486 research outputs found
Motion detection technology as a tool for cardiopulmonary resuscitation (CPR) quality improvement
No full textThe most popular method of training in basic life support and AED remains instructor-led training courses. Recent reviews provide good evidence to support alternative methods of training including lay instructors, self-directed learning (web, video, poster) and CPR feedback/prompt devices
MAXILLOFACIAL SURGERY SIMULATION FROM 3D CT IMAGES
No full textThe purpose of the project is to analyze Simplant CMF software (Materialise, Leuven, Belgium) for planning the aesthetic impact of the more frequent osteotomies used to correct dentoskeletal malocclusions, such as maxillary hypoplasia and mandibular progranthism. The software allows the surgeon to plan treatment accurately and to predict the soft tissue behaviour realistically to evaluate the aesthetic result
Assembly, annotation, and read counts for 2016 Sargasso Sea incubations
No full textData sets for:
Lampe RH, Wang S, Cassar N, and Marchetti A. (in prep) Strategies among phytoplankton in response to alleviation of nutrient stress in a subtropical gyre.
File descriptions:
site1_clust99.fa.gz - Assembled contigs for site 1 after clustering based on 99% similarity.
site2_clust99.fa.gz - Assembled contigs for site 2 after clustering based on 99% similarity.
site1_results.tsv.gz - annotations and counts for each contig from site 1 (tab-delimited)
site2_results.csv.gz - annotations and counts for each contig from site 2 (tab-delimited)
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Ferritin Gene Expression among Pennate and Centric Diatoms
No full textThe ability to store iron through the use of iron storage proteins, ferritins, has evolved as an advantageous strategy for numerous diatom species. Iron is a micronutrient in the ocean that typically exists at low concentrations throughout much of the marine system. Therefore, iron deficiency often plays a central role in controlling primary productivity, and thus, diatom populations within the ocean (Martin, 1990). This project investigates the expression of an iron storage gene, ferritin, in three pennate diatoms and four centric diatoms. The strains were cultured at Bigelow Laboratory for Ocean Sciences and the University of North Carolina at Chapel Hill (UNC) and grown as triplicate cultures under various iron conditions. This experiment allowed for the comparison of ferritin gene expression along with iron storage capabilities, in contrasting iron environments among phylogenetically distinct diatoms. This was achieved by utilizing the ΔCt method. Our results suggest that pennates may upregulate ferritin levels in high iron environments. Marchetti et al (2009) similarly demonstrated that pennates such as Pseudo-nitzschia granii store iron during temporary pulses, which allows them to continue to proliferate once iron limited conditions return. Two of the examined centrics, however, demonstrated greatest ferritin gene expression under low iron conditions. This result is counterintuitive, as ferritin is a storage gene and high expression during low iron levels appears inefficient. However, this finding supports the theory that centrics may be using their ferritin as an iron buffer and thus eludes to physiological differences in the way these two diatom lineages are storing iron and utilizing their ferritin genes. There is also evidence of a biogeographical distinction among ferritin use in certain species. Diatoms in open ocean regions, where iron is often limited, may be utilizing their ferritin differently than those persisting in high-iron coastal areas.Bachelor of Scienc
DOSSIER: TEN YEARS
No full textA collection of articles and video essays dedicated to the series of political omnibus films TEN REARS (Hong Kong, Japan, Thailand, Taiwan), with text and video contributions from a variety of international academics, producers, distributors and critics. Author, with Gina Marchetti, of introductory article. International contributions by Clarence Tsui, Andrew Choi, Felix Tsang, Laikwan Pang, K.C. Lo, Ruby Cheung, Jenny Coates, Timmy Cheng, Vivian Lee, Anchalee Chaiworaporn
DOSSIER: TEN YEARS
No full textA collection of articles and video essays dedicated to the series of political omnibus films TEN REARS (Hong Kong, Japan, Thailand, Taiwan), with text and video contributions from a variety of international academics, producers, distributors and critics. Author, with Gina Marchetti, of introductory article. International contributions by Clarence Tsui, Andrew Choi, Felix Tsang, Laikwan Pang, K.C. Lo, Ruby Cheung, Jenny Coates, Timmy Cheng, Vivian Lee, Anchalee Chaiworaporn
The impact of renewable energy systems on local sustainability
No full textThe purpose of the paper is to discuss and assess the impact of renewable energy, RE, generation on sustainable communities, with a specific focus on employment impact. In recent years, local authorities have been involved in the energy planning process due to the site-specific nature of renewable production and thanks to their nearer connection to citizens. Four small and medium-size sustainable communities belonging to Marche region, in central Italy, have been analysed. The work forecasts the employment generated by the RE capacity defined by two different energy scenarios: 1) the 'business as usual' scenario with a 20% CO2 emission reduction target by 2020, where all the energy policies suggested by Sustainable Energy Action Plans have been considered; 2) the 'potential' scenario with a 50% CO2 emission reduction target by 2050. Results show that the spreading of renewable energy, as expected, can increase local jobs, although the high investments required and problems connected to the impact on the grid have to be considered in the analysis of the global economic effect
Intact polar diacylglycerol (IP-DAG) lipids measured in cultures of four Antarctic diatom isolates
No full textDiatoms were isolated by Adrian Marchetti (http://orcid.org/0000-0003-4608-4775) from waters of the Bellingshausen Sea and grown on replete media. Cells were collected onto 0.7 µm GF/F filters. Extraction was performed using a modified Bligh and Dyer (Bligh and Dyer, 1959; https://doi.org/10.1139/o59-099) method described in Popendorf et al. (2013; https://doi.org/10.1007/s11745-012-3748-0). Lipid extracts were analyzed by HPLC-ESI-MS with data dependent-MS2 acquisition on a high-resolution, accurate mass Thermo Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (ThermoFisher Scientific, Waltham, MA, USA) coupled to an Agilent 1200 HPLC system (Agilent, Santa Clara, CA, USA). The HPLC-ESI-MS method is described in Collins et al., 2016 (https://doi.org/10.1021/acs.analchem.6b01260).
The LOBSTAHS lipidomics discovery software (Collins et al., 2016; https://doi.org/10.1021/acs.analchem.6b01260) was used to putatively identify HPLC-MS features in the data. We confirmed each LOBSTAHS identification using two additional means: (1) via comparison of data-dependent MS2 spectra with those from authentic standards or published reference spectra and (2) by requiring the presence of the same compound identity in data acquired in the opposite HPLC-ESI-MS ionization mode. We confirmed all LOBSTAHS identities at the lipid class level (e.g., PC versus PE, or MGDG versus TAG) using a new, experimental LOBSTAHS feature which automatically detects diagnostic product ion fragments and constant neutral losses (as given in Popendorf et al., 2013; https://doi.org/10.1007/s11745-012-3748-0) in the available data-dependent MS2 spectra for each sample. After identification, quantification of analytes was performed using a series of standard curves, followed by normalization to concentration of an internal standard.
Lipid identities are resolved only to the level of bulk fatty acid composition (i.e., the sum of the properties of the substituents at both the sn-1 and sn-2 positions). As a result, in some cases (e.g., PC 40:8), several different molecules having the same bulk fatty acid composition have been identified. These can be distinguished by retention time, which is given in the "Comment" field for each entry
Prospettive sulla valorizzazione degli olii estratti dalle piante come fonte di materie prime per l'industria chimica: leEuphorbiedella Sardegna
No full textIn the present paper the author describes the research carried out at the University of Sassari on the utilization as fuels or chemical feedstocks of oils derived from plants. Examination of the results obtained with oils derived fromEuphorbiaevegetanting in Sardinia suggests to orient the studies toward the isolation of fine chemicals rather then toward the utilization as fuels, directly
or after pyrolysis. This conclusion is supported by the
comparison of data on three SardinianEuphorbiaeand by considerations on the Sardinian land
Migration and Domestic Work
No full textThis open access short reader offers a systematic overview of the scholarly debate on the experiences of migrant domestic workers at a global level, in the past as well as in present time. It tackles the nexus between migration and domestic work with a multi-layered approach. The book looks into the issue of (paid) domestic work in migratory contexts by investigating the feminization of migration, thereby considering the larger framework within which this specific phenomenon takes place. The author explains notions such as the “international division of reproductive labor” or “global care chains” which emphasize the inequality in the way care and domestic tasks are distributed today between middle-class women in receiving nations and migrant domestic workers. Moreover, the book shows how women migrating to work in the domestic work and private care sector are facing a complex landscape of migration and labor regulations that are extremely difficult to navigate. At the same time, this issue also addresses employers’ households who cannot find appropriate or affordable care among declining welfare states and national workers reluctant to take the job, whilst legal regulations make difficult to hire a domestic worker who is a third country national. As such this book offers an interesting read to academics, policy makers and all those working in the field
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