1,366,775 research outputs found
OneWorld : Find volunteers and set up volunteering events
No full textVolunteering is a cornerstone of our society. We always tend to think on a much wider perspective hence the reason why people agree to work without pay by choice. Therefore, working for a non-profit organization that benefits society is appealing to us.
The purpose of this thesis is to develop a concept which would make the volun-teering process easier. It’s commonly known that people often have a strong de-sire to volunteer yet never proceed further because the process of finding a cause your passionate about and joining that cause can be a tedious task.
As a solution to this problem, An Android application will be developed so that it can be used to find volunteering events as well as create your own. These events will be easily accessible and simple to register to. The application will be devel-oped using Java and Android studio will be my IDE and Firebase will serve as my server
cGAS/STING/TBK1/IRF3 innate immunity pathway can regulate chromosomal instability in a p21-dependent manner
No full textChromosomal instability (CIN) in cancer cells has been reported to activate the cGAS-STING innate immunity pathway via micronuclei formation, thus affecting tumor immunity and tumor progression. However, converse effects of the cGAS/STING pathway per se on CIN have not yet been investigated. I addressed this issue using knockdown and add-back of each component of the cGAS/STING/TBK1/IRF3 pathway, and monitored the extent of CIN by measuring micronuclei formation after release from nocodazole-induced mitotic arrest. Interestingly, knockdown of cGAS (cyclic GMP-AMP synthase) along with induction of mitotic arrest in HeLa and U2OS cancer cells clearly resulted in an increased micronucleus phenotype and chromosome missegregation. Knockdown of STING (stimulator of interferon genes), TBK1 (TANK binding kinase-1), or IRF3 (interferon regulatory factor-3) also increased micronuclei formation. Moreover, transfection of cGAMP, the product of cGAS enzymatic activity, as well as add-back of cGAS WT (but not catalytic-dead mutant cGAS), or WT or constitutively active STING (but not an inactive STING mutant) rescued the micronuclei phenotype, demonstrating that all components of the cGAS/STING/TBK1/IRF3 pathway play a role in preventing CIN. Moreover, p21 levels were decreased in cGAS-, STING-, TBK1- and IRF3-knockdown cells in association with precocious G2/M transition and an enhanced micronuclei phenotype. Overexpression of p21 or inhibition of CDK1 in cGAS-depleted cells reduced micronuclei formation and abrogated precocious G2/M transition, indicating that the decrease in p21 and subsequent precocious G2/M transition is the main mechanism underlying the induction of CIN by defective cGAS/STING signaling.I. INTRODUCTION 1
II. MATERIALS AND METHODS 14
A. Antibodies 14
B. Cell culture 14
C. Synchronization and drug treatment 15
D. Plasmids and transfection experiments 16
E. Knock-down experiments 16
F. Immunoblotting 17
G. Immunocytochemistry 18
H. Time-lapse analysis 18
I. Mitotic index 19
J. Statistical analysis 19
III. RESULTS 20
A. Investigating cGAS/STING pathway to choose best suited cancer cell-line for my experimental setting 20
B. cGAS add-back can rescue cells from cGAS-depletion induced CIN 22
C. cGAS down-regulation augments chromosomal missegregations 26
D. Micronuclei formation is regulated by enzymatic activity of cGAS 29
E. STING plays mediating role to maintain chromosomal stability 32
F. Depletion of TBK1 or IRF3 also enhanced CIN phenotypes 35
G. p21 expression levels are dependent on cGAS/STING pathway 37
H. p21 as a principal regulator in maintaining chromosomal integrity via cGAS/STING pathway 42
IV. DISCUSSION 47
V. CONCLUSION 52
REFERENCES 53Maste
A Novel DNA-PK/PLK1/STING/IRF3 Axis Regulates Normal Mitotic Progression
No full textDoctor1. INTRODUCTION 1
2. MATERIALS AND METHODS 10
2.1. Cell culture 10
2.2. Antibodies 10
2.3. Synchronization and drug treatment 11
2.4. Plasmids and transfection experiments 12
2.5. Knock-down experiments 12
2.6. Immunoblotting 14
2.7. cGAMP ELISA 15
2.8. Native-PAGE 15
2.9. Time-lapse analysis 16
2.10. 2D Blue Native-PAGE 16
2.11. Immunocytochemistry 17
2.12. Immunoprecipitation 17
2.13. In-vitro kinase assay 18
2.14. Quantitative RT-PCR 19
2.15. Mitotic index 19
2.16. Statistical analysis 20
3. RESULTS 21
3.1. IRF3 is phosphorylated during mitosis in a cGAS-independent but STING-dependent manner 21
3.2. Phosphorylation of IRF3 is required for normal mitotic progression 28
3.3. STING and IRF3 dimerization is a pre-requisite during mitosis 35
3.4. DNA-PKcs activity is required for STING and IRF3 phosphorylation during mitosis 39
3.5. PLK1 is the major mitotic kinase responsible for IRF3 phosphorylation during mitosis 47
3.6. STING, IRF3, DNA-PK and PLK1 form a complex during mitosis where STING acts as a mediator 53
3.7. Recruitment of PLK1 and IRF3 to the DNA-PK/STING complex requires DNA-PK activity 62
3.8. Ku70 and Ku80 may play different roles in the activation of STING/IRF3 axis in a cell-dependent manner 66
4. DISCUSSION 71
5. REFERENCES 7
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Optimal utilization of frequency ancillary services in modern power systems
No full textThe widespread global adoption of wind energy sources has established a significant presence in the existing power grid. However, the massive integration of intermittent wind energy poses forecasting errors, prompting the need for supplementary reserves from conventional energy sources with increased operational expenses and carbon emissions. Hence, to facilitate the seamless operation of large-scale wind-integrated power grids, it is imperative to harness the potential of renewable energy sources and leverage flexible loads to deliver power-balancing services. In this research, dynamic real-time power dispatch strategies have been developed for the Automatic Generation Control (AGC) system to integrate the reserve capacities of conventional generation units and wind power plants and utilize the demand response capabilities of flexible loads for power balancing services. A comprehensive power system grid model was developed in DigSilent PowerFactory software, consisting of coal-based energy systems, wind energy systems, gas turbines, and cold storage units as flexible loads. The study is divided into different case studies to assess the impact of each scenario on system operation in mitigating the forecasting errors of wind power plants. Further, a comparative analysis was performed to illustrate the effectiveness of each case study. The analysis showed that Case Study III, where reserves are provided by coal energy systems and cold storage units, yielded the highest reduction in Positive Regulation (PR) and Negative Regulation (NR) errors, at 89.0% and 94.15%, respectively. Conversely, Case Study IV demonstrated the least reduction in errors, with 67.82% in PR and 78.41% in NR. However, it indicates that reserves can be supplied from wind energy systems and flexible loads without the support of conventional power plants
Gelir belirlenmesinin basit matematiği
No full textKonjonktür dalgalanmaları ile ilgilenen ileri düzeydeki öğrenciler, çok çeşitli tipteki "gelir çoğaltanlarını ( çarpanları)" hesaplamada profesyonel hale gelmelerini büyük ölçüde Profesör Hansen'in ve yardımcılarının katkılarına borçludur. Gerçekte konu son zamanlarda bir kara sanat haline gelmiştir. Kara, çünkü konuyla ilgili olmayanlar için bu teknik kavram, muhakkak ki muzır olmuş bile mistik görünmektedir; sanat, çünkü bununla çok yakından ilgili olanlar dahi belirli bir çoğaltan formülünün gerektirdiği tüm karmaşık terimleri hatırlamada oldukça büyük güçlüklerle karşılaşmaktadırlar. Gelir belirlenmesi ile ilgili aşırı ölçüdeki basitleştirici varsayımları düşürdüğümüzde ve iktisadi politika veya gerçek hayata ilişkin çok çeşitli soruları yanıt aramaya başladığımızda, bu karmaşıklığın gerçekten konu içinde saklı bulunduğu görülecektir. Fakat sorun sadece bu kadarla da kalmamaktadır. Hoca veya öğrenci ısından bakıldığında , konudaki' güçlüğün büyük bir bölümü çoğaltan ifadelerinin ortaya çıkmasına neden olan denge şartları üzerine konsantre olmak yerine "çoğaltanları" çıkarma işi ile uğraşmaktan kaynaklanmaktadır. Mantıki olarak geliri belirleyen ilişkiler, denge gelirinin değişme şeklini tasvir etmeden daha önce gelmektedir. Keza bunları hatırlamak ve çok dar ve katı varsayımlar yapmaksızın ele almak daha kolaydır. Ayrıca, bunlar belirli herhangi bir problem için uygun çoğaltanın kolay bir şekilde çıkarılmasına olanak sağlamaktadır. Buradaki tartışma salt açıklama niteliği taşımakta ve ileri düzeydeki literatür de tamamen konu dışı bırakılan problemleri ele almaktadır. Analiz çok basit bir matematiksel dil kullanarak (1) "tasarruf ve yatırım"ın geliri belirlediği en basit Keynesgil modeli, (2) devlet harcamalarının ve vergilerin bu tabloya nasıl girdiğini, ( 3) uluslararası ticaretin rolünü, ve ( 4) şirketlerin ve bunların tasarruflarının nasıl ele alınabileceğini göstermeye çalışmaktadır
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
ARCHITECTED MATERIALS FOR FUNCTIONAL PROPERTIES
No full textQuesta tesi analizza l'influenza della morfologia dei nanomateriali e dell'ingegneria interfacciale sulla dinamica del trasferimento di carica in sistemi eterostrutturati. Lo studio inizia con una valutazione sistematica di nanoparticelle di TiO2 e di array di nanotubi di TiO2 (TNTAs), rivelando che i TNTAs in fase anatase con un'altezza ottimizzata di 8 μm mostrano un'attività superiore nella reazione di evoluzione dell'idrogeno (HER), grazie a percorsi di trasporto di carica migliorati e a proprietà elettroniche favorevoli. Successivamente, è stato esplorato il ruolo della chimica superficiale nel controllo del trasferimento di carica interfacciale utilizzando nanocristalli di perovskite CsPbBr3 (PNCs) con lunghezze di catena dei leganti controllate. I leganti a catena corta hanno facilitato un trasferimento elettronico più efficiente verso le nanostrutture di TiO2, con gli array di nanotubi che hanno superato le pellicole di nanoparticelle nelle configurazioni allo stato solido. Le perovskiti drogati con Mn hanno ulteriormente evidenziato il comportamento di quenching dipendente dalla morfologia del TiO2, sebbene approfondimenti più dettagliati sulla dinamica dei portatori di carica richiedano tecniche spettroscopiche avanzate. Infine, i quantum dots core–shell CdTe/CdSe sono stati interfaccati con morfologie di TiO2, rivelando comportamenti contrastanti tra le fasi in soluzione e in film sottile. In soluzione, le nanoparticelle di TiO2 hanno promosso un quenching più marcato, mentre nei film sottili, i TNTAs hanno consentito un'estrazione di carica più efficiente e un trasporto direzionale, portando a una degradazione fotocatalitica efficace del colorante Rhodamine B in soluzione acquosa. Complessivamente, questo lavoro dimostra che l'ottimizzazione della morfologia, della fase e della chimica superficiale dei nanomateriali è fondamentale per migliorare i processi di trasferimento di carica e l'attività fotocatalitica. Le conoscenze acquisite offrono una strada per la progettazione di nanostrutture ibride ad alte prestazioni per la generazione fotocatalitica di idrogeno e per applicazioni optoelettroniche correlate.This thesis investigates the influence of nanomaterial morphology and interfacial engineering on charge transfer dynamics in a heterostructure system. The study begins with a systematic evaluation of TiO2 nanoparticles and nanotube arrays (TNTAs), revealing that anatase-phase TNTAs with an optimized height of 8 μm exhibit superior hydrogen evolution reaction (HER) activity, driven by enhanced charge transport pathways and suitable morphology. Subsequently, the role of surface chemistry in governing interfacial charge transfer was explored using CsPbBr3 perovskite nanocrystals (PNCs) with controlled ligand chain lengths. Short-chain ligands facilitated more effective electron transfer to TiO2 nanostructures, with nanotube arrays outperforming nanoparticle films in solid-state configurations. Mn-doped perovskites further highlighted the morphology-dependent quenching behaviour of TiO2, though deeper insights into charge carrier dynamics require advanced spectroscopic techniques. Finally, CdTe/CdSe core–shell quantum dots were interfaced with TiO2 morphologies, revealing contrasting behaviours between solution and thin-film phases. In solution, TiO2 nanoparticles promoted stronger quenching, while in thin films, TNTAs enabled more efficient charge extraction and directional transport leading to efficient photocatalytic degradation of aqueous Rhodamine B dye. Collectively, this work demonstrates that fine-tuning nanomaterial morphology, phase, and surface chemistry is critical to optimizing charge transfer processes and photocatalytic activity. The insights gained may provide a pathway for designing high-performance, hybrid nanostructures for photocatalysis and other optoelectronic applications
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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