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    Single-cell RNA sequencing for one Grave's disease and one control female

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    Single-cell RNA sequencing for one Grave's disease and one control femal

    Baek, In Gi

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    학위논문(석사)--아주대학교 일반대학원 :기계공학과,2008. 2최근 마이크로 반응기(micro reactor), 마이크로 냉각기(micro cooler), 마이크로 열교환기, 열모세관 펌프, 중합효소 연쇄반응기(polymerase chain reaction chip) 등 다양한 마이크로 디바이스에서 점점 기술의 발전으로 나노 스케일까지 접근하고 있다. 디바이스에서의 정확한 온도측정은 디바이스의 개발과 성능을 위하여 매우 중요한 인자가 된다. 온도장 측정 방법으로 과거에는 열전대(Thermocouple)를 이용한 접촉식 온도측정 방법이 주를 이루었으나, 최근에는 연구 대상의 관심영역이 나노스케일 까지 접근함에 따라 열전대를 이용한 온도측정은 불가능하게 되었다. 이에 따라 형광염료를 이용한 LIF(Laser Induced Fluorescence)기술이 온도를 측정하는 연구에 활용되고 있다. 선행연구에서 형광염료를 다양한 용매에 녹여 열유동장에 대한 온도장 측정에 사용하고 있어, 용매에 녹인 형광염료분자들은 항상 유동의 영향을 받기 때문에 고정된 지점의 온도를 측정하는데 한계가 있다. 따라서 본 연구에서는 미소디바이스의 표면온도를 측정하고자 나노 포러스 구조를 가진 박막의 기공 안에 형광염료를 주입하여 시편을 제작하였고 실험장치를 통해 온도장을 형성하여 나타나는 영역별 형광강도차이를 알아보고자 하였다. 또한 LIF기법을 응용하여 형광 나노 포러스 박막의 온도장의 측정에 적용하고자 한다. 또한 두 카메라를 통해 측정된 데이터를 바탕으로 농도, 광원의 불균일성을 배제한 형광세기가 온도만의 함수로 나타내어 정확한 온도장을 분석하고자 하였다.1. 연구배경 및 내용 = 1 2. 이론 = 3 2.1 형광(Fluorescence)의 원리 = 3 2.2 형광강도 = 3 2.3 2색 LIF기법 = 4 2.4 나노 포러스 박막 = 5 2.5 나노 포러스 박막 합성 = 6 3. 실험 장치 및 실험 방법 = 8 3.1 형광 나노 포러스 박막 = 8 3.2 실험 장치 = 9 3.2.1 형광 강도 측정 장치 = 9 3.2.2 온도 형성 장치 = 10 3.2.3 온도장 형성 장치 = 10 3.3 실험 방법 = 10 3.3.1 이미지 보정 작업 = 11 3.3.2 온도변화에 따른 형광성능 변화 측정 = 11 3.3.3 온도장 형성 및 영역별 형광강도 측정 = 12 4. 결과 및 고찰 = 13 4.1 두 광원의 분포 = 13 4.2 측정된 데이터의 수에 따른 불확실성 분석 = 13 4.3 두 방출파장대의 온도변화에 따른 형광성능곡선 = 13 4.4 측정영역별 형광세기 분석 및 보정 = 14 4.5 온도장 형성 및 형광 강도 분포 측정 = 14 5. 결론 = 16 참고문헌 = 17 ABSTRACT = 46MasterIn the past, contact methods have been generally used to measure temperature with thermocouples. Nowadays, however, as the target size of research objects lessened to the nano-sized scale, it is impossible to apply the temperature-measurement methods with thermocouples to the small devices. Recently, for that reason, the LIF (Laser Induced Fluorescence) technique with fluorescence dye has been used in many research fields to obtain temperature values. Nevertheless, the LIF technique has difficulties in measuring surface temperature. Because in order to use the LIF method , fluorescence dye have to be dissolved into a solvent and the molecules in this solution are affected by flows. We present a non-invasive technique to measure temperature distribution in the nano-sized porous thin films by means of two-color laser-induced fluorescence (2-LIF) of rhodamine B. The fluorescence, induced by the green line of a Mercury lamp with the makeup of optical filters, is measured on two separated color bands. They can be selected for their strong difference in the temperature sensitivity of the fluorescence quantum yield. This technique allows absolute temperature measurements by determining the relative intensities on two adequate spectral bands of the same dye. The deviations of the fluorescence intensity were measured at several different temperatures, and the visualizations of the temperature field were performed by taking intensity distributions from the specimen at various temperature fields

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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    koamabayili/VECTRON-author-checklist: VECTRON author checklist

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    We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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