219 research outputs found
sj-pdf-1-ajs-10.1177_03635465211073148 – Supplemental material for Evaluation of the Effects of Transforming Growth Factor–Beta 3 (TGF-β3) Loaded Nanoparticles on Healing in a Rat Achilles Tendon Injury Model
No full textSupplemental material, sj-pdf-1-ajs-10.1177_03635465211073148 for Evaluation of the Effects of Transforming Growth Factor–Beta 3 (TGF-β3) Loaded Nanoparticles on Healing in a Rat Achilles Tendon Injury Model by Riza Mert Cetik, Samiye Yabanoglu Ciftci, Betul Arica, Ipek Baysal, Saadet Ozen Akarca Dizakar, Fatma Kubra Erbay Elibol, Ayse Gencer, Teyfik Demir and Mehmet Ayvaz in The American Journal of Sports Medicine</p
Counter-terrorism and the Prospects of Human Rights: Securitization of Difference and Dissent
No full textThis book offers a systematic analysis of how the interaction between language of security and language of rights produces policies which not only affect everyday functioning of democracy, but also redefine the understanding of sovereignty. Demirsu presents a rich theoretical framework and a novel methodological design, premised on a multi-method qualitative research that offers a comparative analysis of counter-terrorism and human rights in Turkey and the United Kingdom. While Part I offers an analysis of the evolution of these two key policy-areas in relation to each other, Part II presents the findings of the frame analysis of parliamentary debates, both concluding by mapping out cross-cutting patterns in these two cases. As a result, the author demonstrates in detail how discourse and policy-making are mutually constitutive from a comparative angle
Book review: French-language road cinema: borders, diasporas, migration and 'New Europe' by Michael Gott
No full textAs road movies have become more prominent in European cinema, in French-Language Road Cinema: Borders, Diasporas, Migration and ‘New Europe’ author Michael Gott analyses a corpus of French-language films that utilise the road to explore the complexity of mobility, borders and identity in contemporary Europe. Ipek Celik Rappas welcomes this as a thoughtful, timely and exciting book that will be an indispensable guide for researchers in European film and mobility studies
Correction: Experimental investigation of the effects of different quaternary elements (Ti, V, Nb, Ga, and Hf) on the thermal and magnetic properties of CuAlNi shape memory alloy (Journal of Materials Research, (2022), 37, 14, (2271-2281), 10.1557/s43578-022-00625-y)
No full textThis article was updated to correct İpek A. K. Arpa’s name. © The Author(s), under exclusive licence to The MaterialsResearch Society 2022
Alzheimer Hastalığı Tedavisinde Kullanılmak Üzere Donepezil Yüklü Plga-B-Peg Nanopartiküllerinin Hazırlanması ve Beyne Hedeflendirilmesi
No full textAlzheimer's disease (AD) is an irreversible and progressive neurodegenerative
disease, which is caused by irreversible loss of neurons in the hippocampus and
cortex regions of the brain. Although the molecular mechanism of the disease is still
unclear, the deposition of the amyloid beta (Aβ) proteins (senile plaques) in the
extracellular synaptic spaces of the neocortex is suggested to play a major role in
progress of AD. Cholinesterase inhibitors are compounds that are used for
symptomatic treatment of neuron transmission improvement in AD. Donepezil is a
reversible and non-competitive cholinesterase inhibitor that preferably inhibits
acetylcholinesterase compared to butyrylcholinesterase and is clinically used for
treatment of AD. In the frame of this thesis, we developed donepezil loaded poli
(laktic-co-glycolic acid)-block-poli (ethylen glycol) [PLGA-b-PEG] nanoparticles to
increase efficiency of treatment in AD and to reduced the side effects of donepezil
nanoparticles were directly targeted to the Aβ fibriles. By targeting donepezil to the
brain in sufficient concentration we destabilized Aβ fibrile formation and minimized
its tissue distrubition. Furthermore by setting up a blood brain barrier model in vitro,
using microvascular endothelial cells and astrocytes we determined that this prepared
nanoparticles passed across the blood brain barrier. In this set up we also evaluated
the protective effect of donepezil on astrocytes in neuroinflammation by assessing
cytokine expressions both in protein and gene levels. According to these results for
inflammation cytokines IL-1β, IL-6, GM-CSF, TGF-β, MCP-1 and TNF-α, increase
in both expression and protein levels was detected on astrocytes which were
incubated with amyloid fibrils. After free donepezil and donepezil loaded
nanoparticle administration to these stimulated astrocytes a significant decreased in
both gene expression and protein levels were detected for IL-1β, IL-6, GM-CSF and
TNF-α, whereas there were no significant changes observed for TGF-β and MCP-1.
In vivo Alzheimer model was created using intracerebroventricular injection of
Aβ(25-35) peptide. After the treatment period brain tissues of rats were removed and
homogenized. Following the homogenization of the brain tissues;
acetylcholinesterase activities were determined in each sample. According to the
results acetylcholinesterase activity of Alzheimer model rats achieved with icv
Aβ(25-35) injection were found to be significantly increased. Effect of donepezil
loaded nanoparticles in this animal model were found to be more effective than free
donepezil and significantly inhibited the acetylcholinesterase activity. Based on these
results, ability of donepezil loaded nanoparticles to inhibit acetylcholinesterase
activity is stronger than high dose free donepezil. The reason for this can be
attributed to the controlled release of donepezil, longer duration of action and
successful targetting of nanoparticles.Alzheimer hastalığı (AH), beynin hipokampüs ve korteks
bölgelerinde geri dönüşümsüz nöron kaybı ile ortaya çıkan nörodejeneratif bir
hastalıktır. Hastalığın moleküler mekanizması açık olmamakla birlikte AH’nda,
neokorteksin ekstraselüler sinaptik boşluklarında amiloid beta (Aβ) proteinlerinin
birikerek plaklar oluşturduğu ve nöron dejenerasyonuna yol açtığı öne sürülmektedir.
Kolinesteraz inhibitörleri, AH’nda bozulan sinir iletimini düzeltmeyi amaçlayan
semptomatik tedaviye yönelik bileşiklerdir. Donepezil, AH’nda klinik kullanımı
olan, tersinir ve non-kompetetif bir kolinesteraz inhibitörüdür ve bütirilkolinesteraza
kıyasla asetilkolinesterazı (AKoE’ı) daha özgül olarak inhibe eder. Bu tez
kapsamında AH tedavisine yönelik olarak; donepezilin yan etkilerini azaltmak ve
tedavi etkinliğini artırmak için donepezil yüklü poli(laktik-ko-glikolik asit)-blokpoli(
etilen glikol) [PLGA-b-PEG] nanopartikülleri geliştirilmiş ve bu nanopartiküller
doğrudan beyindeki Aβ fibrillerine hedeflenmiştir. Donepezil Aβ fibrillerine
yönlendirildiğinden doku dağılımı minimize edilmiş, doğrudan Aβ fibrillerini
destabilize edecek konsantrasyonda beyne ulaşması sağlanmıştır. Ayrıca insan beyin
mikrovasküler endotel hücreleri ve astrositleri kullanılarak hücre kültürü ortamında
kan beyin bariyeri modeli oluşturulmuş hazırlanan nanopartiküllerin kan beyin
bariyerini geçtiği tespit edilmiştir; donepezilin koruyucu etkisini değerlendirebilmek
adına da astrositlerden salınan ve nöroenflamatuar süreçte rol oynayan sitokinlerin
ekspresyonları protein ve gen düzeyinde belirlenmiştir. Bu sonuçlara göre fibrillerle
inkübe edilen astrositlerde enflamasyona ait sitokinlerden IL-1β, IL-6, GM-CSF,
TGF-β, MCP-1 ve TNF-α için hem gen hem protein düzeyinde ekspresyon artışı
tespit edilmiştir. Donepezil ve donepezil yüklü nanopartikül ile uyarılma sonucunda
ise bu sitokinlerden IL-1β, IL-6, GM-CSF ve TNF-α düzeylerinde anlamlı azalma
hem gen ekspresyon düzeylerinde hem de protein düzeylerinde tespit edilirken TGF-
β, MCP-1 sitokinleri için ise anlamlı değişiklik saptanmamıştır. Aβ(25-35) peptidinin
intraserebroventriküler enjeksiyonu ile Alzheimer benzeri model oluşturulmuştur.
Tedavi sürecinin ardından farmakolojik deneyler tamamlandıktan sonra sıçanların
beyin dokusu çıkarılarak homojenize edilmiştir. Hayvan beyin dokularının
homojenize edilmesini takiben homojenatlarda asetilkolinesteraz aktivite tayini
yapılmıştır. Bu sonuçlara göre Aβ(25-35) fibrilleri kullanılarak AH modeli
oluşturulmuş olan sıçan grubunda aktivitenin anlamlı ölçüde arttığı saptanmıştır.
Donepezil yüklü nanopartiküllerin donepezilden daha etkin davranarak aktiviteyi
anlamlı şekilde inhibe ettiği saptanmıştır. Bu sonuçlardan yola çıkarak donepezil
yüklü nanopartiküllerin AKoE aktivitesini inhibe etme güçlerinin donepezile oranla
daha fazla olduğu söylenebilir. Bu da nanopartiküllerden donepezilin kontrollü
olarak salındığının, etki süresinin daha uzun olmasının ve hedeflemenin başarılı
olmasının bir sonucu olarak değerlendirilebilir
Selektif MAO-B inhibitörü selejilin yüklü PLGA-b-PEG nanopartiküllerin hazırlanması ve beta-amiloid fibrillerle etkileşiminin incelenmesi
No full textLA CITTÀ DELL'ODIO E I SUOI ANTICORPI: IDENTITÀ CONTRASTANTI LEGATE AL LUOGO E LA TERRITORIALIZZAZIONE DELL'APPARTENENZA A VERONA
No full textQuesta ricerca mira a mostrare il potenziale euristico dello ‘spazio sociale’ nell’ambito degli studi su movimenti sociali con l’utilizzo delle categorie analitiche di ‘identity of place’ (identità del luogo) e di ‘place-based identities’ (identità legate al luogo) per un’analisi comparativa dei movimenti antagonisti nella città di Verona, che promuovono costruzioni concorrenti di cittadinanza urbana (appartenenza quotidiana alla città). Mentre la città di Verona viene riconosciuta come la fortezza dell’estrema destra nella penisola italiana e ‘città modello’ per la rete transnazionale degli attori identitari, essa dimostra di possedere anche delle potenzialità per movimenti dal basso che lavorano per la realizzazione di un’appartenenza inclusiva e pluralistica nella vita quotidiana della città. L’analisi basata su 3 anni di ricerca sul campo svolta con metodi dell’osservazione partecipante, etnografia online, interviste approfondite, ricerca d’archivio e metodi visuali; e mostra come l’identità collettiva e l’azione collettiva degli attori sociali sono influenzate dal loro rapporto con lo spazio in cui operano. Da un lato, un’alleanza dei identitarismo sovranista è unita intorno a un’identità territoriale escludente che si incarna nell’introspettivo paesaggio turistico del centro storico, e dall’altro, un ‘assemblage’ di gruppi progressisti autonominatesi ‘gli anticorpi dell’odio’ si è formato attraverso l’identità territoriale espansiva che ha contribuito a realizzare nel estroversivo interculturale quartiere di Veronetta. Questi attori sono autori di continue lotte quotidiane negli spazi urbani condivisi attraverso pratiche di territorializzazione, ‘placemaking’ trasformativo, e rappresentazioni alternative dello spazio che non solo funzionano a inscrivere la loro visione della cittadinanza urbana (city-zenship) su tale spazio, segnando così i confini cognitivi di inclusione e esclusione, ma volgono anche alla connessione con lotte simili in contesti diversi.This study is an attempt to show the heuristic potential of social space in the field of social movement studies with the utilization of the analytical categories ‘identity of place’ and ‘place-based identities’ for a comparative analysis of antagonistic movements in the city of Verona, promoting competing constructions of city-zenship (belonging and everyday membership to the city). While the city of Verona has come to the fore as the fortress of the far-right in the Italian peninsula and a model city for the transnational network of identitarian actors, it also demonstrates grassroots potentials for the realization of an inclusive and pluralist belonging in the everyday city life. The analysis premised on 3 years of fieldwork that included participant observation, online ethnography, in-depth interviews, archival research, and visual methods, demonstrates the ways in which collective identity and collective action of social actors are shaped by their relationship to the space in which they operate. On the one hand, an alliance of sovereignist identitarianism is united in an exclusionary territorial identity that is embodied in the inward-looking touristscape of the historical center, and on the other hand, an assemblage of progressive groups referred to as the ‘antibodies of hate’ is formed through the expansive place-based identity that they have helped establish in the outward-looking neighborhood of Veronetta. These actors author everyday struggles in shared urban spaces through practices of territorialization, transformative placemaking, and alternative representations of space that not only work to inscribe their vision of city-zenship upon such space, thereby marking cognitive boundaries of inclusion and exclusion, but also connect them with similar struggles in different settings
Alzheimer hastalığı tedavisinde kullanılmak üzere donepezil yüklü PLGA-b-PEG nanopartiküllerinin hazırlanması ve beyne hedeflendirilmesi
No full textEVALUATION OF INTERPERSONAL PROBLEM SOLVING AND SOCIAL SKILLS AND PEER RELATIONS IN EARLY CHILDHOOD
No full textBu araştırmanın amacı sosyal beceri puanı en düşük ve en yüksek olan okul öncesi dönem çocuklarının kişilerarası problemçözme becerilerinin ve arkadaşlık ilişkilerinin değerlendirilmesidir. Bu amaç doğrultusunda araştırmada, nitel ve nicel verilerinharmanlandığı karma yöntem kullanılmıştır. Çalışma grubunu okul öncesi eğitim almakta olan 17’si 48-60 ay, 92’si ise 60-72ay olan 57 kız ve 52 erkek çocuk olmak üzere toplam 109 çocuk oluşturmaktadır. Araştırmanın sonucunda çocukların sosyalbecerisi ile sosyal olmayan akran çözümleri arasında negatif yönde anlamlı ilişki bulunmuştur. Sosyal becerisi yüksek olançocukların akran ilişkileri incelendiğinde birlikte oyun, akranlarını uyararak uygun davranışa yönlendirme, liderlik,akranlarıyla iletişim kurma, akranlarına yardım etme, nezaket sözcüklerini kullanma, sabretme/bekleme, akranlarıyla fizikselyakınlık kurma, davranışların nedenlerini sorgulama, akranları tarafından reddedilme/tercih edilmeme, akranlarını şikâyetetme, akranlarıyla olumsuz sözel iletişim ve akranlarıyla alay etme temaları ortaya çıkmıştır. Sosyal becerisi düşük olançocukların akran ilişkileri incelendiğinde yalnız kalma isteği, akranlarını fiziksel olarak zorlama/müdahale, akranları tarafındanreddedilme/tercih edilmeme, akranlarını şikâyet etme, otoriteden korkma, arkadaşlığı kaybetme korkusu, akranlarıyla olumsuzsözel iletişim, akranlarıyla sözel iletişim kurmadan oyun oynama ve akranlarıyla iletişim kurma temaları ortaya çıkmıştır.The aim of this study is to evaluate the interpersonal problem-solving skills and friendship relationships of pre-school children with the lowest and highest social skills score. For this purpose, a mixed method was used which combined qualitative and quantitative data. The study group consisted of 109 children, 17 of whom were 48-60 months old and 92 of whom were 60-72 months (57 girls and 52 boys). Based on the findings, there exists a negative and significant relationship between children’s social skills and non-social peer solutions. When the peer relations of the children with high social skills are examined, it was found that those children play together, stimulate their peers, show leadership behaviors, communicate with and help their peers, use the words of courtesy, show patience, wait for their turn, show physical closeness with their peers, question the causes of behaviors, rejection by their peers, complain about their peers, communicate negatively with peers and mockery with their peers. When the peer relations of the children with low social skills are examined, the following behaviors have emerged: desire to be alone, show physical coercion/intervention/rejection towards their peers, complain about their peers, show fear towards authority and losing friendship, communicate negatively with their peers, play with their peers without any verbal communication
Evaluation of the Effect of Hydatid Cyst Fluid on the Apoptosis Pathway in BEAS-2B and A549 Cell Lines
No full textSome of the pathogenic microorganisms have been associated with cancer due to the activation of cancer precursors in the host because of the inflammatory processes. Additionally, some other pathogens prevents the tumor formation by creating an anti-neoplastic immune response which has been reported to stop the development of cancer. Cystic echinococcosis (CE) or cyst hydatid disease (CHD) is a zoonotic infection caused by the larval form of Echinococcus granulosus sensu lato in humans. It has been reported that there is a negative correlation between E.granulosus infection and cancer and it has been suggested that direct and/or indirect E.granulosus infection may have an anti-cancer effect. However, the molecular mechanisms of this effect still remains unclear. The aim of this study was to evaluate the effect of hydatid cyst fluid administration on cell proliferation and expression of some apoptotic genes (BCL-2, p53 and BAX) in human healthy lung epithelial (BEAS-2B) and human lung adenocarcinoma (A549) cell lines and understanding the molecular mechanism underlying the possible anti-cancer action mechanism of hydatid cyst fluid. In order to evaluate the effect of hydatid cyst fluid on cell proliferation and apoptotic gene expression, cell proliferation assay (XTT) and real-time polymerase chain reaction (Rt-PCR) were performed, respectively. After the application of hydatid cyst fluid, there was no change in the cell proliferation. A statistically significant decrease in BCL-2 gene expression (> 90 fold) and an increase in p53 gene expression (> 1.2 fold) were found. No significant change in BAX gene expression was detected. In this study, it was found that the application of hydatid cyst fluid did not directly cause cell death but it has shown for the first time to sensitize the A549 cell line, which is resistant to apoptosisand shed light on the possible mechanism of hydatid cyst fluid in the apoptotic pathway
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