1,720,962 research outputs found
IN SILICO IDENTIFICATION OF TARGET PALINDROMIC GENES AS POTENTIAL DRUG TARGETS IN BREAST CANCER THERAPY
Get PDFObjectives: Breast cancer (BC) is the most common cancer worldwide prevalent among women with more than one million cases and is second only to lung cancer.
Methods: The identification of the sequences based on the unique tetramers GCAC, GTCA were selected from experimental work. The16 base pair DNA regulatory sequences of which the motifs area part of containing these motif in genes implicated in cancer CAGE1 (AAGCTGTCATTA), BRCA1(GACTGAGTCAA), ABCB1(CTCTAAGTCAT), ABCB5 (GATATGTTAAAGC) and ABI1(CTTCTGGGAA) were then selected as novel putative targets in breast cancer therapy based on their selectivity on the BC oncogenes which are not found in the normal human genome 1-23 and the sex chromosomes X and Y were obtained via computational analysis.
Results: The single copy base pairs which will be potential drug targets as anticancer drugs were finally obtained as CTGTTATGACTGAGTCAA, CAGE1 with the 17 base pairs CATAAAAGC TGTCATTA and ABCB1 TTGCCAA CTCTAAGT CAT.
Conclusion: It is Possible that the in silico discovery of putative anti breast cancer targets of importance in the genome.
Peer Review History:
Received 7 August 2020; Revised 13 September; Accepted 28 October, Available online 15 November 2020
Academic Editor: Prof. Kumud Upadhyaya, Kumaon University, Nainital, UK, India, [email protected]
Reviewer(s) detail:
Dr. Nkechi Obiofu Ezenobi, University of Port Harcourt, Nigeria, [email protected]
Shahin Gavanji, World Academy of Medical Sciences, Iran, [email protected]
IN SILICO ANTIMALARIAL TARGET SELECTION CONSERVED IN FOUR PLASMODIUM SPECIES
Get PDFObjectives: The need for new antimalarials drugs and drug targets is pertinent due to the emergence of drug resistant strains of the parasites. Improper target selection has resulted in therapeutic failure. The genomic/post genomic era has made possible the deciphering of the 3D crystal structures of proteins and DNA which are drug targets and are deposited in the protein data bank.
Methods: Novel antimalarial targets obtained from evolutionary conserved short sequence motifs are utilised and are essential in transcription processes in the parasite. The motifs TGCATGCA, GTGCAC and GTGCGTGC were curated from experimental work, validated and analysed via phylogenomics genomics and comparative genomics. PlasmoDB blastn was applied to determine their similarity in Plasmodium vivax, knowlesi, Ovale and yoeli. The complete genome of Plasmodium falciparum vivax, knowlesi, Ovale and yoeli was downloaded from the plasmoDB and their positions determined.
Results: The targets are essential, conserved in rodent and mammalian species via phylogenomics with percentage identity and similarity greater than 80%, have no similar genes in the same genome and also found to be selective in the parasites vis-à-vis the Homo sapiens via comparative genomics with 0% identity and similarity in the human genome.
Conclusion: The targets reveal at the molecular and biochemical level, the vulnerable regions in the parasite while safe in human hence their choices in subsequent rationale drug discovery and design protocols.
Peer Review History:
Received: 8 August 2020; Revised: 11 September; Accepted: 23 October; Available online: 15 November 2020
Academic Editor: Dr. Sally A. El-Zahaby, Pharos University in Alexandria, Egypt, [email protected]
Reviewer(s) detail:
Dr. Tamer ELHABIBI, ERU University, Egypt, [email protected]
Dr. Soroush Sardari, Biotech Pasteur Institute of Iran, Tehran, Iran, [email protected]
The Application of Whole Genome Sequencing in Medical and Pharmaceutical field: -
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Whole Genome Sequencing (WGS) is a high throughput process utilized to obtain the nucleotide bases that constitutes a genome. The Sanger Technique was used to sequence the first human genome; however the limitations include inability to sequence more than few genes per time and it cannot detect structural rearrangement. The method also has far reaching cost implications. The New Generation Sequencing Platform has further lend credence to WGS, as sequencing can be carried out in a shorter period of time with accuracy hence a great improvement in the clinical setting for diagnostic purposes. This also needs to correspond with effective data analysis and interpretation. This method has proved useful as it can be used to compare bacterial strains that have a divergence at one nucleotide base, transmission, Virulence, phenotypic characteristics can be deduced. Various strains can be analyzed via a single process and it is an essential tool in clinical diagnosis and in monitoring the outbreak of a new disease. This is important in handling multidrug resistance strains of bacteria. In addition, it equally helps in the identification of drug response markers on drug target. Moreover, drug metabolism or disease pathway can enhance development of drug tailored to specific individuals for specific purposes, hence the ability to define a population genetically
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Establishment of criminal/profile DNA database and use of forensic intelligence to combat nationwide insecurity issues in Nigeria
Get PDFCriminal activity is on the rise in Nigeria. The nefarious activity of bandits is on the increase with kidnapping of students and attack on farmers. This creates fear in the terrorized communities. Armed robbery as well as rape of citizens going about their normal duties is also on the increase. The road is becoming increasingly unsafe for the citizenry as well as government officials. The government has mandated individuals to register in the National Identification Number NIN which is linked to the Bank Verification Number (BVN) (a database established to curb crime in the banking sector) and the Subscriber Identity module (SIM). The Establishment of such databases is pertinent however the NIN, BVN, SIM-NIN linkage and fingerprints may not be sufficient in solving criminal cases without DNA profiling of convicted individuals, establishment of forensic laboratories and the creation of criminal DNA profile database CRODER – a Criminal offender DNA Exert Repertoire. The role of DNA profiling as an additional measure is to keep a record of all convicted individuals and the CRODER will be an additional useful measure in profiling criminals and individuals can be adopted as a forensic tool in addition to the captured databases of NIN, BVN and NIN-SIM and will be on the watch list of security operatives. The CRODER forensic tool can be done stepwise by availability of an emergency number to dial for emergency, a dispatch personnel to respond to the call, and vigilante groups or other adhoc security officer in the vicinity of the emergency call to get to the vicinity and preserve the crime scene environment for investigators and detectives to work on. CRODER Forensic tool can then be adopted to solve the crime. Offenders trying to escape enroute the airport, can be checked via CRODER and fingerprint compared and matched with the collection in the database. The CRODER may be a necessary stride to bring kidnappers, bandits, and armed robbers to book in record time and curb the rise in criminal activity, insurgency, banditry, and insecurity in general. If properly done, Nigeria will be a haven for citizens and would-be investors
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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