175 research outputs found
Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates
No full text© 2003 Ivell et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.METHODS: Three peptide sequences were identified from the proposed open reading frame of the cloned LGR7 receptor gene, representing both extracellular and intracellular domains. Two to three rabbits were immunized for each epitope, and the resulting sera subjected to a systematic validation using cultured cells transiently transfected with a receptor-expressing gene construct, or appropriate control constructs. RESULTS: Human and monkey (marmoset, macaque) endometrium showed consistent and specific immunostaining in the stromal cells close to glands. Staining appeared to be more intense in the luteal phase of the cycle. Weak immunostaining was also evident in the endometrial epithelial cells of the marmoset. A myoma in one patient exhibited strong immunostaining in the circumscribing connective tissue. Uterine expression was supported by RT-PCR results from cultured primary endometrial and myometrial cells. Human breast tissue (healthy and tumors) consistently indicated specific immunostaining in the interstitial connective (stromal) tissue within the glands, but not in epithelial or myoepithelial cells, except in some tumors, where a few epithelial and tumor cells also showed weak epitope expression. CONCLUSIONS: Using validated monotypic antibodies recognizing different epitopes of the LGR7 receptor, and from different immunized animals, and in different primate species, a consistent pattern of LGR7 expression was observed in the stromal (connective tissue) cells of the endometrium and breast, consistent also with the known physiology of the relaxin hormone.Richard Ivell, Marga Balvers, Yvonne Pohnke, Ralph Telgmann, Olaf Bartsch, Karin Milde-Langosch, Ana-maria Bamberger, and Almuth Einspanie
SPEER - A new family of testis-specific genes from the mouse
No full text© 2003 by the Society for the Study of ReproductionAndrej-Nikolai Spiess, Norbert Walther, Nadine Muller, Marga Balvers, Christoph Hansis, and Richard Ivel
Testis-specific expression of rat mitochondrial glycerol-3-phosphate dehydrogenase in haploid male germ cells
No full textJoachim M. Weitzel, Natalia B. Shiryaeva, Ralf Middendorff, Marga Balvers, Christiane Radtke, Richard Ivell, and Hans J. Seit
Constitutive regulation of the Insl3 gene in rat Leydig cells
No full textCopyright © 2005 Elsevier Ireland Ltd All rights reserved.Insulin-like factor 3 (Insl3) is a major new product of the Leydig cells in all mammalian species so far examined. The rat Insl3 gene is encoded by two exons in close juxtaposition to the Jak3 gene. Using RT-PCR analysis we now show that in the rat testis it is expressed as both major and minor splice variants, the former encoding the normal protein, the latter a truncated peptide comprising a C-terminally extended B-domain. Both transcripts are produced in constant relative amounts uniquely in the Leydig cells of the postnatal testis and in no other testicular cell type. Rat Insl3 protein is also expressed only in Leydig cells after postnatal day 30. Although specific mRNA is present at earlier times, corresponding protein is not detected. Semi-quantitative RT-PCR analysis of Insl3 transcripts in the mouse MA-10 tumour Leydig cell-line under a wide range of stimulation regimes shows that in an acute context, the Insl3 gene is expressed absolutely constitutively. This is confirmed by transfection and electrophoretic mobility shift (EMSA) analysis of the rat Insl3 gene promoter, wherein the importance of three putative SF-1 responsive elements is underscored, although these appear to differ in their relative importance from their counterparts in the mouse Insl3 gene.Helen Sadeghian, Ravinder Anand-Ivell, Marga Balvers, Vandana Relan and Richard Ivellhttp://www.elsevier.com/wps/find/journaldescription.cws_home/506028/description#descriptio
María Elena Marqués, Miguel Torruco, Marga López y otros actores durante la entrega de premio Ariel.
No full textThe Bhakti Marga Movement: The Context of Western Hinduism
No full textDarbs koncentrējas uz Bhakti Marga kustību, kas aktīvi gūst popularitāti mūsdienu Rietumu sabiedrībā. Autors analizē šīs kustības aktualitātes cēloņus un meklē tos līdzībā ar Rietumu hinduisma iezīmēm.The work is dedicated to the Bhakti Marga movement, which is gaining popularity in contemporary Western society. The author analyzes the reasons for the relevance of this movement, finding them in the similarities with the characteristics of Western Hinduism
Implementasi Kebijakan Pembangunan Jalan pada Dinas Bina Marga Kabupaten Kuningan
No full textABSTRACTÂ The author poured this essay writing with the title "Implementation of Road Construction Policy in Kuningan Bina Marga Office. Â The problems that arise whether the implementation of road development policy in the Department of Highways District Kuningan not optimal. The objectives of this research are: (1) how Implementation of Road Construction Policy in Bina Marga Office of Kuningan Regency, (2) what factors influence the policy of Road Construction in Bina Marga Office of Kuningan Regency, (3) any obstacles faced in Implementation of Policy Implementation of Road Development at Bina Marga Office of Kuningan Regency. This study used qualitative research methods. Qualitative research method is the method used in natural object condition. The theory used in this research is George Edward III theory, which are: (1) communication, (2) sources, (3) disposition or attitude, (4) bureaucracy structure. The result shows that the Implementation of Road Construction Policy in Bina Marga Office of Kuningan Regency has not been optimally caused by many problems due to many obstacles such as delay of road construction fund delayed and can mempperlambat the process of development of the road, the attitude of the executing officers less responsible and less knowledge of the executing officers on the field so that road construction is not optimal yet
Implementasi Kebijakan Pembangunan Jalan pada Dinas Bina Marga Kabupaten Kuningan
No full textABSTRACT The author poured this essay writing with the title "Implementation of Road Construction Policy in Kuningan Bina Marga Office. The problems that arise whether the implementation of road development policy in the Department of Highways District Kuningan not optimal. The objectives of this research are: (1) how Implementation of Road Construction Policy in Bina Marga Office of Kuningan Regency, (2) what factors influence the policy of Road Construction in Bina Marga Office of Kuningan Regency, (3) any obstacles faced in Implementation of Policy Implementation of Road Development at Bina Marga Office of Kuningan Regency. This study used qualitative research methods. Qualitative research method is the method used in natural object condition. The theory used in this research is George Edward III theory, which are: (1) communication, (2) sources, (3) disposition or attitude, (4) bureaucracy structure. The result shows that the Implementation of Road Construction Policy in Bina Marga Office of Kuningan Regency has not been optimally caused by many problems due to many obstacles such as delay of road construction fund delayed and can mempperlambat the process of development of the road, the attitude of the executing officers less responsible and less knowledge of the executing officers on the field so that road construction is not optimal yet
The Description of Mangain Marga In Batak Toba Culture
No full textThe purpose of this paper is to know and understand: what the meaning of
Mangain Marga is and what the process in Mangain Marga is. The data from this
paper is a form of the tradition of the Batak society. The method used in this paper
is descriptive qualitative research, by collecting books, information from the
internet and the Toba Batak society: Traditions are values, ideas, beliefs, habits
that are passed down from one generation to the next, such as customs, arts and
others Mangain Marga is a tradition of giving a clan to someone who is not
Batak. This can happen because it is motivated by inter-ethnic marnages between
Batak and Non-Batak tribes so that the non-Batak couple will get recognition in
the family and customs as well as a position in Dalihan Na Tolu, if a clan is not
given to the non-Batak couple then he will not be recognized in customs (even)
though he is accepted in the family) and also will not get a position rights and
obligations in adat After explaining the description of the Mangain Marga
tradition in the Toba Batak culture, the author finds that a Toba Batak who wants
to marry and form a household with a non-Batak Toba partner must carry out the
Mangain Marga tradition to get a clan.53 HalamanKertas Karya Diplom
Differentiation-dependent expression of 17beta-hydroxysteroid dehydrogenase, type 10, in the rodent testis: effect of aging in Leydig cells
No full textExpression of the new 17β-hydroxysteroid dehydrogenase (HSD), type 10 (17β-HSD-10), formerly known as endoplasmic reticulum-associated amyloid-binding protein, has been investigated in the testes of various mammals under normal and perturbed conditions. Results show that 17β-HSD-10 is a major product of both fetal and adult-type Leydig cells. In the former, protein persists until late in postnatal development; and in the short-day hamster model, it does not disappear when Leydig cells involute. During puberty in the rat, immunohistochemical staining for 17β-HSD-10 in adult-type Leydig cells first becomes evident on d 20, increasing to maximal staining intensity by d 35. In the rat, but not in the mouse or any other species examined, there is also staining in late spermatids. Examination of testes from rats subjected to perinatal treatment with either a GnRH antagonist or low and high doses of diethylstilbestrol revealed that expression of 17β-HSD-10 follows closely Leydig cell differentiation status, correlating with 3β-HSD expression in a previous study. In aging (23 months) rat testes, Leydig cell (but not germ cell) immunostaining for 17β-HSD-10 is markedly reduced. 17β-HSD-10 seems to preferentially convert 3α-androstanediol into dihydrotestosterone, and estradiol to estrone. Thus, perinatal expression of this enzyme in fetal Leydig cells may contribute to protecting these cells from estrogens and encourage androgen formation.Richard Ivell, Marga Balvers, Ravinder J. K. Anand, Hans-Joachim Paust, Chris McKinnell, and Richard Sharp
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