1,722,320 research outputs found
PLANNING FOR THE END OF THE CONSTRUCTION BOOM AND TRANSITION TO A NORMAL ECONOMY IN ACEH AND NIAS
Full text linkThere is a very real danger that the transition from the Construction Boom in 2009 to a rapidly growing sustainable normal economy in Aceh will not happen unless both preventive and effective constructive measures are implemented during the coming two years. Instead what could easily happen is a collapse into a deep recession caused by the economy of Aceh being uncompetitive relative to the rest of Indonesia because of its high costs and because in anticipation of this high cost economy situation insufficient investments were made by the private sector in 2006 and the coming two years.
BRR RNASeq collection - Cotton
No full textBlack root rot (BRR) disease of is an economically important disease of cotton and other crops, especially in cooler regions with short seasons. Symptoms include black discoloration of the roots, reduced number of lateral roots, and stunted or slow growth. The cultivated tetraploid species of cotton are susceptible. Resistance to BRR was identified in the G. arboreum accession BM13H and associated with reduced and restricted hyphae growth and sporulation. Transcriptome analysis indicated BM13H responded to infection at early time points 2- and three-days post inoculation, but that by day 5, few differentially expressed genes were observed between infected and uninfected roots. Inheritance of BM13H resistance to BRR was analysed in an F6 recombinant inbred population and was found to be conferred by a single dominant locus that was fine mapped to a region on chromosome 1, containing ten genes including five putative resistance-like genes
Aceh dan nias satu tahun setelah tsunami : Upaya pemulihan dan langkah ke depan
No full text+262hlm.;28c
Changes in total and functional bacterial genera following biochar application to planted soil
Full text linkBiochar has the recognized potential to sequester carbon, facilitate contaminant amelioration and enhance agricul-tural crop yield. Different types of biochar have different impacts on ecosystems, and those that are produced locally, relative to where they will be used, are considered more sustainable. It is important, therefore, to determine how the locally produced biochars affect total and functional microbial communities, especially in agronomic contexts. In this study we tested the hypotheses that biochar augmentation would: (1) increase plant yield; and (2) differentially affect total and functional microbial community composition and structure in bulk vs. rhizosphere (Trifolium pratense) soils. Triplicate randomised seedling cells of a 5% (w/w) mixture of sandy clay loam soil (26% clay, 21% silt and 53% sand), with/without locally-produced mixed broadleaf forestry biochar, and with/without 0.1 g clover seeds, were sampled destructively at 2-week intervals for 8 weeks post clover germination. Microbial DNA of bulk and T. pratense rhizos-phere soils were analysed with next-generation sequencing of the 16S rRNA gene. The results showed a statistically significant increase in plant biomass in response to biochar addition correlating to increased abundances of Armati-monadetes and Bacteroidetes specifically in the rhizosphere. Although no significant change in overall alpha diversity was observed, significant changes in abundance at the genus level were recorded particularly in the presence of biochar for a number of recognised nitrogen-fixing and plant growth-promoting bacteria, including those capable of indole acetic acid (IAA) production, plant disease suppression and degradation of toxic compounds. We conclude that although overall soil diversity may not be affected by biochar addition, key genera associated with soil health and nitrogen fixation, such as Pseudoxanthomonas, Variovorax, Pseudonocardia, Devosia, Lysobacter and Hydrogeno-phaga, increased and facilitated plant growth
Restoring the Final Frontier: Exosomal MicroRNA and Cutaneous Wound Repair
Full text linkoai:openjournals.ljmu.ac.uk:article/601Non-healing wounds present a major healthcare challenge associated with the ageing population, the rising incidence of diabetes and the obesity epidermic. Driven by the need to expand therapeutic options for the treatment of such wounds, a large body of evidence has emerged in recent years demonstrating that microRNAs (miRNAs) modulate various aspects of cutaneous wound healing through effects on diverse cell types, including keratinocytes, fibroblasts, endothelial cells and macrophages. However, clear translational pathways for non-invasive cutaneous delivery of miRNAs to facilitate wound repair have not yet been established. The recognition that miRNAs can be actively partitioned into extracellular vesicles (EVs)—exosomes, microvesicles and apoptotic bodies—has stimulated research into the regulation, function and translational exploitation of EV-derived miRNAs both as a novel mode of intercellular signalling and as a tool for miRNA transfer to cells for therapeutic purposes. In particular, because mesenchymal stem cells (MSCs) were found to support wound healing, there is much interest in the therapeutic potential of EVs, especially exosomes, derived from these cells. In this review, we survey some of the main mesenchymal stem cells (MSCs) for which exosomal miRNAs have been evaluated in the context of skin repair, including exosomes from adipose-derived MSCs, bone MSCs, amniotic MSCs and umbilical cord MSCs. Epithelial stem cell (EPSC)-derived exosomes are also considered, from keratinocytes and epidermal stem cells. The picture that emerges from studies on exosomes from various cell types reveal they share a limited set of exosomal miRNAs enhancing wound repair. We suggest a need for direct comparison of exosomal miRNA profiles from a range of MSCs and EPSCs. The ability of exogenous exosomal miRNAs to promote healing of chronic diabetic wounds also warrants further attention in order to more fully establish their therapeutic potential
ANALISIS DAMPAK PEMANFAATAN DANA BANTUAN BERUPA PIN.JAMAN BRR TERHADAP PENDAPATAN KOPERASI DI KOTA BANDA ACEH
Full text linkAdapun yang menjadi tujuan dalam penelitian ini adalah untuk mengetahui besarnya tingkat pendapatan koperasi sebelum dan setelah menerima bantuan dana dari BRR di Nanggroe Aceh Darussalam dan untuk mengetahui pengaruh bantuan dana BRR terhadap pendapatan koperasi di Nanggroe Aceh Darussalam.Data yang dikumpulkan dalam penelitian ini adalah data sekunder yang diperoleh dari intansi terkait khususnya pada Koperasi yang mendapakan bantuan BRR berjumlah sebanyak I5 koperasi, pengambilan sampel dilakukan dengan menggunakan metode "Stratified Random Sampling" informasi mengenai sample ini diperoleh dari kantor Departemen Koperasi, PNM dan Dekopinda Nanggroe Aceh Darussalam dan juga langsung dari koperasi yang diteliti.Dari hasil penelitian diperoleh bantuan modal BRR ternyata tidak berpengaruh terhadap pendapatan Koperasi di Nanggroe Aceh Darussalam. Dari I5 koperasi yang diteliti jumlah bantuan yang diterima koperasi dari BRR relatif bervariasi mulai dari Rp. 850.000.000 sampai Rp.1.400.000.000, dengan rata-rata pendapatan yang diperoleh koperasi sebelum mendapatkan bantuan sebesar Rp. 8.133.333 dan rata-rata pendapatan koperasi setelah mendapatkan bahtuan sebesar Rp. 11.933.333. Hal ini memberikan makna bahwa bantuan dana dari BRR ternyata hanya memberikan sedikit tambahan pendapatan koperasi di Nanggroe Aceh Darussalam Hasil penelitian dengan menggunakan analisis regresi diperoleh koefisien determinan (R) bernilai 0,003 atau 0,3 persen menunjukkan bahwa variabel bantuan dana BRR untuk pengembangan Koperasi berpengaruh terhadap pendapatan koperasi di nanggroe Aceh Darussalam sebesar 0,3 persen, sedangkan sisanya sebesar 99,7 persen adalah faktor-faktor lain yang tidak dimasukan dalam penelitian ini yang juga mempengaruhi pendapatan koperasi di Nanggroe Aceh Darussalam. Pembuktian yang dilakukan baik dengan menggunakan uji t maupun uji F menunjukkan bahwa variabel dana bantuan BRR untuk pengembangan Koperasi tidak berpengaruh signifikan terhadap pendapatan koperasi di Nanggroe Aceh Darussalam baik secara partial maupun secara keseluruhan.Diharapkan kepada BRR agar supaya kebijakan yang telah dilakukan terhadap pengembangan koperasi harus dikaji kembali, karena bantuan modal yang diberikan BRR ternyata tidak berpengruh signifikan terhadap penambahan pendapatan koperasi di Kota Banda Aceh. Dimasa yang akan datang BRR dalam mengalokasikan dana untuk koperasi harus memperhatikan kelayakan koperasi itu sendiri, supaya modal yang diberikan mampu memberikan tambahan pendapatan koperasi yang lebih besar
A novel peptide for microRNA delivery to medulloblastoma cells
Full text linkMedulloblastoma is a paediatric brain cancer categorised into various subtypes that have differing prognostic outcomesfor patients. As with other cancers, microRNAs have been implicated in medulloblastoma pathogenesis andthe loss of specific miRNAs appears to contribute to the disease. There is therefore an urgent need to developmiRNA-replacement therapies for medulloblastoma. However, methods for targeted delivery of small RNAs to medulloblastomacells have not been fully established. As a step towards tackling this challenge, we have developedself-assembling peptide nanoparticles for small RNA delivery to medulloblastoma cells. We generated an amphiphilicpeptide, TY-28, using solid-phase peptide synthesis and combined TY-28 with miR-124-3p. Analysis of the resultingcomplexes by electron microscopy and dynamic light scattering confirmed the formation of nanoparticles. The abilityof the NPs to penetrate cells was monitored by labelling the miRNA with a fluorescent dye. The NP:miRNA complexeswere readily internalised by group 3 medulloblastoma cells, and the accumulation of the complexes increased overtime. Levels of uptake were 6-fold higher at 24 hours compared to 4 hours in serum-free medium. The uptake of theNPs complexes by the cells did not differ in the presence and absence of serum, suggesting the presence of serumdid not affect complex stability. Our findings point to the translational potential of self-assembled NPs to delivery miRNAmimics to medulloblastoma cells
Regionalizing Recovery: Rehabilitation and Reconstruction Agency (BRR) Aceh-Nias BRR One Year Report Executive Summary
No full textFacile synthesis of CO2-selective membrane derived from butyl reclaimed rubber (BRR) for efficient CO2 separation
No full textButyl reclaimed rubber (BRR) was used as a novel precursor in the fabrication of a rubber membrane for CO2 separation. Fully de-vulcanized BRR without impurities was produced from waste tire by regeneration. The properties of BRR were evaluated via systematic investigation of a number of experimental variables, and the effects of these variables on the molecular structure and the CO2 separation performance of the membrane were assessed. The BRR-derived membrane was fabricated by simple solution-coating on an Al2O3 support. The effect of the fabrication parameters, such as the solvent type, polymer concentration (8–12 wt%), and curing temperature (room temperature to 100 °C). The results indicate that the CO2/N2 separation performance of the BRR-derived membrane prepared with a low concentration (<10 wt%) of coating dope in toluene solvent and cured at room temperature is competitive with that of membranes reported in previous studies due to the cis-isoprene structure. The CO2/N2 ideal selectivity of 11.6, coupled with a CO2 permeability of 86.9 Barrer, obtained with the 8RD-T25 membrane is comparable to that of a commercial rubber membrane. Furthermore, the use of BRR would decrease the use of non-renewable resources and aggregation of waste, with reduced environmental impact
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