49 research outputs found
Estudo De Viabilidade Da Utilização Da Tecnologia Power Line Communication - PLC Em Redes Locais Em Comparativo Com Cabo De Par Trançado
No full textTCC (graduação) - Universidade Federal de Santa Catarina. Campus Araranguá. Tecnologias da Informação e comunicaçãoO presente trabalho tem por objetivo analisar a viabilidade da utilização da tecnologia PLC em redes locais em comparativo com cabo metálico de par trançado UTP. O estudo aborda a tecnologia PLC para acesso indoor. No transcorrer da pesquisa faz-se uma breve introdução sobre o PLC contando um pouco de sua história, princípios de funcionamento, principais aplicações e topologias. Também é feita uma breve introdução sobre a tecnologia de transmissão de dados por meio de cabos metálicos de par trançado UTP, contando um pouco de sua história, princípios de funcionamento, categorias e tipos de cabos existentes. O foco da pesquisa destina-se a analisar a viabilidade de utilização da tecnologia PLC em redes locais onde a passagem de cabos metálicos de par trançado não é possível ou viável devido a barreiras físicas existentes e entre outros, como por exemplos dutos para passagem dos cabos lotados ou distância entre as partes a serem interligadas. Para tal conclusão foi realizado um estudo de caso em bancada de laboratório analisando e comparando as duas tecnologias mediante os testes de HTTP, HTTP de página completa, ping para gateway, RDP e velocidade. De acordo com a pesquisa a tecnologia PLC mostra-se eficaz, e se conclui que é viável a utilização de PLC nas redes locais.This study aims to analyze the feasibility of the use of PLC technology in local networks in comparison with UTP twisted pair of wire-rope. The study addresses the PLC technology for indoor access. During the study makes a brief introduction to the PLC telling a little of its history, operating principles, main applications and topologies. It is also made a brief introduction about data transmission technology through twisted-pair UTP copper cables, telling a little of its history, operating principles, categories and types of cables. The focus of the research is intended to analyze the feasibility of use of PLC technology in local networks where the passage of twisted-pair copper cables is not possible or feasible due to physical barriers and among others, for examples ducts for passage of cables blended or distance between the parts to be interconnected. For this conclusion was carried out a case study in lab bench analyzing and comparing the two technologies through the HTTP tests, full page HTTP, ping to gateway, RDP and speed. According to the survey the PLC technology is effective, and it is concluded that the use of PLC in local networks is feasible
Abstract A15: Establishing an ex-vivo, tumor spheroid culture system to assess molecular-targeted therapies for personalized treatment of non-small cell lung cancer
No full textAbstract
Molecularly-targeted therapeutics are being developed for personalized cancer treatment based on the ideas that treatments should be determined by specific characteristics of an individual patient's tumor and that treatments should target tumor cells without damaging normal cells. Accumulating evidence indicates tumor heterogeneity can lead to acquired resistance to molecularly-targeted therapies. Currently, preclinical investigations rely heavily on the use of tumor cell lines and mouse xenograft models. The high failure rate in oncology drug development suggests that such test systems do not adequately model tumor heterogeneity. Therefore, more predictive preclinical models are needed to ensure that only the most promising molecularly-targeted, combination therapies are assessed in clinical studies. Here we established a novel 3D lung adenocarcinoma tissue-originating spheroid model, which may be used to investigate the efficacy of molecularly-targeted cancer therapeutics. Fresh lung adenocarcinomas were minced and either digested with Liberase DH enzyme (5.6 units) or homogenized to generate cancer tissue-originating spheroids. Spheroids were plated in Matrigel and cultured in stem cell media, with 0.01, 0.1, 1 or 10 μM erlotinib or the DMSO vehicle. Erlotinib is an epidermal growth receptor inhibitor commonly used to treat non-small cell lung cancer (NSCLC). Using an ImageXpress Micro Widefield High Content Screening System (Molecular devices), z-stack bright field images were collected 1, 7, and 14 days after plating. Composite z-stack images were analyzed using LASX (Leica Microsystems) software. Total spheroid area (in pixels) was measured for replicate cultures exposed to different erlotinib concentrations at each time point. The total area of spheroids for 7 and 14 days were normalized to day 1 measurements and cell death was evaluated as the decrease in total area of the spheroids in culture. Our results indicate that direct homogenization of lung adenocarcinomas generated at least ten times greater yield of spheroids than was achieved using digestion with the Liberase DH. The yield and appearance of spheroids varied significantly. Spheroids prepared from some lung adenocarcinomas were more spherical and aggregated than those prepared from other tumors. There was time-dependent decrease in total area of spheroids following 7 and 14 days in culture. Also spheroids prepared from different lung adenocarcinomas responded differently to different concentrations of erlotinib. High-sensitivity quantification of specific mutations (EGFR, KRAS PIK3CA and BRAF) will be conducted before and after erlotinib treatments to determine whether this model can be used to assess the potential outgrowth of drug-resistant tumor subpopulations.
Citation Format: Malathi Banda, Karen L. McKim, Barbara Parsons. Establishing an ex-vivo, tumor spheroid culture system to assess molecular-targeted therapies for personalized treatment of non-small cell lung cancer. [abstract]. In: Proceedings of the AACR Special Conference: Patient-Derived Cancer Models: Present and Future Applications from Basic Science to the Clinic; Feb 11-14, 2016; New Orleans, LA. Philadelphia (PA): AACR; Clin Cancer Res 2016;22(16_Suppl):Abstract nr A15.</jats:p
Finding Gampöng : space, place, and resilience in post-tsunami Aceh
No full textAlso published in “Tsunami in a time of war: aid, activism & reconstruction in Sri Lanka and Aceh,” de Alwis, Malathi and Hedman, Eva-Lotta E., eds. (2009)Two communities in peri-urban Banda Aceh were observed during post-tsunami displacement, and upon return to their original villages. Social cohesiveness prior to disaster, leadership during the emergency period and afterwards, as well as interaction with outside intervention over programs and projects, were among the main factors shaping a new community in a “new” settlement. This paper shows how Acehnese use their social networks to cope with calamities in relation to the concept of gampöng: a spatial and cultural concept of community. Sense of community and interconnectedness among its members is the soul that makes a space a “place” to dwell in
Evaluating stucturally different pectic oligosaccharides in inhibiting adhesion of e. coli 0157:H7 to human gut epithelial cells in vitro
No full textBacterial adhesion to glycosylated cellular surfaces is a major concern in human health and disease. Inhibition of bacterial adhesion by suitable carbohydrates may lead to an anti-adhesion therapy as a novel prophylactic approach against bacterial infections and a potential alternative to the use of antibiotics. Selections of six pectic oligosaccharides derived from citrus peel albedo, which were different in terms of their monosaccharide composition and physical properties, were evaluated for their ability to interfere with the adhesion of Escherichia coli O157:H7 to HT29 cells in vitro. Attachment was determined in the human HT29 cell line by viable count of adherent bacteria. Most of the pectic oligosaccharides in buffer at pH 7.2 were anti-adhesive at a dose of 0.001 - 0.05 mg/ml, reducing adhesion of E.coli by 50 - 90% and concentrations of 0.5 - 5 mg/ml resulted in less than 50% reduction of adhesion to no effect. Based on the results, lower concentrations were more effective in reducing adhesion when compared to the higher concentrations. The pectic oligosaccharides with a homogalacturonan structure, low molecular weight and lower degree of esterification were the most effective in reducing the adhesion when compared to the oligosaccharides with an arabinose rich rhamnogalacturonan structure with higher molecular weight and higher degree of esterification. These results show that the pectic oligosaccharides with different monosaccharide composition and physical properties can display a wide range of anti-adhesive activity.M.S.Includes bibliographical referencesby Malathi Srilakshmi Vakkalank
A Cross-Layer Multicast-Push Unicast-Pull (MPUP) Architecture for Reliable File-Stream Distribution
No full textThe growing deployment of OpenFlow/SDN networks makes it increasingly possible to leverage network multi-cast services. This work proposes a novel cross-layer Multicast- Push Unicast Pull (MPUP) architecture that includes functionality in the application, transport and link layers to offer users a reliable file-stream distribution service to multiple subscribers. A prototype implementation of the MPUP architecture was realized in a new version of Local Data Manager (LDM), LDM7, a software program that has been in use since 1994 for real-time meteorology data distribution. LDM6, the currently deployed version, uses application-layer multicast. Experiment were run on the GENI infrastructure to compare LDM7 and LDM6. The two main findings are (i) LDM7 can be run at a higher sending rate than LDM6 allowing for improved performance (lower file-delivery latency), and (ii) to achieve the same performance, LDM7 uses significantly lower bandwidth and compute capacity. A three-fold improvement in performance improvement was possible with LDM7, and a bandwidth reduction from 350 Mbps to 21.4 Mbps was observed with 24 receivers.Peer reviewe
Computational model based approach to analysis ventricular arrhythmias: Effects of dysfunction calcium channels
No full text
Studies on effect of Various Operating Parameters and Foaming Agents on the Foam-Mat Drying of Fruit Pulps
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Isolation of Glycosaminoglycans(GAG) from Red Blood Cells of Rat
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
