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    Photosynthetic acclimation to elevated CO2 in poplar grown in glasshouse cabinets or in open top chambers depends on duration of exposure

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    The effects of elevated CO2 were studied on the photosynthetic gas exchange behaviour and leaf physiology of two contrasting poplar (Populus) hybrids grown and treated in open top chambers (OTCs in Antwerp, Belgium) and in closed glasshouse cabinets (GHCs in Sussex, UK). The CO2 concentrations used in the OTCs were ambient and ambient +350 µmol mol–1 while in the GHCs they were c. 360 µmol mol–1 versus 719 µmol mol–1. Measurements of photosynthetic gas exchange were made for euramerican and interamerican poplar hybrids in combination with measurements of dark respiration rate and Rubisco activity. Significant differences in the leaf anatomy and structure (leaf mass per area and chlorophyll content) were observed between the leaves grown in the OTCs and those grown in the GHCs. Elevated CO2 stimulated net photosynthesis in the poplar hybrids after 1 month in the GHCs and after 4 months in the OTCs, and there was no evidence of downward acclimation (or down-regulation) of photosynthesis when the plants in the two treatments were measured in their growth CO2 concentration. There was also no evidence of down-regulation of Rubisco activity and there were even examples of increases in Rubisco activity. Rubisco exerted a strong control over the light-saturated rate of photosynthesis, which was demonstrated by the close agreement between observed net photosynthetic rates and those that were predicted from Rubisco activities and Michaelis-Menten kinetics. After 17 months in elevated CO2 in the OTCs there was a significant loss of Rubisco activity for one of the hybrid clones, i.e. Beaupré, but not for clone Robusta. The effect of the CO2 measurement concentration (i.e. the short-term treatment effect) on net photosynthesis was always larger than the effect of the growth concentration in both the OTCs or GHCs (i.e. the longterm growth CO2 effect), with one exception. For the interamerican hybrid Beaupré dark respiration rates in the OTCs were not significantly affected by the elevated CO2 concentrations. The results suggest that for rapidly growing tree species, such as poplars, there is little evidence for downward acclimation of photosynthesis when plants are exposed to elevated CO2 for up to 4 months; longer term exposure reveals loss of Rubisco activity

    Elevated CO2 and hybrid poplar a detailed investigation of root and shoot growth and physiology of Populus euramericana, 'Primo'

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    Exposure of the hybrid poplar clone ‘Primo’ (Populus deltoidex × Populus nigra) to 580 ?l 1?1 carbon dioxide for just 68 days significantly (P ? 0.05) increased stem height by 13% compared with trees grown in ambient CO2 concentrations. The stem diameter was significantly (P ? 0.05) increased and both total biomass and woody stem biomass also showed higher values (38% and 31% increases respectively) in elevated CO2. Trees in elevated CO2 had more leaves and a greater total leaf area, whilst the specific leaf area was decreased in elevated CO2 on four out of five occasions and was significantly (P ? 0.05) lower after 68 days, an effect indicating that leaves were thicker and/or heavier. Rates of photosynthesis (A) measured after 49 and 67 days of exposure revealed that trees in the elevated CO2 treatment had lower values of A when measured at either 350 or 580 ?l 1?1 CO2. Sequential harvests at intervals during the study in which the root and shoot components were analysed separately allowed the construction of root:shoot ratios and allometric coefficients; there was no significant effect on the allometric coefficient and the root:shoot ratio was significantly increased on only one occasion. However, measurements of the ‘apparent’ root length suggested that root lengths were greater in the CO2 treatment. There was a significant increase in the number of fine root tips visible down the surface of specially designed rooting tubes (P ? 0.05), indicating more fine roots or an increase in fine root branching. The growth rates of individual fine or large roots over 24 h were unaffected, again suggesting that increases in biomass may be due to more root segments rather than longer individual roots. Root water relations were also examined and showed a tendency towards solute accumulation and increases in turgor pressure (P) and effective turgor (Pe) at times when root growth was stimulated, although these were not consistent. Cell wall plasticity of the tips of large roots was significantly (P ? 0.01) reduced in elevated CO2, possibly indicating a greater tendency to divert resources to the formation of root branches. The results of the study are discussed in the light of the possible consequences of changes in poplar growth and physiology for forestry practice in an increased CO2 environment

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Effects of elevated CO2 on cellular mechanisms, growth and development of trees with particular reference to hybrid poplar

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    Growth is often stimulated when C3 plants, including trees, are exposed to elevated CO2, although evidence from the literature suggests that the responsiveness of trees to CO2 varies, depending on species. This paper explores some of the cellular mechanisms which underlie increased growth, using both the authors' own data and information from the literature. Mechanisms include photosynthetic fixation of CO2 and the role of Rubisco, the link between carbon fixation and growth, in particular, how increased carbon is thought to influence the process of plant cell expansion and cell production and finally the consequences of cellular effects for the growth and development of whole plants. Data are presented for the growth and development of hybrid poplars in elevated CO2, following both field (open-top chambers) and laboratory experiments which suggest that this type of tree with indeterminate, rapid growth may be favoured by the CO2 concentrations of the next century

    Author Index

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    koamabayili/VECTRON-author-checklist: VECTRON author checklist

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    We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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