312 research outputs found
An overview of the ATLAS High Level Trigger Dataflow and Supervision.
No full textThe ATLAS high-level trigger (HLT) system provides software-based event selection after the initial LVL1 hardware trigger. It is composed of two stages, the LVL2 trigger and the event filter (EF). The LVL2 trigger performs event selection with optimized algorithms using selected data guided by Region of Interest pointers provided by the LVL1 trigger. Those events selected by LVL2 are built into complete events, which are passed to the EF for a further stage of event selection and classification using off-line algorithms. Events surviving the EF selection are passed for off-line storage. The two stages of HLT are implemented on processor farms. The concept of distributing the selection process between LVL2 and EF is a key element in the architecture, which allows it to be flexible to changes (luminosity, detector knowledge, background conditions, etc.) Although there are some differences in the requirements between these subsystems there are many commonalities. An overview of the dataflow (event selection) and supervision (control, configuration, monitoring) activities in the HLT is given, highlighting where commonalities between the two subsystems can be exploited and indicating where requirements dictate that implementations differ. An HLT prototype system has been built at CERN. Functional testing is being carried out in order to validate the HLT architecture
Effect Handler Oriented Programming for Data Processing Applications
No full textEffect handler oriented programming or EHOP for short, is a new programming paradigm aiming to achieve separation of concerns in code which will lead to modular, readable and maintainable code. Since EHOP is significantly new, it is important to assess and compare it against traditional, commonly used paradigms in order to see if a wider adoption of EHOP would prove beneficial to computer science. In this research, EHOP was compared with traditional paradigms under the context of data processing applications. An Excel-like command line application called “MiniExcel” was implemented from scratch. Moreover, “Hierarchical EHOP”, a new structural pattern for EHOP was defined which enforces rules between concepts and produces a readable code structure. The main conclusions of this research can be summarized by the following statements. EHOP produces more modular, readable and maintainable code compared to traditional paradigms. Implementing additional concepts and updates to code is seamless using EHOP, yet the lack of development in EHOP’s ecosystem raises frustrating errors and requires the developer to implement libraries that are usually built-in for languages that support traditional paradigms. Functional programming produces faster running code, but EHOP is more memory efficient. Therefore, for applications that interact with users EHOP is the better choice and for applications that only execute code functional programming is more suitable.https://github.com/alibasaran/EHOP-Excel/ The codebase containing the implemented application as well as scripts that were used in programming paradigm analysis.CSE3000 Research ProjectComputer Science and Engineerin
Segmentation of isotropic 3D electron microscopy (FIB-SEM) data of mouse double-innervated Meissner corpuscle (jrc_mus-meissner-corpuscle-2)
No full textSample: Double-innervated Meissner corpuscle from the forepaw of a 3-week-old C57BL/6J WT mouseSample Description: Across mammalian skin, structurally complex and diverse mechanosensory end organs respond to mechanical stimuli and enable our perception of dynamic, light touch. How forces act on morphologically dissimilar mechanosensory end organs of the skin to gate the requisite mechanotransduction channel Piezo2 and excite mechanosensory neurons is not understood. Here, we report high-resolution reconstructions of the hair follicle lanceolate complex, Meissner corpuscle, and Pacinian corpuscle and the subcellular distribution of Piezo2 within them. Across all three end organs, Piezo2 is restricted to the sensory axon membrane, including axon protrusions that extend from the axon body. These protrusions, which are numerous and elaborate extensively within the end organs, tether the axon to resident non-neuronal cells via adherens junctions. These findings support a unified model for dynamic touch in which mechanical stimuli stretch hundreds to thousands of axon protrusions across an end organ, opening proximal, axonal Piezo2 channels and exciting the neuron.This dataset contains manually proofread automatic segmentation of the FIB-SEM dataset in jrc_mus-meissner-corpuscle-2.Protocol: Samples were dissected and drop fixed in glutaraldehyde and paraformaldehyde, and then osmicated with osmium tetroxide and potassium ferrocyanide, followed by osmium tetroxide only. Samples were subsequently stained with uranyl acetate and samarium chloride. Samples were dehydrated with an ethanol series followed by anhydrous acetone, infiltrated with Durcupan resin, and cured at 60°C.Contributions: Sample provided by Annie Handler (Harvard Medical School/HHMI) and Qiyu Zhang (Harvard Medical School/HHMI), prepared for imaging by Song Pang (HHMI/Janelia, currently at Yale School of Medicine), imaged by Song Pang and C. Shan Xu (HHMI/Janelia, currently at Yale School of Medicine), post data registration by C. Shan Xu, global image alignment and processing by Annie Handler and Qiyu Zhang, automatic segmentation by Tri M. Nguyen (Harvard Medical School) under the supervision of Wei-Chung Allen Lee (Harvard Medical School), ground truth annotation by Rebecca Plumb, Brianna Sanchez, Karyl Ashjian, Aria Shotland, Bartianna Brown, Madiha Kabeer, Nusrat Africawala, Stuart Cattel, Annie Handler, and Qiyu Zhang (all Harvard Medical School/HHMI), and segmentation proofreading by Annie Handler, Qiyu Zhang, and Michael Nolan-Tamariz (Harvard Medical School/HHMI).Acquisition ID: jrc_mus-meissner-corpuscle-2Voxel size (nm): 6 x 6 x 6 (x, y, z)Data dimensions (µm): 74.1 x 55.1 x 69.6 (x, y, z)Scanning speed (MHz): 1Dataset URL (Redirect): https://data.janelia.org/gTkdEEM DOI: https://doi.org/10.25378/janelia.23969106Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-meissner-corpuscle-2Publication: Handler et al., 2023</p
Isotropic 3D electron microscopy (FIB-SEM) data of mouse double-innervated Meissner corpuscle (jrc_mus-meissner-corpuscle-2)
No full textSample: Double-innervated Meissner corpuscle from the forepaw of a 3-week-old C57BL/6J WT mouseSample Description: Across mammalian skin, structurally complex and diverse mechanosensory end organs respond to mechanical stimuli and enable our perception of dynamic, light touch. How forces act on morphologically dissimilar mechanosensory end organs of the skin to gate the requisite mechanotransduction channel Piezo2 and excite mechanosensory neurons is not understood. Here, we report high-resolution reconstructions of the hair follicle lanceolate complex, Meissner corpuscle, and Pacinian corpuscle and the subcellular distribution of Piezo2 within them. Across all three end organs, Piezo2 is restricted to the sensory axon membrane, including axon protrusions that extend from the axon body. These protrusions, which are numerous and elaborate extensively within the end organs, tether the axon to resident non-neuronal cells via adherens junctions. These findings support a unified model for dynamic touch in which mechanical stimuli stretch hundreds to thousands of axon protrusions across an end organ, opening proximal, axonal Piezo2 channels and exciting the neuron.This dataset contains the FIB-SEM data of a double-innervated Meissner corpuscle from the forepaw of a 3-week-old C57BL/6J mouse.Protocol: Samples were dissected and drop fixed in glutaraldehyde and paraformaldehyde, and then osmicated with osmium tetroxide and potassium ferrocyanide, followed by osmium tetroxide only. Samples were subsequently stained with uranyl acetate and samarium chloride. Samples were dehydrated with an ethanol series followed by anhydrous acetone, infiltrated with Durcupan resin, and cured at 60°C.Contributions: Sample provided by Annie Handler (Harvard Medical School/HHMI) and Qiyu Zhang (Harvard Medical School/HHMI), prepared for imaging by Song Pang (HHMI/Janelia, currently at Yale School of Medicine), imaged by Song Pang and C. Shan Xu (HHMI/Janelia, currently at Yale School of Medicine), post data registration by C. Shan Xu, global image alignment and processing by Annie Handler and Qiyu Zhang, automatic segmentation by Tri M. Nguyen (Harvard Medical School) under the supervision of Wei-Chung Allen Lee (Harvard Medical School), ground truth annotation by Rebecca Plumb, Brianna Sanchez, Karyl Ashjian, Aria Shotland, Bartianna Brown, Madiha Kabeer, Nusrat Africawala, Stuart Cattel, Annie Handler, and Qiyu Zhang (all Harvard Medical School/HHMI), and segmentation proofreading by Annie Handler, Qiyu Zhang, and Michael Nolan-Tamariz (Harvard Medical School/HHMI).Acquisition ID: jrc_mus-meissner-corpuscle-2Voxel size (nm): 6 x 6 x 6 (x, y, z)Data dimensions (µm): 74.1 x 55.1 x 69.6 (x, y, z)Scanning speed (MHz): 1Dataset URL (Redirect): https://data.janelia.org/GCyjJVisualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-meissner-corpuscle-2Publication: Handler et al., 2023</p
Segmentation of isotropic 3D electron microscopy (FIB-SEM) data of mouse guard hair follicle (jrc_mus-guard-hair-follicle)
No full textSample: Guard hair follicle from the back of a 3-week-old C57BL/6J WT mouseSample Description: Across mammalian skin, structurally complex and diverse mechanosensory end organs respond to mechanical stimuli and enable our perception of dynamic, light touch. How forces act on morphologically dissimilar mechanosensory end organs of the skin to gate the requisite mechanotransduction channel Piezo2 and excite mechanosensory neurons is not understood. Here, we report high-resolution reconstructions of the hair follicle lanceolate complex, Meissner corpuscle, and Pacinian corpuscle and the subcellular distribution of Piezo2 within them. Across all three end organs, Piezo2 is restricted to the sensory axon membrane, including axon protrusions that extend from the axon body. These protrusions, which are numerous and elaborate extensively within the end organs, tether the axon to resident non-neuronal cells via adherens junctions. These findings support a unified model for dynamic touch in which mechanical stimuli stretch hundreds to thousands of axon protrusions across an end organ, opening proximal, axonal Piezo2 channels and exciting the neuron.This dataset contains manually proofread automatic segmentation of the FIB-SEM dataset in jrc_mus-guard-hair-follicle.Protocol: Samples were dissected and drop fixed in glutaraldehyde and paraformaldehyde, and then osmicated with osmium tetroxide and potassium ferrocyanide, followed by osmium tetroxide only. Samples were subsequently stained with uranyl acetate and samarium chloride. Samples were dehydrated with an ethanol series followed by anhydrous acetone, infiltrated with Durcupan resin, and cured at 60°C.Contributions: Sample provided by Annie Handler (Harvard Medical School/HHMI) and Qiyu Zhang (Harvard Medical School/HHMI), prepared for imaging by Song Pang (HHMI/Janelia, currently at Yale School of Medicine), imaged by Song Pang and C. Shan Xu (HHMI/Janelia, currently at Yale School of Medicine), post data registration by C. Shan Xu, global image alignment and processing by Annie Handler and Qiyu Zhang, automatic segmentation by Tri M. Nguyen (Harvard Medical School) under the supervision of Wei-Chung Allen Lee (Harvard Medical School), ground truth annotation by Rebecca Plumb, Brianna Sanchez, Karyl Ashjian, Aria Shotland, Bartianna Brown, Madiha Kabeer, Nusrat Africawala, Stuart Cattel, Annie Handler, and Qiyu Zhang (all Harvard Medical School/HHMI), and segmentation proofreading by Annie Handler, Qiyu Zhang, and Michael Nolan-Tamariz (Harvard Medical School/HHMI).Acquisition ID: jrc_mus-guard-hair-follicleVoxel size (nm): 6 x 6 x 6 (x, y, z)Data dimensions (µm): 88.1 x 95.9 x 77.6 (x, y, z)Scanning speed (MHz): 1Dataset URL (Redirect): https://data.janelia.org/HBfInEM DOI: https://doi.org/10.25378/janelia.23969052Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-guard-hair-folliclePublication: Handler, et al., 2023</p
Isotropic 3D electron microscopy (FIB-SEM) data of mouse guard hair follicle (jrc_mus-guard-hair-follicle)
No full textSample: Guard hair follicle from the back of a 3-week-old C57BL/6J WT mouseSample Description: Across mammalian skin, structurally complex and diverse mechanosensory end organs respond to mechanical stimuli and enable our perception of dynamic, light touch. How forces act on morphologically dissimilar mechanosensory end organs of the skin to gate the requisite mechanotransduction channel Piezo2 and excite mechanosensory neurons is not understood. Here, we report high-resolution reconstructions of the hair follicle lanceolate complex, Meissner corpuscle, and Pacinian corpuscle and the subcellular distribution of Piezo2 within them. Across all three end organs, Piezo2 is restricted to the sensory axon membrane, including axon protrusions that extend from the axon body. These protrusions, which are numerous and elaborate extensively within the end organs, tether the axon to resident non-neuronal cells via adherens junctions. These findings support a unified model for dynamic touch in which mechanical stimuli stretch hundreds to thousands of axon protrusions across an end organ, opening proximal, axonal Piezo2 channels and exciting the neuron.This dataset contains the FIB-SEM data of a guard hair follicle from the back of a 3-week-old C57BL/6J mouse.Protocol: Samples were dissected and drop fixed in glutaraldehyde and paraformaldehyde, and then osmicated with osmium tetroxide and potassium ferrocyanide, followed by osmium tetroxide only. Samples were subsequently stained with uranyl acetate and samarium chloride. Samples were dehydrated with an ethanol series followed by anhydrous acetone, infiltrated with Durcupan resin, and cured at 60°C.Contributions: Sample provided by Annie Handler (Harvard Medical School/HHMI) and Qiyu Zhang (Harvard Medical School/HHMI), prepared for imaging by Song Pang (HHMI/Janelia, currently at Yale School of Medicine), imaged by Song Pang and C. Shan Xu (HHMI/Janelia, currently at Yale School of Medicine), post data registration by C. Shan Xu, global image alignment and processing by Annie Handler and Qiyu Zhang, automatic segmentation by Tri M. Nguyen (Harvard Medical School) under the supervision of Wei-Chung Allen Lee (Harvard Medical School), ground truth annotation by Rebecca Plumb, Brianna Sanchez, Karyl Ashjian, Aria Shotland, Bartianna Brown, Madiha Kabeer, Nusrat Africawala, Stuart Cattel, Annie Handler, and Qiyu Zhang (all Harvard Medical School/HHMI), and segmentation proofreading by Annie Handler, Qiyu Zhang, and Michael Nolan-Tamariz (Harvard Medical School/HHMI).Acquisition ID: jrc_mus-guard-hair-follicleVoxel size (nm): 6 x 6 x 6 (x, y, z)Data dimensions (µm): 88.1 x 95.9 x 77.6 (x, y, z)Scanning speed (MHz): 1Dataset URL (Redirect): https://data.janelia.org/b0v8GVisualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-guard-hair-folliclePublication: Handler et al., 2023</p
Isotropic 3D electron microscopy (FIB-SEM) data of mouse single-innervated Meissner corpuscle (jrc_mus-meissner-corpuscle-1)
No full textSample: Single-innervated Meissner corpuscle from the forepaw of a 3-week-old C57BL/6J WT mouseSample Description: Across mammalian skin, structurally complex and diverse mechanosensory end organs respond to mechanical stimuli and enable our perception of dynamic, light touch. How forces act on morphologically dissimilar mechanosensory end organs of the skin to gate the requisite mechanotransduction channel Piezo2 and excite mechanosensory neurons is not understood. Here, we report high-resolution reconstructions of the hair follicle lanceolate complex, Meissner corpuscle, and Pacinian corpuscle and the subcellular distribution of Piezo2 within them. Across all three end organs, Piezo2 is restricted to the sensory axon membrane, including axon protrusions that extend from the axon body. These protrusions, which are numerous and elaborate extensively within the end organs, tether the axon to resident non-neuronal cells via adherens junctions. These findings support a unified model for dynamic touch in which mechanical stimuli stretch hundreds to thousands of axon protrusions across an end organ, opening proximal, axonal Piezo2 channels and exciting the neuron.This dataset contains the FIB-SEM data of a single-innervated Meissner corpuscle from the forepaw of a 3-week-old C57BL/6J mouse.Protocol: Samples were dissected and drop fixed in glutaraldehyde and paraformaldehyde, and then osmicated with osmium tetroxide and potassium ferrocyanide, followed by osmium tetroxide only. Samples were subsequently stained with uranyl acetate and samarium chloride. Samples were dehydrated with an ethanol series followed by anhydrous acetone, infiltrated with Durcupan resin, and cured at 60°C.Contributions: Sample provided by Annie Handler (Harvard Medical School/HHMI) and Qiyu Zhang (Harvard Medical School/HHMI), prepared for imaging by Song Pang (HHMI/Janelia, currently at Yale School of Medicine), imaged by Song Pang and C. Shan Xu (HHMI/Janelia, currently at Yale School of Medicine), post data registration by C. Shan Xu, global image alignment and processing by Annie Handler and Qiyu Zhang, automatic segmentation by Tri M. Nguyen (Harvard Medical School) under the supervision of Wei-Chung Allen Lee (Harvard Medical School), ground truth annotation by Rebecca Plumb, Brianna Sanchez, Karyl Ashjian, Aria Shotland, Bartianna Brown, Madiha Kabeer, Nusrat Africawala, Stuart Cattel, Annie Handler, and Qiyu Zhang (all Harvard Medical School/HHMI), and segmentation proofreading by Annie Handler, Qiyu Zhang, and Michael Nolan-Tamariz (Harvard Medical School/HHMI).Acquisition ID: jrc_mus-meissner-corpuscle-1Voxel size (nm): 6 x 6 x 6 (x, y, z)Data dimensions (µm): 36.7 x 45.3 x 57.2 (x, y, z)Scanning speed (MHz): 1Dataset URL (Redirect): https://data.janelia.org/KCjtYVisualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-meissner-corpuscle-1Publication: Handler et al., 2023</p
Isotropic 3D electron microscopy (FIB-SEM) data of mouse Pacinian corpuscle (jrc_mus-pacinian-corpuscle)
No full textSample: Pacinian corpuscle from the fibular periosteum membrane of a 16-week-old mixed background Plp1-EGFP mouseSample Description: Across mammalian skin, structurally complex and diverse mechanosensory end organs respond to mechanical stimuli and enable our perception of dynamic, light touch. How forces act on morphologically dissimilar mechanosensory end organs of the skin to gate the requisite mechanotransduction channel Piezo2 and excite mechanosensory neurons is not understood. Here, we report high-resolution reconstructions of the hair follicle lanceolate complex, Meissner corpuscle, and Pacinian corpuscle and the subcellular distribution of Piezo2 within them. Across all three end organs, Piezo2 is restricted to the sensory axon membrane, including axon protrusions that extend from the axon body. These protrusions, which are numerous and elaborate extensively within the end organs, tether the axon to resident non-neuronal cells via adherens junctions. These findings support a unified model for dynamic touch in which mechanical stimuli stretch hundreds to thousands of axon protrusions across an end organ, opening proximal, axonal Piezo2 channels and exciting the neuron.This dataset contains the FIB-SEM data of a Pacinian corpuscle from the fibular periosteum membrane of a 16-week-old mixed background mouse.Protocol: Samples were dissected and drop fixed in glutaraldehyde and paraformaldehyde, and then osmicated with osmium tetroxide and potassium ferrocyanide, followed by osmium tetroxide only. Samples were subsequently stained with uranyl acetate and samarium chloride. Samples were dehydrated with an ethanol series followed by anhydrous acetone, infiltrated with Durcupan resin, and cured at 60°C.Contributions: Sample provided by Annie Handler (Harvard Medical School/HHMI) and Qiyu Zhang (Harvard Medical School/HHMI), prepared for imaging by Song Pang (HHMI/Janelia, currently at Yale School of Medicine), imaged by Song Pang and C. Shan Xu (HHMI/Janelia, currently at Yale School of Medicine), post data registration by C. Shan Xu, global image alignment and processing by Annie Handler and Qiyu Zhang, automatic segmentation by Tri M. Nguyen (Harvard Medical School) under the supervision of Wei-Chung Allen Lee (Harvard Medical School), ground truth annotation by Rebecca Plumb, Brianna Sanchez, Karyl Ashjian, Aria Shotland, Bartianna Brown, Madiha Kabeer, Nusrat Africawala, Stuart Cattel, Annie Handler, and Qiyu Zhang (all Harvard Medical School/HHMI), and segmentation proofreading by Annie Handler, Qiyu Zhang, and Michael Nolan-Tamariz (Harvard Medical School/HHMI).Acquisition ID: jrc_mus-pacinian-corpuscleVoxel size (nm): 6 x 6 x 6 (x, y, z)Data dimensions (µm): 58.0 x 62.5 x 291.1 (x, y, z)Scanning speed (MHz): 3,2,1Dataset URL (Redirect): https://data.janelia.org/zzlhQVisualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-pacinian-corpusclePublication: Handler et al., 2023</p
Segmentation of isotropic 3D electron microscopy (FIB-SEM) data of mouse single-innervated Meissner corpuscle (jrc_mus-meissner-corpuscle-1)
No full textSample: Single-innervated Meissner corpuscle from the forepaw of a 3-week-old C57BL/6J WT mouseSample Description: Across mammalian skin, structurally complex and diverse mechanosensory end organs respond to mechanical stimuli and enable our perception of dynamic, light touch. How forces act on morphologically dissimilar mechanosensory end organs of the skin to gate the requisite mechanotransduction channel Piezo2 and excite mechanosensory neurons is not understood. Here, we report high-resolution reconstructions of the hair follicle lanceolate complex, Meissner corpuscle, and Pacinian corpuscle and the subcellular distribution of Piezo2 within them. Across all three end organs, Piezo2 is restricted to the sensory axon membrane, including axon protrusions that extend from the axon body. These protrusions, which are numerous and elaborate extensively within the end organs, tether the axon to resident non-neuronal cells via adherens junctions. These findings support a unified model for dynamic touch in which mechanical stimuli stretch hundreds to thousands of axon protrusions across an end organ, opening proximal, axonal Piezo2 channels and exciting the neuron.This dataset contains manually proofread automatic segmentation of the FIB-SEM dataset in jrc_mus-meissner-corpuscle-1.Protocol: Samples were dissected and drop fixed in glutaraldehyde and paraformaldehyde, and then osmicated with osmium tetroxide and potassium ferrocyanide, followed by osmium tetroxide only. Samples were subsequently stained with uranyl acetate and samarium chloride. Samples were dehydrated with an ethanol series followed by anhydrous acetone, infiltrated with Durcupan resin, and cured at 60°C.Contributions: Sample provided by Annie Handler (Harvard Medical School/HHMI) and Qiyu Zhang (Harvard Medical School/HHMI), prepared for imaging by Song Pang (HHMI/Janelia, currently at Yale School of Medicine), imaged by Song Pang and C. Shan Xu (HHMI/Janelia, currently at Yale School of Medicine), post data registration by C. Shan Xu, global image alignment and processing by Annie Handler and Qiyu Zhang, automatic segmentation by Tri M. Nguyen (Harvard Medical School) under the supervision of Wei-Chung Allen Lee (Harvard Medical School), ground truth annotation by Rebecca Plumb, Brianna Sanchez, Karyl Ashjian, Aria Shotland, Bartianna Brown, Madiha Kabeer, Nusrat Africawala, Stuart Cattel, Annie Handler, and Qiyu Zhang (all Harvard Medical School/HHMI), and segmentation proofreading by Annie Handler, Qiyu Zhang, and Michael Nolan-Tamariz (Harvard Medical School/HHMI).Acquisition ID: jrc_mus-meissner-corpuscle-1Voxel size (nm): 6 x 6 x 6 (x, y, z)Data dimensions (µm): 36.7 x 45.3 x 57.2 (x, y, z)Scanning speed (MHz): 1Dataset URL (Redirect): https://data.janelia.org/fnQYKEM DOI: https://doi.org/10.25378/janelia.23969070Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-meissner-corpuscle-1Publication: Handler et al., 2023</p
Segmentation of isotropic 3D electron microscopy (FIB-SEM) data of mouse Pacinian corpuscle (jrc_mus-pacinian-corpuscle)
No full textSample: Pacinian corpuscle from the fibular periosteum membrane of a 16-week-old mixed background Plp1-EGFP mouseSample Description: Across mammalian skin, structurally complex and diverse mechanosensory end organs respond to mechanical stimuli and enable our perception of dynamic, light touch. How forces act on morphologically dissimilar mechanosensory end organs of the skin to gate the requisite mechanotransduction channel Piezo2 and excite mechanosensory neurons is not understood. Here, we report high-resolution reconstructions of the hair follicle lanceolate complex, Meissner corpuscle, and Pacinian corpuscle and the subcellular distribution of Piezo2 within them. Across all three end organs, Piezo2 is restricted to the sensory axon membrane, including axon protrusions that extend from the axon body. These protrusions, which are numerous and elaborate extensively within the end organs, tether the axon to resident non-neuronal cells via adherens junctions. These findings support a unified model for dynamic touch in which mechanical stimuli stretch hundreds to thousands of axon protrusions across an end organ, opening proximal, axonal Piezo2 channels and exciting the neuron.This dataset contains manually proofread automatic segmentation of the FIB-SEM dataset in jrc_mus-pacinian-corpuscle.Protocol: Samples were dissected and drop fixed in glutaraldehyde and paraformaldehyde, and then osmicated with osmium tetroxide and potassium ferrocyanide, followed by osmium tetroxide only. Samples were subsequently stained with uranyl acetate and samarium chloride. Samples were dehydrated with an ethanol series followed by anhydrous acetone, infiltrated with Durcupan resin, and cured at 60°C.Contributions: Sample provided by Annie Handler (Harvard Medical School/HHMI) and Qiyu Zhang (Harvard Medical School/HHMI), prepared for imaging by Song Pang (HHMI/Janelia, currently at Yale School of Medicine), imaged by Song Pang and C. Shan Xu (HHMI/Janelia, currently at Yale School of Medicine), post data registration by C. Shan Xu, global image alignment and processing by Annie Handler and Qiyu Zhang, automatic segmentation by Tri M. Nguyen (Harvard Medical School) under the supervision of Wei-Chung Allen Lee (Harvard Medical School), ground truth annotation by Rebecca Plumb, Brianna Sanchez, Karyl Ashjian, Aria Shotland, Bartianna Brown, Madiha Kabeer, Nusrat Africawala, Stuart Cattel, Annie Handler, and Qiyu Zhang (all Harvard Medical School/HHMI), and segmentation proofreading by Annie Handler, Qiyu Zhang, and Michael Nolan-Tamariz (Harvard Medical School/HHMI).Acquisition ID: jrc_mus-pacinian-corpuscleVoxel size (nm): 12 x 12 x 12 (X, Y, Z)Data dimensions (µm): 58.0 x 62.5 x 291.1 (X, Y, Z)Scanning speed (MHz): 3,2,1Dataset URL (Redirect): https://data.janelia.org/aNkg3EM DOI: https://doi.org/10.25378/janelia.23969112Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-pacinian-corpusclePublications: Handler et al., 2023</p
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