65 research outputs found
Production of full length infectious cDNA clones of Strawberry mild yellow edge virus (SMYEV) and transmission trials with the strawberry aphid (Chaetosiphon fragaefolii)
No full text[EFF15S6] Session 7: Constanze Ruhm, Re-staging the Locations of Film History
No full text27 March 2015, 12:30, Birkbeck Cinema
Constanze Ruhm is an artist and author, whose work, exhibited internationally, encompasses installations, films and videos, publications and curatorial activities. Her artistic practice explores the interactions between film, film theory, theatrical forms, and new media, primarily with regard to questions of identity, representation and (feminist) film theory. Since 2006, she has been professor for Art and Media at the Academy of Fine Arts Vienna. Since 2007, she has also been an adjunct professor at the Art Institute Boston/Lesley University.
This event, combining screenings and discussion, was structured in two parts.
Part one – Constanze Ruhm in conversation with Roland-Francois Lack (UCL, creator of website: The Cine-Tourist) [Link: https://www.thecinetourist.net]
In La Difficulté d’une perspective: A Life of Renewal (2013), Constanze Ruhm, with Emilien Awada and using location research by Roland-Francois Lack, created this photographic series of eight locations from Une Femme est une femme (Jean-Luc Godard, 1961) shot from two different perspectives: first, as a precise replica of a shot from the original film, and then as a representation of the subjective perspective of the main female character (Angela/Anna Karina). The photos show what Anna Karina must have seen when she herself was seen by Godard and his camera.
Part two – screening and presentation
Film: Panoramis Paramount Paranormal (part of the Invisible Producers series), Constanze Ruhm with Emilien Awada, Austria, 2014, digital, 59 minutes, French and German with English subtitles
Out of their work on Une Femme est une femme, Ruhm and Awarda discovered the film studio St. Maurice, founded in 1913 and destroyed by fire in 1971. The film focuses on the history of this specific location and its place within the history of cinema. Subsequently, the apartment complex Le Panoramis was built on the same site
Production of full length infectious cDNA clones of Strawberry mild yellow edge virus (SMYEV) and transmission trials with the strawberry aphid (Chaetosiphon fragaefolii)
No full textConstanze Mozart Kajaanin Kaupunginteatterissa : Pukusuunnittelijan prosessi rokokoonäytelmän henkilöhahmon rakentamisessa
No full textOpinnäytetyön aiheena oli teatteripuvustuksen suunnittelu Kajaanin Kaupunginteatterin Amadeus-näytelmän yhdelle pääroolille, Wolfgang Amadeus Mozartin vaimolle Constanzelle. Amadeuksen ensi-ilta on 20.09.2014 teatteritalon näyttämöllä. Tekijän suunnittelema rokokootyylinen puvustus sisälsi seitsemän asukokonaisuutta, joista valmistettiin yksi kokonaisuus teknisine ohjeistuksineen. Opinnäytetyö toteutettiin tekijän pukusuunnittelu- ja ompelutaitoja hyödyntäen, sekä Pirkko Anttilan kriittis-realistisen evaluaation prosessimallia mukaillen. Suunnitteluprosessin tukena käytettiin J. Michael Gilletten teatterituotantoon tarkoitettua suunnittelu- ja ongelmanratkaisumallia. Lisäksi pukusuunnittelussa hyödynnettiin tekijän aikaisemmin suunnittelemia Amadeus-näytelmään sopivia printtikuvioita.
Tekijä perehtyi Amadeus-näytelmän käsikirjoitukseen ja 1700-luvun loppupuolen naisten pukeutumisen historiaan. Aineistoa hankittiin havainnoimalla yhtä teatteriesitystä ja kahta elokuvaa, jotka sijoittuvat rokokooaikaan. Havainnointiaineistoa täydensi näytelmän ohjaajan, skenografin ja opinnäytetyön tekijän pitämät kolme eri palaveria, joissa keskusteltiin näytelmän ideoista sekä pukuluonnoksista. Palaverit dokumentoitiin videoimalla. Pukuluonnoksista valittu ja tekijän valmistama asukokonaisuus kuvattiin puettuna Constanzen näyttelijälle. Tekijä sai työstään asiantuntijapalautetta ohjaajalta, skenografilta ja teatterin puvustossa työskenteleviltä ammattilaisilta palautelomakkeen avulla.The aim of the final project with thesis was to design theatrical costumes for one of the main characters, Wolfgang Amadeus Mozart’s wife Constanze, in the Amadeus Drama at Kajaani Town Theatre. The premiere of Amadeus is on 20 September 2014 on the stage of the theatre building. The Rococo costume set designed by the author includes seven outfits, of which one outfit with technical drawings was manufactured. The final project with thesis was implemented by exploiting the author’s skills in costume designing and sewing, together with adapting the principles of the critical-realistic evaluation process model presented by Pirkko Anttila. The design and problem solving concept of J. Michael Gillette, intended for theatre production, was used to support the design process. In addition, suitable print patterns designed previously for the Amadeus Play were also exploited in the costume designing.
The author became familiar with the script of Amadeus Drama and the history of women’s clothing in the late 18th century. The information was collected by observing one theatre show and two movies, which are placed in the Rococo time. The observation data was completed by three different meetings held by the director and the scenographer of the play and the author. The ideas and the costume sketches of the play were discussed in the meetings, which were documented by video. One outfit chosen from the sketches and produced by the author was worn by the actor playing the role of Constanze and photographed. The author received expert feedback about her work from the director, scenographer and the professionals of the theatre’s costume shop via the feedback blank
Diagnose- und Nachweisverfahren
No full text123 - Etablierung eines molekularen on-site Testverfahrens zur Diagnose von Phytoplasmen an Reben und ObstkulturenHolger Linck, Sven Keil, Frank Brändle, Annette Reineke
124 - Viren, Viroide und Phytoplasmen in Reben und Obstbäumen - Detektion von Mehrfachinfektionen mittels HochdurchsatzsequenzierungKerstin Zikeli, Constanze Berwarth, Dennis Knierim, Christoph Hoffmann, Michael Maixner, Stephan Winter, Wilhelm Jelkmann
125 - Vorkommen der Ulmenvergilbung in einheimischen Ulmenarten in DeutschlandBernd Schneider, Marlies Karaus, Heidrun Mattauch, Michael Kube
126 - Validierung des Ködertests mit Rhododendronblättern für ein routinemäßiges Monitoring von Probenmaterial auf Kontamination mit Phytophthora ramorum nach der EPPO-Richtlinie PM7/98 (2)Corina Junker, Sabine Werres
127 - Charakterisierung pilzlicher Schaderreger an Kamille (Matricaria recutita L.)Monika Götz, Katja Sommerfeld, Samad Ashrafi, Stefan Wagner, Ute Gärber
128 - In situ Immunfluoreszenz-Lokalisierung: Eine Methode zur einfachen Detektion von Beauveria spp. in BodenprobenCornelia I. Ullrich, Frank Rabenstein, Eckhard Koch, Natascha Heil, Marta Matek, Regina G. Kleespies
129 - Nachweis von Schildlausarten (Coccoidea) in BrandenburgUte Schönfeld123 - Development of an on-site molecular diagnostic tool for phytoplasmas in grapevine and other speacialty cropsHolger Linck, Sven Keil, Frank Brändle, Annette Reineke
124 - Application of next-generation sequencing for simultaneous detection of viruses, viroids and phytoplasmas in grapevine and fruit treesKerstin Zikeli, Constanze Berwarth, Dennis Knierim, Christoph Hoffmann, Michael Maixner, Stephan Winter, Wilhelm Jelkmann
125 - Occurrence of Elm Yellows Phytoplasma in native Elm Species in GermanyBernd Schneider, Marlies Karaus, Heidrun Mattauch, Michael Kube
126 - Validation of the bait test with rhododendron leaves for routine monitoring of samples for contamination with Phytophthora ramorum according to the EPPO Guideline PM7/98 (2)Corina Junker, Sabine Werres
127 - Characterisation of fungal pathogens of chamomille (Matricaria recutita L.)Monika Götz, Katja Sommerfeld, Samad Ashrafi, Stefan Wagner, Ute Gärber
128 - In situ immunofluorescence localization: A method for the rapid detection of Beauveria spp. in soil samplesCornelia I. Ullrich, Frank Rabenstein, Eckhard Koch, Natascha Heil, Marta Matek, Regina G. Kleespies
129 - Detection of scale species (Coccoidea) in BrandenburgUte Schönfel
First detection and complete genome characterization of a Cherry (C) strain isolate of plum pox virus from sour cherry (Prunus cerasus) in Germany
No full textAn isolate of plum pox virus (PPV) was detected in sour cherry (Prunus cerasus) in the Eastern region of Germany. Symptoms observed on infected sour cherry included leaf chlorosis, vein clearing, leaf abnormality and dark spots and rings on fruit. The complete genome sequence of this PPV isolate (GC27) was determined. The virus genome consists of 9795 nucleotides (nt), excluding the poly(A) tail at the 3′ terminus, with 5′ and 3′ untranslated regions (UTRs) of 146 and 217 nt, respectively. Phylogenetic analysis based on the complete genome, the nucleotide sequence of the coat protein and the deduced amino acid sequence of the PIPO protein indicate that PPV isolate GC27 is a member of the Cherry (C) strain of PPV. In pairwise comparisons, the complete genome (nt) of isolate GC27 is 98.2–99.7% identical to other C isolates; with much lower identities for isolates of other strains including approximately 83% identity for isolates of the strain Cherry Russian. This is the first detection of a strain C isolate of PPV in Germany
Transmission of Little cherry virus-1 (LChV-1) by Cuscuta europea to herbaceous host plants
No full textTransmission of Little cherry virus -1 (LChV-1) by Cuscuta europea to herbaceous host plants
No full textLittle cherry virus-1 (LChV-1) was transmitted from infected Prunus avium F12 rootstocks by Cuscuta europea to Nicotiana occidentalis ‘37B’. Transmission of the virus was confirmed by RT-PCR analysis of total nucleic acid extracts from dodder and N. occidentalis. Symptoms consisted of curled leaves, reddening of leaf margins and veins, and plant decline. In parallel attempts virus transmission was not successful for LChV-2. Propagation of LChV-1 by mechanical transmission on N. occidentalis failed, however the virus was transferred serially by grafting
Prevalence, genetic diversity, and molecular detection of the apple hammerhead viroid in Germany
No full textIntroduction: Apple hammerhead viroid (AHVd) is an emerging plant pathogen infecting apple orchards worldwide. Its genetic variability and geographical distribution remain poorly understood, limiting effective diagnostics and management strategies. Methods: In this study, 192 samples from German apple orchards were analyzed using reverse transcription (RT) and real-time PCR, one-step RT real-time PCR, and Sanger sequencing. Next-generation sequencing (NGS) was employed on pooled RNA extracts to explore genetic diversity. Phylogenetic relationships were inferred using maximum likelihood methods, and viroid-derived small RNAs (vd-sRNAs) were identified from small RNA sequencing data. Results and discussion: AHVd was detected in 78% of samples, with prevalence varying by region: southern (82%), eastern (90%), northern (72%), and western (70%) states of Germany. Phylogenetic analysis revealed distinct clusters linked to geographical origins, indicating isolated evolutionary pathways. NGS analysis uncovered 39% inter-sample variability and 169 polymorphic positions, while Sanger sequencing of RT real-time PCR products derived from the same samples showed only 3% variability, reflecting dominant quasispecies populations. Small RNA analysis mapped 128,388 reads to the AHVd genome, identifying hotspots within and outside the rod-like structure, suggesting structural and regulatory functions of vd-sRNAs. These findings underline AHVd’s genetic diversity. The complex relationship between AHVd genetic variability and symptom expression necessitates the development of highly sensitive diagnostic tools and adaptive management strategies to effectively monitor and control its spread in apple production
- …
