2,179 research outputs found
Un spécimen unique de l'art tahitien
No full textDanielsson B. Un spécimen unique de l'art tahitien. In: Journal de la Société des océanistes, tome 20, 1964. pp. 88-89
Danielsson (B.) et O'Reilly (P.) : Gauguin, journaliste à Tahiti et ses articles des « Guêpes »
No full textFaivre Jean-Paul. Danielsson (B.) et O'Reilly (P.) : Gauguin, journaliste à Tahiti et ses articles des « Guêpes ». In: Revue française d'histoire d'outre-mer, tome 57, n°208, 3e trimestre 1970. pp. 355-357
Nuclear medicine imaging of breast cancer and regional lymph nodes
No full textThe aim of this thesis was to explore the role of nuclear medicine in diagnosis of primary breast carcinoma and regional lymph node involvement. The introduction includes a review over nuclear medicine techniques currently under investigation.Methods: In paper I, II, III and V patients were preoperatively investigated by scintimammography with 99m-Tc-Sestamibi. Ninety-six patients were imaged in prone position as a standard. All patients were routinely assessed by triple diagnosis (TD), i.e., physical investigation, mammography and fine-needle aspiration cytology. The results of TD had led to the decision that the patient was to be operated upon. Twenty-six patients were additionally imaged with single photon emission tomography (SPECT). Paper I, II and V evaluated Sestamibi uptake in breasts and paper III uptake in axillary lymph nodes. In paper IV detection of sentinel node (SN) in patients with breast carcinoma was studied. Lymphoscintigraphy with 99m-Tc-Nanocolloid, preoperative injection of Patent blue dye and peroperative use of gamma probe were applied on 75 patients.Results and Implications: Scintimammography with 99m-Tc-Sestamibi showed a sensitivity of 84 % and specificity of 74 % (Paper II and V), which was not improved by the additional use of SPECT (Paper 1). The sensitivity of scintimammography in the detection of primary breast lesion depends on tumour size, site and histological features. Furthermore, also benign lesions showed increased Sestamibi uptake, which lead to false- positive findings. The method had unsatisfactory diagnostic accuracy in the detection of axillary lymph node metastases (Paper III). Complementary use of scintimammography to TD improved the sensitivity in diagnosing cancers, and was specialty valuable in patients with mammographically dense breast parenchyma (Paper V). The combined use of preoperative lymphoscintigraphy, injection of blue dye and the peroperatively use of a gamma probe resulted in a detection rate of SN of 92% in all patients. SN correctly predicted the axillary status in 96% of the cases and might therefore be a potential concurrent to axillary lymph node dissection (Paper IV).Conclusions: Scintimammography can be recommended as a complementary method to TD, especially in patients with mammographically dense breasts. Biopsy of SN can be used instead of axillary lymph node dissection in selected patients.List of scientific papersI. Danielsson R, Bone B, Agren B, Svensson L, Aspelin P (1999). "Comparison of planar and SPECT scintimammography with 99mTc-sestamibi in the diagnosis of breast carcinoma. " Acta Radiol 40(2): 176-80 https://pubmed.ncbi.nlm.nih.gov/10080730II. Danielsson R, Bone B, Gad A, Sylvan M, Aspelin P (1999). "Sensitivity and specificity of planar scintimammography with 99mTc-sestamibi. " Acta Radiol 40(4): 394-9 https://pubmed.ncbi.nlm.nih.gov/10394867III. Danielsson R, Bone B, Perbeck L, Aspelin P (1999). "Evaluation of planar scintimammography with 99mTc-MIBI in the detection of axillary lymph node metastases of breast carcinoma. " Acta Radiol 40(5): 491-5 https://pubmed.ncbi.nlm.nih.gov/10485237IV. Frisell J, Bergqvist L, Liljegren G, Thorn M, Damm S, Rydman H, Danielsson R (2001). "Sentinel node in breast cancer--a Swedish pilot study of 75 patients. " Eur J Surg 167(3): 179-83 (In Print) https://pubmed.ncbi.nlm.nih.gov/11316401V. Danielsson R, Reihner E, Grabowska A, Bone B (2000). "The role of scintimammography with 99mTc-sestamibi as a complementary diagnostic technique in the detection of breast cancer" Acta Radiol 41(5): 441-5 https://pubmed.ncbi.nlm.nih.gov/11016763</p
Miniaturized Thermal Biosensors
No full textA summary of our efforts to develop miniaturized biosensors based on the enzyme thermistor is presented. Three constructions are described. The work focuses on the measurement of glucose in whole blood.The first biosensor was used to analyze concentrated and tenfold diluted blood samples. The glucose concentration obtained using this construction correlated well with the reference method.Using the second biosensor, the response time could be reduced from 3 minutes down to 30 seconds using a sample volume of 1 μl. The relative standard deviation for 100 blood samples (3.8 tnM) was 3.7%.Micromachining technology was used to construct the third thermal biosensor on a silicon chip. Enzymes were immobilized directly onto the 30 parallel flow channels. Injection of 200 samples containing 10 mM hydrogen peroxide gave a relative standard deviation of 3%
Sustainable development of nearshore areas. Risks and prospects in coastal zone management. Coastal meeting 2013. Eds: Rydell, B, Danielsson, P
No full text(I) Tema 1: Risk och sårbarhet i kustområden: (1) Cooper, A: Determining coastal vulnerability. Multi-scale Coastal Vulnerability index developed for Northern Ireland; (2) Danielsson, P: Sårbarhet för strandnära områden. Nationell kartering med erosionsindex; (3) Morf, A: Klimatanpassning i Ystads kommun. Riskuppfattning och hanteringsberedskap; (II) Tema 2: Kustplanering – praktikfall: (1) Nyberg, J: Marin kartering längs Skånes kust. Projket skånestrand, katering av marina områden; (2) Borell, C: Erosion och kustskydd i Kävlinge kommun. Praktikfall; (3) Rydell, B: SGI 10 år som samordnare av stranderosion; (III) Tema 3: Planeringsunderlag: (1) Cooper, A: Coastal development in the British Isles. Contemporary approaches and issues in coastal development in the British Isles; (2) Askman, P: Multifunktionell kustplanering. Ett regionalt utvecklingsperspektiv; (3) Lindeberg, G: Användning av satellitbilder och Nationell höjdmodell; (4) Wikström, S: Laserskanning och flygbilder för kartering av kustzonen; (5) Alm, E: Vad kan vi tillåta med hänsyn till naturen? Tillståndsfrågor vid kustskydd; (6) Swenson, D: Coastal municipality master plan template; (7) Hanson, H: En exposé över skånska kustproblem; (IV) Senaste nytt: (1) Danielsson, P: På gång på SGI; (2) Nisser-Larsson, M: Nationella plattformen - naturolyckor; (3) Larson, M: På gång på LTH; (4) Sjöström, Å: Liten klimatuppdaterin
Sustainable development of nearshore areas. Risks and prospects in coastal zone management. Coastal meeting 2013. Eds: Rydell, B, Danielsson, P
No full text(I) Tema 1: Risk och sårbarhet i kustområden: (1) Cooper, A: Determining coastal vulnerability. Multi-scale Coastal Vulnerability index developed for Northern Ireland; (2) Danielsson, P: Sårbarhet för strandnära områden. Nationell kartering med erosionsindex; (3) Morf, A: Klimatanpassning i Ystads kommun. Riskuppfattning och hanteringsberedskap; (II) Tema 2: Kustplanering – praktikfall: (1) Nyberg, J: Marin kartering längs Skånes kust. Projket skånestrand, katering av marina områden; (2) Borell, C: Erosion och kustskydd i Kävlinge kommun. Praktikfall; (3) Rydell, B: SGI 10 år som samordnare av stranderosion; (III) Tema 3: Planeringsunderlag: (1) Cooper, A: Coastal development in the British Isles. Contemporary approaches and issues in coastal development in the British Isles; (2) Askman, P: Multifunktionell kustplanering. Ett regionalt utvecklingsperspektiv; (3) Lindeberg, G: Användning av satellitbilder och Nationell höjdmodell; (4) Wikström, S: Laserskanning och flygbilder för kartering av kustzonen; (5) Alm, E: Vad kan vi tillåta med hänsyn till naturen? Tillståndsfrågor vid kustskydd; (6) Swenson, D: Coastal municipality master plan template; (7) Hanson, H: En exposé över skånska kustproblem; (IV) Senaste nytt: (1) Danielsson, P: På gång på SGI; (2) Nisser-Larsson, M: Nationella plattformen - naturolyckor; (3) Larson, M: På gång på LTH; (4) Sjöström, Å: Liten klimatuppdaterin
Exchange rate determination and inter–market order flow effects
No full textThe dependence of foreign exchange rates on order flow is investigated for four major exchange rate pairs, EUR/USD, EUR/GBP, GBP/USD and USD/JPY, across sampling frequencies ranging from 5 minutes to 1 week. Strong explanatory power is discovered for all sampling frequencies. We also uncover cross-market order flow effects e.g. GBP exchange rates are very strongly influenced by EUR/USD order flow. The Meese and Rogoff (1983a,b) framework is used to investigate the predictive power of order flow for exchange rate changes and it is shown that the order flow specifications reduce RMSEs relative to a random walk for all exchange rates at high-frequencies and for EUR/USD and USD/JPY at lower sampling frequencies
Typning av HLA-B*27: En jämförelsestudie mellan två analyser för att påvisa HLA-B*27 molekylen i Ankyloserande Spondylit
No full textTypning av hla-b*27:
En jämförelsestudie mellan två analysmetoder för att påvisa HLA-B*27 molekylen i ankyloserande spondylit
Carolina Bermudez
Bermudez, C. Typning av HLA-B*27. En jämförelsestudie mellan två analysmetoder för att påvisa HLA-B*27 molekylen i Ankyloserande spondylit. Examensarbete i Biomedicinsk vetenskap, 15 högskolepoäng. Malmö universitet: Fakulteten för hälsa och samhälle, institutionen för Biomedicinsk vetenskap, 2018.
Human leukocyt antigen (HLA) är vävnadsantigener, belägna på våra vita blodkroppar. HLA-B*27 allelen är starkt kopplat till Ankyloserande spondylit (AS). Det är en kronisk inflammatorisk ledsjukdom, som främst attackerar ryggraden, bäckenet och bröstkorgen. Det finns idag ingen enskild laborativ metod som med full säkerhet kan fastställa diagnos av denna sjukdom, innan de kliniska symtomen uppträder. Typning av HLA-B*27 ger endast information om närvaro eller frånvaro av antigenet, vid utredning av AS. Vidare är HLA-B*27 en polymorf och de olika alleltyperna varierar kraftigt, bland skilda etniska grupper samt mellan geografiska områden. Genetiska- och miljöfaktorer påverkar också. Sjukdomsutveckling i samband med närvaro av HLA-B*27 allelen, varierar därför från individ till individ. Därmed fungerar metoden endast som ett komplement-verktyg, för att ytterligare bekräfta diagnos. Syftet med denna studie var att med realtids-polymerase chain reaction (PCR), utföra typning av HLA-B*27 med Linkseq kit samt jämföra analysresultaten med uthämtade resultat från intern sjukhusdatabas, där typning av HLA-B*27 hade utförts med PCR-SSP (sekvens-specifika primers). Samtliga resultat stämde överens till 100%, vilket indikerar att metoden fungerar bra. Det finns studier som visat att HLA-B*27 molekylens fria tunga kedjor (HLA-B*272) har en starkare benägenhet än andra HLA-molekyler att binda in till killer immunoglobine-like receptorer (KIRs). Inbindning till KIRs med efterföljande ökad stimulering av interleukiner (IL) främst IL-17 och IL-23 bidrar till sjukdomsutvecklingen av AS. Dock finns ingen HLA-B*272 specifik antikropp som kan bevisa detta och det behövs därför ytterligare undersökning för att hitta en sådan. Därefter skulle en ny laborativ metod kunna utvecklas för att fastställa diagnos av AS i ett tidigt skede, innan de kliniska symtomen uppvisas.
Nyckelord: Allelvarianter, Ankyloserande spondylit, HLA-B*27, KIR, PCR-SSP, Realtids-PCR.typing of hla-b*27:
a comparison study between two analysing methods for the detection of the HLA-B*27 molecule in ankylosing spondylitis
Carolina Bermudez
Bermudez, C. Typing of HLA-B*27. A comparison study between two analysing methods for the detection of the HLA-B*27 molecule in Ankylosing spondylitis. Degree project in Biomedical Laboratory Science, 15 credit points. Malmö University: Faculty of Health and Society, Department of Biomedical science, 2018.
Human leukocyte antigen (HLA) are tissue antigens located on our white blood cells. The HLA-B*27 allele is strongly related to Ankylosing spondylitis (AS). It is a chronical inflammatory rheumatic disease that primarily affects the spine, the pelvis and the chest. At present, there is no single laboratory method that with all certainty may determine diagnosis of this disease, before the clinical symptoms appear. Typing of HLA-B*27 only gives information about the presence or absence of the antigen, upon the investigation of AS. Furthermore, HLA-B*27 is a polymorph and the different types of alleles, strongly vary among different ethnic groups and also between geographic regions. Genetic- and environmental factors also affect. Development of disease in conjunction with the presence of the HLA-B*27 allele, therefore varies from one individual to another. So, the method only functions as a complementary tool, to further confirm diagnosis. The aim of this study was to perform HLA-B*27 typing with realtime-polymerase chain reaction (PCR) using Linkseq kit and compare the analysed results with those results that were retrieved from the internal database of the hospital, where typing of HLA-B*27 had been performed with PCR-SSP (sequence specific primers). All results agreed with 100%, which indicates that the method functions well. There are studies that show that the heavy chains (HLA-B*272) of the HLA-B*27 molecule have a stronger affinity than other HLA-molecules of binding in to killer immunoglobulin-like receptors (KIRs). Increased stimulation of interleukins (IL) primarily IL17 and IL23, following binding to KIRs, contributes to the pathogenesis of ankylosing spondylitis. However, there is no HLA-B*272 specific antibody that may prove this and therefore more investigation is needed, in order to find one. A new laboratory method could then be developed to determine diagnosis of AS at an early stage, before the clinical symptoms emerge.
Keyword: Allelvariants, Ankylosing spondylitis, HLA-B*27, KIR, PCR-SSP, Realtime-PCR
Role of the hERG-channel in arrhythmia and teratogenicity : studies in animal models and the human embryonic heart
No full textBackground: Drugs that inhibit cardiac repolarization are associated with potentially life threatening side effects in the form of ventricular arrhythmias in humans. Animal studies show that this mechanism also is relevant for the embryo, and that the circulatory depression results in hypoxia with embryotoxicity in the form of malformations and death as a consequence. This thesis addresses the pharmacological arrhythmogenic effects on the rodent embryonic heart, and highlight the human relevance of this mechanism by characterization of the human embryonic heart.Methods and Results: Pregnant mice were administered the hypoxia probe pimonidazole followed by phenytoin or saline at gestation day (GD) 10 or 15. Phenytoin treatment resulted in dose-dependent embryonic staining for the hypoxia probe at GD 10. At GD 15 staining was not dose-dependent and less pronounced compared to controls.The effect on cardiac rate and rhythm of antiepileptic drugs (AEDs) was studied in cultured GD 10 mouse embryos. Phenytoin, dimethadione, carbamazepine and phenobarbital induced concentration-dependent prolongation of the inter-beat interval (IBI) and irregular arrhythmias. Exposure to combinations of AEDs in therapeutic concentrations resulted in significant increase in IBI compared to single exposure.ECG was obtained before and after drug exposure from GD 11 rat embryos and embryonic cardiomyocytes (ECMs) cultured in multi-electrode array (MEA) culture dishes. In the embryo model phenytoin and the selective IKr blocker E4031 both induced concentration-dependent bradyarrhythmia and QTC prolongation in cultured GD11 rat embryos. At the higher tested concentrations, phenytoin induced cardiac arrest and E4031 induced AV-nodal block. In the ECM model sensitivity to phenytoin and E4031 was similar but other arrhythmias were observed.The distribution of Isl1+ progenitor cells and their proliferative and differentiating capacity in human first trimester embryonic hearts were determined by immunohistochemistry. Isl1+ cells were present in the heart and a few were Ki67+ and troponinT+.Beating clusters of human embryonic cardiomyocytes, called cardiospheres were derived from human embryonic hearts and characterized with immunohistochemistry, electron microscopy and in the MEA system. The spheres were sensitive to adrenergic stimulation with isoprenaline and displayed rate dependency of the action potential in a pacing experiment. Expression and function of the two components of the delayed rectifier potassium current (IK), IKr and IKs, were characterized in cardiac tissue and ECMs from human, rat and rabbit embryonic hearts. Patch clamp and quantitative RT-PCR were used. IKr was expressed and functional in all species. IKs expression was found in human and rat but not in rabbit hearts.Conclusions: Phenytoin induces dose-dependent embryonic hypoxia. The studied AEDs induce concentration-dependent embryonic bradycardia and arrhythmia. For selective IKr blockers and phenytoin, the effects are associated with QT prolongation. This indicates that QT prolongation can be used as a biomarker for embryonic arrhythmogenicity. Rat ECMs display a similar sensitivity as the embryonic heart but respond differently to drug exposure. Isl1+ cells are present in the human embryonic heart and cardiospheres derived from embryonic hearts display rate dependency of the action potential duration and sensitivity to β-adrenergic stimulation. The IKr current is expressed and functional in the human embryonic heart and in species used in teratology testing. The results support that drug-induced embryonic arrhythmia is a cause of embryotoxicity and indicate human relevance of this mechanism.List of scientific papersI. Danielsson BR, Johansson A, Danielsson C, Azarbayjani F, Blomgren B, Sköld AC (2005). "Phenytoin teratogenicity: hypoxia marker and effects on embryonic heart rhythm suggest an hERG-related mechanism." Birth Defects Res A Clin Mol Teratol 73(3): 146-53 https://pubmed.ncbi.nlm.nih.gov/15744730II. Danielsson C, Azarbayjani F, Sköld AC, Sjögren N, Danielsson BR (2007). "Polytherapy with hERG-blocking antiepileptic drugs: increased risk for embryonic cardiac arrhythmia and teratogenicity." Birth Defects Res A Clin Mol Teratol 79(8): 595-603 https://pubmed.ncbi.nlm.nih.gov/17584909III. Genead R, Danielsson C, Wärdell E, Kjaeldgaard A, Westgren M, Sundström E, Franco-Cereceda A, Sylvén C, Grinnemo KH (2010). "Early first trimester human embryonic cardiac Islet-1 progenitor cells and cardiomyocytes: Immunohistochemical and electrophysiological characterization." Stem Cell Res 4(1): 69-76. Epub 2009 Nov 6 https://pubmed.ncbi.nlm.nih.gov/19896915IV. Danielsson C, Genead R, Andersson A, Sköld AC, Elsheikh E, Grinnemo KH, Hellmold H, Dencker L, Sylvén C (2010). "Drug-induced embryonic ar-rhythmia comparative in vitro electrophysiological studies in rat em-bryos and embryonic cardiomyocytes." (Manuscript)V. Danielsson C, Brask J, Andersson U, Stockling K, Klevenfeldt M, Wardell E, Genead R, Sköld AC, Grinnemo KH, Kjaeldsgaard A, Sundström E, Elinder F, Dencker L, Sylvén C (2010). "Electrophysiological development in the human, rat and rabbit embryo heart implications for drug-induced-arrhythmia related-teratogenicity." (Manuscript)</p
Adolescent alcohol use : implications for prevention
No full textBackground: Alcohol use, especially heavy episodic drinking, at an early age has been associated with various problems (e.g. risky sexual behaviours, health problems, depression, and heavy alcohol consumption at a later age). Thus, a better understanding of the risk and protective factors that influence adolescent alcohol use is crucial to developing effective prevention strategies. The aim of this thesis is to examine the importance of risk and protective factors in the development of heavy episodic drinking and subsequent problems for adolescent boys and girls. In addition, the prevention paradox (most alcohol-related problems occur in the 90 % of the population with lowest alcohol consumption) was examined among adolescents in Sweden and Europe.Methods: Data from three different questionnaire studies were analysed: (1) a longitudinal cohort study with 1222 adolescents from Stockholm, aged 13 to 19 years, (2) a cross-sectional study with 3000 adolescents aged 15 years and 17 years from random samples of school classes throughout the whole of Sweden, and (3) a cross-sectional study (the European School Survey Project on Alcohol and Other Drugs, ESPAD) performed in 35 countries among students who turned 16 during the year of the data collection. Twenty-three countries with 38 370 alcohol-consuming adolescents were included.Results: Smoking and peer alcohol use were strongly associated with heavy drinking among both boys and girls, both cross-sectionally and longitudinally. Some gender differences were found; parental provision of alcohol in the 7th grade increased the odds for heavy alcohol use in girls two years later, and truancy was associated with later heavy alcohol use in boys. For boys, heavy episodic drinking at age 13 was one of the most distinct predictors of later heavy episodic drinking. For girls, secure bonds to parents lowered the risk for heavy episodic drinking, even if the girls had friends who drank alcohol, money to spend, or parents who offered them alcohol. For boys whose parents offered them alcohol, parental monitoring had a protective effect. Also, we found that adolescents on a consistent high alcohol use trajectory during early adolescence had higher levels of heavy episodic drinking and alcohol-related problems at age 19. Furthermore, the prevention paradox was valid for adolescent boys and girls in Sweden and in most European countries; despite differences in annual alcohol consumption, levels of heavy episodic drinking, and reported problems, the heavy episodic drinkers in the bottom 90% consumer group accounted for a majority of all reported problems.Conclusions: Effective population strategies may have large potential to reduce risk drinking and the overall problem level. A comprehensive prevention strategy should nevertheless also include efforts to reach adolescent high consumers. Furthermore, our results lend support to prevention initiatives to strengthen the parent–child relationship, to focus on adolescents‟ ability to resist peer pressure, and to limit parental provision of alcohol.List of scientific papersI. Danielsson, AK., Romelsjö, A. & Tengström. A. Heavy episodic drinking in early adolescence: Gender-specific risk and protective factors. Substance use & misuse. [Accepted] https://doi.org/10.3109/10826084.2010.528120 II. Danielsson, AK., Wennberg, P., Tengström. A. & Romelsjö, A. (2010). Adolescent alcohol use trajectories: Predictors and subsequent problems. Addictive Behaviors. 35, 848-852. https://doi.org/10.1016/j.addbeh.2010.05.001III. Romelsjö, A. & Danielsson, AK. Does the prevention paradox apply to various alcohol habits and problems among adolescents? [Submitted] https://doi.org/10.1093/eurpub/ckr178IV. Danielsson, AK., Wennberg, P., Hibell, B. & Romelsjö, A. Alcohol use, heavy episodic drinking, and subsequent problems among adolescents in 23 European countries: does the prevention paradox apply? [Submitted] https://doi.org/10.1111/j.1360-0443.2011.03537.x</p
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