12,771 research outputs found
The particulate methane monooxygenase from Methylococcus capsulatus (Bath)
No full textThe isolation procedure for the pMMO complex has been optimised to obtain a high
specific activity enzyme from Methylococcus capsulatus (Bath). The enzyme is
comprised of the pMMO hydroxylase (pMMOH) consisting of polypeptides 47,26 and
23kDa molecular mass. In addition to this, a putative pMMO reductase (pMMOR) was
also found to be necessary to maintain propylene oxidising activity. This component
was found to consist of two polypeptides of approximately 63 and 8kDa. Preliminary Nterminal
sequence data of the large subunit ofpMMOR indicates that the sequence bears
70% similarity to the methanol dehydrogenase (MDH) from Methylococcus capsulatus
(Bath). Therefore, we tentatively propose that the" MDH can act as a reductase
component to the pMMOH.
The significance of this result prompted investigations into the previous published
proposals that electrons derived from the methanol oxidation reaction can be channelled
back into the methane oxidation reaction by the methanol dehydrogenase, independent
of NADH. Any effect of methanol to act as a reductant to pMMO in membrane
preparations was lost upon isolation of the pMMO complex, indicating the necessity to
maintain a fully functional methanol dehydrogenase (MDH) upon isolation. In addition
to this, the in vitro electron donors of pMMO, NADH and duroquinol were found to act
via distinct pathways of electron transfer (electron transport inhibitor studies).
Electron paramagnetic resonance (EPR) spectroscopy data provided evidence that the
copper in the active site of pMMO existed as a mononuclear copper (II) centre not a
trinuclear copper centre suggested by Chan and coworkers (Chan et al., 1993; Nguyen et
al., 1994, 1996a, 1996b, 1998). In addition to this preliminary data also indicates the
presence of an iron centre which is only EPR visible after reduction of the complex
suggesting the majority of iron in the complex is EPR invisible. The exact nature of this
iron centre is still unclear.
A structural study of the pMMO complex has also been undertaken using electron
microscopy studies in conjunction with single particle analysis. This allowed low
resolution projection maps of different views of the pMMO complex to be generated.
The complex appears to exist in a polymeric state of at least a dimer, possibly a tetramer
if the molecular weight analysis calculated by sedimentation equilibrium analysis is
taken into account.
This study has provided some insight into basic characteristics and the structure of a
duroquinol-driven pMMO complex and its interaction with other electron transfer
proteins
Simplicity and subject
No full textEssay in catalogue to accompany the 'William Scott: Simplicity and Subject' exhibition held at the Victoria Art Gallery, Bath between 7 September and 17 November 2013
Letter From William Bell Scott to Mr Chambers
No full textabstract: Concerning Scott's thanks, his writings about his own works, and a manuscript of "The Nightingale Unheard."Seller's Description: Reads "A.L.S. from Author to Mr. Chambers explaining how busy he is... The sonnet is printed in the book. Fredeman: 56.7 £87.50"Handwritten Note: Unknown handwriting at top right reads "June 1st 1877."Publication Details: "The Nightingale Unheard" published in "Poems" by William Bell Scott.Creation Date Details: Undated range is the author's lifespan.Provenance: Removed from:
Poems / by William Bell Scott. Ballads, studies from nature, sonnets, etc. / illustrated by seventeen etchings by the author and L. Alma Tadema. Publisher London : Longmans, Green, 1875. CALL #
HAYDEN SPECIAL COLL SPEC PRB-13
Bath: Topographic Views
No full textBath Abbey, view from the south, depicting York Street in the foreground; Famous for mineral springs discovered by Romans in the first century and revived in the 18th century; captured by Saxons 577; abbey was built in the 10th century; was important wool center by the 12th century; noted for Royal Cresent (1767-1775), Prior Park (1735-1743) by Robert Adam and for its Roman ruins. Source: Thesaurus of Geographic Names (Notes) [website]; http://www.getty.edu/research/conducting_research/vocabularies/tgn/ (accessed 2/7/2008
Scott Belsky in Conversation with DesignByThem - Part One.mp4
No full textWatch here as Scott Belsky (CEO/Founder Behance + Author Making Ideas Happen) joins Sydney design duo Sarah Gibson and Nicholas Karlovasitis from DesignByThem to discuss some of the challenges facing creative companies as they try to grow their business. In part one Nick and Sarah get some great advice from Scott about what makes an effective partnership and how to learn from the challenges that can arise. Scott also explains the Behance technique of ?Concepting Teams? with representatives from all areas of the business to brainstorm issues or new ideas
Cardozo AELJ Author Interview Series: Scott Semaya, Class of 2023
No full textThe Cardozo AELJ Author Interview Series seeks to give our readers further insight into the Articles and Notes published in the Cardozo Arts & Entertainment Law Journal. In this interview, Scott Semaya discusses his Note, Name, Image and Likeness: Giving College Athletes the Clearest Guidance to Best Profit off Their NIL, which was published in Volume 41, Issue 2.
This post was originally published on the Cardozo Arts & Entertainment Law Journal website on June 6, 2023. The original post can be accessed via the Archived Link button above
Cardozo AELJ Author Interview Series: Scott Semaya, Class of 2023
No full textThe Cardozo AELJ Author Interview Series seeks to give our readers further insight into the Articles and Notes published in the Cardozo Arts & Entertainment Law Journal. In this interview, Scott Semaya discusses his Note, Name, Image and Likeness: Giving College Athletes the Clearest Guidance to Best Profit off Their NIL, which was published in Volume 41, Issue 2.
This post was originally published on the Cardozo Arts & Entertainment Law Journal website on June 6, 2023. The original post can be accessed via the Archived Link button above
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Cardozo AELJ Author Interview Series: Scott Semaya, Class of 2023
No full textThe Cardozo AELJ Author Interview Series seeks to give our readers further insight into the Articles and Notes published in the Cardozo Arts & Entertainment Law Journal. In this interview, Scott Semaya discusses his Note, Name, Image and Likeness: Giving College Athletes the Clearest Guidance to Best Profit off Their NIL, which was published in Volume 41, Issue 2.
This post was originally published on the Cardozo Arts & Entertainment Law Journal website on June 6, 2023. The original post can be accessed via the Archived Link button above
Scott Belsky in Conversation with DesignByThem - Part Two.mp4
No full textSarah Gibson and Nicholas Karlovasitis from DesignByThem continue their conversation with Scott Belsky (CEO/Founder Behance + Author Making Ideas Happen). They discuss the relationship between taking financial risks in creative enterprise whilst valuing professional standards in their work
Dataset for Continuous Production of Cellulose Microbeads by Rotary Jet Atomization
No full textContinuous Production of Cellulose Microbeads by Rotary Jet Atomization. Data which concerns the production of cellulose microbeads using ionic liquids, co-solvents and jet breakup. This data reports on the continuous fabrication of cellulose microbeads in the size range 20-500 μm with narrow size distribution, via rotary jet atomization. Utilizing Rayleigh breakup as a method of droplet generation, jets of cellulose dissolved in solutions of [EMIm][OAc] and DMSO are expelled from rotating nozzle systems, generating droplets which, when captured in a suitable anti-solvent bath, initiate phase-inversion allowing regeneration of the cellulose into beads. The dataset includes calculations of dimensionless parameters as well as droplet sizes and distributions.This data was gathered using particle size analysis (Mastersizer 3000). Beads were produced using cellulose dissolved in ionic liquids, and subjected to pressure and rotational atomization. Further details may be found in the associated paper
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