5,214 research outputs found
Contro la funzionalizzazione della contrattazione collettiva. Riflessioni sul pensiero di Mario Rusciano
No full textL'autore riflette sul pensiero di Mario Rusciano in punto di funzionalizzazione della contrattazione collettiva.The author reflects on the thought of Mario Rusciano in relation to the subject of the functionalisation of collective bargaining
SMLM datasets of the T cell receptor (TCR) on murine T cells on non-activating bilayers (label: CD3zeta-PS-CFP2 and H57-AF647)
No full text<p>Single molecule localization microscopy images recorded by Benedikt Rossboth (BR) and Florian Baumgart (FB) for Fig. 2b and Fig. 3b in Rossboth et al. 2018 Nature Immunology.</p><p>CD3zeta-PS-CFP2 PALM data: recorded 2016/11/21, 2016/11/15, 2016/09/28 by BR</p><p>H57-Alexa Fluor 647 dSTORM data: recorded on 2017/01/23 and 2017/01/24 by BR and FB</p><p>all recordings on fixed T-cells attached to non-activating bilayers (see publication for more details).</p><p>File format: SPE (TIFF). </p><p>Loading of files: </p><p>1. FIJI (supports SPE format)<br>2. Python: 3.1. pims.open or <br> 3.2. https://github.com/schuetzgroup/sdt-python (documentation: https://schuetzgroup.github.io/sdt-python/io.html)<br>3. Matlab: function can be provided upon request</p>
Il diritto penale nel canone di Mario Romano
No full textThis paper deals with the extension and the extraordinary scientific value of the works written by a great Master in Criminal Law, such as Mario Romano. The Author briefly presents some of the most relevant contributions given by Professor Mario Romano to the Criminal Science, first of all his "Commentario sistematico del codice penale" (Systematic Commentary on the penal code), a unique work. Finally, the paper talks about some topics which have been developed inside the work "Studi in onore di Mario Romano" (Studies in Honour of Mario Romano)
Introduzione, a Mario Tobino, Il Clandestino
No full textL'introduzione presenta il libro più ambizioso di Mario Tobino, Il Clandestino, dedicato al racconto della sua esperienza con i gruppi clandestini della Resistenza Viareggina, libro con cui l'autore vinse il Premio Strega, nel 1962, imponendosi all'attenzione del grande pubblico dopo il successo dei libri manicomiali. Una nuova edizione in cui Paola Italia valorizza i materiali inediti dell'Archivio Tobino conservato presso l'Archivio Contemporaneo A. Bonsanti del Gabinetto GP Vieusseux di Firenze,Paola Italia presents an Introduction to the new edtion of Il Clandestino, the most ambitious of Mario Tobino's novels, dedicated to the story of his experience with the groups of the Tuscan Resistance when he was a psichiatric doctor ar Lucca. With this book the author won the Strega Prize, in 1962, attracting the attention of a wide public after the success of his psichiatric books. A new edition where Paola Italia enhances the unpublished material of Tobino Archive preserved in A. Bonsanti Contemporary Archive of G.P. Vieusseux Cabinet of Florence
L'uno e il molteplice: su Catull. 5
No full textIn Catull. 5, the author cleverly works out two ancient topoi (‘let’s love, because the night/death is coming’; ‘kisses are the joy of lovers’), gaining a well balanced structure of the poem. In comparison with contemporary Graeco-Roman poetry (erotic epigrams – such as Philod. AP 9, 570 –, the Epitaph of Bion), both themes are handled with a remarkable taste for originality, especially in their ‘numerical’ aspects, in order to put special emphasis on the basic contrast between nox una and basia mille
In memoriam Mario Napoli
No full textL'autore ricorda la vita e le principali opere di Mario Napoli, suo Maestro e illustre studioso del Diritto italiano del lavoro.The author remembers the life and the most important works of Mario Napoli, his Master and one of the leading scholars of Italian labour law
La libertà da difendere
No full textIl contributo costituisce la prefazione del volume curato dallo stesso autore.The contribution is the introduction to the volume, whose editor is the same author
Figure data for Publication Trenzinger et al. in Lab Chip 2024
No full text<h2>Description of dataset</h2>
<p>This dataset includes all necessary information to reproduce the figures from the publication "<strong>Microdevice for confinement of T-cells on functionalized bio-interface</strong>".</p>
<div>DOI: <a title="Link to landing page via DOI" href="https://doi.org/10.1039/D5LC00248F">https://doi.org/10.1039/D5LC00248F</a></div>
<h3>Article: Microdevice for confinement of T-cells on functionalized bio-interfaces</h3>
<h3>Authors</h3>
<div><a href="https://pubs.rsc.org/en/results?searchtext=Author%3AChristoph%20Trenzinger">Christoph Trenzinger</a>,*<sup><em>a</em></sup> <a href="https://pubs.rsc.org/en/results?searchtext=Author%3ACaroline%20Kopittke">Caroline Kopittke</a>,<sup><em>a</em></sup> <a href="https://pubs.rsc.org/en/results?searchtext=Author%3ABarbora%20Kalouskov%C3%A1">Barbora Kalousková</a>,<sup><em>a</em></sup> <a href="https://pubs.rsc.org/en/results?searchtext=Author%3ANemanja%20%C5%A0ikani%C4%87">Nemanja Šikanić</a>,<sup><em>a</em></sup> <a href="https://pubs.rsc.org/en/results?searchtext=Author%3AMarina%20Bishara">Marina Bishara</a>,<sup><em>a</em></sup> <a href="https://pubs.rsc.org/en/results?searchtext=Author%3AGerhard%20J.%20Sch%C3%BCtz">Gerhard J. Schütz</a><sup><em>a</em></sup> and <a href="https://pubs.rsc.org/en/results?searchtext=Author%3AMario%20Brameshuber">Mario Brameshuber</a>*<sup><em>a</em></sup></div>
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<p>* Corresponding authors<br><sup>a</sup> Institute of Applied Physics, TU Wien, Wiedner Hauptstraße 8-10, 1040 Vienna, Austria</p>
<h3>Contact</h3>
<p><strong>E-mail:</strong> <a title="[email protected]" href="mailto:[email protected]">[email protected]</a>, <a title="[email protected]" href="mailto:[email protected]">[email protected]</a></p>
<h2>Technical details</h2>
<h3>Reproduction of the figures</h3>
<ul>
<li><strong>Python</strong>: for all figures generated with Python, the corresponding Jupyter Notebook file is provided. The <code>_readme.txt</code> contains information about the files in the corresponding folder, <code>requirements.txt</code> contains information about the Python version and dependencies.</li>
<li><strong>Matlab</strong>: the scripts to analyse data and to generate figures is provided in the corresponding folders. notes.txt files contain remarks for the analysis. All numerical data for analysis with Matlab is provided in CSV format.</li>
</ul>
<h3>Required Software</h3>
<ul>
<li>Matlab (Mathworks), tested with version 2019b</li>
<li>Cellprofiler (4.2.1), <a href="https://cellprofiler.org" target="_blank" rel="noopener">https://cellprofiler.org</a></li>
<li>Python (version 3.10), depencies are listed in the file <code>requirements.txt</code></li>
</ul>
<h3>Licenses</h3>
<p><em>Data is licensed under the <strong>Creative Commons Attribution 4.0 International</strong><br></em><em>Software is licensed under the <strong>MIT license</strong></em></p>
<h3>Further notes</h3>
<p>The <code>.cpproj</code> files are CellProfilerProject files, containing data in HDF5 format.<br>The <code>.czi</code> files are Carl Zeiss Imaging formats, and can be read e.g.with the <a href="https://www.openmicroscopy.org/bio-formats/">bio-formats</a> plugin for <a href="https://imagej.net/software/fiji/">ImageJ/Fiji</a>.</p>
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</div><p>Mechanical stimuli are an integral part of the natural cellular microenvironment, influencing cell growth, differentiation, and survival, particularly in mechanically challenging environments like tumors. These stimuli are also crucial in the T-cell microenvironment, where they play a role in antigen recognition and pathogen detection. To study T-cell mechanobiology effectively, <em>in vitro</em> methods must replicate these mechanical stimuli induced by compression, tension or shear flow, in the presence of antigen-presenting cells (APCs). While custom-made microdevices and microfluidic chips have successfully observed bulk cell behavior under mechanical strain, no existing device fully replicated the T-cell mechanoenvironment comprehensively. In this study, we developed a microdevice that integrates the mechanoenvironmental aspects of an APC mimicry with compression under live-cell imaging conditions. This device allows for precise confinement of cells between two glass surfaces, which can be individually coated with functional bio-interfaces. The microdevice is reusable and enables presetting of confinement heights, manual seeding of cells and the assembly of components directly at the microscope. To validate our microdevice we confined primary mouse T-cells on different APC-mimicking supported lipid bilayers while monitoring their morphology and migratory behaviour over time. To study the effect of confinement on TCR signalling, we tracked intracellular calcium levels and quantified Erk1/2 phosphorylation by immunostaining. We observed that T-cell morphology and motility are affected by confinement but also by bilayer composition. Moreover our findings suggest that confinement, despite not interfering with T-cell activation, might increase TCR background signalling in resting T-cells. Importantly, our microdevice is not limited to T-cell research; it can also serve as a platform for studying mechanical stimulation in other cell types, cell aggregates like spheroids and organoids, or even tissue samples in the presence of various bio-interfaces.</p>
La vida y pensamiento de Mario Alzamora Valdez
No full textIn the following lines, the author seeks to pay tribute and simultaneously indicate and show the significance of an illustrious jurist, the Dr. Mario Alzamora Valdez. The author firstly justifies the reason for emphasizing in Alzamora Valdez, then, take us back to the classes that Mario Alzamora Valdez received in the UNMSM and the “Universidad Católica”. To conclude, the author indicates the profiles of Mario Alzamora\u27s procedural thought as well as the location of his doctrines in the history of the Peruvian Procedural Law.En las siguientes líneas, el autor busca rendir un homenaje y a la vez señalar y mostrar el alcance de un ilustre jurista, el Dr. Mario Alzamora Valdez. El autor primeramente justifica el por qué de hacer hincapié en Alzamora Valdez, luego, nos remonta a las clases que recibió Mario Alzamora Valdez en la UNMSM y la Universidad Católica. Para concluir, el autor señala los perfiles del pensamiento procesal de Mario Alzamora así como la ubicación de sus doctrinas en la historia del Derecho Procesal Peruano
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