7,099 research outputs found
Risk factors for recurrence of deep infiltrating endometriosis after surgical treatment
No full textAim: To evaluate the frequency of complications and factors associated with the recurrence of endometriosis in women with deep infiltrating endometriosis (DIE) undergoing surgical treatment.
Methods: A retrospective observational cohort study with 72 women who underwent surgery and followed up by DIE at the University of Campinas from 2007 to 2017. The variables analyzed were clinical characteristics, use and type of drug treatment before and after surgery, operative time and complications inherent to the procedure, as well as the recurrence of lesions on imaging.
Results: The mean age of women was 39.7 ± 6.3 years and the mean follow-up was 4.56 ± 2.60 years. Complications were reported in 16.6% of surgeries and recurrence of lesions in 34.7%. The risk of intraoperative complications was higher in the presence of lesions of the bowel and in those who used intramuscular progestin before surgery. A higher risk of recurrence was observed among those who did not use hormonal treatment or used a levonorgestrel-releasing intrauterine device (LNG-IUD) in the postoperative period.
Conclusion: Women with DIE have a high rate of complications during surgical treatment and a higher risk of recurrence when they did not receive hormonal treatment or when treated with LNG-IUD after surgery
Cl-IB-MECA enhances TNF-α release in peritoneal macrophages stimulated with LPS.
No full textAdenosine receptor A3 (A3R) belongs to the Gi/Gq-coupled receptor family, that leads to the intracellular cAMP reduction and intracellular calcium increase, respectively. A3R is widely expressed and it can play a crucial role in many patho-physiological conditions, including inflammation. Here we investigate the effect of Cl-IB-MECA, A3R agonist, on the production of TNF-α. We found that Cl-IB-MECA enhances LPS-induced TNF-α release in peritoneal macrophages. This effect is reduced by MRS1191, A3R antagonist and by forskolin, activator of adenylyl cyclase. pIκBα increased in LPS+Cl-IB-MECA-treated macrophages, while total IκB kinase-β (IKKβ) reduced. Indeed, p65NF-κB nuclear translocation increased in cells treated with LPS+Cl-IB-MECA. Moreover, IMD 0354, IKKβ inhibitor, significantly abrogated the effect of Cl-IB-MECA on TNF-α release. Inhibition of protein kinase C (PKC) significantly reduced Cl-IB-MECA-induced TNF-α release in LPS-stimulated macrophages. Furthermore, LY-294002, PI3K inhibitor, reduced the TNF-α production enhanced by Cl-IB-MECA, although the phosphorylation status of Akt did not change in cells treated with LPS+Cl-IB-MECA than LPS alone. In summary, these data show that Cl-IB-MECA is able to enhance TNF-α production in LPS-treated macrophages in an NF-κB- dependent manner
Order-disorder, Polytypes and Twinning in the Crystal Structure of vurroite
No full textVurroite, ideally Pb20Sn2(Bi,As)22S54Cl6, is a complex mineral type, where the two minor chemical components, Sn and Cl, act as essential constituents together with Pb, Bi, As and S 1, 2. X-ray single crystal data on vurroite strongly indicate an orthorhombic Fcentred symmetry 1, 3. In this study the crystal structure of vurroite is interpreted as an OD structure belonging to the category III of OD structures composed of equivalent layers 4. The application of the OD procedures allowed the derivation of the OD-groupoid family (λ and σ operations), as well as the MDO (Maximum Degree of Order) structures. The layer symmetry (λ) is A(2)mm, the interlayer symmetry (σ) consists of a glide plane n1/2,1/2 and two-fold screw axes parallel to 010 and 001 with the translation components 1⁄4 b and 1⁄4 c, respectively. For this OD family two MDO polytypes exists. The former has monoclinic symmetry, C12/c1, whereas the latter is monoclinic, P12/c1. The OD treatment of the crystal structure of vurroite allowed to prove that the true symmetry of this mineral is monoclinic and that the apparent orthorhombic symmetry observed for the X-ray pattern of the measured crystal is due to a twinning phenomenon. Prof. S. Merlino is gratefully acknowledged for his contribution on the OD interpretation of the structure of vurroite. 1 Pinto D., PhD thesis, University of Bari, 2004, 165. 2 Garavelli A.,Mozgova N.N., Orlandi P., Bonaccorsi E., Pinto D., Borodaev Y., Canadian Mineralogist, 2005, in press. 3 Pinto D., Balić-Žunić T., Bonaccorsi E., Makovicky E., 26th Nordic Geological Winter Meeting, Uppsala, Sweden,2004, 106-107, abstract. 4 Dornberger-Schiff K., Abh. Dtsch. Akad. Wiss. Berlin. Kl. Chem. Geol. Biol., 1964, 3, 1-107
Role Of The Different Sexuality Domains On The Sexual Function Of Women With Premature Ovarian Failure.
No full textWomen with premature ovarian failure (POF) often manifest complaints involving different aspects of sexual function (SF), regardless of using hormone therapy. SF involves a complex interaction between physical, psychological, and sociocultural aspects. There are doubts about the impact of different complaints on the global context of SF of women with POF. To evaluate the percentage of influence of each of the sexuality domains on the SF in women with POF. Cross-sectional study with 80 women with POF, matched by age to 80 women with normal gonadal function. We evaluated SF through the Female Sexual Function Index (FSFI), a comparison between the POF and control groups using the Mann-Whitney test. Component exploratory factor analysis was used to assess the proportional influence of each domain on the composition of the overall SF for women in the POF group. SF was evaluated using FSFI. Exploratory Factor Analysis for components was used to evaluate the role of each domain on the SF of women with POF. The FSFI score was significantly worse for women with POF, with a decrease in arousal, lubrication, orgasm, satisfaction, and dyspareunia. Exploratory factor analysis of SF showed that the domain with greater influence in the SF was arousal, followed by desire, together accounting for 41% of the FSFI. The domains with less influence were dyspareunia and lubrication, which together accounted for 25% of the FSFI. Women with POF have impaired SF, determined mainly by changes in arousal and desire. Aspects related to lubrication and dyspareunia complaints have lower determination coefficient in SF. These results are important in adapting the approach of sexual disorders in this group of women. Benetti-Pinto CL, Soares PM, Giraldo HPD, and Yela DA. Role of the different sexuality domains on the sexual function of women with premature ovarian failure. J Sex Med 2015;12:685-689.12685-
Gyrinus (Oreogyrinus) rhyssonotum Colpani & Benetti & Hamada 2019, sp. n.
No full textGyrinus (Oreogyrinus) rhyssonotum sp. n. (Figs 1 A–E, 2A–B, 3A–C) Type locality. BRAZIL, Amazonas state, Barcelos County, Serra do Aracá, 00°54'03.89"N 63°25'45.30"W (Figs 3 A–C). Type material. Holotype ♂ (INPA, pinned) “ BRAZIL, Amazonas State, Barcelos County, Serra do Aracá, first-order stream, 00°54'03.89"N 63°25'45.30"W, 1104 m, 27.VII.2009, leg. Pes, A.M.O. &. Hamada, N.”. Paratypes (29 exs): 2 ♂, 8 ♀ (1 ♂, 7 ♀ INPA, pinned; 1 ♂, 1 ♀ ZSM, pinned), same locality as holotype; 2 ♂ (INPA, pinned), same locality except “pond near Igarapé da Anta, 30.VII.09, leg. Pes, A.M.O. &. Hamada, N.”; 10 ♂, 7 ♀ (INPA, alcohol) same data except “stream, Igarapé da Anta, 00°54'30.38"N 63°26'24.32"W, 116 m, 01.VIII.09, leg. Pes, A.M.O. &. Hamada, N.”. Diagnosis. Pronotal and elytral margins broad (Fig. 1A); elytral disc lacking reticulation, appearing polished; elytral striae VII–IX sulcate, striae I–XI with mostly distinct round punctures in longitudinal series; elytral apex round to slightly truncate (Fig. 1A); epipleural angle indistinct; median lobe of aedeagus shorter than parameres, broad, with apex broadened and almost truncate, base 1.4 times broader than apex (Fig. 2A); parameres broad with apex truncate, with slightly prominent internal angle; gonocoxae elongate, with apices truncate (Fig. 2B). Description. Male. Habitus. Medium-sized species. Body oval, broad, weakly convex in lateral view, greatest convexity near elytral mid-length, evenly depressed anteriorly and posteriorly (Fig. 1A). Measurements (n = 20). TL: 4.44–4.95 mm; MW: 2.53–2.80 mm; HL: 0.74–0.95 mm; HW: 1.52–1.73 mm; CL: 0.12–0.17 mm; CW: 0.66–0.76 mm; DD: 0.50–0.59 mm; LW: 0.45–0.63 mm; PL: 0.75–0.81 mm; EL: 3.23– 3.72 mm; EH: 1.25–1.67 mm; EW: 1.23–2.80 mm; PTL: 0.80–0.96 mm; PFL: 1.07–1.21 mm; PFW: 0.29–0.33 mm; PTAL: 0.45–0.50 mm; PTAW: 0.15–0.19 mm. Coloration (Figs. 1 A–C). Head, pronotum and elytra black, with blue metallic reflections (Fig. 1A, B); lateral margins of pronotum and elytra (striae VIII–XI) with reflections bronzy yellow and green. Ventral view dark brown, with bronzy metallic reflections; labial palpomeres and last maxillary palpomere with dark-brown apices; meso and metacoxae darker with orangish-yellow pitches apically; abdominal ventrites VII and VIII reddish brown to dark orange yellow; legs dark yellow to orangish-yellow with dark spots. Sculpture and structure (at a magnification of 120×). Head. 2.0 times wider than long (Fig. 1B); front with two round depressions; frontolateral margins convex, reticulation evident, composed of scale-like sculpticells; vertex and frons with striae evident and sparse punctures separated by 2– 4X diameter of a puncture, frontolateral and postocular margins wrinkled (Fig. 1B); clypeus 1.2 times longer than labrum, with anterior edge nearly straight, frontoclypeal suture with posterior margin sinuous, lateral margins slightly convex (Fig. 1B), reticulation evident, composed of scale-like sculpticells, striae evident throughout the clypeus, punctures sparse, separated by 2– 4X diameter of a puncture; labrum convex, punctation strongly impressed on anterior margin, separated by 1.5–2.0X diameter of a puncture. Thorax. Pronotum. Anterior margin sinuous, posterior margin slightly curved, lateral margins broad and wrinkled (Figs 1A,B); pronotal transverse line strongly impressed with well marked wrinkles anteriorly and posteriorly (Fig. 1 B–D); pronotal disc with well impressed wrinkles and dense micropunctures (Figs 1C, D); punctures present throughout the pronotum, strongly impressed, separated by 1.5–2.0X their diameter (Fig. 1D). Elytra. Striae VII–IX sulcate, intervals I–III with linear disposition basally, intervals I–VI convex; stria XI strictly marginal; elytral margin uniform over almost all of its length, narrowing near epipleural apical angle (Fig. 1A); lateral margin separated from apex by a plica; fine punctation present among elytral intervals I–XI, distance between the nearest punctures 1.5–2.0X diameter of a puncture (Fig. 1E); large punctures of elytral series well marked (Fig. 1E); elytral apex rounded to slightly truncate; epipleural angle slightly distinct, sutural angle distinct (Fig. 1A). Mesoventrite grooved on lateral margin, apex with anterior margin rounded, groove evident in medial portion, extending to the posterior portion; medial pit present; setae present on anterolateral region. Profemur 3.7X longer than maximum width. Protarsus dorsally convex, broad, 2.6–3.0X longer than wide, about 1/ 3X length of protibia. Abdomen. Tergite VIII wider than long, apex rounded, with light yellow setae present on the entire surface, with weak longitudinal carina, ending before reaching apex. Aedeagus (Fig. 2A). median lobe of aedeagus shorter than parameres, broad, in dorsal view with lateral margins sinuous, weakly attenuated at the end of basal 2/3 and then broadened in apical 1/3 towards apex, which is slightly triangular and weakly raised medially; width of median lobe about 0.8 times width of apex of parameres; base 1.4 times broader than apex; in lateral view median lobe thick, dorsally curved. Parameres broad, with apex strongly truncate and slightly prominent internal angle. Female. Sculpture (micropunctures and wrinkles) more evident than in male. Elytral disc free of reticulation, elytral intervals I–XI with fine punctation. Gonocoxae (Fig. 2B) elongate, straight, narrowed at middle, with external and internal margins slightly concave, apex truncate, slightly concave; base wider than apex. Etymology. The specific epithet rhyssonotum refers to the quite evident wrinkles on the pronotum; rhysso (G.) = wrinkled; notum (G.) = the back). Distribution. This species is currently known only from the Barcelos county, Amazonas State, in northern Brazil (Fig. 3 A–C). Habitat. Specimens of this species were collected in streams and pools (Fig. 3 B–C).Published as part of Colpani, Daniara, Benetti, Cesar João & Hamada, Neusa, 2019, Gyrinus rhyssonotum sp. n., a new species of Gyrinidae (Coleoptera: Adephaga) from northern Brazil, pp. 164-170 in Zootaxa 4560 (1) on pages 165-168, DOI: 10.11646/zootaxa.4560.1.9, http://zenodo.org/record/262750
Semblanza del Dr. Henrique Benaím Pinto
No full textLa Cátedra de Clínica y Terapéutica A del Hospital Universitario de Caracas, conserva vigen- tes muchas de las enseñanzas del Dr. Benaím Pinto, quien fuera su Jefe por muchos años, y lo aquí expuesto es un pequeño homenaje a quien la Universidad Central de Venezuela y la medicina interna venezolana tanto debemos. El Dr. Benaím Pinto, nació en Caracas el 02 de agosto de 1922, descendiente de una familia de inmigrantes judíos sefarditas, constituida por Elías Benaím Pinto, padre, y Estrella Pinto Pilo, madre, siendo el segundo de 6 hijos, 3 de los cuáles fueron médicos. Su educación primaria la inició en el Colegio Paraíso de Caracas y a los 8 años se mudó a París donde permaneció por 2 años. Regresó a Caracas en 1932 y continuó sus estudios en el Colegio San Pablo, graduándose de bachiller a los 17 años en 1939 con un promedio de 20 puntos
Lucabindiite, (K,NH4)As4O6(Cl,Br), a new fumarole mineral from the “La Fossa” crater at Vulcano, Aeolian Islands, Italy
No full textLucabindiite, ideally (K,NH4)As4O6(Cl,Br), is a new mineral found as a medium-temperature fumarole encrustation (T = 170 °C) at “La Fossa” crater of Vulcano, Aeolian Islands, Italy. The mineral deposited as aggregates of micrometer-sized hexagonal and platy crystals on the surface of the pyroclastic breccia in association with arsenolite, sal ammoniac, sulfur, and amorphous arsenic-rich sulfurite. The new mineral is colorless to white, transparent, non-fluorescent, has a vitreous luster and a white streak. The calculated density is 3.68 g/cm3. Lucabindiite is hexagonal, space group P6/mmm, with a = 5.2386(7) Å, c = 9.014(2) Å, V = 214.23(7) Å3, and Z = 1. The eight strongest reflections in the X-ray powder-diffraction data [d in Å (I) (hkl)] are: 3.20 (100) (102), 2.62 (67) (110), 4.51 (52) (002), 4.54 (30) (100), 1.97 (28) (113), 1.49 (21) (115), 1.60 (21) (212), 2.26 (19) (112). Lucabindiite’s average chemical composition is (wt%): K2O 5.14, As2O3 84.71, Cl 3.63, Br 6.92, F 0.77, (NH4)2O 2.73, O=F,Cl,Br –1.84, total 102.06. The empirical chemical formula, calculated on the basis of 7 anions pfu, is [K0.51(NH4)0.49]Σ1.00 As4.00O5.93(Cl0.48Br0.40F0.19)Σ1.07. According to chemical analyses and X-ray data, lucabindiite is the natural analog of synthetic phases with general formula MAs4O6X where M = K, NH4 and × = Cl, Br, I. The crystal structure is characterized by neutral As2O3 sheets arranged parallel to (001). The As atoms of two neighboring sheets point at each other and the sheets are separated by interlayer M (=K, NH4) and × (=Cl, Br, F) atoms. The name is in honor of Luca Bindi (b. 1971), Professor of Mineralogy and former Head of the Division of Mineralogy of the Natural History Museum of the University of Florence. Both the mineral and the mineral name have been approved by the IMA-CNMNC Commission (IMA 2011-010)
Cl-IB-MECA enhances TRAIL-induced apoptosis via the modulation of NF-kappaB signalling pathway in thyroid cancer cells.
No full textApoptosis is an endogenous process that can be a useful anti-cancer tool. This study aimed to investigate the effect of Cl-IB-MECA, adenosine receptor A3 agonist, on TRAIL-induced apoptosis of thyroid carcinoma cells. Cl-IB-MECA enhanced TRAIL-mediated apoptosis in FRO but not in ARO cells. This effect was correlated to higher expression levels of DR5 on FRO than ARO cells, that instead presented higher levels of decoy receptors, DcR1 and DcR2. To understand the cross-talk between the effect of Cl-IB-MECA and TRAIL, we evaluated the nuclear translocation of p65 and c-Rel. Since the dependency by NF-kappaB, TRAIL promoted the nuclear translocation of both p65 and c-Rel subunits. However, the addition of Cl-IB-MECA led to the predominant translocation of c-Rel after TRAIL addition. Furthermore, Bcl-2, cFLIP and pAkt were lower induced than caspase-3 and -9 in FRO cells. To discriminate a specific effect of TRAIL, we used tumour necrosis factor-alpha (TNF-alpha) with Cl-IB-MECA. In this case, no synergism was observed. In addition, the effect of Cl-IB-MECA was not A3 receptor-dependent since its antagonists, MRS1191 and FA385, failed to block Cl-IB-MECA activity on TRAIL-treated FRO cells. In conclusion, Cl-IB-MECA enhanced TRAIL-mediated apoptosis via NF-kappaB/c-Rel activation and DR5-dependent manner. This study may shed light on a potential drug cocktail that may prove useful as anti-cancer in an in vivo animal model
Adoptive Immunotherapy with Cl-IB-MECA-Treated CD8+ T Cells Reduces Melanoma Growth in Mice.
No full textCl-IB-MECA is a selective A3 adenosine receptor agonist, which plays a crucial role in limiting tumor progression. In mice, Cl-IB-MECA administration enhances the anti-tumor T cell-mediated response. However, little is known about the activity of Cl-IB-MECA on CD8+ T cells. The aim of this study was to investigate the effect of ex vivo Cl-IB-MECA treatment of CD8+ T cells, adoptively transferred in melanoma-bearing mice. Adoptive transfer of Cl-IB-MECA-treated CD8+ T cells or a single administration of Cl-IB-MECA (20 ng/mouse) inhibited tumor growth compared with the control group and significantly improved mouse survival. This was associated with the release of Th1-type cytokines and a greater influx of mature Langerin+ dendritic cells (LCs) into the tumor microenvironment. CD8+ T cells treated with Cl-IB-MECA released TNF-α which plays a critical role in the therapeutic efficacy of these cells when injected to mice. Indeed, neutralization of TNF-α by a specific monoclonal Ab significantly blocked the anti-tumor activity of Cl-IB-MECA-treated T cells. This was due to the reduction in levels of cytotoxic cytokines and the presence of fewer LCs. In conclusion, these studies reveal that ex vivo treatment with Cl-IB-MECA improves CD8+ T cell adoptive immunotherapy for melanoma in a TNF-α-dependent manner
Rare sulfosalts from Vulcano, Aeolian Islands, Italy. VII. Cl-bearing galenobismutite
No full textCl-bearing galenobismutite (with Cl ranging from 0.12 to 1.45 wt.%), discovered among high-temperature fumarole incrustations at "La Fossa" crater, Island of Vulcano, Italy, has been chemically and structurally investigated. The mineral is commonly associated with bismuthinite, cannizzarite, lillianite, heyrovskýite and galena, and rarely with kirkiite, vurroite and other less well characterized Pb(Bi)-sulfochlorides. Electron-microprobe investigations show that Cl-bearing galenobismutite from Volcano has a wide compositional field (Pb:Bi atomic ratio ranging from 0.52 to 0.72). The statistical analysis of chemical data shows correlations between the main elements and chlorine, positive Pb versus Cl and negative Bi versus Cl, according to the coupled heterovalent substitution scheme: Pb2+ + Cl- ⇌ Bi3+ + S2-. The compositional variation of Cl-bearing galenobismutite can be represented by the general empirical formula Pb1+xBi2-x (S4-x-yClxSey), with 0 4σ(Fo)] and 1.49% for 376 reflections [Fo > 4σ(Fo)], respectively. The structure of Cl-bearing galenobismutite is topologically identical to that of Cl-free galenobismutite. The excess of Pb observed in Cl-bearing galenobismutite is incorporated in the trigonal prismatic M2gb and M3gb sites. No Pb-for-Bi substitution was observed at the octahedral position M1gb. We do not find evidence of a preferential distribution of Cl among the anion positions
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