14,457 research outputs found
First person - Marcel Nowak and Benjamin Suenkel
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Marcel Nowak and Benjamin Suenkel are co-first authors on 'DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy', published in JCS. Marcel subsequently worked as a Product Manager for a life science company. Benjamin is a postdoc in the lab of Thomas Sommer at Max-Delbrück-Center for Molecular Medicine (MDC), Berlin-Buch, Germany, investigating protein biochemistry and quality control
Professor Jadwiga Nowak – multiple thoughts, multiple portraits
No full textW artykule, autor przypomniał sylwetkę Jadwigi Nowak, Profesora Wydziału Pedagogicznego Uniwersytetu Warszawskiego, pierwszego kierownika Katedry Pedagogiki Dorosłych. Opisując sylwetkę uczonej wyeksponowano zwłaszcza te wątki, które w polskiej debacie andragogicznej zainicjowała Profesor Jadwiga Nowak m.in.; znaczenie twórczości, rolę pracy zawodowej w rozwoju osobistym dorosłego, czy miejsce jakie zajmuje w koncepcjach edukacji całożyciowej problematyka wartości. Przypomniano inicjatywy Pani Profesor promujące rozwój środowiska polskich andragogów oraz ważniejsze funkcje jakie pełniła podejmując różnorodne inicja-tywy wydawnicze. Podkreślono niepowtarzalność osobowości uczonej.The subject of this article is Jadwiga Nowak, a well known Polish professor from the University of Warsaw's Faculty of Pedagogy and the first Head of the Faculty of Adult Education. In describing this researcher, certain themes are highlighted, especially those initiated by prof. Jadwiga Nowak i.e. the value of creativity, the role of work in an adult's personal development and the meaning of values in lifelong learning. The author describes many initiatives which were inspired by Jadwiga Nowak, especially the significant value of creativity, the role of the workplace in personal development and the meaning of value in lifespan edu-cation. Jadwiga Nowak‟s promotion of Polish andragogues, her various publishing initia-tives and her unique personality are also recalled in the article
Heteroplasmy selection and intermolecular mtDNA recombination in humans - analysis in a cell model
No full textBrief project description:As part of this project, human cybrid cell lines carrying a mixture of neutral mtDNA haplotypes differing in multiple nucleotide positions but not harboring pathogenic variants were generated. The aim of this study was to investigate the presence of heteroplasmy selection and intermolecular mtDNA recombination in humans in vitro.To generate cybrids containing a mixture of two mtDNA haplotypes, human 143B.TK- cells devoid of mtDNA (ρ0) were fused with enucleated cells serving as mitochondrial donors (cytoplasts). Six combinations of four mtDNA haplotypes (A, B, C, and D) were used, varying in genetic distance measured by the number of nucleotide differences. The nucleotide sequences of the studied mtDNA haplotypes were deposited in the GenBank database under accession numbers PQ468425 (A), PQ468426 (B), PQ468427 (C), and PQ468428 (D).Individual cell clones were screened for the presence and level of mtDNA heteroplasmy. mtDNA heteroplasmy was monitored over successive passages during cell culture to assess the occurrence of selection, its direction, and rate. Preliminary assessment was performed using Sanger sequencing of a selected mtDNA region (a fragment of the non-coding control region characterized by the highest variability). Detailed analysis of heteroplasmic cybrid lines was subsequently performed using high-throughput whole mtDNA sequencing using a method developed and implemented in our group's research on the MiSeq platform (Illumina) (https://doi.org/10.1016/j.exer.2018.10.004). The occurrence of intermolecular recombination in mtDNA in human cell culture in vitro was assessed by direct sequencing of native mtDNA molecules using Nanopore sequencing technology.The dataset consists of the following zip archives:Sanger_data.zip – DNA traces/chromatograms from Sanger sequencing (*.ab1) of selected mtDNA region for individual cybrid cell clones at different passages. The files can be opened using Sequencher software or any other similar bioinformatics software for Sanger data analysis.Illumina_FASTQ_files.zip – raw sequencing data as FASTQ files (*.fastq.gz) from next-generation sequencing (NGS) of mtDNA from heteroplasmic cybrid cell lines at different cell passage points. The files can be opened using CLC Genomics Workbench software, any other similar bioinformatics software, or open-source bioinformatics tools for NGS data analysis.Illumina_BAM_files.zip – mapped reads as BAM files and their corresponding index files (*.bam and *.bam.bai) generated from the bioinformatics analysis and mapping of mtDNA NGS data to the human mtDNA reference genome, rCRS (NC_012920). The files can be opened using CLC Genomics Workbench software, any other similar bioinformatics software, or open-source bioinformatics tools for NGS data analysis.Illumina_variant_lists.zip – Lists of mtDNA variants (SNVs and indels) identified from the bioinformatics analysis of mtDNA NGS data (*.xlsx). The files can be opened with MS Excel or any other spreadsheet software.ONT_data.zip – raw sequencing data as FASTQ files (*.fastq.gz) of long reads from direct sequencing of native mtDNA molecules using Oxford Nanopore Technology (ONT). The files can be opened using CLC Genomics Workbench software, any other similar bioinformatics software, or open-source bioinformatics tools for NGS/ONT data analysis.File naming instructions:Sanger files: e.g. F1_A_B_6_P0_M13_uni_21.ab1F1_ – Fusion experiment number (1–3)A_B_ – mtDNA haplotypes (A–D) = cybrid cell line6_ – Clone numberA3_ – Daughter line number (A3, E4, or F6)P0_ – Passage numberM13_uni_21 – Universal sequencing primer for Sanger sequencingIllumina files: e.g. 16_A_C_5_A3_P3_S17_L001_R1_001.fastq.gz, 16_A_C_5_A3_P3_S17_L001.bam, 16_A_C_5_A3_P3_S17_L001.bam.bai, 16_A_C_5_A3_P3_S17_L001.xlsx16_ – LR-PCR amplicon 16 kb or AB (for details, please see https://doi.org/10.1016/j.exer.2018.10.004)A_C_ – mtDNA haplotypes (A–D) = cybrid cell line5_ – Clone numberA3_ – Daughter line number (A3, E4, or F6)P3_ – Passage numberS17_ – Sample number based on the order of samples in the sample sheetL001_ – Flow cell lane numberR1_ – Read number in paired-end sequencing run (1 or 2)001 – Constant segment in FASTQ files.Nanopore files: e.g. mtDNA_A_C_5_A3_ont_2kb_q10.fastq.gzmtDNA_ – Mitochondrial DNAA_C_ – mtDNA haplotypes (A–D) = cybrid cell line5_ – Clone numberA3_ – Daughter line number (A3, E4, or F6)ont_ – Oxford Nanopore Technology2kb_ – Data were filtered to include reads longer than 2 kbq10_ – Data were filtered to include reads with a quality score greater than 10.</ul
The surface impedance of metals at 24,000 Mc/sec
No full text"May 27, 1949."Bibliography: p. [146].Army Signal Corps Contract No. W-36-039 sc-32037, Project no. 102B. Dept. of the Army Project No. 3-99-10-022.[by] W.B. Nowak
The Stone Age Man in the Caves of the Tatra Mountains - pictures
No full text"This research was funded in whole or in part by National Science Center UMO-2021/41/B/HS3/03217. For the purpose of Open Access, the author has applied a CC-BY public copyright licence to any Author Accepted Manuscript (AAM) version arising from this submission.” The dataset is the result of activities related to the implementation of the project. It presents photos documenting the archaeological excavation in the Obłazkowa cave in the Tatra Mountains, as well as illustrations of Paleolithic stone artifacts from the Huciva cave in the Tatra Mountains in Slovakia
Juliusz Nowak-Dłużewski's School. Historical-Literary Connections
No full textJuliusz Nowak-Dłużewski (31.03.1893, Goszcza – 19.04.1972, Warszawa), a literary scholar and historian, a teacher in secondary schools in Kielce and Warszawa. He did Polish Studies and his PhD in 1932 at the Jagiellonian University in the care of his promoter, Professor Ignacy Chrzanowski. During World War II he lectured at Kielce branch of Polish Studies of University of Western Lands. After the war he habilitated in Toruń on the basis of his monograph dedicated to priest Stanisław Konarski, afterwards, he took a job as a substitute for a Professor at Catholic University of Lublin and as an associate professor at University of Warsaw. His teaching and pedagogic service bore fruit in 1938 in the form of the Gold Cross of Merits. Post-war frameworks of reconstruction and the effort of the preservation from destruction of post-courtly collections from the position of the director of the National Museum in Kielce resulted in the Order of Polonia Restituta (1963). Finally, Nowak-Dłużewski’s research and development works met with recognition from the jury of the Włodzimierz Pietrzak Literary Award two times (1964, 1967).
The memoir dedicated to Professor J. Nowak-Dłużewski develops from his student’s conviction about the perfection of his didactic and educational mission performed by him with devotion during the difficult times when Communism was on the rampage. We experienced Professor’s special care and foresight in the last decade of his life (1962-1972), when he resolved to “remake us into angels” by destining us for creative work in conditions close to monastic closure – in the team of a PhD seminary. A doubtless protagonist of the given text is our Master, Professor Juliusz Nowak-Dłużewski. For this reason, the references include only articles and utterances of particular people with the intention of underlining our interpersonal master-pupils bond. To my mind, this aspect appears to excuse the author in the eyes of Colleagues as well as Readers
Leszek Nowak – obituary
No full textOn 20th of October 2009 died Leszek Nowak, one of the most creative and original Polish philosophers. Born on 7th of January 1943, Nowak studied law (Adam Mickiewicz University, Poznań) and philosophy (Warsaw University). He lived to philosophize. It is not a conventional phrase but the true description of his activities. He devoted his live to philosophy. He got professor’s title in 1976, at the age of 33. At that time he was the youngest professor in Poland and the author of the methodological conception – the idealizational theory of science. His theory was inspired by ideas he found in Marx’s writings. He made them explicit and precise by using the language of contemporary logical philosophy. The result was the theory that offered a new, detailed and systematic picture of science [10], [18]. Leszek Nowak admired Marx’s ideas and planned to reconstruct Marx’s entire philosophical system. It soon occurred to him that Marx’s social philosophy was unable to account for the functioning of the societies of so-called real socialism. Nowak retained Marx’s materialism but rejected the narrow, economic view of society. In 1977 he started to work on a new, generalized social theory, which he called the non-Marxian historical materialism [12]. In this theory real socialism occurs as the most oppressive system in the history of the hitherto known societies. One has to be bold or naïve to work on such a theory in the country of real socialism. Leszek Nowak was aware of the risk but he did not decide to accept intellectual compromise and in 1979 disseminated the typescript of his book on real socialism. During the time of Solidarność movement he spent all energy to educate union members and to reveal the oppressive nature of socialism. He was interned on 13th of December 1981 and spent a year in jail. In 1985 he was expelled from the university and in 1989 his professorship was reinstated. Extremely hard work, engagement in the Solidarność movement, and protests during internment seriously undermined his health. In the last years Leszek Nowak was not able to teach but he painstakingly worked on his new love – metaphysics. Results of his research were published in Polish in three volumes [16], [19], [20]. In our view his conception represents a non-standard approach to metaphysical problems. In Polish philosophy Nowak’s metaphysics can be only confronted with Roman Ingarden’s The controversy over the existence of the world. It is our firm conviction that Leszek Nowak’s place in Polish philosophy in the second half of the twentieth century is defined by the following qualities: a bold search for new and original idea, laborious work to present it in a systematic way, readiness to defend it against petrifying tendencies be they scientific, political, religious or ideological
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