152 research outputs found
Protocol to target a promoter region in human embryonic kidney cells using the CRISPR-dCas9 system for single-locus proteomics
No full textThe unbiased identification of less-abundant transcription factors, which direct the expression of a target gene, is technically challenging. Here, we present a protocol to analyze the locus-specific chromatin-regulating proteome using in situ capture of chromatin interactions by an inactive Cas9 (dCas9). We describe steps for designing guide RNAs and transfection, followed by precipitation of chromatin and associated proteins. In the last step, we describe the elution of DNA and proteins for PCR and mass spectrometric analysis, respectively.
For complete details on the use and execution of this protocol, please refer to Alkhayer et al.Gefördert durch den Open-Access-Publikationsfonds der UB Marburg
Comparative Visualization of Large Tabular Data
No full textAuthor Reem Hourieh, BScZusammenfassungen in deutscher und englischer SpracheMasterarbeit Universität Linz 201
Comparative Visualization of Large Tabular Data
No full textAuthor Reem Hourieh, BScZusammenfassungen in deutscher und englischer SpracheMasterarbeit Universität Linz 201
Protocol to target a promoter region in human embryonic kidney cells using the CRISPR-dCas9 system for single-locus proteomics
No full textSummary: The unbiased identification of less-abundant transcription factors, which direct the expression of a target gene, is technically challenging. Here, we present a protocol to analyze the locus-specific chromatin-regulating proteome using in situ capture of chromatin interactions by an inactive Cas9 (dCas9). We describe steps for designing guide RNAs and transfection, followed by precipitation of chromatin and associated proteins. In the last step, we describe the elution of DNA and proteins for PCR and mass spectrometric analysis, respectively.For complete details on the use and execution of this protocol, please refer to Alkhayer et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics
Nanosizing Cynomorium: Thumbs up for Potential Antifungal Applications
No full textCynomorium coccineum L., the desert thumb, is a rather exotic, parasitic plant unable to engage in photosynthesis, yet rich in a variety of unique compounds with a wide spectrum of biological applications. Whilst extraction, separation and isolation of such compounds is time consuming, the particular properties of the plant, such as dryness, hardness and lack of chlorophyll, render it a prime target for possible nanosizing. The entire plant, the external layer (coat) as well as its peel, are readily milled and high pressure homogenized to yield small, mostly uniform spherical particles with diameters in the range of 300 to 600 nm. The best quality of particles is obtained for the processed entire plant. Based on initial screens for biological activity, it seems that these particles are particularly active against the pathogenic fungus Candida albicans, whilst no activity could be observed against the model nematode Steinernema feltiae. This activity is particularly pronounced in the case of the external layer, whilst the peeled part does not seem to inhibit growth of C. albicans. Thanks to the ease of sample preparation, the good quality of the nanosuspension obtained, and the interesting activity of this natural product, nanosized coats of Cynomorium may well provide a lead for future development and applications as “green” materials in the field of medicine, but also environmentally, for instance in agriculture
Reem Ali’s Zabad (2008): Cinematic Dissidence in Syria
No full textStefanie Van de Peer - ORCID: 0000-0003-3152-2912
https://orcid.org/0000-0003-3152-2912Item not available in this repository.The documentary camera in Syria is emerging as one of the most powerful tools in raising awareness during this current revolution. While foreign media have been banned from the country, revolutionaries and ordinary Syrians are capturing events and people on their small digital and other cameras in order to testify about their struggle. This attests to the power of the image and to the political effectiveness of the documentary. This article briefly covers the nature of political film- and documentary-making in Syria. The author points out the contribution of Omar Amiralay and Hala al-Abdallah to this trend and specifically deals with a young woman’s documentary that subtly and moderately engages with politics and family matters. Reem Ali’s 2008 documentary Zabad shows that negotiations with dissidence and political repression take place on many different levels, and that the uprisings are not only due to sudden changes leading to “democratic” sensibilities in reaction to contextual factors and enabled through neighbouring practices. In fact, artistic dissidence comes as a consequence of numerous dissident activities that have already been reacting to years of institutional and social repression.http://dx.doi.org/10.1080/14797585.2012.64770916pubpub2-
Rethinking public space in the city of Jeddah, Saudi Arabia
No full textThesis (M. Arch.)--Massachusetts Institute of Technology, Dept. of Architecture, 2012.This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.Cataloged from student-submitted PDF version of thesis.Includes bibliographical references (p. 139).Can urban intervention affect social behaviors within a city? And if so, what happens when that city is partial to cultural restrictions? The target in question here is the city of Jeddah, Saudi Arabia. The dilemma is that the city has no true form of public space; despite having spaces designated as such. The lack of public space can be related to two main aspects linked to the nature of the city: 01. Gender Segregation: Questioning what actually IS public if both males and females can't access these spaces freely. 02. The development of the city since the oil boom: Looking at social, economic, demographic and urban ramifications. My thesis serves as a contribution to the social needs of the Saudi society in Jeddah today. By questioning the issue of 'publicness' I intend to analyze how the development of the city has been affected by the cultural restrictions imposed upon it.by Reem Abuzeid.M.Arch
New Mexico pecan production
No full textPresented at Urbanization of irrigated land and water transfers: a USCID water management conference on May 28-31, 2008 in Scottsdale, Arizona.Pecans are a major agricultural crop in New Mexico. Currently there are approximately 11,000 hectares of pecans in the Elephant Butte Irrigation District, consuming more than one third of the annual diversion. The research presented here provides previously unavailable broad-scale estimates of pecan ET and pecan yield response to water. The data at the foundation of this paper were generated using the Regional ET Estimation Model (REEM) developed at New Mexico State University for agricultural and riparian vegetation (Samani et al. 2005, 2006, 2007). REEM uses remotely sensed satellite data to calculate ET as a residual of the energy balance. This research extends the results of REEM to an analysis of yield response to water in irrigated pecan production in the EBID. The study region is rapidly urbanizing and experiencing growing competition for scarce surface and groundwater supplies. The results of this research provide new insight into pecan water use and yields. This research illustrates the linkages that can be made between remote sensing technology, farm-level water management, and yield outcomes. This research sheds new light on the long-standing practice of deficit irrigation in pecans, the yield and conservation impacts of this practice, as well as water conservation policy implications
The moderation of creative dissidence in Syria: Reem Ali’s documentary Zabad
No full textThe documentary camera in Syria is emerging as one of the most powerful tools in raising awareness during this current revolution. While foreign media have been banned from the country, revolutionaries and ordinary Syrians are capturing events and people on their small digital and other cameras in order to testify about their struggle. This attests to the power of the image and to the political effectiveness of the documentary. This article briefly covers the nature of political film- and documentary-making in Syria. The author points out the contribution of Omar Amiralay and Hala al-Abdallah to this trend and specifically deals with a young woman’s documentary that subtly and moderately engages with politics and family matters. Reem Ali’s 2008 documentary Zabad shows that negotiations with dissidence and political repression take place on many different levels, and that the uprisings are not only due to sudden changes leading to “democratic” sensibilities in reaction to contextual factors and enabled through neighbouring practices. In fact, artistic dissidence comes as a consequence of numerous dissident activities that have already been reacting to years of institutional and social repressio
Nuevos espacios femeninos en la narrativa egipcia actual: Reem Bassiouney
No full textIn the 21st century, the Egyptian women fiction is a critical review of the prevailing social order. The core topic is usually the slowdown in the process of social change because of the pressure exerted by the traditional dominant system. In this article we will address how the Egyptian writer, Reem Bassiouney (Alexandria, 1973), in her novel Professor Hana, projects an image of today’s Arab woman in search of her own space and out of traditional roles. In this context, social, personal and gender conflicts create frustrated, insecure, and isolated characters, regarding the immutability of the situation or the weakness or failure to bring about change. The aim is to show that literature is used by this Arab writer as a tool to raise awareness of current problems. Like some other Arab women writers, she uses literary fiction to approach gender relations as well as the attitudes of men and women within a traditionally androcentric society which assigns each of them a particular role in a specific space. Bassiouney uses sarcasm, irony and humour as weapons against a society which hinders the integration of some of its individuals because of their behaviour and aspirations. It is also clear that the Egyptian author belongs to the new generation of authors who do not attempt to present their environment in a realistic manner, but they rather use their experiences and memories to construct the scenario of their weariness with their surroundings. As a result, it is confirmed that, through literature, today Arab women are claiming a space of their own to make their situation visible and to gain access to the public framework by generating opinion.En el siglo XXI, la narrativa femenina egipcia es una literatura crítica con el orden social imperante. El tema medular suele ser la ralentización del proceso de cambio social a causa de la presión ejercida por el sistema tradicional dominante. En este artículo abordaremos cómo la escritora egipcia Reem Bassiouney (Alejandría, 1973), en su novela Profesora Haná, proyecta una imagen de la mujer árabe actual fuera de los moldes tradicionales en busca de un espacio propio. En este contexto, los conflictos sociales, personales y de género están en la base de personajes frustrados, inseguros y aislados ante la inmutabilidad de la situación o ante la debilidad o el fracaso para provocar un cambio. El objetivo es mostrar que la literatura es utilizada por la escritora árabe como una herramienta para concienciar sobre los problemas actuales. Al igual que otras escritoras árabes, recurre a la ficción literaria para abordar las relaciones de género y la actitud de hombres y mujeres en el marco de una sociedad tradicionalmente androcéntrica que le asigna a cada uno un papel concreto en un espacio definido. Bassiouney utiliza el sarcasmo, la ironía y el humor como armas contra una sociedad que dificulta la integración de algunos de sus individuos por su comportamiento y aspiraciones. Se constata también que la autora egipcia se inscribe en la nueva generación de autores que no procuran la presentación de su entorno de una forma realista, sino que se sirven de sus vivencias y recuerdos para construir el escenario de su hastío ante lo que les rodea. Como resultado se confirma que, a través de la literatura, la mujer árabe actual reclama un espacio propio para hacer visible su situación y para acceder al espacio público generando opinión
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