119,866 research outputs found
Learning from transgenics: Advanced gene editing technologies should also bridge the gap with traditional genetic selection
No full textWe highlight the importance of the mixed genetic approaches (classical and currents) to improve the social perception related to the GMOs acceptance. We pointed out that CRISPR/Cas9 events could carry DNA variability/rearrangements related to somaclonal variations or epigenetic changes that are independent from the editing per se. The transformation of single cells, followed by plant regeneration, is used to generate modified plants, transgenic or genome editing (CRISPR/Cas9). The incidence of undesirable somaclonal variations and/or epigenetic changes that might have occurred during in vitro multiplication and regeneration processes, must be carefully analyzed in replicates in field trials. One remarkable challenge is related to the time lapse that selects the modified elite genotypes, because these strategies may spend a variable amount of time before the results are commercialized, where in all the cases it should be take into account the genotype × environment interactions. Furthermore, this combination of techniques can create an encouraging bridge between the public opinion and the community of geneticists who are concerned with plant genetic improvement. In this context, either transgenesis or genomic editing strategies become complementary modern tools to facing the challenges of plant genetic improvement. Their applications will depend on case-by-case analysis, and when possible will necessary associate them to the schemes and bases of classic plant genetic improvement. How to cite: Arencibia A, D'Afonseca V, Chakravarthi M, et al. Learning from transgenics: Advanced gene editing technologies should also bridge the gap with traditional genetic selection. Electron J Biotechnol 2019;41. https://doi.org/10.1016/j.ejbt.2019.06.00
Metodo di bioreattore ad immersione temporanea e relativo prodotto - ITRE2012A000080
No full textUn metodo di bioreattore ad immersione temporanea ed un prodotto ottenuto con tale metodo, il metodo comprendendo le fasi di: a. collocare una o più piante in grado di eseguire la fotosintesi all'interno di un contenitore; b. introdurre nel contenitore un terreno liquido, in modo da immergere le piante e lasciarle in immersione per un lasso di tempo in modo da moltiplicare le piante e produrre biomassa vegetale; c. rimuovere il terreno liquido dal contenitore e lasciare le piante in una atmosfera di aria con aggiunta di anidride carbonica per un altro lasso di tempo; d. ripetere le fasi b. e c. in modo alternato per un determinato periodo di tempo complessivo, sottoponendo le piante ad una radiazione fotosinteticamente attiva
A hybrid cloud computing approach for intelligent processing and storage of scientific data
No full textThis paper covers a new approach on Cloud Computing for Scientific Data Storage and Processing using a hybrid system with both on-site and on-the-cloud systems. The system analyzes use cases which are not resolved by either one of the single systems themselves.1881820,329Q
Histological and ultrastructural analysis of A. rhizogenes-mediated root formation in walnut cuttings
No full textThis chapter discusses the histological and ultrastructural changes occurring in the agroinfected cuttings of a recalcitrant walnut genotype cultured under conditions inductive for rooting to determine the way agrobacteria trigger root formation in the stem of a recalcitrant woody cutting, their migration in the explant, and the cytological events resulting from the combined presence of infection and exogenous auxin. The induction of adventitious roots is crucial for vegetative propagation in plant or micropropagation in vitro of many woody species, yet many woody species are totally recalcitrant to rooting. This recalcitrance may be associated with various histologically detectable factors, such as the inability of the explant cells after stimulation to organize root meristemoids. In English walnut (J. regia L.), the propagation by grafting on seedling rootstocks is an expensive process, yet the alternative practice of grafting on black walnut seedling rootstocks makes trees vulnerable to the lethal blackline disease. In vitro, root formation may be a response to wounding per se, or it may be associated with the presence of root inducers, such as auxin
Numerical analysis of a 3-D printed porous trailing edge for broadband noise reduction
No full textLattice Boltzmann simulations were carried out to investigate the noise mitigation mechanisms of a 3-D printed porous trailing-edge insert, elucidating the link between noise reduction and material permeability. The porous insert is based on a unit cell resembling a lattice of diamond atoms. It replaces the last 20 % chord of a NACA 0018 at zero angle-of-attack. A partially blocked insert is considered by adding a solid partition between 84 % and 96 % of the aerofoil chord. The regular porous insert achieves a substantial noise reduction at low frequencies, although a slight noise increase is found at high frequencies. The partially blocked porous insert exhibits a lower noise reduction level, but the noise emission at mid-to-high frequency is slightly affected. The segment of the porous insert near the tip plays a dominant role in promoting noise mitigation, whereas the solid-porous junction contributes, in addition to the rough surface, towards the high-frequency excess noise. The current study demonstrates the existence of an entrance length associated with the porous material geometry, which is linked to the pressure release process that is responsible for promoting noise mitigation. This process is characterised by the aerodynamic interaction between pressure fluctuations across the porous medium, which is found at locations where the porous insert thickness is less than twice the entrance length. Present results also suggest that the noise attenuation level is related to both the chordwise extent of the porous insert and the streamwise turbulent length scale. The porous inserts also cause a slight drag increase compared to their solid counterpart. Wind Energ
METODO DI BIOREATTORE AD IMMERSIONE TEMPORANEA E RELATIVO PRODOTTO
No full textA temporary immersion bioreactor method and a product obtained therefrom, the method comprising steps of: a. inserting one or more plants (200) capable of enacting photosynthesis into a container (110); b. inserting a liquid medium (205) into the container (110), such as to immerse the plants (200), leaving the plants (200) immersed for a period of time such as to multiply the plants and grow vegetable biomass; c. removing the liquid medium (205) from the container (110) and leaving the plants (200) in an air atmosphere with added carbon dioxide for a further period of time; d. repeating steps b. and c. in alternation for a predefined total period of time, exposing the plants (200) to a photosynthetically-active radiation
A TEMPORARY IMMERSION BIOREACTOR METHOD AND RELATIVE PRODUCT
No full textA temporary immersion bioreactor method and a product obtained therefrom, the method comprising steps of: a. in serting one or more plants (200) capable of enacting photosynthesis into a container ( 110); b. inserting a liquid medium (205) into the container ( 110), such as to immerse the plants (200), leaving the plants (200) immersed for a period of time such as to multiply o the plants and grow vegetable biomass; c. removing the liquid medium (205) from the container ( 110) and leaving the plants (200) in an air atmosphere with added carbon dioxide for a further period of time; d. repeating steps b. and c. in alternation for a predefined total period of time, exposing the plants (200) to a photosynthetically-active radiation
A 2 h periodic variation in the low-mass X-ray binary Ser X-1
No full textSpectroscopy of the low-mass X-ray binary Ser X-1 using the Gran Telescopio Canarias have revealed a ?2 h periodic variability that is present in the three strongest emission lines. We tentatively interpret this variability as due to orbital motion, making it the first indication of the orbital period of Ser X-1. Together with the fact that the emission lines are remarkably narrow, but still resolved, we show that a main-sequence K dwarf together with a canonical 1.4 M? neutron star gives a good description of the system. In this scenario, the most likely place for the emission lines to arise is the accretion disc, instead of a localized region in the binary (such as the irradiated surface or the stream-impact point), and their narrowness is due instead to the low inclination (?10°) of Ser X-1
Web Applications: A Proposal to Improve Response Time and Its Application to MOODLE
No full textThis paper covers some of the most advanced optimization techniques for web servers and web applications applied to a Modular Object Oriented Distance Learning Environment based on PHP 5 and Apache 2.22521
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