14 research outputs found
The Effect of Green Tea Extract Supplementation on Plasma Malondialdehyde (MDA) in Human with Sub-Maximal Physical Activity, Double-Blind and Placebo-Controlled Trials
No full textIntroduction. Green Tea extract has been proven in vitro to have the ability as an antioxidant. Submaximal physical activity causes an increase in free radicals. The increase of MDA can detect the increase of free radicals. This study aimed to determine the effects of green tea extract supplementation against free radicals in submaximal physical activity by measuring plasma MDA.
Methods. This double-blind controlled trial study was performed in 20 students of faculty of sports science with submaximal physical activity in the form of 1,500 meters running in 12 minutes using a treadmill. Subjects were divided into two groups: green tea extract group (Tegreen 250 mg) and the control group (placebo). Supplement/placebo was given as one caps a day for fourteen days before submaximal physical activity. The plasma MDA was taken before, 2 hours after, and 48 hours after the submaximal physical activity. Analysis of the results used ANOVA and post hoc Tukey. The study has been assessed by Medical Research Ethical Committee at Medical Faculty of Universitas Brawijaya.
Results. In the control group, the mean value of plasma MDA levels in basal conditions, 2 hours and 48 hours after submaximal physical activity were 52.43 nmol/ml (standard deviation [SD] 12.52 nmol/ml), 55.57 nmol/ml (SD 13.84 nmol/ml), and 63.86 nmol/ml (SD 12.17 nmol/ml), respectively. However, this result was not statistically significant (p = 0.158). In the treatment group, the mean value of plasma MDA level at 48 hours after submaximal physical activity was significantly decreased compared to basal condition with mean value in basal and 48 hours after submaximal physical activity were 36.14 nmol/ml (SD 5.88 nmol/ml) and 19.86 nmol/ml (SD 8.92 nmol/ml), respectively (p <0.001). Further analysis showed a significant difference in plasma MDA levels between the two groups at basal, 2 hours, and 48 hours after submaximal physical activity (p values were 0.012; <0.001, and <0.001 respectively).
Conclusion. Green tea extract can decrease the plasma level of MDA at basal, 2 hours, and 48 hours after submaximal physical activity
Correlation of Ramadan Fasting with HbA1C and Lipid Profile Level Changes in The Risk Stratification of Complications in Diabetes Mellitus Patients
No full textIntroduction. Diabetic patients will still do Ramadan fasting, which could affect the levels of HbA1C and lipid change after Ramadan. This study aimed to identify the correlation between fasting of Ramadan risk stratification with HbA1C and lipid levels diabetic patients who run the fast of Ramadan.
Methods. This was a survey research using pre - post test. Study was conducted among diabetes patients who meet the criteria: carry out routine controls before and after Ramadan fasting at dr. Saiful Anwar Malang Hospital, filling in the required data, and underwent examination laboratory. Patients were grouped into moderate, high, and very high-risk stratification. Then we analyzed the HbA1C and fat levels before (pre) and after (post) Ramadan fasting in each risk group.
Results. There were 25 subjects included in this study. There was a decrease in HbA1C level in all subjects from 7.93% (SD 2.3) to 7.86% (SD 1.9) after Ramadan fasting (p = 0.563), only the moderate risk group increased by 0,37% (SB 0.27). In all subjects, total cholesterol levels increased from 196.4 (SD 48.7) mg/dl to 205.0 (SB 70.8) mg/dl (p = 0.397), with the highest change found in the very high-risk group with an increase 30,7 (SD 125.0) mg/dl. There was a decrease in HDL levels from 55.6 (SD 31.3) mg/dl to 47.8 (SD 10.5) mg/dl (p = 0.782), and the highest decrease occurred in the very high - risk group (27.7 (SD 66,9) mg / dl). There was an increase in the LDL from 115.9 (SD 45.8) mg/dl to 130.4 (SD 41.9) mg/dl (p = 0.133) which the highest increase found in the very high-risk group (55.0 (SD 74.7) mg/dl). Only triglycerides showed an improvement from 190.7 (SD 105.2) mg/dl to 188.8 (SD 79.6) mg/dl (p = 0.084), increasement only occur in the high risk group (13.2 (SD 213.4) mg/dl).
Conclusion. There was a decrease in HbA1C levels, but the value of the statistics was not significant. There was a negative effect of fasting Ramadan against blood-fat levels, except for improvements in the levels of triglycerides though statistically not significant
The Effect of Soya and Genistein Supplementation on Serum Lipid Profile of Male Dawley Sprague Rat
No full textBackground: Non-medical therapy is needed to reduce blood lipid levels and prevent atherosclerosis. Foods that contain Isoflavones can improve lipid profiles in the blood.Aim: To determine the effect of soya and genistein supplementation in various doses on total cholesterol, Low Density Lipoprotein (LDL), High Density Lipoprotein (HDL), Triglycerides in male Sprague-Dawley rats.Methods: 35 male Sprague-Dawley rats aged 6-8 weeks were divided into 7 groups, control group; low, medium, and high doses of soy milk; and low, moderate, and high doses of genistein. The treatment was given orally for 60 days. The control used was mice with standard feed. On the 61st day, blood and hepatic tissue samples were taken and checked for lipid profiles using the Friedewald formula. Statistical analysis was carried out by one-way ANOVA and Tukey\u27s posthoc test and Dunnet test, then regression analysis.Results: There was a significant increase in total cholesterol levels (p <0.05) and  LDL levels in low and moderate doses of soy milk group compared to all groups of genistein. There was no significant differences in HDL levels in soya and genistein groups. There was significant increase in Triglyceride levels in all groups of soy milk and low and moderate doses of Genistein compared to high doses of genistein.Conclusion:  Soya and genistein supplementation had increased effect on the Total Cholesterol, LDL and Triglycerides but no effect on HDL in all groups
Comparison of Lipid Profiles in Patients with Type 2 Diabetes Mellitus with Good Glycemic Control and Poor Glycemic Control in RSUD Dr. Saiful Anwar Malang
No full textIntroduction. Diabetes mellitus (DM) is a group of metabolic diseases characterized by hyperglycemia due to insulin resistance, deficiency of insulin action, or both with a prevalence of 8.5% in Indonesia. Evidence regarding the relationship between HbA1c and lipid profile in type 2 DM is currently contradictory, therefore a study was conducted to examine the relationship between glycemic control and lipid profile for DM patients.
Methods. This survey research was conducted at the outpatient endocrine clinic Dr. Saiful Anwar Malang Hospital in 2014-2019 with a total of 1,308 patients. Patients were then classified into type 2 DM patients with good (HbA1c 7%, n = 1017).
Results. There were lower levels of total cholesterol (183.9 (SD 38.7) mg/dl vs. 198.6 (SD 44.8) mg/dl; p < 0.001), triglycerides (144.2 (SD 67.5) mg/dl vs. 172.9 (SD 112) mg/dl; p < 0.001), and low-density lipoprotein (LDL-C) (119.2 (SD 32.1) mg/dl vs. 131 (SD 35.4) mg/dl; p < 0.001) in type 2 DM patients with good glycemic control compared to poor glycemic control group. There was no significant difference in high-density lipoprotein (HDL-C) levels between the two groups (47.7 (SD 13.4) mg/dl vs. 47.5 (SD 12.7) mg/dl; p = 0.89). There was a significant correlation between total cholesterol levels (r = 0.232; p < 0.001), triglycerides (r = 0.223; p < 0.001), and LDL-C (r = 0.20; p < 0.001) with HbA1c levels.
Conclusion. Type 2 DM patients with good glycemic control had significantly lower total cholesterol, triglycerides, and LDL-C levels compared to type 2 DM patients with poor glycemic control
The Effect of Diabetes Melitus and Blood Glucose on Patients Mortality with Severe COVID-19 Pneumonia Receiving Corticosteroids in RSUD Dr. Saiful Anwar Malang
No full textBackground: Coronavirus Disease 2019 (COVID-19) is an infectious disease caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). WHO has recommended the use of systemic corticosteroid therapy in patients with severe and critical COVID-19 pneumonia. However, the use of systemic corticosteroids is also a major contributor to hyperglycemia which is a strong prognostic predictor of poor outcome in hospitalized patients with COVID-19. Furthermore, diabetes mellitus (DM) is one of the most widely reported comorbidities in COVID-19 patients which affects the risk of morbidity and mortality and systemic corticosteroids can aggravate hyperglycemia in patients with this comorbidity. Objective: This study aims to determine the effect of DM and blood glucose on mortality in patients with severe COVID-19 pneumonia who received corticosteroids. Methods: The design of this study was an observational retrospective which was conducted on the medical records of COVID-19 patients who received convalescent plasma therapy at RSUD Dr. Saiful Anwar Malang (RSSA). Of the 229 patients who received convalescent plasma therapy and had fairly complete data, there were 85 patients with severe COVID-19 pneumonia who received corticosteroids with blood glucose data for analysis. Results: There were significant differences in mortality rates in DM and non-DM patients (OR 3.091; CI 1.232-7.756; p=0.015) with severe COVID-19 pneumonia who received corticosteroids. Analysis of blood glucose levels shows that the mean random blood glucose (RBG) and initial RBG levels were higher in patients with a death outcome compared to those who lived (mean RBG 176.07 ± 72.0 mg/dl VS 133.23 ± 56.38 mg /dl, p=0.02; initial RBG 198.40 ± 127.13 mg/dl VS 141.57 ± 73.28 mg/dl, p=0.022. Conclusion: DM and blood glucose levels (mean RBG and initial RBG) affect mortality rates in patients with severe COVID-19 pneumonia who are given corticosteroids. Keywords: COVID-19, diabetes mellitus, blood glucose, corticosteroids DOI: 10.7176/JHMN/102-07 Publication date:October 31st 202
Correlation between Risk Stratification of Complications and Types of Antihyperglycemia Drugs with Incidence of Acute Diabetic Complications in Patients with Diabetes Mellitus during Ramadan Fasting
No full textRamadan fasting for Diabetes Mellitus (DM) patients can lead to acute complications such as hypoglycemia, hyperglycemia, diabetic ketoacidosis (DKA) and thrombosis. Risk stratification predicts fasting safety of DM patients. Dose and timing of antihyperglycemia drugs adjusted during Ramadan fasting. Aim: To know the correlation between the risk stratification of Ramadan fasting and type of antihyperglycemia drugs with the incidence of acute complications in DM patients undergoing Ramadan fasting. Methods: DM patients in Endocrinology Clinic, dr. Saiful Anwar, General Hospital Malang who intend to fast during Ramadan classified in IDF-DAR risk stratification, conduct blood glucose monitoring and filled out a daily logbook during fasting. Results: Thirty-seven subjects were included in the study, only 1 patient with type-1 DM. Average fasting time is 18 days. Acute complications found higher in very high-risk group (5/6) compared to mild/moderate (2/13) and high-risk group (7/18) (p=0.009). Acute complications found higher in group with OAD and insulin combination regiment (2/4) compared to OAD (9/24) or insulin group (3/9) (p= 0. 731). One subject in very high-risk group suffered from acute coronary syndrome. Relationship between risk stratification and the incidence of hypoglycemia (p=0.040) and hyperglycemia (p=0.031) was significant. Relative risk in the very high-risk group was 2.538 compared to mild/moderate risk RR (95% CI)= 0.77 (0.62-0.96). Conclusions: There is a correlations between risk stratification and acute complications in DM patients undergoing Ramadan fasting. No relationship between type of antihyperglycemia drugs with acute complications of Ramadan fasting
Expression of Testicular Estrogen Receptor Alpha in Rats Exposed to Subchronic Inhalation Exposure of Transfluthrin
No full textEndocrine Disrupting compounds (EDCs) are exogenous materials that can interfere with the functions of the endocrine system. Research has demonstrated that pyrethroid compounds affect endocrine function. Transfluthrin belongs to the pyrethroid group that is widely used. These compounds could interact with estrogen receptor α and may increase the expression of estrogen receptor α in rat testis. Aim: to determine the effect of subchronic inhaled transfluthrin exposure on the expression of estrogen receptor α (ERα). Method: we used 35 adult male Wistar rats as experimental animals, which were randomly divided into 5 groups (n=7 per group): negative control group (rats without treatment), solvent control group (rats exposed to n-hexane solvent), and three treatment groups of subchronic inhaled transfluthrin: 0.1 mg/ml (treatment 1), 0.2 mg/ml (treatment 2) and 0.4 mg/ml (treatment 3), treated for 60 days. The immunohistochemical (IHC) staining was used to assess the expression of ERα. Result: there was a tendency for an increase in ERα expression with the addition of a transfluthrin dose. ER α in negative control group, solvent control group, treatment 1 group, treatment 2 group, and treatment 3 group was 16.75 ± 5.01 %; 16.64 ± 13.00 %; 19.31 ± 6.52 %; 25.63 ± 7.08 %, and 28.79 ± 20.31 %; p>0.05, respectively. There was a weak positive correlation between the dose of transfluthrin with ERα expression (r = 0.38, p = 0.025). Conclusion: subchronic inhalation exposure of transfluthrin in rats showed a tendency to increased the expression of ERα (dose-dependent). There is a weak positive correlation between the dose of transfluthrin with ERα expression.                                              Â
Pengaruh Kontrol Profil Lipid Terhadap Profil Hemostasis Dengan Menggunakan Metode Tromboelastografi Pada Pasien Diabetes Melitus Tipe 2
No full textPendahuluan: Resistensi insulin pada Diabetes Mellitus umumnya dikaitkan
dengan patogenesis dislipidemia. Hiperglikemia dan dislipidemia diketahui
sebagai faktor risiko aterosklerosis dan meningkatkan risiko trombosis.
Tromboelastografi (TEG) sebagai salah satu metode penilaian hemostasis, belum
rutin diterapkan pada pasien DM tipe 2 (Diabetes Mellitus). Oleh karena itu,
penelitian ini bertujuan untuk mengetahui pengaruh kontrol profil lipid pada pasien
DM tipe 2 terhadap profil hemostasis menggunakan TEG.
Metode: Penelitian ini adalah survey dengan analisis case control. Subyek
penelitian adalah pasien DM tipe 2 di poliklinik Endokrin RSUD dr. Saiful Anwar
yang memenuhi kriteria inklusi. Subyek terbagi dalam 2 kelompok yakni kelompok
profil lipid terkontrol dan tidak terkontrol sesuai nilai kolesterol total, HDL (High
Density Lipoprotein), LDL (Low Density Lipoprotein), dan TG (Trigliserida). Profil
hemostasis dianalisis menggunakan TEG dan terbagi dalam parameter
hemostasis yaitu nilai MA, nilai R & sudut-, dan fibrinolisis yaitu LY30. Uji statistik
dilakukan dengan analisis korelasi dan regresi logistik menggunakan software
SPSS 24.0. Nilai p signifikan bila <0.05.
Hasil: Terdapat 57 subyek yang memenuhi kriteria inklusi. Sebagian besar subyek
berada dalam kondisi normokoagulabel. Uji regresi logistik menunjukkan
perbedaan bermakna pada hemostasis sekunder (nilai R) terhadap kontrol HDL (p
< 0,05). Nilai R lebih tinggi pada kelompok pasien DM dengan kadar HDL tidak
terkontrol. Analisis korelasi HDL pada nilai R menunjukkan korelasi negatif yang
signifikan (p = 0,015) Tidak ada perbedaan yang signifikan pada kelompok pasien
dengan kadar kolesterol total, trigliserida dan LDL tidak terkontrol atau terkontrol
pada fibrinolisis primer, sekunder, dan tersier sebagai diukur dengan TEG.
Kesimpulan: Kontrol profil lipid (kolesterol total, TG, HDL dan LDL) berpengaruh
pada hemostasis (nilai R & sudut-). Kadar HDL berpengaruh signifikan terhadap
hemostasis (nilai R) dengan korelasi negatif. Penelitian ini merupakan uji coba
pertama untuk melihat fisiologi hemostasis menggunakan metode TEG dalam
kaitannya dengan kondisi dislipidemia pada pasien DM tipe
