1,721,166 research outputs found
Supplemental Material, sj-pdf-1-brn-10.1177_10998004211043571 - Maternal LINE-1 DNA Methylation in Early Spontaneous Preterm Birth
No full textSupplemental Material, sj-pdf-1-brn-10.1177_10998004211043571 for Maternal LINE-1 DNA Methylation in Early Spontaneous Preterm Birth by Anita Barišić, Aleksandra Stanković, Ljiljana Stojković, Nina Pereza, Saša Ostojić, Ana Peterlin, Borut Peterlin and Jadranka Vraneković in Biological Research For Nursing</p
Matrix metalloproteinases 1, 2, 3 and 9 functional single-nucleotide polymorphisms in idiopathic recurrent spontaneous abortion
No full textARTICLE
Matrix metalloproteinases 1, 2, 3 and 9
functional single-nucleotide polymorphisms
in idiopathic recurrent spontaneous abortion
Nina Pereza
a
, Sas
ˇ
a Ostojic
́
a,
*
, Marija Volk
b
, Miljenko Kapovic
́
a
,
Borut Peterlin
b
a
Department of Biology and Medical Genetics, Faculty of Medicine, University of Rijeka, B. Branchetta 20, 51000 Rijeka,
Croatia;
b
Institute of Medical Genetics, Department of Gynaecology and Obstetrics, UMC Ljubljana, 1000 Ljubljana,
Slovenia
*
Corresponding author.
E-mail address:
[email protected]
(S Ostojic
́).
Nina Pereza, MD, is a PhD student at the Faculty of Medicine, University of Rijeka. She works at the Department
of Biology and Medical Genetics and her main interest of research is reproductive genetics, especially genetic
causes associated with idiopathic recurrent spontaneous abortion.
Abstract
Idiopathic recurrent spontaneous abortion (IRSA) has been associated with abnormalities in the remodelling of endome-
trial extracellular matrix, as well as aberrant matrix metalloproteinase (MMP) gene expression in endometrium of IRSA women and
chorionic villi of IRSA concepti. This study investigated the association of five functional MMP gene promoter polymorphisms (MMP1
1607 1G/2G, MMP2
735 C/T, MMP2
1306 C/T, MMP3
1612 5A/6A and MMP9
1562 C/T) with IRSA. A total of 149 couples with
at least three consecutive IRSA and 149 fertile couples were included in a case–control study. Genotype analysis was performed
using PCR restriction fragment length polymorphism. Statistically significant differences were found in distributions of MMP2
735
CT (chi-squared 10.21,
P
= 0.006; OR 2.15, 95% CI 1.34–3.45,
P
= 0.001), and MMP9
1562 CC (chi-squared 9.06,
P
= 0.010; OR 2.21,
95% CI 1.30–3.80,
P
= 0.004) between IRSA women and controls. Combined analysis of MMP gene polymorphisms did not increase
their predictive value. There were no statistically significant differences in genotype and allele frequencies of any polymorphism
between IRSA men and controls. MMP2
735 C/T and MMP9
1562 C/T functional gene polymorphisms might be associated with
an increased risk of IRSA in women
The microtransplantation technique: a simple ad useful approach to study receptors transplanted into xenopus oocytes
Full text linkNeuroreceptors are involved in many neurological diseases and represent the preferential target for the pharmacological treatments. Thus functional studies of their activity, by the use of electrophysiological techniques, are a fundament approach to understand not only the pathological mechanism of many neurological diseases but also the mechanism of action of potential drugs. Unfortunately, this cannot be applied for studying the receptor activity in all the human tissues. The option is the use of animal models, however they often resemble only some of the neurological diseases in human. In addition, adult or old animals are not always suitable for electrophysiological studies of age-related diseases. Here, we propose the microtransplantation technique as a novel and useful method to study receptors in humans and, more in general, in adult animals
GT75 aptamer against eukaryotic elongation factor 1A as potential anticancer drug for castrate-resistant prostate cancer (CRPC).
Full text linkProstate cancer diagnosis is increasing, being the second most frequently cancer in men worldwide. The treatment of castrate-resistant prostate cancer is often unsuccessfully and new therapeutic interventions are searching for. Nucleic acid aptamers targeting eEF1A proteins are emerging molecular tools for the control of cancer growth. We found that an aptamer named GT75 was able to bind to eEF1A proteins of human prostate cancer cell lines and to significantly and specifically reduce their growth with respect to the control oligomer CT75. The highest anti-proliferation effect was found in the androgen-independent PC-3 cells. Interestingly, GT75 was able to specifically inhibit the migration of PC-3 cells but not that of the nontumorigenic PZHPV-7 cells. The overall results suggest that the GT75 aptamer targeting eEF1A proteins is a promising molecular drug to develop for the control of the castrate-resistant prostate cance
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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