. In vitro shoot multiplication was achieved by enhanced axillary bud proliferation of sterilized two-node segments. Bud break was dependent on cytokinin supply, but the combination of 1.0 or 10.0 μM zeatin riboside and 10.0 μM indole-3-butyric acid (IBA) increased the formation of microshoots after 8 weeks of culture. Regenerated microshoots rooted successfully in in vitro nutrient medium containing 10.0 μM IBA and normally grew in a greenhouse after acclimatization

    Structure-activity relationships of N-phenyl-N’-benzothiazol-6-ylurea synthetic derivatives: cytokinin-like activity and adventitious rooting enhancement

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    Some years ago we demonstrated the cytokinin-like activity of the synthetic N-phenyl-N0-benzothiazol- 6-ylurea (PBU) and a relevant adventitious rooting adjuvant activity of symmetric urea derivatives devoid of any cytokinin- or auxin-like activity per se. Here we report the synthesis and the biological activity evaluation of nine symmetric or asymmetric ureas/thioureas, structurally related to PBU. None of them show cytokinin-like activity, while we demonstrate for the first time that PBU interacts with Arabidopsis cytokinin receptor CRE1/AHK4 in a heterologous bioassay system. Among the PBU derivatives, all the symmetric ureas/thioureas show an adventitious rooting adjuvant activity in various bioassays, confirming that this activity is strictly dependent on their chemical structure

    Draunara, installation, Federica Cellini, 2014

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     Draunara, Federica Cellini, Ana B.K, widewalls.ch , 26/05/2014  "Washed away onto the shores of the island, migrants from Africa keep arriving to Lampedusa, a small island just off the coast of Sicily. (...)  The Unstoppable Tempest  Draunara takes its name from the local term for a storm that swoops over the island coming from the sea. The author of the piece draws a parallel between the tempest and the overwhelming number of people arriving from the same direction. Although the arrivals ar..

    correction idelalisib exposure before allogenic stem cell transplantation in patients with follicular lymphoma an EBMT survey

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    The article “Idelalisib exposure before allogeneic stem cell transplantation in patients with follicular lymphoma: an EBMT survey”, written by Leopold Sellner, Johannes Schetelig, Linda Koster, Goda Choi, Didier Blaise, Dietrich Beelen, Fabrizio Carnevale Schianca, Jakob Passweg, Urs Schanz, Emmanuel Gyan, Federica Sora, Nicolaus Kröger, Gerald. G. Wulf, Gwendolyn Van Gorkom, Jiri Mayer, Corentin Orvain, Jean Henri Bourhis, Pavel Jindra, Victoria Potter, Francesco Zallio, Elisabeth Vandenberghe, Stephen Robinson, Patrick J. Hayden, Ibrahim Yakoub-Agha, Silvia Montoto, Peter Dreger, on behalf of the European Society for Blood and Marrow Transplantation Lymphoma and Chronic Malignancies Working Parties, was originally published Online First without Open Access. After publication in volume 55, issue 12, page 2335–2338, the author decided to opt for Open Choice and to make the article an Open Access publication. Therefore, the copyright of the article has been changed to © The Author(s) 2020 and the article is forthwith distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder

    Roles of 1,3-di(benzo[d]oxazol-5-yl)urea, an urea derivative, in carob and pine cuttings: adventitious rooting versus xylogenesis

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    Adventitious root (AR) formation is often a limiting step for vegetative plant propagation, specially forwoody species of economic value. There are evidences showing that adventitious rooting andxylogenesis, both auxin-mediated programs, may compete in different plant experimental systems, and the prevalence of xylogenesis vs rhizogenesis may negatively influence the results of a rooting protocol. Previous results demonstrate that two synthetic urea derivatives, named 1,3-di(benzo[d]oxazol-5-yl)urea (5-BDPU) and 1,3-di(benzo[d]oxazol-6-yl)urea (6-BDPU), combined with the auxin indole-3-butyric acid (IBA), enhance AR formation in cuttings of different woody species, e.g. Malus pumila (1) and Pinus radiata (pine; 2). Present research is the first attempt to utilize these two unusual chemicals for improving adventitious rooting in stem cuttings of Ceratonia siliqua L. (carob), a woody angiosperm of high environmental and commercial value (3), but with rooting recalcitrance. Another aim was to identify morphogenic roles of the most effective urea compound in common between carob and pine, to verify the persistence of the same actions in distant species. To the aims, firstly, different protocols were applied in carob, varying the time of chemicals application/dark exposition, in the presence/absence of different IBA concentrations, in order to stimulate AR formation. The results showed that 5-BDPU (10 μM), combined with IBA (1 μM), highly enhanced rooting in carob cuttings in comparison with IBA alone, better than 6-BDPU (10 μM), further reducing callus formation in comparison with IBA alone. Neither of the two urea derivatives showed rooting inductive ability per se, confirming previous results in pine (2) and that IBA was the central factor for AR induction in both species. However, the histological analyses on carob cuttings revealed a new morphogenic role for 5-BDPU, i.e., when used alone the compound favoured xylogenesis from the cambial cells, which were instead able to initiate the adventitious rooting process when also IBA was present. The histological analyses in pine hypocotyl cuttings confirmed that 5-BDPU alone (at the same concentration used in carob) favoured xylogenesis, starting from the AR-competent cells, which in this species were those bordering the resin ducts. In conclusion, in two distantly related species, this urea derivative exhibits a dual morphogenic role, being involved in the switching between rhizogenesis and xylogenesis depending on the presence/absence of exogenous auxin input. The results open the way for understanding xylogenesis as an alternative program inhibiting AR formation and successful micropropagation. 1) A. Ricci, M. Incerti, E. Rolli, P. Vicini, G. Morini, M. Comini, C. Branca (2006) Plant Growth Regul., 50, 201-209 2) F. Brunoni, E. Rolli, L. Dramis, M. Incerti, D. Abarca, A. Pizarro, C. Díaz-Sala, A. Ricci (2014) Plant Cell Tiss. Organ Cult., 118, 111-124 3) M. Öztürk, Ö. Seҫmen, S. Gucel, S. Sakcali (2012) Acta Hort., 964, 197-20

    Cooking pots from Alassa Pano Mantilaris and Paliotaverna

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    This final report on the 1984–2000 investigations at Alassa begins with the presentation of the rescue excavations of the settlement (Chapter 2) and tombs (Chapter 3) at Pano Mantilaris. This is followed by the account of the elite architecture and associated finds uncovered at Paliotaverna (Chapter 4) and a detailed description and discussion of the remarkable seal impressions found on many of the Alassa pithoi (Chapter 5). In-depth studies of the Alassa pithoi and all of the other pottery found at the site are presented in Chapters 6 and 7 by Priscilla Keswani and Ariane Jacobs, respectively. Federica Spagnoli presents a report on cooking pots in chapter 7. Reports by other specialists on a variety of topics may be found in the 10 appendices: the cylinder and stamp seals (Aruz), metallurgical finds (Kassianidou and Van-Brempt), marked pottery (Hirschfeld), C14 dates (Manning), human remains (Lorentz), faunal remains (Croft), coins (Destrooper), ground stone objects (Souter), and archaeometric studies of the pithoi (Nodarou) and other pottery (Jacobs et al.). The results from all of these studies are integrated within the conclusions that the author offers in Chapter 8 regarding the chronology and importance of Alassa within the broader cultural and sociopolitical context of LBA Cyprus

    Correction to: Impact of a mixed educational and semi-restrictive antimicrobial stewardship project in a large teaching hospital in Northern Italy (Infection, 10.1007/s15010-017-1063-7)

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    A technical error led to incorrect rendering of the author group in this article. The correct authorship is as follows: Daniele Roberto Giacobbe1, Valerio Del Bono1, Malgorzata Mikulska1, Giulia Gustinetti1, Anna Marchese2, Federica Mina3, Alessio Signori4, Andrea Orsi5, Fulvio Rudello6, Cristiano Alicino5, Beatrice Bonalumi3, Alessandra Morando7, Giancarlo Icardi5, Sabrina Beltramini3, Claudio Viscoli1; On behalf of the San Martino Antimicrobial Stewardship Group

    The Planning of Socialist Urbanity: The New City of Kukës in Albania.

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    This paper titled "The Planning of Socialist Urbanity: The New City of Kukës in Albania" has been submitted in March 2022 (abstract submission) to the “Ngā Pūtahitanga / Crossings: 39th Annual SAHANZ Conference and 16th Australasian UHPH Conference" hosted at the Te Pare School of Architecture and Planning, University of Auckland, Waipapa Taumata Rau, New Zealand, in 25-27 November 2022 (hybrid online/in situ format). This paper has been peer-reviewed and it has been accepted for publication in December 2022, and published in July 2023 in the Annual Conferences Proceedings of the Society of Architectural Historians, Australia and New Zealand (SAHANZ), “Ngā Pūtahitanga / Crossings”, Volume 39, edited by Julia Gatley and Elizabeth Aitken Rose, Auckland: SAHANZ 2023. ISBN: 978-0-646-88028-0 (ISSN 2653-4789 - online). More info on the SAHANZ/UHPH Joint Conference can be found here: https://www.sahanz.net/events/nga-putahitanga-crossings-a-joint-conference-of-sahanz-and-the-australasian-uhph-group/ Conference contributions are available online at the following link: https://www.sahanz.net/publications/annual-proceedings/?session=3709 The final paper written by Dr Pompejano can be also accessed and downloaded here: https://www.sahanz.net/wp-content/uploads/Pompejano_2022_SAHANZ.pdf The paper should be cited as follows: Federica Pompejano, “The Planning of Socialist Urbanity: The New City of Kukës in Albania.” In Proceedings of the Society of Architectural Historians, Australia and New Zealand: 39, Ngā Pūtahitanga / Crossings, ed. Julia Gatley and Elizabeth Aitken Rose, 379-87. Auckland: SAHANZ, 2023. Accepted for publication December 1, 2022. DOI: 10.55939/a5035pmg1tThis paper should be cited as follows: Federica Pompejano, "The Planning of Socialist Urbanity: The New City of Kukës in Albania." In Proceedings of the Society of Architectural Historians, Australia and New Zealand: 39, Ngā Pūtahitanga / Crossings, ed. Julia Gatley and Elizabeth Aitken Rose, 379-87. Auckland: SAHANZ, 2023. Accepted for publication December 1, 2022. DOI: 10.55939/a5035pmg1t Acknowledgement: This article originates from the scientific work conducted by the author during the implementation of the research project "Materializing Modernity – Socialist and Postsocialist Rural Legacy in Contemporary Albania (MaMo)" which has received funding from the European Union's Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie Grant Agreement No. 896925 (https://cordis.europa.eu/project/id/896925). This contribution reflects only the author's view, and the EU Commission is not responsible for any use that may be made of the information it contains

    1,3-di(benzo[d]oxazol-5-yl)urea acts as either adventitious rooting adjuvant or xylogenesis enhancer in carob and pine microcuttings depending on the presence/absence of exogenous indole-3-butyric acid

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    Ceratonia siliqua L. (carob) is a woody angiosperm of commercial value but, as often it happens with many tree species, its asexual propagation remains difficult because of adventitious rooting recalcitrance. In an attempt to improve the adventitious root (AR) formation in cuttings of this species, previous results obtained in Pinus radiata, a distantly-related woody gymnosperm, have been considered. In particular, adventitious rooting of pine hypocotyl cuttings has been enhanced by two urea derivatives, 1,3-di(benzo[d]oxazol-5-yl)urea (5-BDPU) and 1,3-di(benzo[d]oxazol-6-yl)urea (6-BDPU), combined with the auxin indole-3-butyric acid (IBA) (Brunoni et al., 2014, Plant Cell Tiss Organ Cult 118: 111). This research is the first attempt to utilize these two unusual chemicals, besides the auxin (IBA), to improve adventitious rooting in carob cuttings, with the aims to define their role in AR process and to identify morphogenic roles in carob, but also in pine, even when used alone. To the aims, results in carob were compared histologically with those obtained from the cuttings of pine. Different protocols were applied in order to enhance adventitious rooting in carob, using different chemicals application/dark exposition times and/or hormones (IBA, kinetin) combinations and concentrations. The results show that 5-BDPU (10 M) highly promoted AR formation in combination with IBA (1 M) when applied for 3 days, followed by a transfer onto hormone free medium (HF) up to culture end (four weeks). IBA was more effective to enhance AR-forming capability of the cuttings when applied alone than when combined with kinetin (Kin, 10 nM), a synthetic adenine derivative with cytokinin-like activity, which is frequently used to induce adventitious rooting in combination with this auxin (e.g., Della Rovere et al., 2013, Ann Bot 112: 1395). Kin alone and 5-BDPU alone were not AR-inductive. The histological analysis showed that the cambial cells initiated the ARs, and similar numbers of AR primordia were visible at day 12 after different AR-inductive treatments (i.e., IBA alone, IBA plus 5-BDPU, IBA plus Kin). No cutting treated with Kin alone, and rare HF (with or without 5-BDPU)-treated cuttings, showed AR primordia at the same day. The number of explants forming AR-primordia increased under IBA with 5-BDPU. By contrast, the cambial cells were stimulated to initiate deuteroxylem instead of ARs under the treatment with 5-BDPU alone. The histological analysis in pine microcuttings treated with IBA and/or 5-BDPU at the same concentrations confirmed that 5-BDPU applied alone enhanced xylogenesis, highlighting that this urea derivative exhibits a dual morphogenic role in both species, being involved in the switching between adventitious rooting and xylogenesis depending on the presence/absence of exogenous auxin
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