7 research outputs found
Effective dosage of growth differentiation factor‐9β in folliculogenesis and angiogenesis in the sheep ovarian tissues grafted onto chick embryo chorioallantoic membrane
No full textAim: Scientists have tried to culture and transplant the ovarian tissues (OT), to preserve fertility in cancer
patients. However, one of the main limitations to the applicability of this technique is the folliculogenesis
disruption after transplantation. Due to the roles exerted by growth differentiation factor-9β (GDF9β), we
decided to determine the most effective dose of GDF9β on promotion of folliculogenesis and angiogenesis in
sheep OT grafted onto the chick chorioallantoic membrane (CAM).
Methods: Fresh sheep OT were grafted onto the CAM for 5 days, and divided into four groups based on
the addition of increasing doses of GDF9β (0, 150, 200 and 250 ng/mL). Following culture, histological
(hematoxylin and eosin [H&E] staining) and immunohistological studies (Ki-67) were done. Fibrotic and
necrotic regions were measured using MICROVISIBLE software. For comparing the follicle development
rates between the groups as well as differences in the Ki-67-positive follicles, analysis of variance was
applied.
Results: In both 200 and 250 ng/mL GDF9β groups, significantly higher rates of intermediary and primary follicles were observed, also the numbers of good quality follicles increased in the aforementioned
groups and the rates of fibrotic and necrotic areas decreased. Moreover, in the 200 and 250 ng/mL
GDF9β groups, the number of capillaries and the proliferative activity increased. The lower dose of
GDF9β (150 ng/mL) neither activated the primordial follicles nor lead to an increase in the number of
growing follicles.
Conclusion: Addition of high dosages of GDF9β to the OT, grafted onto the CAM resulted in higher
folliculogenesis and better transplantation features due to improvement in angiogenesis
Measuring Customer Satisfaction Using Multi-Criteria Analysis Model of Customer Satisfaction to Evaluate Product Lines (Case Study: Kaveh Glass Industrial Group)
No full textToday, customer retention and growth is the first goal. Companies have taken a heavy toll on each customer, and competitors are constantly seeking to steal them. Satisfied customers have many benefits for the company, they are less sensitive to prices, will be a customer for a long period of time, and give a favorable view of the company and its products and services to others. Paying attention to the factors that are less important from the customer's point of view and evaluating these factors leads to spending resources in areas that have less impact on customer behavior, so understanding the factors that have the greatest impact on customer satisfaction to the organization to move in this will help. In this study, we intend to use the operational data of the lines and customer satisfaction scales to evaluate the relative efficiency of product lines using the network data envelopment analysis method. In order to achieve this goal and quantify the data obtained from the questionnaire, another linear programming method called multi-criteria satisfaction analysis will be used. Another innovation of this research is the application of customer expectations in the data envelopment analysis mole because most researchers that focus on quality do not consider this component in their evaluation model. The results indicate that the distribution system is the most effective factor and resources and supply is the most effective factor affecting customer satisfaction
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Author Correction: Discovery of widespread transcription initiation at microsatellites predictable by sequence-based deep neural network (Nature Communications, (2021), 12, 1, (3297), 10.1038/s41467-021-23143-7)
No full textEffect of air pollution on disease burden, mortality, and life expectancy in North Africa and the Middle East: a systematic analysis for the Global Burden of Disease Study 2019
No full textEarly versus Delayed Switching from Controlled to Assisted Ventilation: A Target Trial Emulation
No full textRationale: In critically ill patients receiving invasive mechanical ventilation, switching from controlled to assisted ventilation is a crucial milestone toward ventilator liberation. The optimal timing for switching to assisted ventilation has not been studied. Objectives: Our objective was to determine whether a strategy of early compared with delayed switching affects the duration of invasive mechanical ventilation, ICU length of stay, and mortality. Methods: We conducted a target trial emulation using the prospective, global WEAN SAFE (the WorldwidE AssessmeNt of Separation of pAtients From ventilatory assistancE) dataset. Patients were eligible for switching if they were still on controlled mechanical ventilation, were not receiving neuromuscular blockers, and had PaO2:FIO2 ratios .150 mm Hg. We compared an “early switching” strategy (switch within 1 day after reaching switching eligibility criteria) with a “delayed switching” strategy (switch 1 or more days after reaching the switching eligibility criteria). The primary outcome was the 28-day cumulative incidence of successful extubation. Secondary outcomes included 28-day and 90-day ICU discharge and ICU mortality. Measurements and Main Results: A total of 1,489 patients met the switching eligibility criteria. The early-switch group had, on average, 4 additional days of being successfully extubated over the 28-day period (95% confidence interval [CI], 3–6 days; P, 0.001) compared with the delayed group, with a higher difference in cumulative incidence of successful extubation at Day 28 (7% [95% CI, 0–13%]; P = 0.04). Early switching was associated with an 11% higher cumulative incidence of ICU discharge at Day 28 (95% CI, 7–18%; P, 0.001) and an average of 7 additional days discharged from the ICU over the 90-day period (95% CI, 4–12 days; P, 0.001) compared with delayed switching. ICU mortality rates did not differ between the strategies. Conclusions: Early switching from controlled to assisted ventilation is associated with shorter duration of invasive mechanical ventilation and ICU stay compared with delayed switching
Weaning from mechanical ventilation in intensive care units across 50 countries (WEAN SAFE): a multicentre, prospective, observational cohort study
No full textBackground: Current management practices and outcomes in weaning from invasive mechanical ventilation are poorly understood. We aimed to describe the epidemiology, management, timings, risk for failure, and outcomes of weaning in patients requiring at least 2 days of invasive mechanical ventilation. Methods: WEAN SAFE was an international, multicentre, prospective, observational cohort study done in 481 intensive care units in 50 countries. Eligible participants were older than 16 years, admitted to a participating intensive care unit, and receiving mechanical ventilation for 2 calendar days or longer. We defined weaning initiation as the first attempt to separate a patient from the ventilator, successful weaning as no reintubation or death within 7 days of extubation, and weaning eligibility criteria based on positive end-expiratory pressure, fractional concentration of oxygen in inspired air, and vasopressors. The primary outcome was the proportion of patients successfully weaned at 90 days. Key secondary outcomes included weaning duration, timing of weaning events, factors associated with weaning delay and weaning failure, and hospital outcomes. This study is registered with ClinicalTrials.gov, NCT03255109. Findings: Between Oct 4, 2017, and June 25, 2018, 10 232 patients were screened for eligibility, of whom 5869 were enrolled. 4523 (77·1%) patients underwent at least one separation attempt and 3817 (65·0%) patients were successfully weaned from ventilation at day 90. 237 (4·0%) patients were transferred before any separation attempt, 153 (2·6%) were transferred after at least one separation attempt and not successfully weaned, and 1662 (28·3%) died while invasively ventilated. The median time from fulfilling weaning eligibility criteria to first separation attempt was 1 day (IQR 0-4), and 1013 (22·4%) patients had a delay in initiating first separation of 5 or more days. Of the 4523 (77·1%) patients with separation attempts, 2927 (64·7%) had a short wean (≤1 day), 457 (10·1%) had intermediate weaning (2-6 days), 433 (9·6%) required prolonged weaning (≥7 days), and 706 (15·6%) had weaning failure. Higher sedation scores were independently associated with delayed initiation of weaning. Delayed initiation of weaning and higher sedation scores were independently associated with weaning failure. 1742 (31·8%) of 5479 patients died in the intensive care unit and 2095 (38·3%) of 5465 patients died in hospital. Interpretation: In critically ill patients receiving at least 2 days of invasive mechanical ventilation, only 65% were weaned at 90 days. A better understanding of factors that delay the weaning process, such as delays in weaning initiation or excessive sedation levels, might improve weaning success rates. Funding: European Society of Intensive Care Medicine, European Respiratory Society
