652 research outputs found
Gene loss and lineage specific restriction-modification systems associated with niche differentiation in the Campylobacter jejuni Sequence Type 403 clonal complex
No full textCampylobacter jejuni is a highly diverse species of bacteria commonly associated with infectious intestinal disease of humans and zoonotic carriage in poultry, cattle, pigs, and other animals. The species contains a large number of distinct clonal complexes that vary from host generalist lineages commonly found in poultry, livestock, and human disease cases to host-adapted specialized lineages primarily associated with livestock or poultry. Here, we present novel data on the ST403 clonal complex of C. jejuni, a lineage that has not been reported in avian hosts. Our data show that the lineage exhibits a distinctive pattern of intralineage recombination that is accompanied by the presence of lineage-specific restriction-modification systems. Furthermore, we show that the ST403 complex has undergone gene decay at a number of loci. Our data provide a putative link between the lack of association with avian hosts of C. jejuni ST403 and both gene gain and gene loss through nonsense mutations in coding sequences of genes, resulting in pseudogene formation
Die Römische Republik /
No full textWer sich für antike Geschichte interessiert, greift zu dieser knappen und gut lesbaren Geschichte der Römischen Republik, geschrieben von einem der bekanntesten Althistoriker Deutschlands. Jochen Bleicken (1926-2005) führt den Leser von der Zeit der Etrusker bis zum Ende der Republik, das die Herrschaft Caesars besiegelte. Alle wichtigen Phasen der republikanischen Geschichte entfalten sich: die Ständekämpfe, Aufstieg Roms zur Weltherrschaft - und die zugehörigen ungeheuren kriegerischen Auseinandersetzungen -, Ursachen und Beginn der inneren Krise seit den Gracchen, die Restauration unter Sulla und schließlich: die Auflösung der Republik und die Begründung der Monarchie. Aloys Winterling Jochen Bleicken, 1926-2005, war Professor für Alte Geschichte an der Universität Göttingen und u.a. Mitherausgeber von "Oldenbourg Grundriss der Geschichte" und der HISTORISCHEN ZEITSCHRIFT.Wer sich für antike Geschichte interessiert, greift zu dieser knappen und gut lesbaren Geschichte der Römischen Republik, geschrieben von einem der bekanntesten Althistoriker Deutschlands. Jochen Bleicken (1926-2005) führt den Leser von der Zeit der Etrusker bis zum Ende der Republik, das die Herrschaft Caesars besiegelte. Alle wichtigen Phasen der republikanischen Geschichte entfalten sich: die Ständekämpfe, Aufstieg Roms zur Weltherrschaft - und die zugehörigen ungeheuren kriegerischen Auseinandersetzungen -, Ursachen und Beginn der inneren Krise seit den Gracchen, die Restauration unter Sulla und schließlich: die Auflösung der Republik und die Begründung der Monarchie. Aloys Winterling Jochen Bleicken, 1926-2005, war Professor für Alte Geschichte an der Universität Göttingen und u.a. Mitherausgeber von "Oldenbourg Grundriss der Geschichte" und der HISTORISCHEN ZEITSCHRIFT.Mode of access: Internet via World Wide Web.Description based on online resource; title from PDF title page (publisher's Web site, viewed 08. Jul 2019
Essentials of services marketing / Jochen Wirtz, Christopher Lovelock.
No full textIncludes bibliographical references and indexes.(xxxii, 670 pages) :Essentials of Services Marketing, 3e, is meant for courses directed at undergraduate and polytechnic students, especially those heading for a career in the service sector, whether at the executive or management level. It delivers streamlined coverage of services marketing topics with an exciting global outlook with visual learning aids and clear language. It has been designed so that instructors can make selective use of chapters and cases to teach courses of different lengths and formats in either services marketing or services management
Bioprocess development and optimisation for 5-ketofructose production with gluconobacter oxydans at different scales
No full text5-Keto-D-fructose (5KF) was recently identified as a promising non-nutritive natural sweetener. Based on previously reported 5KF production process with Gluconobacter oxydans, 5KF production was further increased regarding titre, yield, and productivity, as a cost efficient production process is key to being competitive. The 5KF titre was increased to 545 g/L by feeding a highly concentrated fructose solution of 1200 g/L. An alternative feeding strategy was presented using solid fructose cubes, reaching the highest productivity above 10 g5KF/L/h. Moreover, the scale-up of the 5KF production to a 150 L pressurised fermenter was successfully demonstrated using liquid fructose solution. Synthetic media are preferred for industrial-scale fermentation. Therefore, a defined minimal medium for production of 5KF with G. oxydans was developed. A rich, defined synthetic medium, consisting of 45 components was used as a basis for medium development. By applying a systematic approach, a defined minimal medium called "Gluconobacter minimal medium"was developed containing inter alia amino acids isoleucine, glutamate and glycine and the vitamins nicotinic acid, pantothenic acid and p-aminobenzoic acid. Compared to commonly used media for Gluconobacter, similar results regarding cultivation time, yield and productivity were obtained. Plasmid-free strains are preferred for production of food additives. For plasmid-free 5KF production IK003.1::fdhSCL2 was developed. The 5KF production rate and yield obtained with this strain were considerably higher than for the single integration strains and approached the values of IK003.1 with plasmid-based fdhSCL expression. By the additional plasmid-based invertase integration, sucrose was used as a renewable and cheaper carbon source for 5KF production. Improving the cultivation conditions led to reduction of cultivation time by 40%, sucrose cleavage increased by 36% and OD600 increased 2.5 times. To achieve valuable information on profitability already at early stage of the process development, a process model was developed with SuperPro Designer® and used to determine the most effective process optimisation. The economy of scale showed the largest impact on 5KF production. The process optimisations conducted in this works successfully reduced the production cost by 14%. The production cost could potentially be further decreased by 20% if sucrose was used as the sole carbon source
Development and application of new small scale cultivation systems with online monitoring capabilities
No full textDevelopment and application of new small scale cultivation systems with online monitoring capabilities
No full textAnalysis, interpretation, and the local dimension of economic transformation: What went wrong and why?
No full textTransformation has been put in motion by a variety of both endogenous and exogenous forces. Although not any process was under the control of those countries, their choice of goals and instruments was anyway particularly great, at least theoretically. However, transformation was implemented as a rather narrowly defined and technically circumscribed problem-solving process aiming at applying sound general principles of economics and management to reach well-defined goals. It turned out to generate new problems and resulted in different outcomes in different countries and, within individual countries, in different territories. This paper treats transformation as innovation and considers that it had to deal with different dimensions, including both general principles and local features, opportunities, and constraints, and both analysis based on problem-solving, and interpretation of the new situation. These dimensions should have been managed simultaneously, but failed to do so. The paper provides a general explanation for the failure in managing simultaneously the various components of transformation and considers what the 2008 international crisis has revealed of the implementation of 20 years of transformation.Transformation, Local development, Reform, Analysis, Interpretation, Crisis, Washington Consensus, Innovation
Jochen Gerz // Jean Louis Garnell
No full textAlbertini's brief descriptions of Garnell's and Gerz's photo and text based works focus on the temporal and spatial limitations of language and image. Includes short texts by both artists
Materialist pedagogics – Hans-Jochen Gamm’s pedagogical approach to critical-humanist materialism
No full textDer Beitrag beschäftigt sich mit dem Ansatz des 2011 verstorbenen Erziehungswissenschaftlers Hans-Jochen Gamm. Kern dieses Ansatzes ist die materialistische Lesart der Gesellschaft und der in ihren Grundbedingungen aufgeworfenen Erziehungs- und Bildungsfragen. Rekonstruiert werden neben der spezifischen Materialismus-Interpretation Gamms auch die Anlage einer Erziehungs- und Bildungstheorie, die sich als Folge der Anwendung des materialistischen Erkenntnisverfahrens im pädagogischen Handlungsfeld konstituiert. Aus dieser Skizze ergeben sich Hinweise auf Anschlussmöglichkeiten, Theorieprobleme und Desiderata einer materialistischen Pädagogik. (DIPF/Orig.)The paper discusses the approach developed by the educational scientist Hans-Jochen Gamm, who died in 2011. The core of this approach is the materialist reading of society and of the educational issues explored with regard to their basic conditions. In addition to Gamm\u27s specific interpretation of materialism, the author reconstructs the outline of a theory of education which results from the application of a materialist method of cognition to the pedagogical field of action. Based on this outline, the author formulates possibilities of drawing on this approach as well as theoretical problems and desiderata of a materialist pedagogy. (DIPF/Orig.
A new stable splitting for singularly perturbed ODEs
No full textIn this publication, we consider IMEX methods applied to singularly perturbed ordinary differential equations. We introduce a new splitting into stiff and non-stiff parts that has a direct extension to systems of conservation laws and investigate its performance analytically and numerically. We show that this splitting can in some cases improve the order of convergence, demonstrating that the phenomenon of order reduction is not only a consequence of the method but also of the splitting.The authors would like to thank Sebastian Noelle for fruitful discussions. Furthermore, we would like to thank the anonymous referee for suggestions that helped improving the paper substantially. The second author has been partially supported by DFG project NO 361/3-3 (German Science Foundation)
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