61 research outputs found
Evaluation of the expression of matrix metalloproteinase 9 and neutrophil gelatinase associated lipocalin in serum, urine and tumoral tissues of female dogs suffering from mammary gland tumors
No full textIn human medicine, it has been shown recently that the level of expression of matrix metalloproteinase-9 (MMP-9) and neutrophil gelatinase-associated lipocalin (NGAL) in serum, urine, and breast tissue were significantly increased in patients with breast cancer and correlated to several prognostic factors. In the first part, the author presents the MMP-9 and NGAL as a synthesis of current knowledge on their ability to serve as biomarkers of breast cancer in women. In the second, the author presents the production of canine MMP9 and NGAL recombinant proteins, the production of polyclonal antibodies, and their use in various techniques (ELISA, western blot, immunohistochemistry) to assess the level of expression of these proteins in the blood, urine and breast tissue of dogs suffering from mammary gland tumors and demonstrate a positive correlation between these proteins and the presence of a tumor disease of the mammary gland
Total and partial cancer prevalence in the adult French population in 2008.
No full textBACKGROUND: To provide estimations of partial and total prevalence of 24 cancer sites in France in 2008. The estimations of partial prevalence were compared with the previous estimations for 2002. METHODS: Nationwide estimations of incidence and survival data from cancer registries were used for partial prevalence. Nationwide incidence and mortality data were used to estimate total prevalence. RESULTS: At the end of 2008, in France, nearly 3 million people still alive had received a diagnosis of cancer. Of all prevalent cases, 36% were diagnosed 0 to 5 years earlier and 43% diagnosed 6 to 10 years earlier. The cancer sites with the highest prevalence were the prostate, the breast, and the colon-rectum. The changes in partial prevalence over 5 years (2002 to 2008) were considerable (+244,000 cases) and deemed to be highly related to changes in incidence. CONCLUSION: The present estimations update the French prevalence data and highlight the burden of cancer in the population, especially in the elderly. The methods of this study had the advantage of using recent incidence and survival data, which is necessary to show sudden changes in incidence trends and changes in survival that impact prevalence
Hazard regression model and cure rate model in colon cancer relative survival trends: are they telling the same story?
No full textHazard regression models and cure rate models can be advantageously used in cancer relative survival analysis. We explored the advantages and limits of these two models in colon cancer and focused on the prognostic impact of the year of diagnosis on survival according to the TNM stage at diagnosis. The analysis concerned 9,998 patients from three French registries. In the hazard regression model, the baseline excess death hazard and the time-dependent effects of covariates were modelled using regression splines. The cure rate model estimated the proportion of 'cured' patients and the excess death hazard in 'non-cured' patients. The effects of year of diagnosis on these parameters were estimated for each TNM cancer stage. With the hazard regression model, the excess death hazard decreased significantly with more recent years of diagnoses (hazard ratio, HR 0.97 in stage III and 0.98 in stage IV, P 0.5). The two models were complementary and concordant in estimating colon cancer survival and the effects of covariates. They provided two different points of view of the same phenomenon: recent years of diagnosis had a favourable effect on survival, but not on cure
Incidence of HIV-related anal cancer remains increased despite long-term combined antiretroviral treatment: results from the french hospital database on HIV.
No full textPURPOSE: To study recent trends in the incidence of anal cancer in HIV-infected patients receiving long-term combined antiretroviral treatment (cART) compared with the general population. PATIENTS AND METHODS: From the French Hospital Database on HIV, we identified 263 cases of invasive anal squamous cell carcinoma confirmed histologically between 1992 and 2008. We compared incidence rates of anal cancer across four calendar periods: 1992-1996 (pre-cART period), 1997-2000 (early cART period), and 2001-2004 and 2005-2008 (recent cART periods). Standardized incidence ratios (SIRs) were calculated by using general population incidence data from the French Network of Cancer Registries. RESULTS: In HIV-infected patients, the hazard ratio (HR) in the cART periods versus the pre-cART period was 2.5 (95% CI, 1.28 to 4.98). No difference was observed across the cART calendar periods (HR, 0.9; 95% CI, 0.6 to 1.3). In 2005-2008, HIV-infected patients compared with the general population had an excess risk of anal cancer, with SIRs of 109.8 (95% CI, 84.6 to 140.3), 49.2 (95% CI, 33.2 to 70.3), and 13.1 (95% CI, 6.8 to 22.8) for men who have sex with men (MSM), other men, and women, respectively. Among patients with CD4 cell counts above 500/μL for at least 2 years, SIRs were 67.5 (95% CI, 41.2 to 104.3) when the CD4 nadir was less than 200/μL for more than 2 years and 24.5 (95% CI, 17.1 to 34.1) when the CD4 nadir was more than 200/μL. CONCLUSION: Relative to that in the general population, the risk of anal cancer in HIV-infected patients is still extremely high, even in patients with high current CD4 cell counts. cART appears to have no preventive effect on anal cancer, particularly in MSM
Evaluation de l'expression de la matrix métalloprotéinase 9 et de la neutrophil gelatinase associated lipocalin dans le sérum, l'urine et le tissu tumoral de chiennes atteintes de tumeurs mammaires
No full textIn human medicine, it has been shown recently that the level of expression of matrix metalloproteinase-9 (MMP-9) and neutrophil gelatinase-associated lipocalin (NGAL) in serum, urine, and breast tissue were significantly increased in patients with breast cancer and correlated to several prognostic factors. In the first part, the author presents the MMP-9 and NGAL as a synthesis of current knowledge on their ability to serve as biomarkers of breast cancer in women. In the second, the author presents the production of canine MMP9 and NGAL recombinant proteins, the production of polyclonal antibodies, and their use in various techniques (ELISA, western blot, immunohistochemistry) to assess the level of expression of these proteins in the blood, urine and breast tissue of dogs suffering from mammary gland tumors and demonstrate a positive correlation between these proteins and the presence of a tumor disease of the mammary gland.TOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF
Évaluation de l'expression de la matrix métalloprotéinase 9 et de la neutrophil gelatinase associated lipocalin dans le sérum, l'urine et le tissu tumoral de chiennes atteintes de tumeurs mammaires
No full textIn human medicine, it has been shown recently that the level of expression of matrix metalloproteinase-9 (MMP-9) and neutrophil gelatinase-associated lipocalin (NGAL) in serum, urine, and breast tissue were significantly increased in patients with breast cancer and correlated to several prognostic factors. In the first part, the author presents the MMP-9 and NGAL as a synthesis of current knowledge on their ability to serve as biomarkers of breast cancer in women. In the second, the author presents the production of canine MMP9 and NGAL recombinant proteins, the production of polyclonal antibodies, and their use in various techniques (ELISA, western blot, immunohistochemistry) to assess the level of expression of these proteins in the blood, urine and breast tissue of dogs suffering from mammary gland tumors and demonstrate a positive correlation between these proteins and the presence of a tumor disease of the mammary gland.En médecine humaine, il a été montré récemment que le niveau d'expression de la matrix métalloprotéinase-9 (MMP-9) et de la neutrophil gelatinase-associated lipocalin (NGAL) dans le sérum, l'urine, et le tissu mammaire étaient significativement augmentés chez les patientes atteintes de cancer du sein et étaient corrélés à de nombreux facteurs pronostiques. Dans une première partie, l'auteur présente la MMP-9 et la NGAL ainsi qu'une synthèse des connaissances actuelles portant sur leurs aptitudes à servir de biomarqueurs du cancer du sein chez la femme. Dans la seconde, l'auteur présente la production de MMP9 et de NGAL canines recombinantes, la production d'anticorps polyclonaux associés, et leur utilisation dans diverses techniques (ELISA, western blot, immunohistochimie) permettant d'évaluer le niveau d'expression de ces protéines dans le sang, l'urine et le tissu mammaire de chiennes malades et de démontrer ainsi une corrélation positive entre ces protéines et la présence d'une affection tumorale de la glande mammaire
Évaluation de l'expression de la matrix métalloprotéinase 9 et de la neutrophil gelatinase associated lipocalin dans le sérum, l'urine et le tissu tumoral de chiennes atteintes de tumeurs mammaires
No full textIn human medicine, it has been shown recently that the level of expression of matrix metalloproteinase-9 (MMP-9) and neutrophil gelatinase-associated lipocalin (NGAL) in serum, urine, and breast tissue were significantly increased in patients with breast cancer and correlated to several prognostic factors. In the first part, the author presents the MMP-9 and NGAL as a synthesis of current knowledge on their ability to serve as biomarkers of breast cancer in women. In the second, the author presents the production of canine MMP9 and NGAL recombinant proteins, the production of polyclonal antibodies, and their use in various techniques (ELISA, western blot, immunohistochemistry) to assess the level of expression of these proteins in the blood, urine and breast tissue of dogs suffering from mammary gland tumors and demonstrate a positive correlation between these proteins and the presence of a tumor disease of the mammary gland.En médecine humaine, il a été montré récemment que le niveau d'expression de la matrix métalloprotéinase-9 (MMP-9) et de la neutrophil gelatinase-associated lipocalin (NGAL) dans le sérum, l'urine, et le tissu mammaire étaient significativement augmentés chez les patientes atteintes de cancer du sein et étaient corrélés à de nombreux facteurs pronostiques. Dans une première partie, l'auteur présente la MMP-9 et la NGAL ainsi qu'une synthèse des connaissances actuelles portant sur leurs aptitudes à servir de biomarqueurs du cancer du sein chez la femme. Dans la seconde, l'auteur présente la production de MMP9 et de NGAL canines recombinantes, la production d'anticorps polyclonaux associés, et leur utilisation dans diverses techniques (ELISA, western blot, immunohistochimie) permettant d'évaluer le niveau d'expression de ces protéines dans le sang, l'urine et le tissu mammaire de chiennes malades et de démontrer ainsi une corrélation positive entre ces protéines et la présence d'une affection tumorale de la glande mammaire
Direct modeling of the crude probability of cancer death and the number of life years lost due to cancer without the need of cause of death: a pseudo-observation approach in the relative survival setting.
No full textIn population-based cancer studies, net survival is a crucial measure for population comparison purposes. However, alternative measures, namely the crude probability of death (CPr) and the number of life years lost (LYL) due to death according to different causes, are useful as complementary measures for reflecting different dimensions in terms of prognosis, treatment choice, or development of a control strategy. When the cause of death (COD) information is available, both measures can be estimated in competing risks setting using either cause-specific or subdistribution hazard regression models or with the pseudo-observation approach through direct modeling. We extended the pseudo-observation approach in order to model the CPr and the LYL due to different causes when information on COD is unavailable or unreliable (i.e., in relative survival setting). In a simulation study, we assessed the performance of the proposed approach in estimating regression parameters and examined models with different link functions that can provide an easier interpretation of the parameters. We showed that the pseudo-observation approach performs well for both measures and we illustrated their use on cervical cancer data from the England population-based cancer registry. A tutorial showing how to implement the method in R software is also provided
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