13 research outputs found
O que há entre o público e o privado: vitalidade e tensões na apropriação do estacionamento do Carrefour na região sul de Natal
No full textThe unexpected appropriation of free spaces, such as the Carrefour parking lot in the Southern Region of Natal / RN, has been a way of indicating the population’s constant search for alternatives to public spaces, which are increasingly permeated by fear. Therefore, it is questioned how the supermarket constitutes an attraction for the accomplishment of different activities, by various social segments, in the context of the city. The motivations that involve the choice of theme concern the interest aroused by the results of previous research developed by the author and her colleague Jessyca Alencar, in which he was part of the study cut. It is also justified by the absence of academic papers on the subject, and by the contribution to new forms of apprehension of the nature and meanings of spaces, associated to the recognition of human participation in this construction. Then, the objective is to understand the processes of resignification of that area considering aspects of safety and vitality. To reach it, the precepts of several authors, such as Raquel Tardin, Eugênio Queiroga, Paulo César Gomes, Ana Fani Carlos, Frederico de Holanda and Jane Jacobs are used. They and the other names covered define terms such as free space, spheres of life, appropriation, identity, place, urbanity, diversity and vitality. The empirical data collected for the development of the evidence were collected through maps, questionnaires, open interviews and field observations, with the aid of photographic records, social networks and websites. The results then tend to prove the hypothesis and the fulfillment of the main purpose of the study, demonstrating that the physical aspects of the site are essential for the commitment of the public in focus. Associated with the fear of the surroundings (and of the parking lot itself), the signs of vitality remain, but suffer from novelties that impose limits and give rise to new questions.A apropriação inesperada de espaços livres, como o estacionamento do Carrefour da Região Sul de Natal/RN, tem sido uma forma de indicar a constante procura da população por alternativas aos espaços públicos, cada vez mais permeados pelo medo. Questiona-se, portanto, como o supermercado se constitui em um atrativo para a realização de diferentes atividades, por variados segmentos sociais, no contexto da cidade. As motivações que envolvem a escolha do tema dizem respeito ao interesse despertado pelos resultados de pesquisa anterior desenvolvida pela autora e sua colega Jessyca Alencar, na qual ele fazia parte do recorte de estudo. Justifica-se também pela ausência de trabalhos acadêmicos sobre o assunto, e pela contribuição para novas formas de apreensão da natureza e significados dos espaços, associada ao reconhecimento da participação humana nessa construção. O objetivo, portanto, é compreender os processos de ressignificação daquela área considerando aspectos de segurança e vitalidade. Para alcançá-lo, são utilizados os preceitos de diversos autores, como Raquel Tardin, Eugênio Queiroga, Paulo César Gomes, Ana Fani Carlos, Frederico de Holanda e Jane Jacobs. Eles e os demais nomes abordados definem termos como espaço livre, esferas de vida, apropriação, identidade, lugar, urbanidade, diversidade e vitalidade. Os dados empíricos levantados para o desenvolvimento das comprovações foram coletados por meio de mapas, questionários, entrevistas abertas e observações de campo, com o auxílio de registros fotográficos, redes sociais e sites. Os resultados tendem, então, à comprovação da hipótese e ao cumprimento do propósito principal do estudo, demonstrando que os aspectos físicos do local são essenciais para o comprometimento do público em foco. Associados ao medo do entorno (e do próprio estacionamento), os indícios de vitalidade se mantêm, mas sofrem com novidades que impõem limites e propicia novos questionamentos
Oxidative stress as an underling mechanism of anticancer drugs cytotoxicity on human red blood cells' membrane
Get PDFThe aim of this study is to investigate the direct in vitro effects of anticancer drugs on red blood cells (RBCs) and to explore the underlying mechanism, mainly by measuring RBCs oxidative stress (OS) status. After RBCs direct contact with fourteen (14) anticancer drugs, several parameters were assessed including: cellular turbidity, methemoglobin (metHb) generation, released Hb and Hb stability. Moreover, intracellular Hb, considered as new molecular target of anticancer drugs, was quantified inside RBCs. MDA level, the main biomarker of OS, was simultaneously measured. The cellular turbidity reveled severe (docetaxel “TXT”, 0.03 ± 0.002), moderate (methotrexate “MTX”, 0.49 ± 0.009), or none (5-fluorouracil “5-FU”, 0.76 ± 0.029) membrane cytotoxicity (MC). An inverse relationship between cell concentration, released Hb and metHb content was obtained. High metHb generation, revealing intense OS, was also mostly expressed in paclitaxel “TXL” and etoposide “VP16”. Further, epirubicin “EPI” and “TXT” induced important oxidation of membrane lipids with 0.32 ± 0.014 and 0.26 ± 0.004, respectively. Also, MTX (0.17 ± 0.006) and doxorubicin “DOX” (0.32 ± 0.034) affected significantly Hb stability by a direct contact with molecule. These findings demonstrated that anticancer drugs have the ability to induce membrane damages by the exacerbation of OS through membrane lipid peroxidation and Hb oxidation even inside RBCs
Study and Characterization of H3PO4 Activated Carbons Prepared from Jujube Stones for the Treatment of Industrial Textile Effluents
No full textInternational audienceDyes are responsible for major environmental issues globally due to their toxicity, large-scale production, and extensive use in various industrial sectors. Pollution caused by hazardous dyes is mainly textile waste, which constantly discharged into the aquatic system, often causing harm humans affecting water quality. In recent years, removal of from wastewater has been a challenge. Numerous technologies methods have developed remove meet clean requirements. this study, effectiveness activated carbon prepared chemical activation jujube stones treatment was investigated. The effects concentration H3PO4 carbonization temperature on carbon’s properties were studied. Several physicochemical methods, including Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction, methylene blue index, Boehm titration, iodine index pH point zero charge, considered characterize produced adsorbents. To assess quality two studied effluents (Mustard Violet), following parameters used: biological oxygen demand (BOD), (COD), turbidity, suspended particles dissolved solids, before after with carbon. Untreated analysis revealed high values almost all parameters: andg
Study and Characterization of H3PO4 Activated Carbons Prepared from Jujube Stones for the Treatment of Industrial Textile Effluents
Get PDFDyes are responsible for major environmental issues globally due to their toxicity, largescale
production, and extensive use in various industrial sectors. Pollution caused by hazardous
dyes is mainly due to textile waste, which is constantly discharged into the aquatic system, often
causing harm to humans and affecting water quality. In recent years, the removal of dyes from
industrial textile wastewater has been a major challenge. Numerous technologies and methods
have been developed to remove dyes from wastewater and meet clean water requirements. In this
study, the effectiveness of activated carbon prepared by chemical activation of jujube stones for
textile wastewater treatment was investigated. The effects of the concentration of H3PO4 and the
carbonization temperature on the activated carbon’s properties were studied. Several physicochemical
methods, including Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy
(SEM), X-ray diffraction, methylene blue index, Boehm titration, iodine index and pH point of zero
charge, were considered to characterize the produced adsorbents. To assess the quality of the two
studied textile effluents (Mustard and Violet), the following parameters were used: biological oxygen
demand (BOD), chemical oxygen demand (COD), turbidity, suspended particles and dissolved solids,
before and after treatment with the produced activated carbon. Untreated wastewater analysis
revealed high values for almost all parameters: pH > 9, COD of 302.72 mg/L and 230.68 mg/L for
Mustard and Violet effluent, respectively. Both effluents from an industrial textile factory exhibited a
COD/BOD ratio higher than three, which restricts their biodegradability. Examination of the effect of
contact time and activated carbon dosage on the treatment of the two effluents showed that 4 g/L
of activated carbon and 60 min of contact time were sufficient for optimal treatment, resulting in
pollutant removal rates of 81.03 and 84.65% for the Violet and Mustard effluents, respectively. The
results of this research highlight the efficiency of activated carbon derived from jujube stones as a
cost-effective adsorbent for the treatment of real textile wastewater
Optimization of ultrasound-assisted extraction of bioactive compounds from Carthamus caeruleus L. rhizome: Integrating central composite design, Gaussian process regression, and multi-objective Grey Wolf optimization approaches
No full textpeer reviewedThe prediction of ultrasound-assisted extraction (UAE) for total phenolic content (TPC) and total flavonoid content (TFC) from Carthamus caeruleus L. rhizomes was conducted using a Gaussian process regression model (GPR) with a multi-objective Grey Wolf optimization approach (MOGWO). A central composite design (CCD) was employed first, examining ethanol concentration, temperature, time, and solvent-to-solid ratio as independent variables. TPC and TFC responses were analyzed under various conditions, revealing significant quadratic and interaction effects (p < 0.05). The GPR was then utilized to predict TPC and TFC, showing high accuracy with correlation coefficients near 1 and minimal root mean square error (RMSE) values. To simultaneously maximize TPC and TFC, the MOGWO was used in a multi-objective framework. Validation through CCD and GPR highlighted GPR's superior predictive accuracy. Optimal conditions (10 % ethanol, 40°C, 20 minutes sonication, and 50 mL g−1 solvent to solid ratio) showed significant discrepancies in CCD predictions but high accuracy in GPR predictions. An interactive tool predicts TPC and TFC using CCD and GPR models. Users input extraction parameters and receive predictions, with a GWO-based optimization module for optimal conditions. The interface enables model comparison, improves process understanding, and optimizes bioactive compound extraction
A Soft Tissue Technique for Treating Knee Hyperextension or Recurvatum: Posterior Oblique Ligament Advancement
No full textBackground: Genu recurvatum is a challenging disorder and can negatively affect knee biomechanics, as reflected in its role as a risk factor for knee ligament injury and poor outcomes following ligament reconstruction or arthroplasty. Indication: Pathological recurvatum should be addressed in the presence of a causative correctible structural abnormality, whether due to decreased posterior tibial slope or due to multiligament injury. We described a posterior oblique ligament (POL) advancement technique for the treatment of knee recurvatum. Technique Description: After completion of all concomitant procedures such as ligament reconstructions, the procedure is initiated with a medial approach. The POL is isolated with an inverted L-shaped dissection and mobilized. Mattress sutures are passed through the resulting POL flap, and are re-tensioned and fixed anteriorly and distally, with the knee close to extension, in order for the imbrication to restrain hyperextension. Results: Anecdotal evidence points to satisfactory control of knee recurvatum postoperatively, which can potentially avoid its deleterious effects in surgical outcomes. Discussion/Conclusion: We present a promising option in the treatment of pathological recurvatum, with favorable cost-efficacy, low morbidity relative to slope-altering osteotomy or posterolateral soft tissue procedures, and focused on the biomechanically demonstrated main restrictors to knee hyperextension. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication
Hybrid Repair of a Medial Meniscus Mid-Body Radial Tear
No full textBackground: Meniscal radial tears are associated with altered contact mechanics, as they have the potential to disrupt the meniscal hoop stress mechanism. In prior studies, radial tears are associated with higher grade of cartilage damage, as well as higher rate and severity of meniscal extrusion in knees with a radial tear relative to other types of tears. Improved understanding of the meniscus biological potential together with modern developments in surgical technique has paved the way for the current emphasis on repairing even radial tears. Indication: Large or complete radial tears of the meniscus without prohibitive joint space narrowing or severe cartilage damage. Technique Description: A hybrid technique of meniscal radial repair is described, combining (1) 2 transtibial pullout cinch-loop sutures, (2) 2 inside-out vertical rip-stop sutures and 2 oblique sutures in a “cross-tag” configuration, and (3) 1 horizontal mattress all-inside suture. The repair is biologically augmented with a notch marrow-venting procedure. Results: Recent evidence has demonstrated significant biomechanical benefit in terms of increased load to failure and construct strength when adding transtibial tunnel augmentation to radial repairs. In addition, rip-stop sutures decrease the chance of cut-out relative to nonreinforced repairs. Those biomechanical advancements are reflected in excellent patient-reported outcomes as well as healing rates following radial repair. Discussion/Conclusion: We present a hybrid technique encompassing the most biomechanically effective novel radial repair techniques, backed by excellent clinical outcomes following radial repair in the current literature. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication
Full-Thickness Peroneus Longus Tendon Autograft Harvest
No full textBackground: Despite extensive evidence of excellent biomechanical and clinical outcomes of ligament reconstructions with bone-patellar tendon-bone, hamstring tendon, and quadriceps tendon autografts, these are not without limitations, including knee-related donor site morbidity, decreased range of motion, and potential for anterior knee pain. Therefore, researchers have explored other options, including the peroneus longus (PL) autograft. Indication: Anterior cruciate ligament (ACL) reconstruction (both primary and revision), as well as multiligament reconstruction, particularly in areas where access to allografts is limited. Surgical Technique: A 3-cm longitudinal skin incision is placed 2 fingerbreadths (or 3 cm) proximal to the tip of the lateral malleolus, and 3 mm posterior to the fibula. The PL and peroneus brevis tendons are identified and isolated, followed by a tenodesis at the distal limit of the incision. The PL is whipstitched proximal to the tenodesis site, and then incised between the tenodesis and whipstitched portion. A closed tendon stripper is inserted and advanced proximally until the PL graft is released. Discussion/Conclusion: PL autograft harvest has been shown to generate thicker and longer grafts relative to the semitendinosus, with demonstrated efficacy in terms of both biomechanical and clinical outcomes. Current literature points largely to a good safety profile, although there is still conflicting evidence in terms of foot and ankle morbidity and no mid- to long-term follow-up data available. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form
From Green Chemistry to Healthy Environments: Silver Nanoparticles as a Dual Antioxidant and Antibacterial Agents for Advancing Biomedicine and Sustainable Wastewater Treatment
No full textThe green synthesis of silver nanoparticles (AgNPs) using plant extracts is an eco-friendly method with potential for biomedical and environmental applications. This study aims to synthesize silver nanoparticles (SO-AgNPs) using Salvia officinalis L. extract and evaluate their antioxidant and antibacterial properties, positioning them as candidates for applications in sustainable biomedicine and wastewater treatment. S. officinalis L. extract was used to synthesize AgNPs under optimized conditions, with a 10% extract/AgNO₃ ratio and a reaction time of 180 min. The SO-AgNPs were characterized using ATR-FTIR, XRD, SEM, DLS, and Zeta potential analysis. The antioxidant activity of the extract and SO-AgNPs was evaluated using ABTS+• and DPPH• radical scavenging assays. Antibacterial activity was tested against 11 bacterial strains and bacteria isolated from industrial effluent, with minimal inhibitory concentrations (MIC) determined for both the extract and SO-AgNPs. The SO-AgNPs demonstrated potent antioxidant activity, with IC₅₀ values of 0.233 mg/mL and 0.305 mg/mL in the ABTS+• assay, and 0.173 mg/mL and 0.185 mg/mL in the DPPH• assay for the extract and SO-AgNPs, respectively. Antibacterial testing showed MIC values of 0.25 mg/mL for SO-AgNPs and between 3.12 and 6.25 mg/mL for S. officinalis L. extract against E. coli, P. aeruginosa, A. baumannii, MRSA, B. cereus, and S. epidermidis. For bacteria isolated from industrial effluent, the MIC values were 0.125 mg/mL for SO-AgNPs and 0.5 mg/mL for the extract. This study highlights the dual antioxidant and antibacterial capabilities of S. officinalis L. extract and SO-AgNPs, demonstrating their potential for use in both biomedical and environmental applications, including wastewater treatment
Tibial-Sling Triangular Medial Collateral Ligament Reconstruction With Posterior Oblique Ligament Limb
No full textBackground: While most medial-sided knee injuries can be managed conservatively, high-grade injuries of the posteromedial corner have been reported to have less than optimal outcomes with conservative approaches, resulting in residual valgus and rotational instability. Consensus among experts postulates that treatment should be based on reconstructing both the superficial medial collateral ligament (sMCL) and the posterior oblique ligament (POL). We present a modified technique of a tibial-sling anatomic MCL reconstruction with a POL limb. Indications: High-grade injuries of the posteromedial corner, specifically injuries with medial joint gapping in extension or grade 3 medial-sided tears. Technique Description: The MCL is reconstructed with a semitendinosus autograft in a tibial sling, created by converging perpendicular bone tunnels at its anatomic tibial insertion, and fixed to a femoral tunnel in the isometric point just posterior and proximal to the medial epicondyle with the knee at 30° of flexion. That same single femoral tunnel will be shared by the two strands of the semitendinosus graft and a limb of gracilis autograft to reproduce the POL, passed through a tibial tunnel in the posteromedial margin of medial tibial condyle, and fixed in extension. Discussion/Conclusion: As recent evidence suggests that medial reconstruction should be preferred over repair, due to favorable complication and failure rates, we describe a technique that is both anatomic-based, reproducing the 2 main medial knee structures (sMCL, POL), and also cost-saving, with decreased need of fixation devices. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication
