128 research outputs found
„Sublokatorka” po latach. Z Hanną Krall rozmawiają Elżbieta Janicka i Joanna Tokarska-Bakir. Warszawa, 28 lutego i 8 marca 2013 roku
No full text"Sublokatorka" now. An interview with Hanna Krall: Warsaw, 28 February and 28 March 2013
The interview was conducted by Elżbieta Janicka and Joanna Tokarska-Bakir. Hanna Krall told the story of the issues related to the publication of Sublokatorka, which finally appeared in the Paris-based printing house Libella, owned by Zofia and Kazimierz Romanowicz, in 1985. Hanna Krall also spoke about how the book was received by the public in the unofficial underground discourse in the Communist Poland. Moreover, the author of Sublokatorka told the interviewers about the construction of stories about the Holocaust, as well as about the people who inspired the protagonists of Sublokatorka: Luba Bielicka-Blum, Rywka Urman, Krystyna Krahelska, Krzysztof Kieślowski and Jerzy Stuhr.
„Sublokatorka” po latach. Z Hanną Krall rozmawiają Elżbieta Janicka i Joanna Tokarska-Bakir. Warszawa, 28 lutego i 8 marca 2013 roku
Hanna Krall opowiada Elżbiecie Janickiej i Joannie Tokarskiej-Bakir o kłopotach z publikacją Sublokatorki, która ostatecznie ukazała się w paryskim wydawnictwie Zofii i Kazimierza Romanowiczów Libella w 1985 roku. Tematem rozmowy jest także burzliwy, niewolny od werbalnej agresji, odbiór książki w drugim, niezależnym obiegu kultury w PRL. W wywiadzie mowa ponadto o sposobach konstruowania i stawkach opowieści o Zagładzie, a także o pierwowzorach postaci z Sublokatorki takich jak: Luba Bielicka-Blum, Rywka Urman, Krystyna Krahelska, Krzysztof Kieślowski czy Jerzy Stuhr
ILUMINASI NASKAH MELAYU KARYA M. BAKIR KOLEKSI PNRI: TINJAUAN SEMIOTIKA UMBERTO ECO
No full textThe objective of this research is to point out types of illumination in Malay
manuscripts stored in PNRI, to point out the connection between manuscript
illumination and its author or scribe, and to point out the motifs on manuscript
illumination using codicology. Furthermore, this research is conducted to identify the
significance of motifs in Malay manuscripts illumination written by Muhammad
Bakir with regard to the texts and society at the time of making. This significance is
analysed using Umberto Eco�s semiotics. This research also has the objective of
pointing out the tradition of manuscript visualisation in the form of types of
illumination in Malay manuscript as society�s creativity in the past.
This research applies the theories of philology and semiotics. The theory of
philology is used to identify narrative aspects and its illumination. Meanwhile, the
theory of semiotics is used to reveal the significance of motifs in Malay manuscript
illumination written by Muhammad Bakir with regard to the texts and other
contextual things.
The result of the analysis shows that there are various styles and motifs in
Malay manuscript illumination stored in PNRI. The diversity of motifs in Malay
manuscript illumination indicates that Malay society in the past had self-effort
approach to the Creator and the universe. The research also shows that most of the
illuminated Malay manuscripts stored in PNRI are anonymous. Based on the
domination of manuscripts which have complete information about manuscript, the
manuscripts illumination written by Muhammad Bakir is used to identify the
existence of significance of motifs in the illumination.
Based on semiotical analysis, it can be inferred that the motifs in manuscript
illumination written by Muhammad Bakir originate from the developing values inside
Malay-Betawi society and Muhammad Bakir�s experience as a writer and a person
who rented manuscripts. Some of the motifs seen in manuscript illumination written
by Muhammad Bakir become the local wisdom for Betawi society until now. In other
words, there is continuity between the manuscript in one period and the life of society
afterward
The Role Of P120catenin In The Initiation And Dissemination Of Pancreatic Cancer
No full textPancreatic cancer is the third leading cause of cancer mortality, the overwhelming majority of which is due to metastasis. The mechanisms of dissemination have been intensively studied, but questions remain as to how epithelial properties are regulated between the primary tumor site and destination as well as to how organotropic patterns of metastasis are determined. We demonstrate, using multiple complementary mouse models, that liver and lung metastatic organotropism in pancreatic cancer is dependent on p120catenin (p120ctn)-mediated epithelial identity. Monoallelic p120ctn loss accelerates KrasG12D-driven PanIN/PDAC formation and metastasis to the liver as well as being sufficient for E-CADHERIN-mediated cell adhesion. In contrast, cells with biallelic p120ctn loss demonstrate marked lung organotropism, although rescue with p120ctn restores liver metastasis. In a p120ctn-independent PDAC model, mosaic E-cadherin knockout shows selective pressure for E-cadherin-positive liver metastasis and E-cadherin-negative lung metastasis. Furthermore, human PDAC and liver metastases support the premise that liver metastases exhibit predominantly epithelial characteristics. RNA-seq demonstrates differential induction of pathways associated with metastasis and epithelial-to-mesenchymal transition in p120ctn-deficient versus p120ctn-wild-type cells. Taken together, p120ctn-mediated epithelial plasticity may be added to the list of emerging concepts underlying metastatic organotropism
Women’s Wages, Postdoc Woes, and Fabled Arctic Passage Opens in Northern Canadian Waters
No full textp.
Comparative Review of Science Teachers’ and Prospective Science Teachers’ Awareness, Efficacy and Attitudes and Opinions towards STEM
No full textThis research aims to compare the awareness, efficacy and attitudes, and opinions of science teachers (STs) and prospective science teachers (PSTs) toward STEM. In this study, convergent parallel design from mixed methods was used. The study group consists of 45 STs and 177 PSTs. In the quantitative dimension of the study, “Teacher Efficacy and Attitudes Towards STEM Survey” and “STEM Awareness Scale ” were applied to the study group, which was formed by sampling criteria and maximum diversity from purposeful sampling. In the qualitative dimension of the research, semi-structured interviews were conducted with 24 STs and 25 PSTs selected through maximum diversity sampling from purposeful sampling. As a result of the research, it was observed that although the STEM awareness, efficacy, and attitudes of STs and PSTs were similar, they had positive attitudes. However, most of the participants in both groups considered themselves inadequate in STEM, expressed their opinions about the adverse effects of STEM applications as well as their positive effects, and that STEM applications were effective in career selection and country development
ANALISIS PARATEKSTUAL TERHADAP HIKAYAT WAYANG ARJUNA KARYA MUHAMMAD BAKIR
No full textThe aim of this study is to know how the paratext elements in Hikayat Wayang
Arjuna manuscript written by Muhammad Bakir take a part to show preview about the
content of the text. Paratext elements in this manuscript also take a part in relation to the
HWA manuscript rental. Paratextuality in this manuscript are important to be research
because can give preview about the text content toward manuscript renter candidates.
The object of this study is the paratext elements of Hikayat Wayang Arjuna
which code is Ml.244. Paratext elements in this manuscript, which will be talk in this
tesis, include formats, signs and notes, name of the author, illustration, date, epigraph,
please insert, cover, preface, and private epitext.
Hikayat Wayang Arjuna tells about Rajuna who despised by kings and male
deities because of his behavior and his statement that he was �lanang sejagat sejagat
tiyang lanang�. In this story, Arjuna has fantastic story, for examples are that he is alive
after his head had cut, Arjuna become four and be the trouble makers in the earth and
�kayangan�. Because of Semar and Punakawan, the trouble can be captured and
finished.
When doing this research, which refers to Genette, three adaptations are doing
related to paratext elements: Rubrication, date, and illustration. Rubricate is a paratext
element which not mention by Genette in his book. But here, in this thesis, rubricate is
include because of its function which is to sharp the text. Ilustration and date are
paratext elements which are mention by Genette but they are not being talk more by
Genette in his book.
Philology and Literary approach are used for achieved the aim of this research.
Philology approach is used for solving the problems which are related to the manuscript
and text transliteration. Literary approach, especially Gerard Genette theory of Paratext,
is used for analyze paratext elements in the Hikayat Wayang Arjuna manuscript whose
code is Ml.244.
Related to the paratext elements of manuscript, from the study which has been
done, we know that paratext elements are made after the text had been written. The
senders of paratext elements are Muhammad Bakir�as the author and as the
publisher�and Genootschap employees who also give notes and stamp on the
manuscript. The addressees of paratext�s elements are the holders of the manuscrip
Abstract PR04: p120 catenin loss drives pancreatic cancer EMT and metastasis through activation of calcium signaling
No full textAbstract
Purpose: We have generated a mouse model to delete the gene Ctnnd1, whose gene product p120 catenin (p120ctn) is necessary for E-CADHERIN stability, resulting in enhanced metastasis in the conventional KPC pancreatic tumor mouse model. An unbiased screen of tumor cells isolated from these mice identified misregulated calcium signaling as a previously unappreciated contributor to epithelial-to-mesenchymal transition (EMT) and metastasis. Thus, our overarching hypothesis is that p120ctn loss in pancreatic cancer drives EMT and metastasis through functional upregulation of the calcium signaling component PTHLH.
Background: Pancreatic ductal adenocarcinoma (PDAC) is a major health issue, with only 7% of patients surviving beyond 5 years, and increases in PDAC-associated deaths project this disease to be the second leading cause of cancer deaths by 2020. An unbiased approach to discover candidate cancer genes in PDAC identified the p120 catenin gene as one of the top 20 PDAC cancer genes, and further analysis revealed that p120ctn loss was associated with reduced PDAC patient survival. Recent work from our lab further demonstrated that conditional p120ctn/Ctnnd1 deletion in the esophagus was sufficient to drive invasive squamous cell carcinoma, establishing p120ctn/Ctnnd1 as a bona fide tumor-suppressor gene. Results presented herein determined the in vivo role of p120ctn loss in PDAC tumorigenesis and metastasis through genetic mouse models and unbiased RNA-seq analysis.
Methods: A PDAC mouse model was established to study the role of p120ctn in pancreatic carcinogenesis and metastasis. Specifically, LSL-KrasG12D/+; p53fl/+; Pdx1cre; Rosa26LSL-YFP; Ctnnd1fl/wt mice (herein KPCY-p120CKO) were generated to determine the effect of conditional p120ctn loss on pancreatic cancer. Furthermore, RNA-seq was performed on p120-intact or p120-null pancreatic tumor cells isolated from these mice to identify novel mechanisms of pancreatic tumorigenesis and metastasis.
Results: We demonstrate p120ctn loss as a catastrophic event for tumor epithelial cell identity in vivo, leading to enhanced EMT and metastasis. Specifically, we show that KPCY-p120CKO mice have an enhanced metastatic phenotype relative to KPCY controls. Our data therefore suggest that p120ctn is a critical factor in metastatic cell dissemination, and that p120ctn loss results in tumor cells being “locked” in a mesenchymal phenotype by failing to stabilize E-cadherin at the metastatic site. To determine the mechanism of enhanced EMT and metastasis, RNA-seq analysis of p120ctn-null tumor cells was performed, which surprisingly revealed aberrant activation of calcium signaling. Unexpectedly, two of the top five most upregulated genes in p120ctn-null cells were the secreted factor Pthlh and the kinase Camk2b, both of which are key signaling molecules involved in calcium signaling. We demonstrate that PTHLH binding to its cognate receptor leads to cytosolic calcium ion (Ca2+) release, resulting in phosphorylation and activation of CaMKII. We further establish that genetic deletion or pharmacologic inhibition of Pthlh results in proliferation and migration defects. Moreover, orthotopic implantation of KPC-PthlhNULL tumor cell lines reduced tumor growth and metastasis in vivo. Finally, we show that PDAC patients with high expression of Pthlh have significantly decreased survival, suggesting that calcium signaling may be a potent oncogenic pathway in pancreatic cancer and that blocking this pathway may be of therapeutic benefit.
Conclusions: This work has demonstrated the importance of the previously unappreciated role of calcium signaling in pancreatic cancer progression and metastasis, and future studies will look to determine the efficacy of calcium-modulating therapeutics in preclinical models of pancreatic cancer.
This abstract is also being presented as Poster A42.
Citation Format: Jason R. Pitarresi, Maximilian Reichert, Basil Bakir, Leticia Moreira, Lauren Simon, Anil K. Rustgi. p120 catenin loss drives pancreatic cancer EMT and metastasis through activation of calcium signaling [abstract]. In: Proceedings of the AACR Special Conference: Advances in Modeling Cancer in Mice: Technology, Biology, and Beyond; 2017 Sep 24-27; Orlando, Florida. Philadelphia (PA): AACR; Cancer Res 2018;78(10 Suppl):Abstract nr PR04.</jats:p
ETS-Transcription Factor ETV1 Regulates Stromal Expansion and Metastasis in Pancreatic Cancer
No full textBACKGROUND & AIMS: The ETS-transcription factor ETV1 is involved in epithelial-mesenchymal transition during pancreatic development and is induced in mouse pancreatic intraepithelial neoplasia (PanIN) and pancreatic ductal adenocarcinoma (PDAC). We investigated the function of ETV1 in stromal expansion of PDAC and metastasis, as well as its effects on a novel downstream target Sparc, which encodes a matricellular protein found in PDAC stroma that has been associated with invasiveness, metastasis and poor patient outcomes. METHODS: Pancreatic ductal cells were isolated from Pdx1Cre; Kras(G12D/+) mice (PanIN), Pdx1Cre; Kras(G12D/+); p53(fl/+) and Pdx1Cre; Kras(G12D/+); p53(fl/+); Rosa26(YFP) mice (PDAC), and Pdx1Cre; Kras(G12D/+); p53(fl/+); Sparc(-/-) mice. Cells were grown in 3-dimensional organoid culture to analyze morphology, proliferation, and invasion. Human PanIN and PDAC tissues were evaluated for ETV1 expression. Orthotopic pancreatic transplants of ETV1-overexpressing PDAC and respective control cells were performed. RESULTS: ETV1 expression was significantly increased in human PanINs and, even more so, in primary and metastatic PDAC. Analyses of mouse orthotopic xenografts revealed that ETV1 induced significantly larger primary tumors than controls, with significantly increased stromal expansion, ascites and metastases. In 3-dimensional organoids, ETV1 disrupted cyst architecture, induced EMT, and increased invasive capacity. Furthermore, we identified Sparc as a novel functional gene target of Etv1 by luciferase assays, and SPARC and ETV1 proteins co-localized in vivo. Disruption of Sparc abrogates the phenotype of stromal expansion and metastasis found with ETV1 overexpression in vivo. We identified hyaluronan synthase 2 (Has2) as another novel downstream factor of Etv1; that may mediate ETV1's significant expansion of hyaluronic acid in PDAC stroma. Conversely, disruption of Etv1 in PDAC mice (Pdx1Cre; Kras(G12D/+); p53(fl/+); Rosa26(YFP); Cre; Etv1(fl/fl)) reduced levels of SPARC and hyaluronic acid in the stroma. CONCLUSIONS: ETV1 is critical in the desmoplastic stromal expansion and metastatic progression of pancreatic cancer in mice, mediated functionally in part through Sparc and Has2
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