1,354,581 research outputs found
A comparison of procedures for structural learning of biological networks
No full textOver the past years, microarray technologies have produced a tremendous amount of gene expression data. The availability of these data has motivated researchers to assess genes function and to gain a deeper understanding of the cellular processes, using network theory as tool for the analysis. An elegant framework for modeling and inferring network structures in biological systems is provided by graphical models. They allow the stochastic description of network associations and dependence structures in complex highly structured data. However, typically gene expression data set includes a large number of variables but only few samples making standard graphical model theories inapplicable. The issues presented by genetic data have led to further extend the theory of graphical models to allow their applications in this area. The main aim of this thesis is the comparison of recent procedures, which estimate sparse concentration matrices and learn the structure of biological networks, through the use of both simulated and real data. The compared procedures are: G-Lasso algorithm (Friedman et al., 2008), Shrinkage estimator with empirical Bayes approach for model selection (Schafer and Strimmer, 2005a, 2005b), PC-algorithm (Kalisch and Buhlmann, 2007). When n > p, we consider also the simple frequentist approach based on MLE and t-test for model selection (see Lauritzen, 1996). Regarding the simulated data, for having a realistic simulation of the biological structures, the data have the peculiarity to reproduce few gene regulatory network structures of interest and they are generated by exploiting some properties of the Cholesky decomposition of a matrix. Concerning the real data, we consider the analysis of one of the best characterized system: Escherichia coli. A large part of its transcriptional regulatory network is known, hence it can be used as a gold-standard to assess the performance of different procedures in the comparative study.Negli ultimi anni, le tecnologie dei microarray hanno prodotto una grande quantità di dati provenienti da processi di espressione genica. La disponibilità di questi dati ha permesso ai ricercatori di poter approfondire lo studio della funzione dei diversi geni e poter acquisire una più profonda conoscenza sui processi cellulari, utilizzando come strumento di ricerca la teoria dei network. I modelli grafici risultano essere un utile strumento per la modellazione e l'analisi delle strutture dei networks derivanti da dati biologici. Infatti, questi modelli consentono di rappresentare in modo stocastico le associazioni e le strutture di dipendenza tra gli elementi di data set con struttura complessa. Tuttavia, i dati derivanti da profili di espressione genica si presentano con un elevato numero di variabili ma solo poche osservazioni rendendo, perciò, la teoria classica dei modelli grafici inapplicabile. I problemi legati all'utilizzo di dati genetici hanno portato ad estendere la teoria dei modelli grafici per consentire l'impiego di questi modelli anche in questo campo di applicazione. Lo scopo principale di questa tesi è quello di confrontare, attraverso l'utilizzo di dati simulati e reali, recenti procedure sviluppate con lo scopo di stimare matrici di concentazione sparse e ricostruire i networks biologici. Le procedure considerate per il confronto sono: l'algoritmo G-Lasso (Friedman et al., 2008), lo stimatore Shrinkage associato con l'approccio Bayes empirico per la selezione del modello (Schafer and Strimmer, 2005a, 2005b), l'algoritmo PC (Kalisch and Buhlmann, 2007). Quando n > p, consideriamo anche un semplice approccio frequentista basato sullo stimatore ML e l'utilizzo del test t per la selezione del modello (si veda Lauritzen, 1996). Per quanto riguarda i dati simulati, per avere strutture biologiche simili a quelle reali, i dati hanno la peculiarità di riprodurre alcune strutture dei network di regolazione genica e sono ottenuti sfruttando alcune proprieta’ della decomposizione di Cholesky di una matrice. Per il confronto con dati reali, sono stati utilizzati dati derivanti da uno dei sistemi maggiormente studiati: Escherichia coli. Infatti, grand parte del network di regolazione genica di questo battere è noto, quindi può essere utilizzato come riferimento per valutare il rendimento delle diverse procedure poste a confronto
Larvicidal activity and influence of bacillus thuringiensis Var. israelensis on aedes albopictus oviposition in ovitraps during a two-week check interval protocol
No full textToxicity persistence of Bacillus thuringiensis var. israelensis (Bti) was evaluated in laboratory and field trials to develop a new protocol for Aedes albopictus monitoring. In the laboratory, the residual toxicity of 5 Bti formulations was compared using the percentage mortality at days 4, 8, 12, and 16 of Culex pipiens larvae exposed for 24 h to Bti treatments. The results showed a good performance of all tested formulations (>97 mortality at day 14, for all the formulations), but only VectobacTM 12AS at the concentration of 1 ml/liter showed an efficacy of 100 for 2 wk. A field study was designed to test the effect of Bti on the ovitrap check interval or influence of ovipositional response of gravid Ae. albopictus females. Three different ovitrap treatments were used: ovitraps with tap water checked weekly; ovitraps with tap water checked every 2 wk; ovitraps with Bti (Vectobac 12AS, dose of 1 ml/liter) checked every 2 wk. Our study demonstrated that in the ovitrap, the toxic action of a 1 solution of Bti was maintained for at least 14 days with mortality of 100 and that rainfall did not seem to negatively influence the residual action of Bti. Therefore the probability that the larvae may complete the developmental cycle in ovitraps with Bti seems to be very low. The oviposition activity index showed that Bti enhances the oviposition rate of Ae. albopictus by 17.4. © 2009 by The American Mosquito Control Association, Inc
Apresentação
No full textComo citar:
TARTUCE, Gisela Lobo; ALMEIDA, Patrícia Albieri. Apresentação. In: FUNDAÇÃO CARLOS CHAGAS. Prêmio Professor Rubens Murillo Marques 2023: experiências docentes em licenciaturas. São Paulo: FCC, 2023. p. 7-8. (Textos FCC: Relatórios técnicos, 65).
Tartuce, G. L., & Almeida, P. A. (2023). Apresentação. In Fundação Carlos Chagas, Prêmio Professor Rubens Murillo Marques 2023: Experiências docentes em licenciaturas (Textos FCC: Relatórios técnicos, 65, pp. 1-8). FCC
Control device for a refrigerating machine
No full textThe present invention concerns a control device for a refrigerating machine according to claim 1. In particular, the present invention finds useful, but not exclusive, application in the regulation of the delivery
temperature of a service fluid in output from a water chiller for centralized air-conditioning systems, to which the following
description shall make explicit reference without, however, any loss of generality
Positive Narrative Therapy for an Unspecified Eating Disorder: A Child Case Report
No full textA new sequential multicomponent treatment (cognitive behavioral therapy followed by well-being-enhancing narrative strategies) was applied for an unspecified eating disorder in a 10-year-old boy for reducing symptoms and improving well-being. Both distress and well-being were assessed with self and observer ratings at baseline, posttreatment, and 3-, 6-, 12-month follow-up. An ABA design was used and reliable change indexes were calculated as outcome measures. This new sequential psychotherapeutic approach, which integrated standard cognitive behavioral therapy with narrative strategies addressed at promoting well-being in a child patient diagnosed with an unspecified eating disorder, was able to reduce symptoms and to foster his resources and positive behaviors. The patient himself, his parents, and teachers confirmed these improvements. This case report shows the clinical usefulness of a new sequential psychotherapeutic approach for treating an unspecified eating disorder in a child. It provides psychotherapists with relevant clinical implications concerning the emotional and interpersonal dimensions involved in this disabling condition
Method for estimation the thermal load of a circuit for a service fluid at outlet from a refrigerating machine
No full textOnce upon a time: A school positive narrative intervention for promoting well-being and creativity in elementary school children
No full textRecent research highlighted the importance of incorporating programs for promoting well-being and creativity in schools. However, eudaimonic well-being received only limited attention and only few interventions aimed at its promotion in the school setting. This research aimed to compare the efficacy of an intervention based on storytelling and narrative techniques versus a control condition for the promotion of well-being and creativity in elementary schoolchildren. A total of 165 students (78 girls, 87 boys; Mage = 9.3 years; SD = 0.5) were randomized to a School Positive Narrative Intervention or to a controlled condition. Children were assessed before and after intervention and at 3-month follow-up with self-reports of well-being, anxiety, depression and somatization. A storytelling task was implemented, and specific creativity storytelling scores were calculated for the stories produced by children during the intervention. At post intervention, children assigned to the narrative intervention reported increased levels of well-being and decreased depression, anxiety, and somatization, compared to controls. These improvements were maintained at 3-month follow-up. Higher scores on creativity emerged in stories focused on fear, sadness, and happiness. The use of narrative strategies help children to identify their personal resources, to express creativity, and to assimilate the concept of eudaimonic well-being that could be difficult to process because of its abstractness and multidimensional nature. This brief intervention fostered children creativity and it produced beneficial effects on children's well-being and distress
Method for regulating the delivery temperature of a service fluid in output from a refrigerating machine
No full textGoing Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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