25 research outputs found
Chapter 9: Human Behaviour Analysis and Modelling: A Mixed Method Approach
No full textToday the ergonomic assessment of objects and products is often treated with separation of the different components of the interaction: physical, sensory, subjective. This research aims to develop an integrated method and a protocol for the qualitative and quantitative study of motor functions for ergonomics. that is addressed to evaluation of strategies and efforts carried out by users in relation with products. In our case study these products are the home appliances and in particular the dishwater. Basic methodological approaches refer to biomechanics and product usability assessment techniques. The first one is based on the at measurement of angular excursions of the joints associated with the implementation of the human motion detectable in dedicated laboratory; the second methods rely on experiments with users and direct observations and questionnaires / interviews to quantify ease of use and user satisfaction by means of special scales of assessments carried out in usability laboratories. The integration of methods for a global and more comprehensive ergonomic assessment is the rationale of the research, that can then be used in proactive way in the early stages of development
Human behaviour analysis and modelling: a mixed method approach
No full textToday the ergonomic assessment of objects and products is often treated with separation of the different components of the interaction: physical, sensory, subjective. This research aims to develop an integrated method and a protocol for the qualitative and quantitative study of motor functions for ergonomics. that is addressed to evaluation of strategies and efforts carried out by users in relation with products. In our case study these products are the home appliances and in particular the dishwater. Basic methodological approaches refer to biomechanics and product usability assessment techniques. The first one is based on the at measurement of angular excursions of the joints associated with the implementation of the human motion detectable in dedicated laboratory; the second methods rely on experiments with users and direct observations and questionnaires / interviews to quantify ease of use and user satisfaction by means of special scales of assessments carried out in usability laboratories. The integration of methods for a global and more comprehensive ergonomic assessment is the rationale of the research, that can then be used in proactive way in the early stages of development
Social housing e retail a Milano
No full textLAUREA MAGISTRALEIl titolo di questa tesi progettuale, Social housing e retail a Milano, descrive il tema funzionale e l’ambito geografico che ho approfondito per questo esame finale. Nell’ambito di Area di Trasformazione Urbana (ATU) Piazza d’Armi, situata nella zona ovest di Milano, si trovano la Caserma Santa Barbara di piazzale Perrucchetti e l’area addestrativi chiamata Piazza d’Armi in via delle Forze Armate, la cui dismissione è già decisa da tempo da parte del Ministero della Difesa in accordo con il Comune di Milano. Il progetto propone dapprima un Masterplan per il comparto che attualmente ospita le residenze dei militari di stanza alla Caserma e l’Ospedale Militare. Densità abitativa e qualità della vita degli abitanti sono ricercate mediante un alternarsi di tipologie e funzioni diverse degli edifici costruiti e degli spazi pubblici e privati. Il progetto quindi approfondisce le tematiche del social housing e della residenza in generale nel caso di un edificio a torre e di uno in linea, specialmente sotto gli aspetti della flessibilità abitativa e dell’abitare in altezza. A riguardo si è provveduto a rinvenire un ventaglio di contributi teorici e progettuali. L’edificio a torre rappresenta l’oggetto principale di interesse, definito dai punti di vista compositivo, strutturale, impiantistico e tecnologico: diverse discipline che si intersecano per definire la qualità di una torre residenziale europea che instaura una giusta relazione con gli edifici medi che la circondano e diventa un valido punto di riferimento a scala urbana
Superfície polinomial de resposta num ensaio de adubação com níveis não equidistantes
No full textA regressão polinomial é comumente aplicada em ensaios de adubação, mas quase sempre com níveis equidistantes de fertilizantes. Neste trabalho estudamos um ensaio fatorial de 3 x 3 x 3 com N, P e K, em níveis não equidistantes, que. eram os seguintes: N: zero, 30, 50 kg/ha, P2O5: zero, 45, 60 kg/ha, K2O. Parte da teoria, mostrada para o caso de níveis igualmente espaçados, foi adaptada para sua aplicação no caso estudado. A equação de regressão obtida foi: (Descrito na Tese). Os intervalos confiança obtidos de para os parâmetros foram relativamente grandes, o que concorda com grande parte dos trabalhos constantes da bibliografia. As estimativas das produções obtidas através da equação de regressão foram relativamente boas, com intervalos de confiança bem pequenos. A análise de variância mostrou efeito altamente significativo para a regressão. O coeficiente de determinação foi de 0,949, ou seja, 94,9% da variação foi explicada pela regressão. Os testes dos parâmetros foram altamente significativos em todos os casos, coro exceção para os parâmetros a22 e a33. Isto mostra, que para P e K a produção cresce de modo aproximadamente linear dentro dos limites do ensaio. A receita liquida, dada pela equação: Z= wŷ - t1 x1 - t2 x2 - t3 x1 - m foi também estudada, com os valores seguintes: w (preço do milho): 0,42 bolivares/kg, t1 (preço do N): 13,6 bolivares/kg, t2 (preço do P2O5): 7,0 bolivares/kg, t<sub1 (preço do K2O): 4,4 bolivares/kg. Esta função, para o ensaio estudado, não possui máximo, mas sim um ponto de sela. Entretanto, se considerarmos somente os valores contidos dentro dos intervalos usados no experimento, há um máximo absoluto na função da receita liquida, para x1= 4,94, x2= 6,00 x3= 5,00. Assim podemos recomendar no presente caso, as seguintes doses de nutrientes: 49,4 kg/ha de N, 60,0 kg/ha de P2O5, 50,0 kg/ha de K2O.Polynomial regression is commonly appllied in the and.lysis of fertilizer experiments, but almost always with equally spaced levels. The author studies a 3x3x3 N, P, K factorial trial with unequally spaced levels, which were the followingg: N: zero, 30, 50 kg/ha, P2O5: zero, 45, 60 kg/ha, K2O. First of all the theory, shown for the case of equally spaced levels, was modified in order to be applied to the case under study. The regres.sion equation obtained was: (See Thesis). The confidence intervals for the parameters were relatively large, which is in good agreement with many of the references. The harvest estimates obtained by the use of the regression equation were good, with rather short confidence intervals. The analysis of variance gave a highly significant F for regression. The coefficient of determination was 0.949, that is, 94.9% of the variation were explained by the regression equation. Tests for the parameters were highly significant in all cases, except for a22 and a33. This shows, of course, that for P and K the response is approxirnately linear within the limits of fertilization ín the experiment. The net income, give by equacion: Z= wŷ - t1 x1 - t2 x2 - t3 x1 - m, was studied also, with the following values: w (price of maize): 0,42 bolivares/kg, t1 (price of N): 13,6 bolivares/kg, t2 (price of P2O5): 7,0 bolivares/kg, t<sub1 (price of K2O): 4,4 bolivares/kg. The net income function has no ma:ximum, but a saddle point. However, if we consider only the values within the intervals used in the expe- riment, there is an absolute maximum income for x1= 4,94, x2= 6,00 x3= 5,00. So, we should recommend, in the present case, the following levels of nutrients: 49,4 kg/ha de N, 60,0 kg/ha de P2O5, 50,0 kg/ha de K2O
Fluopsin C for treating multidrug-resistant infections: In vitro activity aganist clinically important strains and in vivo efficacy against carbapenemase-producing Klebsiella pneumoniae
No full textThe increasing emergence of multidrug-resistant (MDR) organisms in hospital infectionsis causing a global public health crisis. The development of drugs with effective antibioticaction against such agents is of the highest priority. In the present study, the actionof Fluopsin C against MDR clinical isolates was evaluated underin vitroandin vivoconditions. Fluopsin C was produced in cell suspension culture ofPseudomonasaeruginosaLV strain, purified by liquid adsorption chromatography and identified bymass spectrometric analysis. Bioactivity, bacterial resistance development risk againstclinically important pathogenic strains and toxicity in mammalian cell were initiallydetermined byin vitromodels.In vivotoxicity was evaluated inTenebrio molitorlarvae and mice. The therapeutic efficacy of intravenous Fluopsin C administration wasevaluated in a murine model ofKlebsiella pneumoniae(KPC) acute sepsis, using sixdifferent treatments. Thein vitroresults indicated MIC and MBC below 2μg/mL and lowbacterial resistance development frequency. Electron microscopy showed that FluopsinC may have altered the exopolysaccharide matrix and caused disruption of the cell wallof MDR bacteria. Best therapeutic results were achieved in mice treated with a singledose of 2 mg/kg and in mice treated with two doses of 1 mg/kg, 8 h apart. Furthermore,acute and chronic histopathological studies demonstrated absent nephrotoxicity andmoderate hepatotoxicity. The results demonstrated the efficacy of Fluopsin C againstMDR organisms inin vitroandin vivomodels, and hence it can be a novel therapeuticagent for the control of severe MDR infectionspublishedVersion© 2019 Navarro, Simionato, Pérez, Barazetti, Emiliano, Niekawa, Andreata, Modolon, Dealis, Araújo, Carlos, Scarpelim, da Silva, Chryssafidis, Bruheim and Andrade. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
Corrigendum to ‘Gene mutational profile of BRCAness and clinical implication in predicting response to platinum-based chemotherapy in patients with intrahepatic cholangiocarcinoma’ [Euro J Cancer 171 (2022) 232–241, (S0959804922002829), (10.1016/j.ejca.2022.05.004)]
No full textThe authors regret that Carles Fabregat-Franco's name was incorrectly given in the original article as Carles Fabregat. This has now been corrected in the online article. The correct author details are: Carles Fabregat-Franco bb Gastrointestinal Cancer Unit, Vall d'Hebron University Hospital & Vall d'Hebron Institute of Oncology (VHIO), Barcelona, Spain The authors would like to apologize for any inconvenience caused
In Vitro Erythropoiesis at Different pO<sub>2</sub> Induces Adaptations That Are Independent of Prior Systemic Exposure to Hypoxia
No full textHypoxia is associated with increased erythropoietin (EPO) release to drive erythropoiesis. At high altitude, EPO levels first increase and then decrease, although erythropoiesis remains elevated at a stable level. The roles of hypoxia and related EPO adjustments are not fully understood, which has contributed to the formulation of the theory of neocytolysis. We aimed to evaluate the role of oxygen exclusively on erythropoiesis, comparing in vitro erythroid differentiation performed at atmospheric oxygen, a lower oxygen concentration (three percent oxygen) and with cultures of erythroid precursors isolated from peripheral blood after a 19-day sojourn at high altitude (3450 m). Results highlight an accelerated erythroid maturation at low oxygen and more concave morphology of reticulocytes. No differences in deformability were observed in the formed reticulocytes in the tested conditions. Moreover, hematopoietic stem and progenitor cells isolated from blood affected by hypoxia at high altitude did not result in different erythroid development, suggesting no retention of a high-altitude signature but rather an immediate adaptation to oxygen concentration. This adaptation was observed during in vitro erythropoiesis at three percent oxygen by a significantly increased glycolytic metabolic profile. These hypoxia-induced effects on in vitro erythropoiesis fail to provide an intrinsic explanation of the concept of neocytolysis.BT/Bioprocess Engineerin
