1,318 research outputs found
User Preference on Artificial Reverberation and Delay Time Parameters
No full textAuthor P. D. Pestana was sponsored by national funds
through the Fundac¸ao para a Ci ˜ encia e a Tecnologia, Portu- ˆ
gal, in projects: “PEst-OE/EAT/UI0622/2014” and “PEstOE/MAT/UI2006/2014.”
Author J. D. Reiss is supported
by EPSRC Platform Grant: Digital Music, EP/K009559
Homeoprotein Hbx4 represses adhesion molecule governing cytokinesis and development
No full textHomeobox genes encode proteins with a highly conserved DNA-binding motif and provoke morphological diversification of body segments by differentially controlling the expression of downstream targets. Here, we have identified _hbx4_, one of many homeobox genes in _Dictyostelium discoideum_ and investigated its role during growth and development. In suspension, Hbx4-overexpressing cells, Hbx4^OE^, showed defects in cytokinesis and growth rate. During development, Hbx4^OE^ and _hbx4_-disrupting cells, _hbx4¯_ made differences in shape of mound and slug, cell-type proportioning from wild type KAx3 cells. These phenotypes were similar to those of mutant defective in _cadA_ encoding Ca^2+^-dependent cell adhesion molecule so that we investigated the relationship between _hbx4_ and _cadA_. Overexpression of Hbx4 inhibited the expression of _cadA_ and cAMP also failed to stimulate _cadA_ in Hbx4^OE^. Furthermore, gel mobility shift assay showed the promoter of _cadA_ contained Hbx4-binding site, indicating Hbx4 negatively regulates the expression of _cadA_. Proteome analysis revealed that overexpression of Hbx4 repressed the _rdiA_ and _abpB_ encoding rho guanine nucleotide dissociation inhibitor1, RhoGDI1 and actin bundling protein 34, ABP34, respectively. And the overexpression of _cadA_ in Hbx4^OE^ cells rescued the defects and increased mRNA level of _rdiA_, _abpB_ and one of Rho GTPase, _rac1b_. These results suggested that Hbx4 can modulate cytokinesis, cell sorting and cell-type proportioning by repressing _cadA_ that regulates GTPase-dependent signaling pathway
Limit Theorem in the space of continuous functions for the Dirichlet polynomial related with the Riemann zeta-funtion
No full text. A limit theorem in the space of continuous functions for the Dirichlet polynomial X mT d T (m) m oe T +it ; where d T (m) denote the coefficients of the Dirichlet series expansion of the function i T (s) in the half-plane oe ? 1, T = (2 \Gamma1 ln l T ) \Gamma1=2 , oe T = 1 2 + ln 2 l T l T and l T ? 0, l T ln T and l T !1 as T !1, is proved. Let s be a complex variable and i(s), as usual, denote the Riemann zetafunction. To study the distribution of values of the Riemann zeta-function the probabilistic methods can be used, and the obtained results usually are presented as the limit theorems of probability theory. The first theorems of this type were obtained in [1],[2], and they were proved in [3]-[5] using other methods. In modern terminology we can formulate it as follows. Let Manuscrit re¸cu le 20 octobre 1993 This paper has been written during the author's stay at the University Bordeaux I in the framework of the program "S&T Cooperation with Central and ..
Applying the method of fundamental solutions to harmonic problems with singular boundary conditions
No full textThe method of fundamental solutions (MFS) is known to produce highly accurate numerical results for elliptic boundary value problems (BVP) with smooth boundary conditions, posed in analytic domains. However, due to the analyticity of the shape
functions in its approximation basis, theMFS is usually disregarded when the boundary functions possess singularities. In this work we present a modification of the classical MFS which can be applied for the numerical solution of the Laplace BVP with Dirichlet boundary conditions exhibiting jump discontinuities. In particular, a set of harmonic functions with discontinuous boundary traces is added to the MFS basis. The accuracy of the proposed method is compared with the results form the classical MFS.The financial support from Center for Computational and Stochastic Mathematics (CEMAT–IST) through Fundac¸ ˜ao para a Ciˆencia e a Tecnologia (FCT) projects PEst-OE/MAT/UI0822/2014 and EXCL/MAT-NAN/0114/2012 is gratefully acknowledged
ASSESTMENT KESIAPAN DISTRITO OE-CUSSE UNTUK MENJALANKAN MUNIC�PIO/DESENTRALISASI
No full textDistrito Oe-Cusse is located in the middle territory ofRepublic of
Indonesia with a total area of 814.12 km2. Oe-Cussebelongs to administrative
areas of Democratic Republic of Timor Leste (RDTL). Based on the constitution
of RDTL in part I, article 5, paragraph 1,it is stated that the arrangement of the
region or the stateshall respect to the principle of decentralization of public
administration. On this basis, the government as a large organization should
follow up the constitution.
Municipio/decentralization is one of the most often-discussed issues and it
seems that Ministério da Administração Estatal e Ordenamento do Território
(MAEOT) is still busy to prepare to operate the municipio/desentralization. The
author has conducted research on the factors that prepared by Distrito Oe-Cusse in
order to run the municipio/decentralization. The goal is to determine what factors
are prepared and how is the readiness of Distrito Oe-Cusse to run the
municipality/decentralization.
The factors meant here are the apparatus resources, the infrastructure and
financial capacity of the area. While,the benefits of the research are to help the
author understand the science, as provision experience to increase the
professionalism and the research results are expected to benefit the academic
world, especially the issue of decentralization and also to contribute ideas for the
government of Timor-Leste in order to provide the authority and responsibility to
the regions. Through research, field observation and analysis which have been
done, it can be concluded that the readiness of the Distrito Oe-Cusse apparatus
and infrastructure resources are actually really ready. The indicators is that there
are INAP and SERA which is in charge of human resource development, there are
also 101 trainees in management field, there are 15 new buildings constructed,
there are budget for provision of facilities, there are existing activities to
improveand install telecommunication cables and there are new
generatorsavailable there and there are 14 employeesof electricity energy.
While the Distrito Oe-Cusse readiness from the side of financial capacity is not
really ready. Because there is no infrastructure meeting the standards, no laws
governing the protection and processing the potency of the region, there is no
authority and the rights of local governments to collect taxes and levies and the
levy of the Directorate/Department in the area paid to central government so that
there is no local genuine income (PAD) formed, and no budget financing for the
program of developingmunicipio / desentralizationfrom PAD.
Central and local government efforts are necessary to evaluate the
effectiveness of training and apparatus development. It is also necessary to build a
government office building which has good accessibility and quality, with
provision facilities, with the control price of phone bills. Besides, other things
needed are providing constant supervision and cooperation of central and local
area in the field of electric energy and establishing public infrastructure,
establishing laws to protect, managingthe potency of areas, providing authority
from the central government to local governments to levy taxes and charges and
continuing to improve and construct public infrastructure
Hormonal regulation of Gα i2 and mPRα in immortalized human oviductal cell line OE-E6/E7
No full textHeterotrimeric G proteins play a key role in membrane-mediated cell-signalling and hormonal regulation. Our earlier studies gave evidence of G protein subunit Gα i2 being under hormonal regulation in human in vivo. In this study, we used immortalized human oviduct epithelial cell line OE-E6/E7 as a model to study the hormonal regulation of Gα i2. We aimed at clarifying whether estradiol or progesterone could individually regulate the expression of Gα i2 and its potential signalling partners. Furthermore, we aimed to investigate which sex hormone receptors could potentially mediate the gene regulation in OE-E6/E7 cell line. OE-E6/E7 cells were cultured for 5 days with different concentrations of estradiol or progesterone. Quantitative real-time polymerase chain reaction (Q-PCR) was performed using cDNA of the hormone-treated cells to reveal any changes in gene expression. The presence of potential receptor targets in these cells was studied using PCR. Our data clearly showed that low concentrations of estradiol up-regulated the expression of Gα i2 gene and down-regulated the expression of membrane progesterone receptor mPRα gene in OE-E6/E7 cell line. Progesterone had no significant effect on Gα i2 gene expression, but it caused up-regulation of mPRα gene expression. In conclusion, it appears that sex hormones regulate the expression of Gα i2 and mPRα genes in a reverse manner in OE-E6/E7 cells. Our results suggest that estrogen receptor ERβ mediates the regulatory effects of estradiol in these cells. © The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.link_to_subscribed_fulltex
Mitochondrial functional state in clonal pancreatic beta-cells exposed to free fatty acids
No full textExcessive free fatty acid (FFA) exposure represents a potentially important diabetogenic condition that can impair insulin secretion from pancreatic beta-cells. Because mitochondrial oxidative phosphorylation is a main link between glucose metabolism and insulin secretion, in the present work we investigated the effects of the FFA oleate (OE) on mitochondrial function in the clonal pancreatic beta-cell line, MIN6. Both the long term (72 h) and short term (immediately after application) impact of OE exposure on beta-cells was investigated. After 72 h of exposure to OE (0.4 mm, 0.5% bovine serum albumin) cells were washed and permeabilized, and mitochondrial function (respiration, phosphorylation, membrane potential formation, production of reactive oxygen species) was measured in the absence or presence of OE. MIN6 cells exposed to OE for 72 h showed impaired glucose-stimulated insulin secretion and decreased cellular ATP. Mitochondria in OE-exposed cells retained normal functional characteristics in FFA-free medium; however, they were significantly more sensitive to the acute uncoupling effect of OE treatment. The mitochondria of OE-exposed cells displayed increased depolarization caused by acute OE treatment, which is attributable to the elevation in the FFA-transporting function of uncoupling protein 2 and the dicarboxylate carrier. These cells also had an increased production of reactive oxygen species in complex I of the mitochondrial respiratory chain that could be activated by FFA. A high level of reduction of respiratory complex I augmented acute FFA-induced uncoupling in a way compatible with activation of mitochondrial uncoupling protein by intramitochondrial superoxide. A stronger augmentation was observed in OE-exposed cells. Together, these events may underlie FFA-induced depression of the ATP/ADP ratio in beta-cells, which accounts for the defective glucose-stimulated insulin secretion associated with lipotoxicity.LR: 20061115; PUBM: Print-Electronic; DEP: 20030317; JID: 2985121R; 0 (Fatty Acids, Nonesterified); 0 (Reactive Oxygen Species); 2003/03/17 [aheadofprint]; ppublishSource type: Electronic(1
Reanalysis of OE hwæðer in the Left Periphery
No full textabstract: Despite the vast research on language carried out by the generative linguistics of Noam Chomsky and his followers since the 1950s, for theoretical reasons (mainly their attention to the mental abstraction of language structure rather than language as a performed product), historical linguistics from the start lay outside their research interest. This study is an attempt to bridge the gap between the formalism and theoretical constructs introduced by generative grammar, whose ultimate goal is to provide not only a description but also an explanation to linguistic phenomena, and historical linguistics, which studies the evolution of language over time. This main objective is met by providing a formal account of the changes hwæðer undergoes throughout the Old English (OE) period. This seemingly inconspicuous word presents itself as a case of particular investigative interest in that it reflects the different stages proclaimed by the theoretical assumptions implemented in the study, namely the economy principles responsible for what has become known as the CP cycle: the Head Preference Principle and the Late Merge Principle, whereby pronominal hwæðer would raise to the specifier position for topicalization purposes, then after frequent use in that position, it would be base-generated there under Late Merge, until later reanalysis as the head of the Complementizer Phrase (CP) under Head Preference. Thus, I set out to classify the diverse functions of OE hwæðer by identifying and analyzing all instances as recorded in the diachronic part of the Helsinki Corpus. Both quantitative and qualitative analyses of the data have rendered the following results: 1) a fully satisfactory functional and chronological classification has been obtained by analyzing the data under investigation following a formal theoretical approach; and 2) a step-by-step historical analysis proves to be indispensable for understanding how language works at the abstract level from a historical point of view. This project is part of a growing body of research on language change which attempts to describe and explain the evolution of certain words as these change in form and function.Dissertation/ThesisPh.D. English 201
Quantum detector tomography of a time-multiplexed superconducting nanowire single-photon detector at telecom wavelengths
No full textSuperconducting nanowire single-photon detectors (SNSPDs) are widely used in telecom wavelength optical quantum information science applications. Quantum detector tomography allows the positive-operator-valued measure (POVM) of a single-photon detector to be determined. We use an all-fiber telecom wavelength detector tomography test bed to measure detector characteristics with respect to photon flux and polarization, and hence determine the POVM. We study the SNSPD both as a binary detector and in an 8-bin, fiber based, Time-Multiplexed (TM) configuration at repetition rates up to 4 MHz. The corresponding POVMs provide an accurate picture of the photon number resolving capability of the TM-SNSPD
Simplified model of spectral absorption by non-algal particles and dissolved organic materials in aquatic environments
No full textAuthor Posting. © Optical Society of America, 2017. This article is posted here by permission of Optical Society of America for personal use, not for redistribution. The definitive version was published in Optics Express 25 (2017): 25486-25491, doi:10.1364/OE.25.025486.Absorption by non-algal particles (NAP, ad) and colored dissolved organic matter (CDOM, ag) are frequently modeled by exponential functions of wavelength, either separately or as a sum. We present a new representation of NAP-plus-CDOM absorption adg based on the stretched exponential function adg(λ) = A exp{−[s(λ − λo)]β}, whose parameter β can be considered a measure of optical heterogeneity. A double exponential representation of adg can be fit extremely well by a stretched exponential for all plausible parameter combinations, despite having one fewer free parameter than a double exponential. Fitting two published compilations of in situ adg data – one at low spectral resolution (n = 5, λ = 412–555 nm) and one at high spectral resolution (n = 201, λ = 300–700 nm) – the stretched exponential outperforms the single exponential, double exponential, and a power law. We thereby conclude that the stretched exponential is the preferred model for adg absorption in circumstances when NAP and CDOM cannot be separated, such as in remote sensing inversions.National Science Foundation (NSF) (OCE-1315201); National Science Foundation Graduate
Research Fellowship Program (NSF GRFP) (2388357); National Aeronautic and Space Administration
(NASA) Ocean Biology and Biogeochemistry program (NNX15AC08G)
- …
