37 research outputs found
First person – Annalisa Pinsino and Andi Alijagic
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Annalisa Pinsino and Andi Alijagic are co-first authors on ‘Sea urchin Paracentrotus lividus immune cells in culture: formulation of the appropriate harvesting and culture media and maintenance conditions’, published in BIO. Annalisa is a Third-class Researcher in her lab at Consiglio Nazionale delle Ricerche, Istituto di Biomedicina e Immunologia Molecolare ‘A. Monroy’, Palermo, Italy, investigating probing safety of nanoparticles by outlining sea urchin innate signalling pathways in vitro. Andi is a PhD Student in the lab of Dr Annalisa Pinsino
Sea urchin Paracentrotus lividus immune cells in culture: formulation of the appropriate harvesting and culture media and maintenance conditions
No full textThe sea urchin is an emergent model system for studying basic and translational immunology. Here we report a new method for the harvesting and maintenance of primary immune cells isolated from adult Paracentrotus lividus, a common Mediterranean sea urchin species. This optimised method uses coelomocyte culture medium, containing a high-affinity Ca2+ chelator, as the ideal harvesting and anti-clotting vehicle and short-term culture medium (≤48 h), and artificial seawater as the master medium that maintains cell survival and in vitro-ex vivo physiological homeostasis over 2 weeks. Gradually reducing the amount of anticoagulant solution in the medium and regularly replacing the medium led to improved culture viability. Access to a robust and straightforward in vitro-ex vivo system will expedite our understanding of deuterostome immunity as well as underscore the potential of sea urchin with respect to biomedicine and regulatory testing. This article has an associated First Person interview with the first author of the paper
Semi-separability and Operators of Mixed Causality
No full textElectrical Engineering, Mathematics and Computer Scienc
Probing safety of nanoparticles by outlining sea urchin sensing and signaling cascades
No full textAmong currently identified issues presenting risks and benefits to human and ocean health, engineered nanoparticles (ENP) represent a priority. Predictions of their economic and social impact appear extraordinary, but their release in the environment at an uncontrollable rate is in striking contrast with the extremely limited number of studies on environmental impact, especially on the marine environment. The sea urchin has a remarkable sensing environmental system whose function and diversity came into focus during the recent years, after sea urchin genome sequencing. The complex immune system may be the basis wherefore sea urchins can adapt to a dynamic environment and survive even in hazardous conditions both in the adult and in the embryonic life. This review is aimed at discussing the literature in nanotoxicological/ecotoxicological studies with a focus on stress and innate immune signaling in sea urchins. In addition, here we introduce our current development of in vitro-driven probes that could be used to dissect ENP aftermaths, suggesting their future use in immune-nanotoxicology.</p
Dielectric properties and space charge dynamics of polymeric high voltage DC insulating materials
No full textElectrical Engineering, Mathematics and Computer Scienc
The health and environmental challenges of recycling chemicals in the sustainable management of plastics
No full textKonferens:Uppsala Health Summit: Chemical Pollution and One Health - From Reactivity to Proactivity, 24-25 October 2023</p
The health and environmental challenges of recycling chemicals in the sustainable management of plastics
No full textKonferens:Uppsala Health Summit: Chemical Pollution and One Health - From Reactivity to Proactivity, 24-25 October 2023</p
The health and environmental challenges of recycling chemicals in the sustainable management of plastics [Elektronisk resurs]
No full textThe health and environmental challenges of recycling chemicals in the sustainable management of plastics
No full textKonferens:Uppsala Health Summit: Chemical Pollution and One Health - From Reactivity to Proactivity, 24-25 October 2023</p
Sea Urchin Extracellular Proteins Design a Complex Protein Corona on Titanium Dioxide Nanoparticle Surface Influencing Immune Cell Behavior
No full textExtensive exploitation of titanium dioxide nanoparticles (TiO2NPs) augments rapid release into the marine environment. When in contact with the body fluids of marine invertebrates, TiO2NPs undergo a transformation and adhere various organic molecules that shape a complex protein corona prior to contacting cells and tissues. To elucidate the potential extracellular signals that may be involved in the particle recognition by immune cells of the sea urchin Paracentrotus lividus, we investigated the behavior of TiO2NPs in contact with extracellular proteins in vitro. Our findings indicate that TiO2NPs are able to interact with sea urchin proteins in both cell-free and cell-conditioned media. The two-dimensional proteome analysis of the protein corona bound to TiO2NP revealed that negatively charged proteins bound preferentially to the particles. The main constituents shaping the sea urchin cell-conditioned TiO2NP protein corona were proteins involved in cellular adhesion (Pl-toposome, Pl-galectin-8, Pl-nectin) and cytoskeletal organization (actin and tubulin). Immune cells (phagocytes) aggregated TiO2NPs on the outer cell surface and within well-organized vesicles without eliciting harmful effects on the biological activities of the cells. Cells showed an active metabolism, no oxidative stress or caspase activation. These results provide a new level of understanding of the extracellular proteins involved in the immune-TiO2NP recognition and interaction in vitro, confirming that primary immune cell cultures from P. lividus can be an optional model for swift and efficient immune-toxicological investigations. </p
