187,185 research outputs found
“Use of Psycodynamic oriented Group Counselling to prevent homophobic bullying”
No full textIl capitolo descrive l'utilizzo del counselling di gruppo psicodinamicamente orientato come strumento di prevenzione a dinamiche di bullismo e bullismo omofobico
“Feel, experiment, act: an action research to prevent and contrast homophobic bullying in schools”,
No full textIl capitolo descrive un'esperienza di ricerca intervento condotta in una scuola secondaria di secondo grado di Napoli. L'obbiettivo dell'intervento era di contrastare e prevenire le molteplici forme che assume la discriminazione e la violenza scolastica, in particolare quella di marca sessista e omofobica. Nel lavoro è descritto il metodo di intervento, metodo che combinava tecniche di matrice psicodinamica e sociocognitiva
Structural determinants of salmon calcitonin bioactivity -the role of the leu-based amphipatic alfa-helix
No full textSalmon calcitonin (sCT) forms an amphipathic helix in the region 9-19, with the C-terminal decapeptide interacting with the helix (Amodeo, P., Motta, A., Strazzullo, G., Castiglione Morelli, M. A. (1999) J. Biomol. NMR 13, 161-174). To uncover the structural requirements for the hormone bioactivity, we investigated several sCT analogs. They were designed so as to alter the length of the central helix by removal and/or replacement of flanking residues and by selectively mutating or deleting residues inside the helix. The helix content was assessed by circular dichroism and NMR spectroscopies; the receptor binding affinity in human breast cancer cell line T 47D and the in vivo hypocalcemic activity were also evaluated. In particular, by NMR spectroscopy and molecular dynamics calculations we studied Leu(23),Ala(24)-sCT in which Pro(23) and Arg(24) were replaced by helix inducing residues. Compared with sCT, it assumes a longer amphipathic alpha-helix, with decreased binding affinity and one-fifth of the hypocalcemic activity, therefore supporting the idea of a relationship between a definite helix length and bioactivity. From the analysis of other sCT mutants, we inferred that the correct helix length is located in the 9-19 region and requires long range interactions and the presence of specific regions of residues within the sequence for high binding affinity and hypocalcemic activity. Taken together, the structural and biological data identify well defined structural parameters of the helix for sCT bioactivity
A NEW GENERAL-FORM OF MOLECULAR-FORCE FIELDS - APPLICATION TO INTRARESIDUE AND INTERRESIDUE INTERACTIONS IN PEPTIDES
No full textConversion of Enkephalin and Dermorphin into δ‐Selective Opioid Antagonists by Single‐Residue Substitution
No full textThe properties of di- and tri-peptides containing 1,2,3,6tetrahydroisoquinoline-3-carboxyliacc id
(Tic) in second position suggest that the message domain of opioid peptides can be composed of
only two residues [Temussi, P. A., Salvadori, S., Amodeo, P., Guerrini, R., Tomatis, R., Lazarus, L.
H., Picone, D. & Tancredi, T. (1994) Biochem. Biophys. Res. Commun. 198, 933-9391. As a crucial
test of the possibility that the Tyr-Tic segment be a message domain in longer peptide sequences,
we have inserted it in the sequences of two typical opioid peptides: [Leulenkephalin, a non-selective
agonist, and dermorphin, a selective p agonist. Here we report the synthesis and biological activity
of [~-Tic’]enkephalin, [~-Tic~]dermorphi[n~,- Tic’]dermorphin carboxylic acid and [~-Tic’]dermorphin:
all [L-Tic’lpeptides were converted from agonists to &selective antagonists. The NMR conformational
study in a dimethylsulfoxide/water cryoprotective mixture at low temperature shows diagnostic
side-chain-side-chain NOES in the spectra of all [~-Tic’]peptides and hints that the 90”
arrangement of the the two aromatic rings found in the cis-Tyr-L-Tic moiety, typical of N-methyl
naltrindole and other 6-selective opiate antagonists, is responsible for the antagonist activity of all
these peptides
Introduzione
No full textIl presente volume nasce dalla sinergia tra associazioni, istituzioni e ONG sviluppata nell’ambito della XVII settimana d’azione contro i razzismi, organizzata dall’Ufficio Nazionale Antidiscriminazioni della Presidenza del Consiglio dei Ministri. Nel testo sono riportate le trascrizioni delle relazioni tenute da docenti, militanti e practitioners che si occupano di richieste di protezione internazionale per motivi di identità sessuale durante il Convegno “I Have a Dream – Studi e strumenti per il lavoro con migranti LGBTI”.
La prima sezione raccoglie gli interventi delle istituzioni a vari livelli e delle organizzazioni non governative, dalla Presidenza del Consiglio dei Ministri, alla Regione, fino al Comune e dell’Alto Commissariato delle Nazioni Unite per i Rifugiati. Gli interventi sono intervallati da commenti e riflessioni critiche.
La seconda sezione contiene gli studi e le ricerche sul tema delle migrazioni queer in vari settori scientifico-disciplinari, dal- la sociologia, all’antropologia al diritto. Pur affrontando il tema prevalentemente in relazione alle migrazioni forzate, quindi alle richieste di asilo, sono riportati anche i risultati di riflessioni teoriche ed evidenze empiriche su migranti di prima e seconda generazione, non necessariamente asilanti o rifugiati. Ogni saggio breve è corredato da quattro testi di riferimento bibliografico.
Nella terza sezione sono riportate le relazioni di persone migranti LGBTIQ attiviste e di figure professionali che lavorano negli sportelli per richiedenti la protezione internazionale per motivi SOGIGESC
A technique for the correction of long-term magnetic flux integrator drift
No full textIn the framework of magnetic measurements in particle accelerators, the problem of the drift due to integration of a voltage offset causes a lack of stability, which is inadequate for long-term measurements. In this paper, a new approach for the modelling and correction of long-term drift in integrators is discussed. In particular, a feed-forward drift correction method has been developed analytically and tested using experimental data taken from an existing real-time magnetic measurement system. Among all the sensors, NMR was chosen for the integration constant due to its accuracy (10 −6 ) and the Metrolab PT2026 NMR teslameter has been tested successfully as a novel kind of quasi-DC field marker. The combination of feed-forward correction and PT2026 as a marker will allow the future real-time magnetic measurement systems to meet the very strict specification of 1 μT field stability over durations of the order of minutes. In certain cases, such as in ELENA, this method offers a way to reduce by three orders of magnitude the drift-induced field error with respect to the existing system
Conversion of Enkephalin and Dermorphin Into Delta-selective Opioid Antagonists By Single-residue Substitution
No full textThe properties of di- and tri-peptides containing 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid (Tic) in second position suggest that the message domain of opioid peptides can be composed of only two residues [Temussi, P. A., Salvadori, S., Amodeo, P., Guerrini, R., Tomatis, R., Lazarus, L. H., Picone, D. gr Tancredi, T. (1994) Biochem. Biophys. Res. Commun. 198, 933-939]. As a crucial test of the possibility that the Sr-Tic segment be a message domain in longer peptide sequences, we have inserted it in the sequences of two typical opioid peptides: [Leu]enkephalin, a non-selective agonist, and dermorphin, a selective mu agonist. Here we report the synthesis and biological activity of [L-Tic(2)]enkephalin, [L-Tic(2)]dermorphin, [L-Tic(2)]dermorphin carboxylic acid and [D-Tic(2)]dermorphin: all [L-Tic(2)]peptides were converted from agonists to delta-selective antagonists. The NMR conformational study in a dimethylsulfoxide/water cryoprotective mixture at low temperature shows diagnostic side-chain - side-chain NOEs in the spectra of all [L-Tic(2)]peptides and hints that the 90 degrees arrangement of the the two aromatic rings found in the cis-Tyr-L-Tic moiety, typical of N-methyl naltrindole and other delta-selective opiate antagonists, is responsible for the antagonist activity of all these peptides
- …
