38,570 research outputs found
Recording of interview with Marinus van den Berg
No full textMarinus van den Berg is a Catholic pastor in Rotterdam; he was a student of Nouwen's in Utrecht and Yale.1 audio cassette (ca. 1 hr., 30 mins.)Title based on contents of the item. ; Located in audio cassettes box 16. ; The interviews are not transcribed. ; Digitized February 7, 2011.For more information please contact Special Collections, the University of St. Michael's College.Item consists of an interview (SR2009 02 105 93) with Marinus van den Berg conducted by Peter Naus in Rotterdam, The Netherlands on May 3, 2007
Supplementary Data for "Canine adrenomedullary and pheochromocytoma organoids: a novel in vitro model"
No full textSupplementary Data for "Canine adrenomedullary and pheochromocytoma organoids: a novel in vitro model".
van den Berg MF, Timmermans-Sprang EPM, Viets FC, van den Berg L, Danawar F, van Wolferen ME, Kooistra HS, Grinwis GCM, de Jong WHA, van Faassen M, Galac S.
Abstract
Background
Given the lack of effective medical treatment for pheochromocytomas (PCCs), a reliable in vitro model is needed to explore new therapies. Organoids are three-dimensional self-renewing structures that exhibit key features of their tissue of origin, providing valuable platforms for disease modeling and drug screening.
Aim
This study aimed to establish and characterize organoid cultures of canine normal adrenal medullas and PCCs.
Methods
Normal adrenal medullas from healthy dogs and tumor tissue from client-owned dogs with PCC were used to develop organoids. Primary cell suspensions were cultured in a 3D matrix, and organoids were established under optimized conditions. Organoids were characterized using histology, immunohistochemistry, immunofluorescence, qPCR, and metanephrine analysis by LC-MS/MS.
Results
Five adrenomedullary organoid lines were successfully established, demonstrating sustained growth. Organoid cultures were also derived from nine PCCs, although expansion was limited after passages 1-2. Both adrenomedullary and PCC organoids expressed differentiation markers (chromogranin A, synaptophysin, phenylethanolamine N-methyltransferase) and stem/progenitor markers (nestin, SOX10). Organoids retained key functional traits, as indicated by metanephrine levels in culture supernatants, which initially mirrored primary tumor patterns. A decline in both differentiation marker expression and metanephrine levels was observed over time, possibly due to organoid dedifferentiation or selective loss of differentiated chromaffin cells.
Conclusions
This study demonstrates the establishment of the first adrenomedullary and PCC organoid lines. While further optimization is needed, these organoids offer valuable potential as an in vitro model to investigate PCC pathophysiology and explore novel treatment strategies for this therapeutically challenging tumor
Invloed van de verwerkingsomstandigheden op de producteigenschappen van polyvinylchloride
No full textBij de fabricage van kunststofartikelen blijkt, dat een wisselende productkwaliteit optreedt, die sterk afhankelijk is van de, bij de fabricage gekozen, verwerkingsomstandigheden. Dit is in het bijzonder het geval bij artikelen van hard PVC, zoals onder andere buizen. Het doel van het onderzoek was inzicht te verkrijgen in de chemische en fysische processen, die aan dit verschijnsel ten grondslag liggen. Hiertoe is nagegaan, hoe een aantal eigenschappen worden beïnvloed door een behandeling op de mengwals gedurende verschillende tijden. Onderzocht werden: (A.) Chemische structuureigenschappen: 1. gemiddeld moleculairgewicht, 2. Huggins’ constante, 3. moleculairgewichtsverdeling, 4. vertakkingsgraad, 5. tacticiteit, 6. concentratie van radicalen in het walsvel, (B.) Fysische structuureigenschappen 1. specifiek volume 2.specifieke enthalpie, (C.) Producteigenschappen, 1. elasticiteitsmodulus 2. afschuivingsmodulus 3. rek bij breuk.Applied SciencesChemische WerkwijzenLaboratorium voor Chemische Technologi
A Mr. M. Hulsebos and P. Van Den Berg Have Written to the Editor of De Hope
No full textA Mr. M. Hulsebos and P. Van Den Berg have written to the editor of De Hope which appeared in the January 20 issue giving a description of the Amelia Colony which Rev. Albertus C. Van Raalte has promoted. The land is good, the climate is pleasant, and people are friendly. Anyone willing to work can make good progress there.https://digitalcommons.hope.edu/vrp_1860s/1492/thumbnail.jp
Argo 16
No full textIn juli 2011 is, in opdracht van Van der Wardt Bouw uit Eemnes, door Archeologenbureau Argo een archeologisch booronderzoek uitgevoerd op het plangebied aan de Weegbreestraat te Soest, gemeente Soest. De aanleiding tot het booronderzoek werd gevormd door geplande bodemverstorende werkzaamheden, welke een bedreiging zouden kunnen zijn voor eventueel aanwezige archeologische waarden. Naar verwachting konden zich binnen het plangebied resten uit de Vroege Steentijd tot de Moderne Tijd bevinden. Tijdens het booronderzoek zijn aanwijzingen gevonden voor een intact bodemprofiel vanaf ca. +4,20 tot +4,30 m NAP. Het maaiveld binnen het plangebied lag ten tijde van het onderzoek tussen ca. +5,00 en +5,50 m NAP. De bouwwerkzaamheden zullen volgens opgave van de opdrachtgever niet dieper dan 90 cm onder maaiveld reiken .
Geadviseerd wordt eventueel aanwezige archeologische lagen en sporen insitu
te bewaren, dit in lijn met het beleid van het Rijk, de provincie en de gemeente Soest. Dit kan relatief eenvoudig door ophoging van het hele terrein tot aan het maaiveldniveau van de omliggende percelen (ca. +5,50 m NAP). Op grond van onzekerheden die aan archeologisch booronderzoek kleven, kan echter niet volledig worden uitgesloten dat binnen het onderzochte gebied ook op hogergelegen niveaus nog archeologische resten voorkomen. Het verdient daarom aanbeveling om de uitvoerder van het grondwerk te wijzen op de wettelijke plicht archeologische vondsten te melden bij de bevoegde overheid
The Study of the Impact of Early Life Conditions on Later Life Events: A look across the individual's life course
No full textBerg, G.J. van den [Promotor
Van den Berg’s Essay on Muslim Clergy and the Ecclesiastical Goods in Java and Madura: A Translation
No full textPage range: 127-159A translation of a study by the Dutch scholar, L. W. C. van den Berg (1845-1927), which provides a detailed description of Islamic officialdom, mosques, religious practices, the administration of Islamic religious law, the relationship of religious staff to village administrators and residents, the administration and character of ecclesiastical property, and other aspects of Islam as it was practiced in Madura and Java, Indonesia, during the 1860s and 1870s
Pollen morphology of the genus Begonia in Africa
No full textThe morphology of the pollen grains of African Begonias is described, leading to the recognition of 15 pollen types. These pollen types are assumed to constitute natural units produced by evolution and the main purpose of this study has been to reconstruct the course of evolution and to apply the resulting insights to the various taxonomical problems. It has been attempted to formulate a critical approach to the problem of applying pollen-morphological data to phylogenetic reconstruction. The evidence from pollen morphology is compared with that from other disciplines, viz. seed morphology, the study of placentation types, karyology and, especially, macromorphological taxonomy. The relation between pollen morphology and geographical distribution is discussed, while the pollen morphology of Madagascan Begonias as well as of the allegedly related family of the Datiscaceae is also investigated
Natural control of Helicoverpa armigera in smallholder crops in East Africa
No full textThe African bollworm, Helicoverpa (=Heliothis) armigera , is one of the worst agricultural pests in Africa, attacking a variety of food and cash crops. For development of sustainable pest management, it is essential to study the ecology and natural mortality factors of the pest, and recently, the need for the assessment of the role of natural enemies, through life table studies, has been stressed in a number of workshops that focussed on the pest.Information available on the natural enemies of H.armigera, in Africa is reviewed, using published, unpublished and museum sources (Chapter 3). A large variety of natural enemies is represented in almost 300 host records, including 83 parasitoids identified to species and 93 identified only to genus. The taxonomy, distribution, biology, alternative hosts or prey, host plant associations and secondary natural enemies are detailed for all recorded natural enemies, and the different aspects are summarized and evaluated for the total natural enemy complex.During the three-year research programme reported here, H.armigera was studied in four crops commonly grown in smallholdings in Kenya: cotton, sunflower, maize and sorghum. The incidence of the pest varied widely between seven experimental sites in different agricultural zones of Kenya (Fig. 4.1). H. armigera , only occasionally reached damaging levels. A number of parasitoids was recorded, but their impact on H. armigera was generally low; Trichogrammatoidea spp. egg parasitoids and Linnaemya longirostris, a tachinid late-larval parasitoid, were the most common species. Two groups of predators were predominant throughout Kenya: Anthocoridae (mainly Orius spp.) and ants ( Pheidole spp., Myrmicaria spp. and Camponotus spp.), but their abundance fluctuated widely between sites (Fig. 4.3). Pathogens were scarce and did not play a significant role.In-depth studies on life tables and assessment of predation were conducted at Kakamega and Kibos, both in western Kenya. Oviposition of H. armigera coincided with early flowering of the crops (Fig. 5.4), except for cotton, where the period of oviposition was extended (Fig. 12.6). Life tables showed that immature mortality was generally high (82-99.3 %) on sunflower, maize and sorghum, but stage-specific mortality varied greatly between seasons (Tables 53-5.5). Mortality of young stages was highest in maize (Fig. 12.2). Key factor analysis demonstrated that predation-and-unknown-mortality of both of young and late developmental stages was the most important mortality factor (Fig. 12.3).Analysis of temporal and spatial association between numbers of pest stages and predators revealed that anthocorids are generally poorly associated with H.armigera, eggs on sunflower and sorghum (Chapter 6). This partly explains the relatively high survival of young stages on sunflower. On maize, the association of anthocorids with eggs and larvae of H.armigera, was stronger. Ants were better associated with H.armigera on sunflower than on maize or sorghum, which might be responsible for the relatively high late-larval mortality on sunflower.Parasitoids attacking this polyphagous pest are not equally common in different crops, but showed strong associations with particular crop species fed on by their host. In western Tanzania, a complex of three species attacked H.armigera, larvae predominantly on sorghum, whereas parasitism by two other species concentrated on cotton (Fig. 7. 1).Techniques to assess the apparent and irreplaceable mortality of H. armigera, due to predators, parasitoids and pathogens are discussed (Chapter 2). The role of predation and other, unknown mortality factors was studied in two crops where H. armigera , causes most damage: sunflower (Chapter 8) and cotton (Chapter 9). Mortality was measured from stage-frequency data on the pest, supplemented with data on recruitment of eggs onto plants, in plots with and plots without predators. Ants were excluded by banding every plant in a sub-plot with insect trap adhesive. Anthocorids were excluded by applying low concentrations of insecticide which killed the predators but not the pest.On sunflower, survival was higher than in previous trials. Exclusion of predators did not significantly affect survival of the pest (Table 8.2), however, anthocorids, which attack eggs but not larvae of H. armigera, appeared well after the oviposition peak of H. armigera. Moreover the density of ants was very low this particular season.On cotton, survival was extremely low; only 6 % reached the second larval stage (Fig. 12.9), and exclusion of predators did not increase survival. Thus unknown mortality factors were very important and masked the effect of predation. This background mortality appeared to be related to the poor hostplant condition, and the low number of feeding sites (fruiting parts) for larvae due to drought.To evaluate predators at greater pest densities, a series of predator exclusion cages and open control cages were inoculated with H. armigera eggs. Two weeks after inoculation, larval levels in the exclusion cages were 4 1/2 times greater than those in the control (Table 10.1), indicating that predators are capable to suppress pest numbers. To evaluate mortality during the egg stage, we exposed marked egg cohorts on plants. Within 48 h, anthocorids sucked 12-65 % of the eggs, an additional 15 % was lost and 6 % parasitized (Table 11.1).Implications of the findings for IPM, and areas for follow-up work are discussed (Chapter 12). Studies on intercropping cotton with maize or sorghum are promising and most feasible, because maize and sorghum may strongly affect natural enemy populations and pest infestation levels, while such methods stimulate sustainable agriculture in smallholdings. However, there will only be a brief period when the trap crop is attractive to ovipositing moths. A careful choice of varieties and planting dates might ensure the maximum effectiveness of trap crops in the case of sunflower. For cotton, where oviposition is extended over a period of three months (Chapter 9), planting of trap crops at regular intervals may be required, but a trap crop may be most crucial early in the season, because of its potential role to attract natural enemies into fields
De toekomst van het middenveld
No full textItem does not contain fulltextO. van Munster De toekomst van het middenveld Den Haag:Delwel Uitgeverij BV ,1998 90 6155 749
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