1,721,048 research outputs found
Primary Structure and Reactive Site of a Novel Wheat Proteinase Inhibitor of Subtilisin and Chymotrypsin
No full textThe effects of ohmic and conventional blanching on the nutritional, bioactive compounds and quality parameters of artichoke heads
No full textThe effect of ohmic (24 V/cm, 80°C) and conventional hot water (100°C) blanching on peroxidase (POD) and polyphenol oxidase (PPO) inactivation, colour parameters [L, hue (h), Chroma (C), E*], texture (maximum compression force) as well as total protein and phenolic content of artichoke heads immediately after blanching as well as during storage by canning was investigated.
The experimental results have shown that ohmic blanching inactivates both enzymes at lower processing times than conventional blanching, with total inactivation times of 360 s and 480 s, respectively. The colour of the raw product was well preserved after ohmic heating, while after water blanching it changed significantly from a yellow/green to a brownish/green. In addition, when compared to the artichokes blanched by ohmic treatment, the samples treated by the conventional method appeared heterogeneously softened showing a lower softening in the inner region but excessive softening in the outer part.
Finally, the results have revealed that the blanching method also affects the nutritional and bioactive compounds in artichokes immediately after blanching and during storage by canning, with the losses in both protein and polyphenolic content in conventionally pre-treated samples being greater than those measured in samples pre-treated by ohmic blanching
Primary Structure and Reactive Site of a Novel Wheat Proteinase Inhibitor of Subtilisin and Chymotrypsin
No full textEnhanced cytotoxic activity of a bifunctional chimeric protein containing a type 1 ribosome-inactivating protein and a serine protease inhibitor
No full textBoth ribosome-inactivating proteins (RIPs) and plant proteinase inhibitors, belong to protein families known to regulate cellular homeostasis and likely involved in plant defense. Nevertheless the interest in these protein classes is due to their potential use for the treatment of several important human diseases such as cancer. Thus, in the present study, type 1 ribosome- inactivating protein and wheat subtilisin/chymotrypsin inhibitor, were engineered into a chimeric protein with cytotoxic action selective for murine tumor cells, while lacking any appreciable toxicity on murine normal cells. This chimeric protein selectively sensitizes to apoptotic death cells derived from Simian-virus-40-transformed mouse fibroblasts (SVT2 cells). The cytotoxicity of this new recombinant product has been detected also on three different human malignant cells. Therefore action on tumor cells of this protein could represent a potentially very attractive novel tool for anticancer drug design. © 2012 Published by Elsevier Masson SAS. All rights reserve
An intron-less βγ-crystallin-type gene from the sponge Geodia cydonium
No full textWe report the cloning of a gene encoding a βγ-crystallin-type protein from a porifera, the Geodia cydonium sponge. The data provide direct, conclusive evidence of the existence of such a gene in the genome of an early diverged metazoan. The cloned gene is found to contain no introns, while proto-splice sites are identified in the nucleotide sequence at positions where introns are located in homologous, very recently diverged vertebrate genes. These findings are discussed in the light of the debate between the introns-late and introns-early theories. © 2002 Elsevier Science B.V. All rights reserved
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Valle Agricola Chickpeas: Mineral content of a Typical Landrace Legume from Southern Italy
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